Article
Biochemistry & Molecular Biology
Feijie Liu, Yueqiao Wang, Guhua Zhang, Longna Li, Wenbiao Shen
Summary: This study found that endogenous hydrogen gas (H-2) can promote lateral root (LR) formation, and this process is likely regulated through cell cycle regulation and auxin-dependent pathway.
Article
Agronomy
Dandan Huang, Yuanyi Wang, Dingchuan Zhang, Yingfei Dong, Qingxi Meng, Shuhua Zhu, Lili Zhang
Summary: The study found that exogenous strigolactone can alleviate oxidative damage of strawberries by improving the defense capability of the antioxidant system and promoting related metabolic activities, maintaining fruit quality.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Engineering, Environmental
Yueqiao Wang, Peixun Lv, Lingshuai Kong, Wenbiao Shen, Qianjun He
Summary: The hydrogen-releasing nanomaterial AB@hMSN shows high hydrogen loading capacity and sustained hydrogen releasing behavior in slightly acidic soil environments, providing plentiful amounts of hydrogen gas to sustainably induce tomato lateral root formation, indicating its potential application in agriculture.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Spectroscopy
Yue Xin, Mei Wang, Mengfei Liu, Yinuo Chen, Han Zhao, Pingzhu Zhang, Xiaoliu Li, Chao Wei
Summary: In this study, three BODIPY-NBD dyads were designed and synthesized as probes for highly selective detection of H2S. Among them, the o-BNP probe showed the highest fluorescence enhancement and the lowest detection limit. The o-BNP probe can visualize the production of endogenous H2S in HeLa cells and zebrafish.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Spectroscopy
Qi Sun, Heng Liu, Yuan Qiu, Jun Chen, Feng-Shou Wu, Xiao-Gang Luo, Da-Wei Wang
Summary: NT-SH is a fluorescence turn-on probe for sensing hydrogen sulfide, showing excellent detection performance with good water solubility and low cytotoxicity. The response mechanism of NT-SH for H2S was confirmed, and it was demonstrated to have low toxicity and good biocompatibility in vitro and in vivo.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Applied
Zhuye Shang, Qingtao Meng, Dihua Tian, Yue Wang, Zexi Zhang, Zhiqiang Zhang, Run Zhang
Summary: We have developed a red-emitting fluorescence probe (XDS) for detection of hydrogen sulfide (H2S) in biosystems and real-world food samples. The probe exhibits remarkable fluorescence quenching in response to H2S and can be used for semi-quantitative detection of H2S in water and beer samples, as well as real-time monitoring of H2S production during food spoilage. In addition, XDS is low toxicity and can be used for visualizing endogenous and exogenous H2S in vivo in a mouse model. This development provides an effective tool for studying the roles of H2S in biomedical systems and for food safety evaluation in the future.
Review
Biochemistry & Molecular Biology
Hua Li, Hongyu Chen, Lulu Chen, Chenyang Wang
Summary: This review summarizes the regulating effects of hydrogen sulfide (H2S) on root system architecture, including primary root growth, lateral and adventitious root formation, root hair development, and nodule formation. The review also discusses the genes involved in the regulation of root system architecture by H2S and their relationships with other signal pathways, as well as how H2S regulates root growth in response to abiotic stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Pei Chen, Wanxia Yang, Minxue Wen, Songheng Jin, Yang Liu
Summary: The study demonstrated that H2S pretreatment maintained chlorophyll fluorescence and attenuated plant biomass loss under salt stress. Furthermore, H2S pretreatment increased endogenous NO content and nitrate reductase activity, alleviating salt-induced oxidative damage. The involvement of H2S-induced NO in Cyclocarya paliurus salt tolerance was confirmed through the study.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Engineering, Environmental
Yan Feng, Shanshan Hu, Yingzhe Wang, Xuerui Song, Chen Cao, Kun Wang, Chunling Jing, Guolin Zhang, Weisheng Liu
Summary: A bifunctional tri-site fluorescent probe was designed for visualization and quantitative analysis of H2S in wastewater and sensitive monitoring of GSH in living cells. The recognition properties of GSH towards H2S/GSH were demonstrated through various analyses. Integrated with a paper strip, a portable smartphone-sensing platform showed potential for cost-effective and rapid colorimetric water quality testing in environmental monitoring.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Ben Liu, Jing Long, Meng Zhang, Kaiming Cheng, Xin Gao, Yibo Zhou, Yi Li, Zilong Tang, Wei Zhang
Summary: This study successfully achieved the goal of detecting H2S in mitochondria through exploring an iridium complex, which displays high selectivity and sensitivity, low cell toxicity, and has been successfully used for in vitro and in vivo H2S imaging.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Yang Xu, Hongmei Yu, Lalit Chudal, Nil Kanatha Pandey, Eric Horacio Amador, Brian Bui, Lingyun Wang, Xuedan Ma, Shuping Deng, Xiuhui Zhu, Shaoyan Wang, Wei Chen
Summary: In this study, carbon dots passivated with polyethylene glycol were explored as a highly sensitive H2S detection probe, exhibiting different luminescent properties when excited at different wavelengths. The interaction of Na2S with CDs led to a linear increase in blue emission at 455 nm. The method showed excellent linear relationships and low detection limits, making it a promising approach for sensitive H2S detection and bioimaging applications.
MATERIALS TODAY PHYSICS
(2021)
Article
Chemistry, Analytical
Xue Yu, Yunong Huang, Ning Zhang, Qi Zan, Xiaodong Wang, Zhuping Jin, Li Fan, Chuan Dong, Yuewei Zhang
Summary: A LD-targeting and H2S-activated probe with excellent specificity and low toxicity was designed and developed. The probe showed excellent imaging performance in living cells, cancer tissues, and Arabidopsis seedlings, demonstrating potential for cancer diagnosis. Additionally, a smartphone-integrated platform for H2S analysis was successfully established.
ANALYTICA CHIMICA ACTA
(2023)
Review
Engineering, Biomedical
He Ding, Jinhu Chang, Fei He, Shili Gai, Piaoping Yang
Summary: Advances in nanotechnology have led to the rapid development of therapeutic nanomaterials for gas therapy, particularly with H2S-responsive properties. The interdisciplinary study of H2S is a scientific hotspot exploring its chemical properties, biological mechanisms, and therapeutic effects, showcasing potential for treating diseases in biological systems.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Analytical
Zimeng Li, Jingshuo Gao, Zhenbo Guo, Han Zhao, Liyan Liu, Mei Wang, Pingzhu Zhang, Guofeng Chen, Xiaoliu Li, Chao Wei
Summary: In this study, a dual-locked near-infrared fluorescent probe was developed for monitoring the fluctuation of H2S in vitro and in vivo. The probe showed high selectivity and sensitivity to H2S compared to single-locked probes. Using this probe, visualization of endogenous H2S production in insulin-resistant cells and the fluctuation of H2S in diabetic mice was achieved for the first time.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Jinlong Zhang, Shuai Mu, Weilin Wang, Huipeng Sun, Shuangqin Li, Xuezhao Shi, Yunbo Liu, Xiaoyan Liu, Haixia Zhang
Summary: In this study, a novel fluorescent probe was developed for the detection of H2S. The probe showed comparable imaging performance to the traditional probe but exhibited lower toxicity to cells and animals.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Applied
Ali Wang, Peng Li, Peipei Han, Gan Gu, Tijiang Shan, Daowan Lai, Ligang Zhou
Summary: New nitrogen-containing metabolites and natural products were isolated from the rice false smut pathogen, Villosiclava virens UV-8b, and evaluated for antibacterial and phytotoxic activities. Some compounds showed weak inhibition against bacteria, while others displayed inhibitory activity against the radicle elongation of rice seeds.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Applied
Jiajia Meng, Siji Zhao, Pengqin Dang, Zhiyao Zhou, Daowan Lai, Ligang Zhou
Summary: Ustilaginoidin M-1, a novel bis-naphtho-gamma-pyrone isolated from the pathogenic fungus Villosiclava virens, was tested for cytotoxicity against five human cancer cell lines after its structure was elucidated through spectroscopic analysis and comparison with literature data.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Applied
Daowan Lai, Jing Li, Siji Zhao, Gan Gu, Xiao Gong, Peter Proksch, Ligang Zhou
Summary: Several compounds isolated from the endophytic fungus Xylomelasma sp. Samif07 showed significant antibacterial, antitubercular, and antioxidant activities, indicating their potential as natural products for drug development.
NATURAL PRODUCT RESEARCH
(2021)
Article
Multidisciplinary Sciences
Daowan Lai, Ziling Mao, Zhiyao Zhou, Siji Zhao, Mengyao Xue, Jungui Dai, Ligang Zhou, Dianpeng Li
SCIENTIFIC REPORTS
(2020)
Article
Microbiology
Zheng Hu, Lu Zheng, Junbin Huang, Ligang Zhou, Chunsheng Liu, Hao Liu
CURRENT MICROBIOLOGY
(2020)
Review
Food Science & Technology
Dan Xu, Mengyao Xue, Zhen Shen, Xiaowei Jia, Xuwen Hou, Daowan Lai, Ligang Zhou
Summary: Fungal phytotoxic secondary metabolites are toxic substances produced by fungi through biochemical reactions, with a high level of diversity in their properties. These metabolites are mainly isolated from phytopathogenic fungal species, with aromatic polyketides and sesquiterpenoids being the main phytotoxic compounds.
Article
Microbiology
Xuwen Hou, Xuping Zhang, Mengyao Xue, Zhitong Zhao, Huizhen Zhang, Dan Xu, Daowan Lai, Ligang Zhou
Summary: This review summarizes the structures and bioactivities of 69 sorbicillinoids newly identified from 2016 to 2021. These compounds are classified into different categories and exhibit various biological activities, including cytotoxic, antimicrobial, anti-inflammatory, and phytotoxic effects.
Article
Biochemistry & Molecular Biology
Xuping Zhang, Dan Xu, Xuwen Hou, Penglin Wei, Jiajin Fu, Zhitong Zhao, Mingpeng Jing, Daowan Lai, Wenbing Yin, Ligang Zhou
Summary: This study identified a sorbicillinoid biosynthetic gene cluster in U. virens and demonstrated that UvSorA and UvSorB are responsible for sorbicillinoid biosynthesis and play important roles in the fungal response to external stresses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Weixuan Wang, Gan Gu, Ruya Yin, Jiajin Fu, Mingpeng Jing, Zhen Shen, Daowan Lai, Baomin Wang, Ligang Zhou
Summary: In this study, a nanobody phage display library was established and two nanobodies were selected for the development of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to detect ustilaginoidins in rice. Nb-B15 showed superior characteristics compared to Nb-C21 and was chosen for the detection of actual contaminated rice samples. The developed ic-ELISA based on Nb-B15 showed consistent results with HPLC-DAD analysis, suggesting it can be a rapid and effective method for detecting total ustilaginoidins in contaminated rice samples.
Review
Microbiology
Mengyao Xue, Xuwen Hou, Jiajin Fu, Jiayin Zhang, Jiacheng Wang, Zhitong Zhao, Dan Xu, Daowan Lai, Ligang Zhou
Summary: Genomic analysis has identified cryptic biosynthetic gene clusters in fungi that are normally silenced but can be induced using chemical-epigenetic modifiers. These modifiers, such as 5-azacytidine and suberoylanilide hydroxamic acid, alter the structure of DNA and histones to activate the production of bioactive secondary metabolites. This review summarizes the research progress from 2007 to 2022 on using chemical epigenetic modifiers to trigger silent or low-expressed biosynthetic pathways in fungi, resulting in the production of over 540 fungal secondary metabolites with significant biological activities.
Article
Microbiology
Xuping Zhang, Xuwen Hou, Dan Xu, Mengyao Xue, Jiayin Zhang, Jiacheng Wang, Yonglin Yang, Daowan Lai, Ligang Zhou
Summary: In this study, the effects of environmental factors on U. virens were investigated. It was found that fructose, glucose, complex nitrogen sources, acidic conditions, and light exposure favored the production of sorbicillinoids. The relative transcript levels of sorbicillinoid biosynthesis genes were up-regulated when U. virens was exposed to these favorable environmental factors, indicating that sorbicillinoid biosynthesis is mainly regulated at the transcriptional level. Two pathway-specific transcription factor genes, UvSorR1 and UvSorR2, were identified to be involved in the regulation of sorbicillinoid biosynthesis. These findings provide valuable insights into the regulation mechanisms of sorbicillinoid biosynthesis and can contribute to the development of effective means for controlling sorbicillinoid production in U. virens.
Article
Biochemistry & Molecular Biology
Mengyao Xue, Siji Zhao, Gan Gu, Dan Xu, Xuping Zhang, Xuwen Hou, Jiankun Miao, Hai Dong, Dongwei Hu, Daowan Lai, Ligang Zhou
Summary: Rice false smut caused by Villosiclava virens is a highly destructive fungal disease that reduces the yield and quality of rice grains. This study isolated an albino strain LN02 from white false smut balls collected in China. The genome analysis revealed the deficiency of ustilaginoidin biosynthesis in strain LN02, which led to albinism. This finding highlights the potential of strain LN02 in the development and application of secondary metabolites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Gan Gu, Xiao Gong, Dan Xu, Yonglin Yang, Ruya Yin, Jungui Dai, Kui Zhu, Daowan Lai, Ligang Zhou
Summary: Ten new diphenyl ether polyketides, rhexocerins A-D(1-4)and rhexocercosporins A-F(5-10), as well as three known congeners (11-13), were isolated from the endophytic fungus Rhexocercosporidium sp.Dzf14 from Dioscorea zingiberensis. Their structures were determined by NMR, HRESIMS, quantum chemical ECD calculations, and X-ray crystallography. Compounds 1 and 5-9 exhibited antibacterial activities against methicillin-resistant S. aureus T144 and vancomycin-resistant E. faecalis.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Microbiology
Gan Gu, Xiaowei Jia, Weixuan Wang, Peng Li, Siji Zhao, Zhiyao Zhou, Ruya Yin, Daowan Lai, Suqin Song, Ligang Zhou
Summary: A total of 99 endophytic fungal isolates were obtained from the roots of Glycyrrhiza inflata, with 11 different genera identified, dominated by Aspergillus, Alternaria, and Fusarium. Among 22 fungi extracts, Alternaria angustiovoidea Glinf007 showed significant mycelial growth inhibitory activity against three rice fungal pathogens. The study suggests that G. inflata harbors a diverse community of endophytic fungi with potential natural antimicrobial properties beneficial for agriculture and pharmaceutical industries.
MICROBIOLOGY RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Dan Xu, Ruya Yin, Zhiyao Zhou, Gan Gu, Siji Zhao, Jin-Rong Xu, Junfeng Liu, You-Liang Peng, Daowan Lai, Ligang Zhou
Summary: Ustilaginoidins are a type of mycotoxin produced by Ustilaginoidea virens, featuring a dimeric naphtho-gamma-pyrone skeleton. The biosynthesis process involves multiple steps, including the discovery of dimeric compounds and double bond reduction. Different enzymes play roles at different stages, leading to the formation of diverse ustilaginoidins.
