Article
Biochemistry & Molecular Biology
Ljubava D. Zorova, Sergei I. Kovalchuk, Vasily A. Popkov, Valery P. Chernikov, Anastasia A. Zharikova, Anastasia A. Khutornenko, Savva D. Zorov, Konstantin S. Plokhikh, Roman A. Zinovkin, Ekaterina A. Evtushenko, Valentina A. Babenko, Irina B. Pevzner, Yulia A. Shevtsova, Kirill V. Goryunov, Egor Y. Plotnikov, Denis N. Silachev, Gennady T. Sukhikh, Dmitry B. Zorov
Summary: Extracellular vesicles derived from stem cells have been widely studied for their potential use in cell therapy. This study examined two fractions of vesicles derived from mesenchymal stromal stem cells and found the presence of mitochondrial components in these vesicles. However, it is still unclear whether these mitochondria are fully functional inside the vesicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Hassan Dianat-Moghadam, Mostafa Khalili, Mohsen Keshavarz, Mehdi Azizi, Hamed Hamishehkar, Reza Rahbarghazi, Mohammad Nouri
Summary: The study investigated the impact of LXR on the tumorgenicity of human colorectal CD133 cells by incubating them with LXR agonist and antagonist. Activation of LXR up-regulated ABC transporters expression, but suppressed proliferation, clonogenicity, and migration of CD133 cells. Inhibition of LXR decreased metabolic enzyme levels and increased intracellular ROS, inducing apoptotic changes in cancer stem cells. The regulation of LXR activity may potentially target survival, metabolism, and migration in CSCs to control tumorigenesis and metastasis in advanced colorectal cancers.
CANCER CELL INTERNATIONAL
(2021)
Article
Physics, Multidisciplinary
Thomas Fuhs, Franziska Wetzel, Anatol W. Fritsch, Xinzhi Li, Roland Stange, Steve Pawlizak, Tobias R. Kiessling, Erik Morawetz, Steffen Grosser, Frank Sauer, Juergen Lippoldt, Frederic Renner, Sabrina Friebe, Mareike Zink, Klaus Bendrat, Juergen Braun, Maja H. Oktay, John Condeelis, Susanne Briest, Benjamin Wolf, Lars-Christian Horn, Michael Hoeckel, Bahriye Aktas, M. Cristina Marchetti, M. Lisa Manning, Axel Niendorf, Dapeng Bi, Josef A. Kaes
Summary: Cancer cells within tumors tend to become softer, and primary tumors exhibit high mechanical heterogeneity across scales, providing stability and room for cell motility.
Review
Biochemistry & Molecular Biology
Qiang Zong, Katrin Bundkirchen, Claudia Neunaber, Sandra Noack
Summary: Bone marrow-derived mesenchymal stem cells (BMSCs) have potential for cell-based therapies, but their properties can be affected by the overweight/obesity microenvironment. This review summarizes evidence on the effects of overweight/obesity on BMSC characteristics. Existing studies show inconsistent results and the mechanisms involved are still unclear. Further research is needed to improve the functions of overweight/obesity-derived BMSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Hassan Dianat-Moghadam, Soheil Abbasspour-Ravasjani, Hamed Hamishehkar, Reza Rahbarghazi, Mahammad Nouri
Summary: Reprogrammed metabolism and active stemness are important for the survival and tumor formation of cancer stem cells (CSCs). The LXR signaling pathway regulates metabolism in different types of cancers. An inhibitor called SR9243 (SR) can specifically target and eliminate non-CSC tumor cells. CD133 is a marker for CSCs in solid tumors and is associated with active lipogenesis. The use of anti-CD133 monoclonal antibodies can enhance drug delivery systems and improve the effectiveness of drugs with poor water solubility, such as SR. In this study, targeted immunoliposomes (ILipo) were developed to deliver SR specifically to CD133 + CSCs and induce their functional effects. The results showed that ILipo, with an average size of 64.79 nm, can encapsulate SR effectively and bind to CSCs. Flow cytometry analysis demonstrated that ILipo was taken up more efficiently by CD133 + CSCs compared to non-targeted liposomes. ILipo-SR was significantly more toxic to CD133 + CSCs compared to free SR and non-targeted liposomes. ILipo-SR also reduced clonogenicity, stemness, and lipogenesis in CD133 + CSCs, increased ROS generation, and induced apoptosis more effectively than Lipo-SR. This study suggests that targeting CD133 and LXR simultaneously could be a promising strategy for targeting CD133 + CSCs with dynamic metabolism and self-renewal potentials.
Article
Cell Biology
Tomohiro Iriki, Hiroaki Iio, Shu Yasuda, Shun Masuta, Masakazu Kato, Hidetaka Kosako, Shoshiro Hirayama, Akinori Endo, Fumiaki Ohtake, Mako Kamiya, Yasuteru Urano, Yasushi Saeki, Jun Hamazaki, Shigeo Murata
Summary: Proteasome plays a role in senescent cells by forming nuclear foci with liquid-like properties. These foci, known as senescence-associated nuclear proteasome foci (SANPs), require ubiquitination, RAD23B, and proteasome activity for their formation. Knock-down of RAD23B suppresses SANP formation and alters mitochondrial activity, resulting in increased reactive oxygen species production.
Article
Oncology
Hugo Passos Vicari, Juan Luiz Coelho-Silva, Diego A. Pereira-Martins, Antonio Roberto Lucena-Araujo, Keli Lima, Jean Carlos Lipreri da Silva, Priscila Santos Scheucher, Luisa C. Koury, Raul A. de Melo, Rosane Bittencourt, Katia Pagnano, Elenaide Nunes, Evandro M. Fagundes, Fabio Kerbauy, Lorena Lobo De Figueiredo-Pontes, Leticia Veras Costa-Lotufo, Eduardo Magalhaes Rego, Fabiola Traina, Joao Agostinho Machado-Neto
Summary: This study found that the expression levels of STMN1 were significantly increased in APL patients, associated with proliferation- and metabolism-related gene signatures. STMN1 was highly expressed in early hematopoietic progenitors and reduced during cell differentiation. Silencing STMN1 could decrease autonomous cell growth, while it did not impact ATRA-induced apoptosis and differentiation.
INVESTIGATIONAL NEW DRUGS
(2022)
Article
Multidisciplinary Sciences
Maria C. Basil, Fabian L. Cardenas-Diaz, Jaymin J. Kathiriya, Michael P. Morley, Justine Carl, Alexis N. Brumwell, Jeremy Katzen, Katherine J. Slovik, Apoorva Babu, Su Zhou, Madison M. Kremp, Katherine B. McCauley, Shanru Li, Joseph D. Planer, Shah S. Hussain, Xiaoming Liu, Rebecca Windmueller, Yun Ying, Kathleen M. Stewart, Michelle Oyster, Jason D. Christie, Joshua M. Diamond, John F. Engelhardt, Edward Cantu, Steven M. Rowe, Darrell N. Kotton, Harold A. Chapman, Edward E. Morrisey
Summary: This study reveals the existence of a unique population of secretory cells in the respiratory bronchioles of the human lung, which act as unidirectional progenitors for alveolar type 2 cells. The differentiation of these respiratory airway secretory cells into alveolar type 2 cells is regulated by Notch and Wnt signaling. These findings highlight the importance of this distinct progenitor cell population in maintaining the gas-exchange compartment and its potential role in chronic lung diseases.
Article
Gastroenterology & Hepatology
Vincenzo Cardinale, Guido Carpino, Diletta Overi, Samira Safarikia, Wencheng Zhang, Matt Kanke, Antonio Franchitto, Daniele Costantini, Olga Riccioni, Lorenzo Nevi, Michele Chiappetta, Paolo Onori, Matteo Franchitto, Simone Bini, Yu-Han Hung, Quirino Lai, Ilaria Zizzari, Marianna Nuti, Carmine Nicoletti, Saula Checquolo, Laura Di Magno, Maria Valeria Giuli, Massimo Rossi, Praveen Sethupathy, Lola M. Reid, Domenico Alvaro, Eugenio Gaudio
Summary: Stem/progenitor cells with regenerative potential have been identified in the adult human duodenum. These cells can form organoids in vitro and differentiate into mature liver cells in vivo. The duodenal submucosal gland cells could potentially serve as a novel cell source for regenerative medicine of the liver.
JOURNAL OF HEPATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
X. Lin, H. Zhang, J. Liu, C. L. Wu, A. McDavid, B. F. Boyce, L. Xing
Summary: This study identified three major clusters in callus SSPCs, confirming their heterogeneity, and importantly, increased IRF/NF-kappa B-mediated inflammatory osteogenic population with decreased osteogenic potential in aged cells.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Oncology
Tania Capeloa, Joanna Krzystyniak, Donatienne D'Hose, Amanda Canas Rodriguez, Valery L. Payen, Luca X. Zampieri, Justine A. Van de Velde, Zohra Benyahia, Erica Pranzini, Thibaut Vazeille, Maude Fransolet, Caroline Bouzin, Davide Brusa, Carine Michiels, Bernard Gallez, Michael P. Murphy, Paolo E. Porporato, Pierre Sonveaux
Summary: This study investigates the potential of the mitochondria-targeted antioxidant MitoQ in inhibiting breast cancer metastasis. Using human breast cancer cells as models, the researchers demonstrate that MitoQ can inhibit cancer cell migration, invasion, clonogenicity, sphere formation, and spheroid stability. The findings of this study are important for future clinical research and evaluation of MitoQ for the prevention of breast cancer metastasis.
Review
Biology
Keshia Pitt, Yoshiyuki Mochida, Makoto Senoo
Summary: The work of American scientist Howard Green has had a profound impact on the field of epithelial stem cell development, as his breakthroughs in stem cell culture methods have been translated into therapeutic practice. This review chronicles the milestones that have propelled the field of regenerative medicine for the skin forward over the past fifty years. It details Green's early discoveries, highlights clinical cases that have made life-saving use of his findings, and discusses the achievements of other scientists who built upon his discoveries.
Article
Multidisciplinary Sciences
Simone Isling Paerregaard, Line Wulff, Sophie Schussek, Kristoffer Niss, Urs Morbe, Johan Jendholm, Kerstin Wendland, Anna T. Andrusaite, Kevin F. Brulois, Robert J. B. Nibbs, Katarzyna Sitnik, Allan McI Mowat, Eugene C. Butcher, Sren Brunak, William W. Agace
Summary: The intestinal lamina propria contains diverse fibroblast subsets that are located in specific anatomical niches and exhibit region-specific functions. By analyzing transcriptional profiles and conducting transplantation and lineage-tracing experiments, this study reveals the ontogeny and heterogeneity of intestinal fibroblasts, as well as the functional diversity of colonic subepithelial fibroblasts.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Clemens Zwergel, Michele Aventaggiato, Sabrina Garbo, Elisabetta Di Bello, Bruno Fassari, Beatrice Noce, Carola Castiello, Chiara Lambona, Federica Barreca, Dante Rotili, Rossella Fioravanti, Thomas Schmalz, Michael Weyand, Amelie Niedermeier, Marco Tripodi, Gianni Colotti, Clemens Steegborn, Cecilia Battistelli, Marco Tafani, Sergio Valente, Antonello Mai
Summary: The mitochondrial protein SIRT3 plays a role in cancer, metabolism, and hypoxia-related diseases. New 1,4-dihydropyridine compounds, namely 2 and 3, have been discovered and compound 3 is a specific activator of SIRT3. Among a series of related compounds, compound 3c binds and activates SIRT3 the strongest, while compound 3d shows the best effects on enhancing glutamate dehydrogenase activity and deacetylating proteins in breast cancer cells. Compound 3d also exhibits significant anti-cancer effects in both normoxia and hypoxia conditions, reducing cell viability, clonogenicity, and migration ability.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Aleksandrs Nasonovs, Miguel Garcia-Diaz, Daniel F. Bogenhagen
Summary: The research discovered that A549 human lung carcinoma cells often have swollen mitochondria containing multiple discrete mtDNA nucleoids and RNA processing granules. These abnormal mitochondrial structures, known as mito-bulbs, are similar to those described in cells exposed to various genomic stressors, but are also observed under typical culture conditions and tend to have elevated oxidative environments.
Article
Pharmacology & Pharmacy
Terry Petrou, Hervor L. Olsen, Christopher Thrasivoulou, John R. Masters, Jonathan F. Ashmore, Aamir Ahmed
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2017)
Article
Multidisciplinary Sciences
Antonio Giuliano, Rebecca Swift, Callum Arthurs, Georgina Marote, Francesca Abramo, Jenny McKay, Calum Thomson, Mariana Beltran, Michael Millar, Simon Priestnall, Jane Dobson, Fernando Costantino-Casas, Terry Petrou, Imelda M. McGonnell, Anthony J. Davies, Malcolm Weetman, Oliver A. Garden, John R. Masters, Christopher Thrasivoulou, Aamir Ahmed
Review
Cell Biology
Maria Notara, Aamir Ahmed
CELL BIOLOGY AND TOXICOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Christopher Thrasivoulou, Michael Millar, Aamir Ahmed
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Multidisciplinary Sciences
Qin Wang, Andrew J. Symes, Corrina A. Kane, Alex Freeman, Joseph Nariculam, Philippa Munson, Christopher Thrasivoulou, John R. W. Masters, Aamir Ahmed
Article
Multidisciplinary Sciences
Hidekazu Yamamoto, John R. Masters, Prokar Dasgupta, Ashish Chandra, Rick Popert, Alex Freeman, Aamir Ahmed
Article
Multidisciplinary Sciences
Manit Arya, Christopher Thrasivoulou, Rui Henrique, Michael Millar, Ruth Hamblin, Reena Davda, Kristina Aare, John R. Masters, Calum Thomson, Asif Muneer, Hitendra R. H. Patel, Aamir Ahmed
Article
Multidisciplinary Sciences
Callum Arthurs, Bibi Nazia Murtaza, Calum Thomson, Kerry Dickens, Rui Henrique, Hitendra R. H. Patel, Mariana Beltran, Michael Millar, Christopher Thrasivoulou, Aamir Ahmed
Review
Oncology
J. R. Masters, C. Kane, H. Yamamoto, A. Ahmed
PROSTATE CANCER AND PROSTATIC DISEASES
(2008)
Article
Pharmacology & Pharmacy
Marta Reyes-Corral, Naja M. Sorensen, Christopher Thrasivoulou, Prokar Dasgupta, Jonathan F. Ashmore, Aamir Ahmed
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2019)
Article
Neurosciences
Jonathan Ashmore, Hervor Olsen, Naja Sorensen, Christopher Thrasivoulou, Aamir Ahmed
JOURNAL OF PHYSIOLOGY-LONDON
(2019)
Review
Urology & Nephrology
Alexander Light, Aamir Ahmed, Prokar Dasgupta, Oussama Elhage
Article
Multidisciplinary Sciences
Callum Arthurs, Alejandro Suarez-Bonnet, Claire Willis, Boyu Xie, Natalie Machulla, Tim S. Mair, Kevin Cao, Michael Millar, Christopher Thrasivoulou, Simon L. Priestnall, Aamir Ahmed
SCIENTIFIC REPORTS
(2020)
Article
Medicine, General & Internal
Kevin Cao, Callum Arthurs, Ali Atta-ul, Michael Millar, Mariana Beltran, Jochen Neuhaus, Lars-Christian Horn, Rui Henrique, Aamir Ahmed, Christopher Thrasivoulou