Article
Agriculture, Dairy & Animal Science
Ahmed Magdy Selim, Samar Magdy Atwa, Attia Abdullah El Gedawy, Emad Elsayed Younis
Summary: This study conducted the first phylogenetic analysis of C. pseudotuberculosis isolate in Egypt, showing high homology between the PLD gene isolate and strains from India, Sudan, etc. The findings provide valuable information on the molecular detection and phylogeny of C. pseudotuberculosis in Egypt, and could be beneficial for other CLA endemic countries with similar animal breeding practices.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Microbiology
Ramon Mendes dos Santos, Silvana Beutinger Marchioro, Marcos Borges Ribeiro, Maria da Conceicao Aquino de Sa, Vitor Cordeiro Pereira, Antonio Pedro Froes de Farias, Roberto Jose Meyer Nascimento, Songeli Menezes Freire
Summary: This study evaluated the antigenic potential of four C. pseudotuberculosis recombinant proteins in serum samples of experimentally infected goats and sheep, and found that the rSodC protein could recognize antibodies against all evaluated strains, making it a strong candidate for the diagnosis of C. pseudotuberculosis infection.
CURRENT MICROBIOLOGY
(2022)
Article
Microbiology
Jingmei Tan, Wenyi Yi, Zhiying Wang, Chen Ye, Shangquan Tian, Xiaoxia Li, Anlong Zou, Xiawei Zhao, Yongfeng Yuan, Xiaohan Wang, Shijun Hu, Zuoyong Zhou
Summary: TRIM21 plays a crucial role in preventing C. pseudotuberculosis infection by limiting bacterial replication in macrophages and protecting infected cells from death. The absence of TRIM21 results in higher mortality, bacterial load, abscess formation, and organ lesions in C. pseudotuberculosis-infected mice, along with increased secretion of pro-inflammatory cytokines in macrophages, sera, organs, and ascites.
VETERINARY MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Jun Kawase, Tomotake Sakai, Masaaki Iwaki, Kaoru Umeda, Aiko Fukuma, Naoki Fujisawa, Yuta Kawakami, Hiroki Hayashi, Mieko Wada
Summary: A multiplex real-time PCR assay has been developed to rapidly discriminate Corynebacterium ulcerans from Corynebacterium pseudotuberculosis and detect the bacterial diphtheria toxin gene. This assay is a valuable tool for identification of potentially toxigenic C. ulcerans.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Review
Biotechnology & Applied Microbiology
Rodrigo Barros de Pinho, Mara Thais de Oliveira Silva, Francisco Silvestre Brilhante Bezerra, Sibele Borsuk
Summary: Caseous lymphadenitis (CLA) is an infectious chronic disease that causes economic losses in sheep and goat breeding worldwide. Although commercial vaccines are available, none of them provide total protection, hence experimental vaccines are being developed to enhance protective immune response.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Helmy A. A. Torky, Hebatallah M. Saad, Samy A. Khaliel, Asmaa T. Kassih, Jean-Marc Sabatier, Gaber El-Saber Batiha, Helal F. Hetta, Eman M. Elghazaly, Michel De Waard
Summary: Corynebacterium pseudotuberculosis (C. pseudotuberculosis) is a causative agent of chronic diseases in animals and humans, with poor therapeutic response. A study analyzed 120 samples and found only 10% positive for C. pseudotuberculosis. Genetic analysis showed intra-subtyping variation. The study also identified immune expression patterns in affected lymph nodes, indicating the involvement of the NF-kappa B/p65 signaling pathway in the inflammatory response to control the pathogen.
Article
Chemistry, Analytical
Zhang Zhang, Luhao Guan, Juan Yao, Lijia Li, Chunfang Liu, Yongcan Guo, Guoming Xie
Summary: In this study, a closed-tube, dual-labeled RNA-modified probes and RNase H II-assisted real-time LAMP (RART-LAMP) method was proposed for SNP genotyping. This method allows for rapid and accurate nucleic acid analysis in resource-limited settings, and can be applied to various sample types including blood, saliva, and swabs.
ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Guillaume Girault, Ludivine Perrot, Virginie Mick, Claire Ponsart
Summary: In this study, a high-resolution melting (HRM) method was developed for the rapid identification and classification of the Brucella genus based on 17 specific single nucleotide polymorphisms (SNPs) and SNP analysis. This genotyping tool provides a new and quick alternative for Brucella identification, significantly saving time for the diagnosis of brucellosis.
Article
Veterinary Sciences
Zanan M. Taha
Summary: High genetic diversity was observed in C. pseudotuberculosis strains in Duhok, Iraq. ERIC-PCR is a valuable technique for epidemiological studies. Strains of C. pseudotuberculosis from sheep showed genetic diversity compared to those from goats.
EXPLORATORY ANIMAL AND MEDICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Aida Hematzadeh, Masoud Haghkhah
Summary: The study aimed to diagnose and biotype 96 isolates of P. aeruginosa from infections in Shiraz hospitals, revealing a high genetic variation through PCR, ERIC, and RAPD analysis. The high polymorphism of P. aeruginosa isolates was demonstrated based on the results with dice similarity coefficient of greater than 80%.
Article
Microbiology
Bram Spruijtenburg, Cynthea C. S. Z. Baqueiro, Arnaldo L. Colombo, Eelco F. J. Meijer, Joao N. de Almeida, Indira Berrio, Norma B. Fernandez, Guilherme M. Chaves, Jacques F. Meis, Theun de Groot
Summary: Candida tropicalis is a common opportunistic pathogen causing infections in Latin America, and the rise of antifungal-resistant isolates is becoming a concern. This study utilized short tandem repeat (STR) genotyping and antifungal susceptibility testing (AFST) to investigate the population genomics and resistance patterns of 230 C. tropicalis isolates. The results revealed outbreak events, the presence of anidulafungin-resistant isolates, and azole resistance-associated substitutions. The findings highlight the importance of this STR genotyping scheme for identifying outbreaks and understanding the spread of antifungal-resistant C. tropicalis isolates.
Article
Agriculture, Dairy & Animal Science
Rasha Gomaa Tawfik, Mahmoud F. F. Gawish, Mahmoud M. M. Abotaleb, Hassan S. S. Nada, Kareem Morsy, Mohamed M. A. Abumandour, Helmy Torky
Summary: The study aimed to diagnose Salmonella from chickens and calves, determine its resistance to antimicrobials, characterize integrons and beta lactamase genes in MDR Salmonella, and detect genetic relationships between isolates using ERIC PCR. Results showed MDR in Salmonella isolates and the presence of blaTEM and class 1 integron genes. ERIC PCR revealed clonal relationships between isolates, with S. Enteritidis isolates from poultry and calves showing 100% similarity, indicating possible cross-infection between animals on the same farm, while S. Kentucky isolates showed lower serovarities.
Article
Veterinary Sciences
Arife Ezgi Telli, Yusuf Bicer, Nihat Telli, Candan Gungor, Gamze Turkal, Nurhan Ertas Onmaz
Summary: The present study aimed to identify pathogenic Escherichia coli and Salmonella spp. in retail chicken samples and investigate their genetic relation. The results showed a high incidence of E. coli and Salmonella spp. in chicken carcasses, but no virulent strains were detected. The genetic diversity of Salmonella spp. was found to be higher compared to E. coli isolates.
PAKISTAN VETERINARY JOURNAL
(2022)
Article
Genetics & Heredity
Michele Ragazzo, Giulio Puleri, Valeria Errichiello, Laura Manzo, Laura Luzzi, Saverio Potenza, Claudia Strafella, Cristina Peconi, Fabio Nicastro, Valerio Caputo, Emiliano Giardina
Summary: The study evaluated a custom plate of OpenArray (TM) technology for testing 60 SNPs validated for predicting eye color, hair color, skin pigmentation, and personal identification. The technology showed high concordance rates, good sensitivity to different DNA concentrations, and high accuracy in human identification.
Article
Plant Sciences
Abdugaffor Ablazov, Abrar Felemban, Justine Braguy, Hendrik N. J. Kuijer, Salim Al-Babili
Summary: With increasing throughput in both the generation and phenotyping of mutant lines in plants, it is important to have an efficient and reliable genotyping method. We propose an alternative workflow using Phire polymerase and ExoProStar treatment for genotyping mutant plants, which is faster, cheaper, and reduces the risk of contamination and mistakes. The automated sequence analysis systems used in this workflow are accurate and suitable for bulk analysis.
Article
Immunology
Carine Rodrigues Pereira, Thiago de Jesus Sousa, Alessandra Lima da Silva, Roselane Goncalves dos Santos, Silvia Minharro, Dirceia Aparecida Costa Custodio, Derek J. Pickard, David O'Callaghan, Jeffrey T. Foster, Siomar de Castro Soares, Rommel Thiago Juca Ramos, Aristoteles Goes-Neto, Mateus Matiuzzi da Costa, Andrey Pereira Lage, Vasco Azevedo, Elaine Maria Seles Dorneles
Summary: This study reports the first isolation, identification, and characterization of Pseudochrobactrum saccharolyticum (strain 115) from Latin America. The strain was classified as P. saccharolyticum based on the analysis of its whole-genome sequence and 16S rRNA and recA gene sequences. This provides important insights into the diagnosis of bovine brucellosis and highlights the value of including the sequencing of these genes in routine diagnostics.
MICROBES AND INFECTION
(2023)
Article
Peripheral Vascular Disease
Beatriz G. S. Rocha, Caroline C. Picoli, Bryan O. P. Goncalves, Walison N. Silva, Alinne C. Costa, Michele M. Moraes, Pedro A. C. Costa, Gabryella S. P. Santos, Milla R. Almeida, Luciana M. Silva, Youvika Singh, Marcelo Falchetti, Gabriela D. A. Guardia, Pedro P. G. Guimaraes, Remo C. Russo, Rodrigo R. Resende, Mauro C. X. Pinto, Jaime H. Amorim, Vasco A. C. Azevedo, Alexandre Kanashiro, Helder I. Nakaya, Edroaldo L. Rocha, Pedro A. F. Galante, Akiva Mintz, Paul S. Frenette, Alexander Birbrair
Summary: This study reveals the presence of perivascular GFAP+/Plp1+ cells derived from tissue-resident Schwann cells in the tumor microenvironment. Genetic ablation of Schwann cells slows down tumor growth and angiogenesis, while boosting immune surveillance by increasing tumor-infiltrating anti-tumor lymphocytes and reducing immune-suppressor cells. In humans, increased expression of Schwann cell-related genes within melanoma is associated with improved survival.
Article
Cell Biology
Kenneth Lundstrom, Altijana Hromic-Jahjefendic, Esma Bilajac, Alaa A. A. Aljabali, Katarina Baralic, Nagwa A. Sabri, Eslam M. Shehata, Mohamed Raslan, Ana Claudia B. H. Ferreira, Lidiane Orlandi, Angel Serrano-Aroca, Murtaza M. Tambuwala, Vladimir N. Uversky, Vasco Azevedo, Khalid J. Alzahrani, Khalaf F. Alsharifm, Ibrahim F. Halawani, Fuad M. Alzahrani, Elrashdy M. Redwan, Debmalya Barhl
Summary: This review summarizes major events of SARS-CoV-2 infection route, immune response following host-cell infection as an important factor for disease outcome, as well as comorbidity issues of various tissues and organs arising due to COVID-19. Investigations on alterations of host-cell machinery and viral interactions with multiple intracellular signaling pathways could represent a major factor in more effective disease management.
CELLULAR SIGNALLING
(2023)
Article
Cell Biology
Debmalya Barh, Sandeep Tiwari, Lucas Gabriel Rodrigues Gomes, Cecilia Horta Ramalho Pinto, Bruno Silva Andrade, Shaban Ahmad, Alaa A. A. Aljabali, Khalid J. Alzahrani, Hamsa Jameel Banjer, Sk Sarif Hassan, Elrashdy M. Redwan, Khalid Raza, Aristoteles Goes-Neto, Robinson Sabino-Silva, Kenneth Lundstrom, Vladimir N. Uversky, Vasco Azevedo, Murtaza M. Tambuwala
Summary: The Omicron variant of SARS-CoV-2 is highly transmissible with decreased disease severity. It has lower pathogenicity but higher antigenicity compared to other variants. These findings contribute to our understanding of variant dynamics and can help in managing emerging variants.
Article
Microbiology
Pedro Negri Bernardino, Carolina Lechinski de Paula, Ana Flavia Marques Pereira, Marcio Garcia Ribeiro, Vasco Ariston de Carvalho Azevedo, Alexandre Secorun Borges, Ary Fernandes Jr, Jose Paes Oliveira-Filho
Summary: Caseous lymphadenitis is a disease caused by Corynebacterium pseudotuberculosis with minimal zoonotic potential. This study investigated the antimicrobial action of 2',3'-dideoxiadenosine (ddATP) against C. pseudotuberculosis and its potential synergy with penicillin, suggesting the potential of nucleotide analogs and combination therapy as a novel treatment for C. pseudotuberculosis infections.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Microbiology
Marcio Garcia Ribeiro, Thiago Tourinho Pereira, Patrik Junior de Lima Paz, Beatriz Oliveira de Almeida, Carmen S. Araujo Cervino, Carolina Aparecida Rodrigues, Gabrielly Terra Sartori Santos, Larissa Maria de Souza Freire, Fabio Vinicius Ramos Portilho, Marcelo Fagali Arabe Filho, Natalia Rodrigues Paschoal, Thais Spessotto Bello, Jane Megid, Helio Langoni, Camila Michele Appolinario, Alexandre Secorun Borges, Rogerio Martins Amorim, Rogerio Giuffrida, Jose Paes de Oliveira Filho, Amanda Keller Siqueira, Fernando Jose Paganini Listoni, Antonio Carlos Paes
Summary: Central nervous system (CNS) infections are serious clinical conditions in domestic species, often leading to severe consequences, disabilities, or high fatality rates. In this retrospective study, we investigated 136 neurologic cases in domestic species from 2005 to 2021 and found that 25% of cerebrospinal fluid samples contained bacterial isolates. The most common bacterial pathogens were Staphylococcus aureus, Escherichia coli, Staphylococcus beta-hemolytic, and Trueperella pyogenes. These isolates exhibited varying levels of susceptibility and resistance to different antimicrobials, and a high lethality rate was observed in animals with bacterial isolation.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Ecology
Priscyla dos Santos Ribeiro, Natalia Barbosa Carvalho, Flavia Aburjaile, Thiago Sousa, Graciete Verissimo, Talita Gomes, Fabio Neves, Luiza Blanco, Joao Antonio Lima, Daiana de Oliveira, Arun Kumar Jaiswal, Bertram Brenig, Siomar Soares, Rommel Ramos, Mateus Matiuzzi, Aristoteles Goes-Neto, Claudio Pereira Figueira, Federico Costa, Paula Ristow, Vasco Azevedo
Summary: This study identified a new non-pathogenic species of spirochetes in biofilms in a polluted urban environment, suggesting that biofilms may serve as natural reservoirs for leptospires.
Review
Biochemistry & Molecular Biology
Emilio Mateev, Maya Georgieva, Alexandrina Mateeva, Alexander Zlatkov, Shaban Ahmad, Khalid Raza, Vasco Azevedo, Debmalya Barh
Summary: With the increase in neurodegenerative diseases, monoamine oxidase type B (MAO-B) inhibitors are emerging as promising structures for treatment. Structure-based virtual screening (SBVS), specifically using molecular docking, is a useful tool for drug discovery and development. This study provides insight into the role of MAOs in treating neurodegenerative diseases, discusses the advantages and disadvantages of docking simulations and software, and explores the active sites of MAO-A and MAO-B. New chemical classes of MAO-B inhibitors and their essential fragments are also discussed, providing potential directions for further research.
Article
Immunology
Muhammad Usman, Aaima Ayub, Sabahat Habib, Muhammad Suleman Rana, Zaira Rehman, Ali Zohaib, Syed Babar Jamal, Arun Kumar Jaiswal, Bruno Silva Andrade, Vasco de Carvalho Azevedo, Muhammad Faheem, Aneela Javed
Summary: In this study, a multivalent vaccine was designed using VP4 and VP7 proteins of Rotavirus A. Epitopes with antigenicity, non-allergenicity, homology with humans, and anti-inflammatory properties were screened to construct the vaccine. Immune simulation showed promising results in both Asia and worldwide. However, in-vitro/in-vivo screening is necessary for a definitive conclusion.
Article
Microbiology
Joyce Dutra, Glen Garcia, Rosimeire Gomes, Mariana Cardoso, Arley Cortes, Tales Silva, Luis de Jesus, Luciano Rodrigues, Andria Freitas, Vinicius Waldow, Juliana Laguna, Gabriela Campos, Monique Americo, Rubens Akamine, Maira de Sousa, Claudia Groposo, Henrique Figueiredo, Vasco Azevedo, Aristoteles Goes-Neto
Summary: The study aimed to analyze the physicochemical features of microbial communities occurring in produced water and enrichment cultures in oil pipelines. It was found that produced water samples have higher phylogenetic diversity of bacteria and archaea, while enrichment cultures have higher dominance of bacterial genera associated with microbial influenced corrosion (MIC). All samples had a core community composed of 19 distinct genera, with Desulfovibrio as the dominant genus. Metabarcoding of uncultured produced water samples, along with physicochemical characterization, was suggested as a more efficient method for monitoring MIC in oil industry facilities.
Article
Microbiology
Juan Carlos Ariute, Andrei Giachetto Felice, Siomar Soares, Marco Aurelio Siqueira da Gama, Elineide Barbosa de Souza, Vasco Azevedo, Bertram Brenig, Flavia Aburjaile, Ana Maria Benko-Iseppon
Summary: In this study, 14 Ralstonia solanacearum isolates from Brazil were sequenced and analyzed, revealing their similarity to R. solanacearum genomes in NCBI. The new isolates shared more Rips with Moko IIA and Moko IIB than with other public BW genome isolates from Brazil. These findings provide important insights into the association between Rips repertoire and host specificities.
Article
Infectious Diseases
Jordana Almeida Santana, Amanda Oliveira Paraguassu, Ranielle Stephanie Toledo Santana, Rafael Gariglio Clark Xavier, Patricia Maria Colleto Freitas, Flavia Figueira Aburjaile, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Anders Miki Bojesen, Rodrigo Otavio Silveira Silva
Summary: This study evaluated the risk factors associated with MRSP colonization in dogs admitted to an ICU, and reported antimicrobial resistance profiles and genetic relatedness of MRSP isolates. The results found that female sex, age (>7 years), and previous antimicrobial treatment were associated with MRSP acquisition. Whole-genome sequencing revealed the presence of the same MRSP clone causing infections in dogs outside the ICU. Therefore, improved infection prevention and control practices are necessary in veterinary hospitals, especially in ICUs.
Article
Genetics & Heredity
Carine Rodrigues Pereira, Rodrigo Bentes Kato, Fabricio Almeida Araujo, Alessandra Lima da Silva, Roselane Goncalves dos Santos, Thiago de Jesus Sousa, Raquel Costa Neia, Saulo Britto da Silva, Charles H. D. Williamson, John Gillece, Andrey Pereira Lage, David O'Callaghan, Derek Pickard, Rommel Thiago Juca Ramos, Vasco Ariston de Carvalho Azevedo, Jeffrey T. Foster, Elaine Maria Seles Dorneles
Summary: This study investigated the genetic determinants responsible for antimicrobial resistance in 53 genomes of B. abortus strains isolated from cattle in Brazil. Eight genes associated with antimicrobial resistance were identified, but no association was found between the genetic polymorphisms in these genes and the observed resistance phenotypes in the Brazilian strains.
Article
Immunology
Maisa Santos Fonseca, Kathleen Ramos Deegan, Luiz Marcelo Tome, Marcos Antonio Mendonca, Ana Rita Sokolonski, Leane Queiroz Gondim, Vasco Azevedo, Roberto Meyer, Ljubica Tasic, Aristoteles Goes-Neto, Ricardo Wagner Portela
Summary: This study examines a novel pathogen, Candida haemulonii, causing cutaneous infection in a Boa constrictor snake. The fungus was identified and its drug sensitivity was tested, revealing strong inhibition for all drugs except fluconazole and itraconazole. A biogenic silver nanoparticle-based ointment successfully treated the clinical symptoms in the snake. The presence of these infections in snakes near human habitats highlights the importance of wildlife health monitoring for emerging and opportunistic diseases in peri-urban environments.
MICROBIAL PATHOGENESIS
(2023)
Article
Agriculture, Multidisciplinary
Carolina Menezes Suassuna de Souza, Maiara Garcia Blagitz, Fernando Nogueira Souza, Camila Freitas Batista, Daniel Magalhaes Lima, Alexandre Jose Alves, Marcos Bryan Heinemann, Eduardo Milton Ramos Sanchez, Hugo Frias Torres, Artur Cezar de Carvalho Fernandes, Alice Maria Melville Paiva Della Libera
Summary: This study investigated the predictive values of milk leukocyte differentials (MLD) for improving the diagnosis of intramammary infections (IMIs) and subclinical mastitis. The results showed that the combination of T CD8+ lymphocytes with PMNLs and macrophages can better diagnose bovine IMIs.
