Article
Developmental Biology
Masaki Kinoshita, Toshihiro Kobayashi, Benjamin Planells, Doris Klisch, Daniel Spindlow, Hideki Masaki, Susanne Bornelov, Giuliano Giuseppe Stirparo, Hitomi Matsunari, Ayuko Uchikura, Ismael Lamas-Toranzo, Jennifer Nichols, Hiromitsu Nakauchi, Hiroshi Nagashima, Ramiro Alberio, Austin Smith
Summary: This study successfully established pluripotent stem cell lines from porcine, ovine, and bovine embryos without using feeder cells or serum factors. The cells showed differentiation in teratomas and in vitro, with global transcriptome analyses revealing commonality in transcription factor expression across the three species. The research also demonstrated the generation of cloned porcine fetuses using genetically modified AFX stem cells through nuclear transfer.
Article
Oncology
Gulab Sher, Tariq Masoodi, Kalyani Patil, Sabah Akhtar, Shilpa Kuttikrishnan, Aamir Ahmad, Shahab Uddin
Summary: Since the introduction of the cancer stem cell paradigm, significant progress has been made in understanding the role of cancer stem cells (CSCs) in tumor biology, including metastasis and drug resistance. The transcription factor FOXM1 has emerged as a key regulator of CSCs, involved in their formation, maintenance, and functionality. This review summarizes the evidence for the mechanisms by which FOXM1 promotes stemness in different types of cancers and highlights its prognostic importance.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Vijay V. Vishnu, Bh. Muralikrishna, Archana Verma, Sanjeev Chavan Nayak, Divya Tej Sowpati, Vegesna Radha, P. Chandra Shekar
Summary: C3G plays a crucial role in regulating pluripotency and differentiation of mouse embryonic stem cells by influencing the expression of self-renewal factors, activity of signaling pathways, and cell adhesion. Its absence results in enhanced clonogenicity, blocked differentiation, dysregulated gene expression, and decreased adhesion capability in mouse ES cells.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ana Virginia Sanchez-Sanchez, Antonio Garcia-Espana, Pilar Sanchez-Gomez, Jaime Font-de-Mora, Marian Merino, Jose Luis Mullor
Summary: NANOG is a key transcription factor for maintaining pluripotency, with elevated expression levels in various human cancers and a role in tumor metastasis. Studies show that NANOG regulates cancer cell migration through the SDF1/CXCR4 pathway, suggesting a potential therapeutic target for Nanog-dependent tumor progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yong-Jin Kim, Amin Tamadon, Yoon-Young Kim, Byeong-Cheol Kang, Seung-Yup Ku
Summary: This review focuses on the current knowledge of epigenetic regulation of cardiomyocyte proliferation and differentiation from embryonic and induced pluripotent stem cells through histone modification and microRNAs, the maintenance of pluripotency, and its alteration during cardiac lineage differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Shuang Zhao, Chuanyu Zhang, Jia Xu, Siying Liu, Lu Yu, Shang Chen, Hang Wen, Zongjin Li, Na Liu
Summary: This study identified potential interacting proteins with Dppa3 and found that Dppa3 safeguards the stability of Uhrf1 and Nanog by inhibiting proteasome-associated degradation in ES cells.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Julian O. Kimura, Marcela Bolanos, Lorenzo Ricci, Mansi Srivastava
Summary: This study reveals the cellular mechanism and molecular trajectory for the formation of adult pluripotent stem cells (aPSCs) during embryonic development. By utilizing lineage tracing and single-cell transcriptome profiling, the researchers identified a molecular trajectory for neoblast formation and showed that aPSCs derive from one lineage instead of multiple tissue-specific lineages.
Article
Oncology
Xin Lyu, Lizhong Zeng, Jie Shi, Zongjuan Ming, Wei Li, Boxuan Liu, Yang Chen, Bo Yuan, Ruiying Sun, Jingyan Yuan, Nannan Zhao, Xia Yang, Guoan Chen, Shuanying Yang
Summary: The resistance to icotinib treatment is associated with the autophagy mechanism induced by the STAT3/FOXM1/ATG7 signaling, providing new insights into the potential clinical value of ATG7-dependent autophagy in icotinib treatment.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Daniel Olivieri, Eleonora Castelli, Yumiko K. Kawamura, Panagiotis Papasaikas, Ilya Lukonin, Melanie Rittirsch, Daniel Hess, Sebastien A. Smallwood, Michael B. Stadler, Antoine H. F. M. Peters, Joerg Betschinger
Summary: Mouse embryonic stem cells are biased towards producing embryonic endoderm rather than extraembryonic endoderm, which is regulated by transcriptional repressors Hdac3 and Dax1. Pluripotency factors Nr5a2 and Esrrb promote cell type conversion, and perturbation of the barrier extends mESC potency. This study shows that transcriptional repressors stabilize pluripotency by biasing the equilibrium between embryonic and extraembryonic lineages in mESCs.
Article
Genetics & Heredity
Riccardo M. Betto, Linda Diamante, Valentina Perrera, Matteo Audano, Stefania Rapelli, Andrea Lauria, Danny Incarnato, Mattia Arboit, Silvia Pedretti, Giovanni Rigoni, Vincent Guerineau, David Touboul, Giuliano Giuseppe Stirparo, Tim Lohoff, Thorsten Boroviak, Paolo Grumati, Maria E. Soriano, Jennifer Nichols, Nico Mitro, Salvatore Oliviero, Graziano Martello
Summary: This study reveals that LIF-Stat3 signaling induces genomic hypomethylation through metabolic reconfiguration, impacting the developmental plasticity of cells. Genome methylation is dynamically controlled via regulation of alpha-ketoglutarate availability or Stat3 activation. The transcription factor Otx2 is linked to DNA methylation through alpha-ketoglutarate, affecting the epigenome.
Article
Cell Biology
Elena Carbognin, Valentina Carlini, Francesco Panariello, Martina Chieregato, Elena Guerzoni, Davide Benvegnu, Valentina Perrera, Cristina Malucelli, Marcella Cesana, Antonio Grimaldi, Margherita Mutarelli, Annamaria Carissimo, Eitan Tannenbaum, Hillel Kugler, Jamie A. Hackett, Davide Cacchiarelli, Graziano Martello
Summary: Carbognin et al. found that ESRRB is essential for the activation of formative genes during the transition of naive embryonic stem cells to a formative state. Inactivation of Esrrb leads to abnormal expression of specific markers and impairs the ability of the cells to self-organize. ESRRB occupies key formative genes in both naive cells and throughout the formative state, kickstarting the formative transition and enabling unbiased multi-lineage differentiation.
NATURE CELL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell & Tissue Engineering
Kevin Hayes, Yun-Kyo Kim, Martin F. Pera
Summary: Nodal is a crucial factor in mammalian embryonic development and the maintenance of pluripotent stem cells. Its signaling pathway may play an important role in regulating self-renewal in hPSC.