Article
Genetics & Heredity
Prachi Sao, Siddharth Vats, Sachidanand Singh
Summary: This study aims to identify key proteins that regulate both resistance and virulence in Porphyromonas gingivalis, which could be used as drug targets against antibiotic-resistant infections. By constructing a protein interaction network and using various analysis methods, several important genes were identified that have significance in treating periodontal diseases and studying antibiotic resistance and pathogenicity in bacteria other than P. gingivalis.
Article
Immunology
Si Chen, ChenGuang Niu, WanQi Lv
Summary: This study demonstrated for the first time the alteration of fungal composition and function with P. gingivalis administration, and investigated the interactions between fungi and bacteria, as well as between fungi and metabolites. The findings provide a comprehensive insight into the remodeling of the gut mycobiome by oral pathogens through multi-omics analyses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Remberto Marcelo Argandona Valdez, Laurie Ann Ximenez-Fyvie, Karina Sampaio Caiaffa, Vanessa Rodrigues dos Santos, Rina Maria Gonzales Cervantes, Argelia Almaguer-Flores, Cristiane Duque
Summary: The study found that Bifidobacterium infantis and Bifidobacterium lactis alone or in double combinations have antagonist effects on periodontopathogens biofilms at different time points, with minimal influence on S. oralis growth.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
Anastasios Karydis, Indra Sandal, Jiwen Luo, Amanda Prislovsky, Amanda Gamboa, Edward F. Rosloniec, David D. Brand
Summary: The study found that carrying the shared epitope and inoculation with Porphyromonas gingivalis increased the percentage of Th17 cells, significantly increased bone loss, and allowed for the generation of CCP2 ACPAs not found in C57BL/6 or DBA/1 arthritic mouse serum.
Article
Polymer Science
Wei-Fang Lee, Lu-Ying Wang, Ting-Yi Renn, Jen-Chang Yang, Lih-Sheng Fang, Yi-Huan Lee, Pei-Wen Peng
Summary: In this study, a polymeric substrate PEKK coated with 0.5% AgNPs was successfully synthesized and demonstrated to have antibacterial properties, making it suitable for implant applications.
Article
Multidisciplinary Sciences
Maribasappa Karched, Radhika G. Bhardwaj, Muawia Qudeimat, Areej Al-Khabbaz, Arjuna Ellepola
Summary: This study aimed to characterize the secretome of Prevotella intermedia, an important species associated with periodontitis, in biofilm and planktonic life mode. The results showed significant differences between the two modes, with the biofilm secretome having higher protein content and cytokine-inducing ability.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Shuhui Zhou, Yaoting Ji, Hantao Yao, Haiying Guo, Zichen Zhang, Zijun Wang, Minquan Du
Summary: This study found that ginsenoside Rd has inhibitory effects on periodontitis-related bacteria and inflammatory response, as well as the ability to inhibit bone resorption induced by periodontitis. The results provide potential evidence for the development of Rd as a medication for the control and prevention of periodontitis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Mohamed Omar, Maria Alexiou, Umar R. Rekhi, Konrad Lehmann, Aneesh Bhardwaj, Cole Delyea, Shokrollah Elahi, Maria Febbraio
Summary: Periodontitis, the leading cause of adult tooth loss, has been identified as an independent risk factor for cardiovascular disease (CVD). The study used a BM transplant approach to simulate the clinical elimination of periodontitis and found that recipients of BM from Pg-inoculated donors developed significantly more atherosclerosis. The research suggests that periodontitis induces epigenetic changes in hematopoietic stem cells, leading to long-term association between periodontitis and atherosclerotic CVD.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Engineering, Chemical
Deepavalli Arumuganainar, Gopinath Subramanian, Santhosh Basavarajappa, Mohamed Ibrahim Hashem, Kurumathur Vasudevan Arun, Subbusamy Kanakasabapathy Balaji, Pradeep Kumar Yadalam, Baskar Venkidasamy, Ramachandran Vinayagam
Summary: Periodontal diseases are common oral conditions with severe complications. They are caused by the oral microbiome and influenced by factors such as aging, tobacco usage, systemic illness, and inadequate oral hygiene. Plant-derived phytochemicals, such as those found in Solanum xanthocarpum, have potential in preventing and managing periodontal diseases due to their antioxidant, anti-inflammatory, and antibacterial activities.
Article
Multidisciplinary Sciences
Gina R. Lewin, Kendall S. Stocke, Richard J. Lamont, Marvin Whiteley
Summary: This study characterizes the behavior and virulence of the periodontal pathogen Porphyromonas gingivalis during human infection. It reveals that P. gingivalis is highly expressed in diseased samples, with genes related to virulence factors and growth/matabolism being actively expressed. The study also shows that the gene expression of P. gingivalis during periodontitis is highly conserved with logarithmic laboratory growth, suggesting that simple test tube growth can be used as a model for studying P. gingivalis biology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Seonhwa Park, Kiryeon Park, Hee Sam Na, Jin Chung, Haesik Yang
Summary: An electrochemical biosensor for sensitive detection of Porphyromonas gingivalis in saliva was developed without the need for washing and separation steps. This biosensor can be used as an initial diagnosis method to distinguish between healthy people and patients with periodontal diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Joseph Aduse-Opoku, Susan Joseph, Deirdre A. Devine, Philip D. Marsh, Michael A. Curtis
Summary: The periodontal pathogen Porphyromonas gingivalis is genetically heterogeneous and can spontaneously generate phenotypically different sub-strains. These variants exhibit altered phenotypic properties and attenuated virulence in a mouse model, possibly due to genetic changes at specific loci and regions in the genome.
MOLECULAR ORAL MICROBIOLOGY
(2022)
Article
Microbiology
Katarzyna Szczesniak, Florian Veillard, Carsten Scavenius, Kamila Chudzik, Kinga Ferenc, Matthias Bochtler, Jan Potempa, Danuta Mizgalska
Summary: In certain bacteria, the glutaminyl cyclase (QC) is crucial for protein stability and bacterial growth. QC converts glutamine residues to pyroglutamate, protecting proteins from degradation. However, the loss of QC results in the loss of Q-rule substrates, leading to the inability of bacteria to survive.
Article
Biochemistry & Molecular Biology
Kanika Arora, Bhishem Thakur, Archit Gupta, Purnananda Guptasarma
Summary: In enteric bacteria like Escherichia coli, there are two homologs of the DNA-binding nucleoid associated protein (NAP) known as HU, which exist in different forms and dominate different stages of bacterial growth. Due to similarities in their properties and the difficulty in purifying them separately, these dimeric forms can interfere with each other. The creation of a functional analog of HU-AB, termed HU-B-A, solves this problem by combining HU-B and HU-A chains into a single polypeptide with a higher stability and dominance in mixtures.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Microbiology
Mariusz Madej, Zuzanna Nowakowska, Miroslaw Ksiazek, Anna M. Lasica, Danuta Mizgalska, Magdalena Nowak, Anna Jacula, Monika Bzowska, Carsten Scavenius, Jan J. Enghild, Joseph Aduse-Opoku, Michael A. Curtis, F. Xavier Gomis-Ruth, Jan Potempa
Summary: The T9SS component PorZ interacts with sortase and specifically binds A-LPS, mediated by a phosphorylated branched mannan repeat in A-LPS polysaccharide. ALPS-bound PorZ interacts with sortase with significantly higher affinity, facilitating modification of cargo proteins by the cell surface attachment complex of the T9SS.
Article
Dentistry, Oral Surgery & Medicine
C. J. Rocco, M. E. Davey, L. O. Bakaletz, S. D. Goodman
MOLECULAR ORAL MICROBIOLOGY
(2017)
Article
Multidisciplinary Sciences
Daniela Boernigen, Boyu Ren, Robert Pickard, Jingfeng Li, Enver Ozer, Erica M. Hartmann, Weihong Xiao, Timothy Tickle, Jennifer Rider, Dirk Gevers, Eric A. Franzosa, Mary Ellen Davey, Maura L. Gillison, Curtis Huttenhower
SCIENTIFIC REPORTS
(2017)
Article
Biotechnology & Applied Microbiology
Zachary D. Moye, Courtney M. Gormley, Mary E. Davey
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Ecology
M. Fata Moradali, Shirin Ghods, Thomas E. Angelini, Mary Ellen Davey
Article
Biotechnology & Applied Microbiology
Danielle M. Vermilyea, Gregory K. Ottenberg, Mary E. Davey
NPJ BIOFILMS AND MICROBIOMES
(2019)
Article
Dentistry, Oral Surgery & Medicine
F. G. Rocha, Z. D. Moye, G. Ottenberg, P. Tang, D. J. Campopiano, F. C. Gibson, M. E. Davey
JOURNAL OF DENTAL RESEARCH
(2020)
Article
Biotechnology & Applied Microbiology
Hey-Min Kim, Mary E. Davey
NPJ BIOFILMS AND MICROBIOMES
(2020)
Article
Immunology
Fernanda G. Rocha, Gregory Ottenberg, Zavier G. Eure, Mary E. Davey, Frank C. Gibson
Summary: The study reveals that P. gingivalis delivers sphingolipids to host cells via OMVs, which can limit host inflammatory response. A mutant lacking sphingolipids induces a stronger inflammatory immune response compared to the wild-type strain.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Danielle M. Vermilyea, M. Fata Moradali, Hey-Min Kim, Mary E. Davey
Summary: Many bacteria can switch between sessile and motile modes in response to signals, and recent studies have shown Porphyromonas gingivalis can surface translocate. PPAD secreted by P. gingivalis promotes this process by impacting OMV biogenesis. OMVs containing enzymes like gingipains indicate PPAD's role in periodontitis and systemic diseases.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
M. Fata Moradali, Mary E. Davey
Summary: The study shows that Porphyromonas gingivalis has metabolic plasticity and can utilize non-proteinaceous substrates for colonization and biofilm formation, promoting biofilm development and increasing invasion of gingival epithelial cells. The experiments reveal the mechanisms by which P. gingivalis uses different substrates for anabolism and upregulates fimbrial adhesion.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Microbiology
Dev K. Ranjit, Zachary D. Moye, Fernanda G. Rocha, Gregory Ottenberg, Frank C. Nichols, Hey-Min Kim, Alejandro R. Walker, Frank C. Gibson, Mary E. Davey
Summary: This study reports a novel kinase in Porphyromonas gingivalis that phosphorylates dihydrosphingosine to form dhS1P, and regulates the lipid profile of P. gingivalis. This finding is of great importance for studying immune modulation mechanisms.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Hey-Min Kim, Christina Magda Rothenberger, Mary Ellen Davey
Summary: Studies have shown that the stress hormone cortisol can affect gene expression and surface translocation of the oral microbiome, specifically in the presence of certain nutrients. The results indicate that cortisol promotes migration of Porphyromonas gingivalis and up-regulates the expression of T9SS-associated genes. These findings suggest a potential link between the stress hormone cortisol, surface translocation, and virulence of P. gingivalis.
Article
Dentistry, Oral Surgery & Medicine
Frank C. C. Nichols, Kruttika Bhuse, Robert B. B. Clark, Anthony A. A. Provatas, Elena Carrington, Yu-Hsiung Wang, Qiang Zhu, Mary E. E. Davey, Floyd E. E. Dewhirst
Summary: The study found that serine/glycine lipids from oral pathogens are more abundant in subgingival calculus, diseased teeth, and diseased gingival tissues, compared to healthy gingival tissues.
FRONTIERS IN ORAL HEALTH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Fernanda G. Rocha, Aym Berges, Angie Sedra, Shirin Ghods, Neeraj Kapoor, Lucy Pill, Mary Ellen Davey, Jeff Fairman, Frank C. Gibson
Summary: Periodontal diseases are a common cause of tooth loss among adults, with Porphyromonas gingivalis being a key etiological organism. Research shows that a conjugate vaccine incorporating capsular polysaccharide antigen is effective in preventing oral bone destruction caused by periodontal diseases.
FRONTIERS IN ORAL HEALTH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hey-Min Kim, Dev K. Ranjit, Alejandro R. Walker, Heran Getachew, Ann Progulske-Fox, Mary E. Davey
Summary: The response regulator PG0720 indirectly influences the synthesis of Porphyromonas gingivalis surface glycans by activating the expression of asSuGR, a trans-acting antisense RNA molecule. Deletion of the PG0720 gene results in down-regulation of genes involved in K-antigen capsule synthesis and reduced virulence in the mutant strain. Complementation of PG0720 deletion mutant restores the defects in surface polysaccharide presentation, indicating the important regulatory role of PG0720 in modulating the production of surface polysaccharides in P. gingivalis strain W83.
FRONTIERS IN ORAL HEALTH
(2021)