Soluble Prokaryotic Overexpression and Purification of Bioactive Human Granulocyte Colony-Stimulating Factor by Maltose Binding Protein and Protein Disulfide Isomerase

Title
Soluble Prokaryotic Overexpression and Purification of Bioactive Human Granulocyte Colony-Stimulating Factor by Maltose Binding Protein and Protein Disulfide Isomerase
Authors
Keywords
Solubility, Cell fusion, Proteases, Silver staining, Protein expression, Endotoxins, Cytoplasm, Sonication
Journal
PLoS One
Volume 9, Issue 3, Pages e89906
Publisher
Public Library of Science (PLoS)
Online
2014-03-04
DOI
10.1371/journal.pone.0089906

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