Article
Food Science & Technology
Lalita Chotphruethipong, Thunwa Binlateh, Pilaiwanwadee Hutamekalin, Rotimi E. Aluko, Surapun Tepaamorndech, Bin Zhang, Soottawat Benjakul
Summary: The study demonstrates that hydrolyzed collagen derived from defatted sea bass skin can promote proliferation and differentiation of preosteoblast cells, enhance alkaline phosphatase activity and mineralization, increase calcium depositions, and elevate the expressions of RUNX2 and p-Akt, suggesting its potential as a functional ingredient for promoting bone strength.
Article
Biochemistry & Molecular Biology
Joo Hyun Jang, Singeun Kim, Hyun Jung Lee, Hyung Joo Suh, Kyungae Jo
Summary: The study demonstrated that WPH significantly increased alkaline phosphatase activity and calcium deposition, while upregulating factors related to osteoblast differentiation. Furthermore, in rats administered with a high dose of WPH, significant increases were observed in ALP and IGF-1 levels, tibia length, and growth plate height, indicating a growth-promoting effect on bone.
Article
Chemistry, Physical
Hanbai Wu, Yi Liu, Chen Ai, Shuo Shi, Raymond H. W. Lam, Jinlian Hu
Summary: This study investigates the effects of cell-scale microstructures on cell differentiation and the underlying mechanisms. The results demonstrate that microstructures with a curvature radius of 50 μm can provide maximum acting forces to cells, promoting cell spreading and osteoblast differentiation of MC3T3-E1 cells. Furthermore, the two-dimensional force generated by narrow curvatures on microgrooves outperforms other microstructures in terms of osteogenesis of MC3T3-E1 cells.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Biochemistry & Molecular Biology
Sarah A. Schoonraad, Michael L. Trombold, Stephanie J. Bryant
Summary: The study demonstrated the successful chemical attachment of BMP-2 to a polyethylene glycol hydrogel using thiol-norbornene click chemistry to enhance osteogenesis. Tethered BMP-2 augmented the osteogenic response of MC3T3-E1 preosteoblasts, leading to significant increases in osteomarkers Bglap and Ibsp. This work also identified the role of dexamethasone in inducing Ibsp expression and confirmed the activation of the BMP canonical signaling pathway by tethered BMP-2.
Article
Pharmacology & Pharmacy
Daichi Nagashima, Yukiko Ishibashi, Sachiko Kawaguchi, Megumi Furukawa, Masahiro Toho, Megumi Ohno, Takeaki Nitto, Nobuo Izumo
Summary: Lactoferrin can promote osteoblast proliferation and suppress bone resorption. The mechanisms of human lactoferrin on osteoblast differentiation are still unclear, and most studies have used bovine lactoferrin. This study aimed to compare the effects of bovine lactoferrin and human recombinant lactoferrin on MC3T3-E1 osteoblast cells. The results showed that both lactoferrins enhanced MC3T3-E1 cell differentiation and calcification.
Article
Chemistry, Multidisciplinary
Han Liu, Lin Qiu, Hao Liu, Fengji Li, Yaru Fan, Lulu Meng, Xiaoqian Sun, Chaojun Zhan, Rui Luo, Chao Wang, Jun Zhang, Ruixin Li
Summary: This study utilized low-temperature 3D printing technology to construct a silk fibroin/collagen/hydroxyapatite composite scaffold, designed with different fiber cross-angle structures and subjected to various performance tests and cellular experiments. The results indicated that the 90 degrees fiber cross-angle structure significantly enhanced the mechanical properties of the scaffold, promoting cell proliferation and osteogenesis, providing insights into scaffold properties and applications.
Article
Chemistry, Applied
Liwei Qi, Kangyu Wang, Jiaojiao Zhou, Hongru Zhang, Yujie Guo, Chunhui Zhang
Summary: This study investigated the effect of phosphorylation modification on collagen peptide's calcium-binding capacity and pro-mineralization activity. The results showed that phosphorylation significantly enhanced calcium-binding capacity, primarily through carbonyl, carboxyl, and phosphate groups. Molecular docking confirmed that phosphorylation increased calcium-binding capacity through ionic and coordination bonds. Cell experiments demonstrated that phosphorylated collagen peptide-calcium complex significantly improved mineralization activity.
Article
Medicine, Research & Experimental
Jian Yang, Kai Yu, Dongmei Liu, Jie Yang, Li Tan, Dianying Zhang
Summary: The study showed that irisin can promote osteogenic differentiation in MC3T3-E1 cells by enhancing alkaline phosphatase activity and mineralized nodule formation, and upregulating the expression of several osteogenic genes. This suggests that irisin may be a potential therapeutic agent for osteoporosis.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Integrative & Complementary Medicine
Weiye Cai, Bin Sun, Chao Song, Fei Liu, Zhengliang Wu, Zongchao Liu
Summary: Resveratrol enhances osteogenesis in mice by promoting MC3T3-E1 cell proliferation and differentiation. Although the exact mechanism is unclear, increased autophagy may play a role in this process.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Biochemistry & Molecular Biology
Makoto Izumiya, Miyu Haniu, Katsuya Ueda, Haruka Ishida, Chuang Ma, Hirokazu Ideta, Atsushi Sobajima, Koki Ueshiba, Takeshi Uemura, Naoto Saito, Hisao Haniu
Summary: The study evaluated the osteogenesis of MC3T3-E1 cells under different culture media conditions, measuring cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification. The research found that the type of base medium, the presence or absence of vitamin C, and the freshness of the medium may impact biomaterial regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Harshavardhan Mohan, Natesan Karthi, Pavithra Muthukumar Sathya, Vaikundamoorthy Ramalingam, Srivalli Thimmarayan, Mohammad Amjad Hossain, Adithan Aravinthan, Taeho Shin
Summary: This study synthesized (Zn, Ni)-ferrite nanoparticles using a chemical co-precipitation method and evaluated their cytotoxicity and osteogenic properties. The results showed that these nanoparticles had low toxicity and could potentially be used as biomaterials in regenerative medicine.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2022)
Article
Cell Biology
Zhengtao Gu, Denghui Xie, Rui Ding, Caiqiang Huang, Yiyan Qiu
Summary: The study shows that GPR173 signaling is involved in regulating osteoblastic differentiation in MC3T3-E1 cells, with its agonist phoenixin 20 promoting the expression of osteoblast signature genes and activities. The induction by phoenixin 20 relies on the key regulator Runx2 and the activation of MAPK kinase p38. Silencing of GPR173 attenuates phoenixin 20-mediated osteoblastic differentiation, indicating its dependence on the receptor.
Article
Biochemistry & Molecular Biology
Sagrario Martin-Aragon, Paloma Bermejo-Bescos, Juana Benedi, Carlos Raposo, Franklim Marques, Eirini K. Kydonaki, Paraskevi Gkiata, Yiannis Koutedakis, Georgia Ntina, Andres E. Carrillo, Tania Amorim
Summary: The study showed that bovine colostrum can reduce glucocorticoid-induced apoptosis in osteoblastic cells, possibly through the activation of the ERK pathway and modulation of the Hsp70 system. This provides preliminary evidence on how bovine colostrum, as a complex and multi-component dairy product, may affect osteoblastic cell survival undergoing apoptosis, in addition to its neuroprotective action.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yixin Mao, Yang Chen, Wenjin Cai, Wanying Jiang, Xiaoyu Sun, Jun Zeng, Hongning Wang, Xia Wang, Wenmei Dong, Jianfeng Ma, Richard T. Jaspers, Shengbin Huang, Gang Wu
Summary: Titanium ions stimulate osteoblast apoptosis and have a negative effect on implant failure. Mitochondrial abnormalities are related to osteoblast dysfunction. This study found that mitochondrial oxidative stress and dysfunction play a role in titanium ion-induced apoptosis of osteoblastic cells. Mitochondrial-targeted antioxidants can alleviate the cytotoxic effects of titanium ions on osteoblasts. The primary regulator of mitochondrial permeability transition pore, cyclophilin D, is involved in mitochondrial dysfunction and osteoblast cell apoptosis induced by titanium ions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Lu Bian, YiQing Wu, Jiawei Wu, Peng Zhao, Xijiang Zhao
Summary: The study used nasal mucosal-derived EMSCs to promote osteogenic differentiation in MC3T3-E1 cells and found that EMSC-derived Shh played a role in enhancing osteogenic differentiation in MC3T3-E1 cells.
MOLECULAR BIOLOGY REPORTS
(2023)