Article
Computer Science, Interdisciplinary Applications
Sayan Roychowdhury, Erik W. Draeger, Amanda Randles
Summary: With the increase in computational power, it is important to consider the spatial distributions of cells in simulations. Multiple configurations need to be sampled to assess the potential cell arrangements. Metrics such as the radial distribution function and Jaccard index are proposed to identify structure and quantify similarity between cell configurations. The study expands upon previous work, increasing the data set size and comparing the effects of different particle shapes.
JOURNAL OF COMPUTATIONAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Viktoriia Oleksa, Iveta Bernatova, Vitalii Patsula, Silvia Liskova, Peter Balis, Jana Radosinska, Andrea Micurova, Michal Kluknavsky, Tomas Jasenovec, Dominika Radosinska, Hana Mackova, Daniel Horak
Summary: The acute intravenous administration of Fe3O4@PEG-Ale did not produce negative effects on blood pressure regulation, vascular function, and red blood cells in hypertensive rats, according to the study results.
Article
Multidisciplinary Sciences
Saman Ebrahimi, Prosenjit Bagchi
Summary: This study investigates the feasibility of using machine learning techniques to predict blood flow and red blood cell (RBC) distributions in physiologically realistic vascular networks. By acquiring data from high-fidelity simulations, machine learning models can accurately predict the changes in blood flow, RBC distributions, and hemodynamics at different vascular levels and time scales.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Article
Ecology
Gregory F. Albery, Tim H. Clutton-Brock, Alison Morris, Sean Morris, Josephine M. Pemberton, Daniel H. Nussey, Josh A. Firth
Summary: The article describes the decline in social connectedness in individual European red deer as they age, and identifies the underlying mechanisms through spatial and social network analyses. These findings contribute to our understanding of the ecological and evolutionary processes in wild animal populations.
NATURE ECOLOGY & EVOLUTION
(2022)
Article
Microbiology
Zi-Han Wei, Lin Xie, Yong-Jing Wang, Jiang-Xing Zhuang, Jian-Jun Niu, Li-Li Liu
Summary: RBC lysis pretreatment can significantly improve the yield of T. pallidum DNA from whole blood. A qPCR assay targeting the polA gene was developed and showed good linearity and high amplification efficiency. Whole blood/lysed RBCs were found to be the ideal sample for acquiring blood T. pallidum DNA.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biology
Elisaveta Skverchinskaya, Nadezhda Levdarovich, Alexander Ivanov, Igor Mindukshev, Anton Bukatin
Summary: An important issue in cancer chemotherapy is reducing side effects, such as anemia caused by the toxicity of chemotherapy drugs. This study found that chemotherapy drugs have a noticeable toxic effect on red blood cells (RBCs), but the effect is lower than drugs that disrupt cell division. By simulating RBCs' transport in microchannels, researchers assessed the cells' functionality and the drugs' therapeutic doses, which could be used to minimize anemia side effects during cancer chemotherapy.
Article
Chemistry, Analytical
Ningbo Li, Minshu Du, Yao Meng, Huidan Xue, Fahe Cao, Hsien-Yi Hsu, Feng Liu
Summary: This research introduces a novel approach for quantitative and reliable detection of red blood cell agglutination using collision electrochemical technique. It provides insights on the concentration, size, and agglutination status of red blood cells, and shows potential applications in blood grouping sensors and immunoassays for infectious diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Biotechnology & Applied Microbiology
Mengran Chen, Yamei Leng, Chuan He, Xuefeng Li, Lei Zhao, Ying Qu, Yu Wu
Summary: Red blood cells (RBCs) are abundant cells in the body with unique biological and physical properties. Due to their low immunogenicity, long-circulating cycle, and immune characteristics, RBCs have shown great potential as delivery vehicles. This article compares the physiological and physical characteristics of RBCs, analyzes their advantages in delivery systems, and summarizes their applications in biomedicine.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Plant Sciences
Siming Wang, Yuchu Zhao, Junjie Yang, Shichao Liu, Weifeng Ni, Xueyuan Bai, Ze Yang, Daqing Zhao, Meichen Liu
Summary: This study aimed to investigate the protective effect of GPS on oxidatively damaged RBCs and explore the molecular mechanisms from the perspective of glycolysis and gluconeogenesis pathways. The results showed that GPS effectively protected RBCs from oxidative stress damage, promoted glycolysis, and regulated antioxidant defense system and energy metabolism. GPS achieved these effects by activating the PGC-1 alpha pathway and inhibiting gluconeogenesis.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Review
Physiology
Gregory Barshtein, Ivana Pajic-Lijakovic, Alexander Gural
Summary: Red blood cells' deformability plays a crucial role in adapting to flow conditions, with high deformability facilitating flow through small blood vessels. Loss of deformability may lead to cell rigidity and dysfunction.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Brianna M. Vickerman, Colin P. O'Banion, Xianming Tan, David S. Lawrence
Summary: Protein therapeutics have high selectivity and potency, but are often limited by short half-lives and off-target effects. This study introduces a targeted protein delivery system using engineered red blood cells and light-triggered hemolysis for drug release. The strategy shows spatiotemporal control and successful delivery of therapeutic proteins in vitro and in vivo.
ACS CENTRAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Giada Bianchetti, Gaetano Emanuele Rizzo, Cassandra Serantoni, Alessio Abeltino, Alessandro Rizzi, Linda Tartaglione, Salvatore Caputo, Andrea Flex, Marco De Spirito, Dario Pitocco, Giuseppe Maulucci
Summary: This study investigates the importance of red blood cell membrane fluidity in monitoring macroangiopathic complications related to type 2 diabetes. The study found that patients with Peripheral Artery Disease (PAD) have significantly increased red blood cell membrane fluidity, which is associated with spatial reconfiguration of LC domains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sumit Nanda, Shatabdi Bhattacharjee, Daniel N. Cox, Giorgio A. Ascoli
Summary: Dendritic morphology plays a crucial role in neuronal signal processing, and can be described by dendrogram topology and spatial embedding. This study examines the relationship between the distribution of microtubules and actin filaments and dendritic spatial embedding. The findings suggest that the local distribution of cytoskeletal components can fully determine the overall geometry of dendritic arbors. A computational model is developed to demonstrate the adequacy of cytoskeletal distributions in reproducing different dendritic architectures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Shreya S. Deshmukh, Bikash Shakya, Anna Chen, Naside Gozde Durmus, Bryan Greenhouse, Elizabeth S. Egan, Utkan Demirci
Summary: The new method uses changes in cell density and magnetic susceptibility to separate and analyze different cell types, especially infected cells. By balancing gravitational and magnetic forces, it can effectively distinguish infected cells at different biological stages, making multidimensional biophysical measurements possible.
COMMUNICATIONS BIOLOGY
(2021)
Article
Surgery
Kasiemobi E. Pulliam, Bernadin Joseph, Amy T. Makley, Charles C. Caldwell, Alex B. Lentsch, Michael D. Goodman, Timothy A. Pritts
Summary: The study showed that washing packed red blood cells during storage can decrease the development of red blood cell storage lesions, reducing microvesicle accumulation and cell-free hemoglobin, with no impact on red blood cell viability.
Article
Biophysics
Joseph van Batenburg-Sherwood, Stavroula Balabani
Summary: Modelling blood flow in microvascular networks is challenging due to haemorheology complexity. Continuum numerical modelling framework can efficiently reproduce measured velocity with optimised rheological parameters, highlighting the importance of asymmetry in RBC velocity and concentration profiles.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Gaia Franzetti, Mirko Bonfanti, Cyrus Tanade, Chung Sim Lim, Janice Tsui, George Hamilton, Vanessa Diaz-Zuccarini, Stavroula Balabani
Summary: This study aimed to develop a computational framework to simulate the hemodynamics of peripheral arteriovenous malformations (pAVMs) and aid in clinical decision making. The results showed that the computational model successfully simulated changes in PAVMs during interventions, providing a foundation for a treatment-planning framework for AVM embolisation.
CARDIOVASCULAR ENGINEERING AND TECHNOLOGY
(2022)
Article
Biophysics
Gaia Franzetti, Mirko Bonfanti, Shervanthi Homer-Vanniasinkam, Vanessa Diaz-Zuccarini, Stavroula Balabani
Summary: This study presents the first experimental study of a patient-specific case of Aortic Dissection (AD) using an anatomically correct phantom combined with a state-of-the-art in vitro platform. The results accurately reproduced personalized AD hemodynamic conditions, providing clinically relevant data that can be used to evaluate surgical procedures, test medical devices, and validate numerical models.
JOURNAL OF BIOMECHANICS
(2022)
Article
Mechanics
D. Pasias, L. Koutsokeras, A. Passos, G. Constantinides, S. Balabani, E. Kaliviotis
Summary: Understanding the influence of the biomechanical properties of blood on surface tension-driven microfluidic flows is crucial for the design of low-cost blood diagnostic devices. In this study, the effects of specific hemorheological factors on flows in a superhydrophilic microchannel were examined using experimental techniques. It was found that the viscosity of the sample and the deformability of erythrocytes influenced the mean velocity and velocity profiles of the blood flow.
Article
Immunology
Oleg Yarishkin, Tam T. T. Phuong, Felix Vazquez-Chona, Jacques Bertrand, Joseph van Battenburg-Sherwood, Sarah N. Redmon, Christopher N. Rudzitis, Monika Lakk, Jackson M. Baumann, Marc Freichel, Eun-Mi Hwang, Darryl Overby, David Krizaj
Summary: The trabecular meshwork (TM) cells in the eyes actively regulate intraocular pressure by employing mechanotransduction and calcium signaling. The activation of TRPV4 channels stimulates TRPM4 channels, leading to dynamic calcium signaling in TM cells. This finding provides insights into the importance of pressure regulation in the eyes and potential therapeutic approaches.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Ophthalmology
Michael Madekurozwa, Ester Reina-Torres, Darryl R. Overby, Joseph van Batenburg-Sherwood
Summary: Elevation of intraocular pressure (IOP) is the key risk factor for glaucoma, and reducing IOP is the only effective therapeutic approach. Measuring outflow facility is important for understanding the pathophysiology of glaucoma and testing treatments for lowering IOP. Mouse eyes are commonly used as a model for human aqueous humour dynamics. The ex vivo preparation and postmortem in situ model have different advantages in studying glaucoma. This study investigates the postmortem in situ model and its characteristics compared to the ex vivo model.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Hematology
Andreas Passos, Marinos Louka, Charalambos Vryonidis, Antonis Inglezakis, Constantinos Loizou, Elena Nikiphorou, Savvas Psarelis, Efstathios Kaliviotis
Summary: The erythrocyte sedimentation rate (ESR) test is widely used in clinical practice. A new low cost micro-ESR technique has been developed, which reduces measurement time and blood sample volume. The evaluation shows a high correlation with commercial instruments, indicating its feasibility.
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
(2022)
Review
Multidisciplinary Sciences
T. Boulafentis, T. Lacassagne, N. Cagney, S. Balabani
Summary: Since Taylor's seminal work in 1923, Taylor-Couette flow has been widely studied to understand hydrodynamic instabilities and bifurcation phenomena. In recent years, there has been growing interest in studying TC flows with complex, viscoelastic fluids. The transitions to elastic turbulence or elasto-inertial turbulence have revealed fascinating dynamics and flow states. However, the nature of these transitions and the relationship between elastic turbulence and elasto-inertial turbulence are not fully understood.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jeffrey O'Callaghan, Conor Delaney, Merissa O'Connor, Joseph van Batenburg-Sherwood, Martin Schicht, Elke Luetjen-Drecoll, Natalie Hudson, Sorcha Ni Dhubhghaill, Peter Humphries, Chris Stanley, Annahita Keravala, Thomas Chalberg, Matthew S. Lawrence, Matthew Campbell
Summary: Approximately 80 million people globally are affected by glaucoma, with a projected increase to over 110 million by 2040. Patient compliance issues and treatment resistance pose risks of permanent vision loss. Gene therapy-based methods, specifically AAV9-mediated expression of MMP-3, show promise in increasing outflow and can be safely tested in clinical trials.
Article
Engineering, Chemical
Mengqi Li, Theofilos Boulafentis, Antonios Stathoulopoulos, Zhaomiao Liu, Stavroula Balabani
Summary: The role of elasticity on the flow topology inside viscoelastic (Boger) droplets moving in a rectangular micro-channel is experimentally examined using micro Particle Image Velocimetry (μPIV). The results show that varying the Wi number alters the flow topology inside the droplets, and when the Wi and elastic number increase, a new flow structure is observed characterized by two recirculating regions. These findings highlight the importance of elasticity in microdroplet flows and the potential of tuning elasticity for microfluidic applications.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Biophysics
Chotirawee Chatpattanasiri, Gaia Franzetti, Mirko Bonfanti, Vanessa Diaz-Zuccarini, Stavroula Balabani
Summary: This study focuses on the use of reduced order models (ROMs) to reconstruct velocity fields in a patient-specific dissected aorta, comparing ROMs obtained from robust and traditional proper orthogonal decomposition (POD). Results show that robust POD enhances the quality of the data and fewer modes are needed to capture the kinetic energy of the flow. The study also finds that the coherent structures of the aortic dissection flow can be described by a small number of POD modes, simplifying the problem.
JOURNAL OF BIOMECHANICS
(2023)
Article
Chemistry, Analytical
Melissa Hexter, Joseph van Batenburg-Sherwood, Parastoo Hashemi
Summary: This study addresses the common issues of traditional FIA cells in FSCV by creating a robust plug-and-play flow cell, improving the accuracy and stability of FSCV calibration.
ACS MEASUREMENT SCIENCE AU
(2023)
Article
Ophthalmology
Colleen M. McDowell, Krishnakumar Kizhatil, Michael H. Elliott, Darryl R. Overby, Joseph Van Batenburg-Sherwood, J. Cameron Millar, Markus H. Kuehn, Gulab Zode, Ted S. Acott, Michael G. Anderson, Sanjoy K. Bhattacharya, Jacques A. Bertrand, Terete Borras, Diane E. Bovenkamp, Lin Cheng, John Danias, Michael Lucio De Ieso, Yiqin Du, Jennifer A. Faralli, Rudolf Fuchshofer, Preethi S. Ganapathy, Haiyan Gong, Samuel Herberg, Humberto Hernandez, Peter Humphries, Simon W. M. John, Paul L. Kaufman, Kate E. Keller, Mary J. Kelley, Ruth A. Kelly, David Krizaj, Ajay Kumar, Brian C. Leonard, Raquel L. Lieberman, Paloma Liton, Yutao Liu, Katy C. Liu, Navita N. Lopez, Weiming Mao, Timur Mavlyutov, Fiona McDonnell, Gillian J. McLellan, Philip Mzyk, Andrews Nartey, Louis R. Pasquale, Gaurang C. Patel, Padmanabhan P. Pattabiraman, Donna M. Peters, Vijaykrishna Raghunathan, Ponugoti Vasantha Rao, Naga Rayana, Urmimala Raychaudhuri, Ester Reina-Torres, Ruiyi Ren, Douglas Rhee, Uttio Roy Chowdhury, John R. Samples, E. Griffen Samples, Najam Sharif, Joel S. Schuman, Val C. Sheffield, Cooper H. Stevenson, Avinash Soundararajan, Preeti Subramanian, Chenna Kesavulu Sugali, Yang Sun, Carol B. Toris, Karen Y. Torrejon, Amir Vahabikashi, Janice A. Vranka, Ting Wang, Colin E. Willoughby, Chen Xin, Hongmin Yun, Hao F. Zhang, Michael P. Fautsch, Ernst R. Tamm, Abbot F. Clark, C. Ross Ethier, W. Daniel Stamer
Summary: This article highlights the importance of using mice as a valuable model system for studying conventional outflow resistance and intraocular pressure. It also outlines the minimum acceptable standards for developing, characterizing, and utilizing mouse models of open-angle ocular hypertension. These standards are expected to enhance scientific rigor and replicability of research findings.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Ophthalmology
Jessica Jasien, A. Thomas Read, Joseph Van Batenburg-Sherwood, Kristin M. Perkumas, C. Ross Ethier, W. Daniel Stamer, Brian C. Samuels
Summary: The tree shrew, with its human-like optic nerve head anatomy and stable aqueous outflow facility, serves as a valuable animal model for glaucoma and pharmacology research, providing advantages over current rodent and nonhuman primate models used in translational research.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)