Article
Materials Science, Multidisciplinary
Fanggang Liu, Zhoutong He, Yu Wang, Yongqi Zhu, Bo Huang, Alex Theodosiou, Andy Smith, Abbie Jones, Barry Marsden, Jianqiang Wang, Xingtai Zhou
Summary: Graphite is widely used in nuclear reactors as a moderator and reflector, with its pore structure and porosity evolution under ion irradiation being studied using FIB-SEM tomography. The study reveals a negative correlation between porosity and ion irradiation damage, as well as the complex morphology and closure trend of microcracks under irradiation.
JOURNAL OF NUCLEAR MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Gaku Okuma, Ryo Miyaki, Kan Shinobe, Anna Sciazko, Takaaki Shimura, Zilin Yan, Shotaro Hara, Toshinori Ogashiwa, Naoki Shikazono, Fumihiro Wakai
Summary: The study on the microstructural evolutions of Au submicron particles during free sintering and constrained sintering revealed that the anisotropy in packing structure changes with densification process and is reversed in the later stage of constrained sintering. An open pore structure model is proposed to explain the microstructural evolution in the intermediate stage.
Article
Virology
Christina Funk, Debora Marques da Silveira e Santos, Melanie Ott, Verena Raschbichler, Susanne M. Bailer
Summary: Herpes simplex virus type 1 nucleocapsids are released from the host nucleus through a process called nuclear egress, which is facilitated by two viral proteins - pUL34 and pUL31. Importantly, a functional bipartite nuclear localization signal (NLS) has been identified within pUL34, contributing to its proper subcellular localization and interaction with pUL31 for nuclear egress complex formation and viral replication.
Article
Cell Biology
Matthias Klose, Maximilian Scheungrab, Manja Luckner, Gerhard Wanner, Stefan Linder
Summary: The study used FIB-SEM tomography to visualize the interaction of Borrelia burgdorferi with human macrophages, revealing the formation of membrane tunnels and tubulations during this interaction. Additionally, it was shown that the endoplasmic reticulum forms contact sites with the membrane tunnels and phagosome tubulations, indicating its important role in the uptake and intracellular processing of Borrelia burgdorferi.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Soren Pfitzner, Jens B. Bosse, Helga Hofmann-Sieber, Felix Flomm, Rudolph Reimer, Thomas Dobner, Kay Gruenewald, Linda E. Franken
Summary: The study identified multiple destabilization phenotypes of the nuclear envelope during HAdV5 infection, including reduced lamin A/C, large-scale membrane invaginations, alterations in double membrane separation distance, and small-scale membrane protrusions. Additionally, increased nuclear membrane permeability and nuclear envelope lesions under cryoconditions were observed. Surprisingly, the ADP did not have any effect on lamin A/C reduction or nuclear permeability as previously hypothesized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Computer Science, Interdisciplinary Applications
V. Gonzalez-Ruiz, J. P. Garcia-Ortiz, M. R. Fernandez-Fernandez, J. J. Fernandez
Summary: In this study, an Optical Flow (OF)-driven interpolation strategy was developed to address the significant changes between consecutive images in FIB-SEM stacks. The approach compensated for variations by aligning the spatial regions of the biological structures using OF, resulting in sharp interpolated images. The OF-driven interpolation outperformed classical interpolation methods and produced isotropic resolution in anisotropic data.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2022)
Article
Virology
Richard J. Roller, Tineke Hassman, Alison Haugo-Crooks
Summary: The study found that substituting the HSV-1 UL34 coding sequence with VZV ORF24 resulted in viral defects in growth and spread, despite normal protein interaction and localization. Cell culture evolution showed that the mutated viruses grew and spread more efficiently, revealing the important roles of ICP22 and ICP4 in nuclear lamina disruption and capsid nuclear egress.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Jun Arii, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Yoshitaka Nakayama, Akihisa Kato, Yasuko Mori, Yasushi Kawaguchi
Summary: This study identifies an arginine cluster in the disordered domain of UL34 that interacts with ALIX and recruits ESCRT-III machinery for primary envelopment. The study highlights the importance of the disordered domain of UL34 in herpesvirus infections.
JOURNAL OF VIROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Garrett Budnik, John A. Scott, Chengge Jiao, Mostafa Maazouz, Galen Gledhill, Lan Fu, Hark Hoe Tan, Milos Toth
Summary: Nanoscale fabrication and characterization techniques are crucial for various fields, including nanoelectronics and nanobiotechnology. Focused ion beam (FIB) techniques are attractive due to their high spatial resolution and widespread use in processing nanostructured materials. In this study, we introduce FIB-induced fluorescence spectroscopy (FIB-FS) as a nanoscale technique for spectroscopic detection of ion-beam sputtered atoms. We demonstrate the nanoscale lateral and depth resolution using semiconductor heterostructures, and show that the resolution is limited by ion-induced intermixing of nanostructured materials. We also demonstrate the sensitivity of FIB-FS by depth profiling of quantum wells and detection of trace-level impurities. The utility of FIB-FS is demonstrated in the characterization of quantum wells and Li-ion batteries, combining the versatility of FIB nanofabrication techniques with the power of diffraction-unlimited fluorescence spectroscopy.
Article
Chemistry, Multidisciplinary
Daniel Garcia-Lojo, Evgeny Modin, Sergio Gomez-Grana, Marianne Imperor-Clerc, Andrey Chuvilin, Isabel Pastoriza-Santos, Jorge Perez-Juste, Doru Constantin, Cyrille Hamon
Summary: This study analyzes the organization of gold octahedra into supercrystals within microfluidic channels using X-ray scattering techniques and FIB-SEM tomography. The results show the formation of a single-domain supercrystal with long-range order, suggesting a growth mechanism directed by the channel interface. Time-resolved small angle X-ray scattering analysis reveals the accumulation of nanoparticles within the channel before nucleation and growth of the supercrystal.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
C. Fellah, T. Douillard, E. Maire, S. Meille, B. Reynard, G. Cuny
Summary: Enameloid in shark teeth is a complex structure resulting from ameloblast and odontoblast activity, forming subunits like Single Crystallite Enameloid (SCE) and Bundled Crystallite Enameloid (BCE). Research using Focused Ion Beam Nanotomography reveals a strong connection between SCE and BCE, aiding in understanding the evolution of tooth structure in sharks.
JOURNAL OF STRUCTURAL BIOLOGY
(2021)
Article
Microscopy
Jialong Liu, Suyun Niu, Guoliang Li, Zhongming Du, Yuxing Zhang, Jijin Yang
Summary: Oil and natural gas prospecting requires precise pore characterisation of insulating rock samples, which involves severe charging problems in the state-of-art FIB-SEM tomography. A new nanoprobe-assisted method was explored in FIB-SEM tomography to address this problem, where a metallic nanoprobe is induced and attached on the sample surface to create an express path for the export of excess electrons, effectively removing distortion and drift when imaging.
JOURNAL OF MICROSCOPY
(2021)
Article
Engineering, Electrical & Electronic
Johannes Hertrich, Antoine Houdard, Claudia Redenbach
Summary: This study introduces an unsupervised superresolution method based on Wasserstein patch prior, which uses a reference image to reconstruct high-resolution images with similar patch distribution, suitable for texture images or images of homogeneous material microstructures. The proposed regularizer penalizes the Wasserstein2-distance of patch distributions within the reconstruction and the reference image at different scales. The method's performance is demonstrated by applying it to two- and three-dimensional images of materials' microstructures.
IEEE TRANSACTIONS ON COMPUTATIONAL IMAGING
(2022)
Review
Chemistry, Physical
Francesco Mura, Flavio Cognigni, Matteo Ferroni, Vittorio Morandi, Marco Rossi
Summary: FIB-SEM tomography is a highly important technique for reconstructing microscopic structures in 3D with nanometer-scale resolution. This paper provides detailed steps for conducting this analysis, from experimental setup to data analysis and final reconstruction. It also highlights the versatility of the technique through a comprehensive list of applications, including batteries, shale rocks, and soft materials. The continuous technological advancements, such as the introduction of plasma and cryo-FIB models, as well as machine learning and deep learning systems, are expected to further improve resolution, data quality, automation, and efficiency, making this technique a routine tool in electron microscopy and material characterization.
Article
Engineering, Chemical
Kaitlyn P. Russell, Andrew L. Zydney, Enrique D. Gomez
Summary: This study quantitatively evaluates the 3D pore structure and virus capture behavior of two commercial virus removal filters. It finds that the PegasusTM SV4 filter, with larger pore size than 20 nm, can capture viruses throughout the filter depth, while the Viresolve (R) Pro filter can only capture viruses in very small pores near the 2 μm region near the filter exit.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Chemistry, Physical
Wei-Wei Wang, Yu Gu, Hao Yan, Kai-Xuan Li, Zhao-Bin Chen, Qi-Hui Wu, Christine Kranz, Jia-Wei Yan, Bing-Wei Mao
Summary: This study investigates the impact of solid electrolyte interphases (SEIs) formed on copper surfaces and growing lithium nuclei on the deposition and dissolution processes. The formation of SEIs on bare Cu and on surfaces of growing Li nuclei were found to have different effects on subsequent processes. Models of integrated-SEI shells and segmented-SEI shells were proposed to describe the formation of SEI shells and their impact on deposition and dissolution processes.
FARADAY DISCUSSIONS
(2022)
Article
Cell Biology
Martin Schauflinger, Tim Bergner, Gregor Neusser, Christine Kranz, Clarissa Read
Summary: High-pressure freezing followed by freeze-substitution is a valuable method for ultrastructural analyses of resin-embedded biological samples. In this study, we introduce the use of a combination of potassium permanganate, uranyl acetate, and water in acetone as complementing reagents during the freeze-substitution process. This mix provides an intense en bloc stain to cellular ultrastructure and membranes, effectively replacing osmium tetroxide.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Biology
Myriam Scherer, Clarissa Read, Gregor Neusser, Christine Kranz, Anna K. Kuderna, Regina Mueller, Florian Full, Sonja Woerz, Anna Reichel, Eva-Maria Schilling, Paul Walther, Thomas Stamminger
Summary: PML nuclear bodies undergo rearrangement to form giant cages upon viral infection, trapping viral genomes and capsids, and providing a multilayered defense strategy against viral infections.
Review
Chemistry, Physical
Eva Oswald, Krishnaveni Palanisamy, Christine Kranz
Summary: This article discusses the application of scanning electrochemical probe microscopy techniques in nanoscale 3D surface modification. The combination of electrochemically induced surface structuring allows for maskless in situ fabrication of three-dimensional nanostructures, and recent progress and practical approaches are highlighted.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Biochemical Research Methods
Giada Caniglia, Andrea Teuber, Holger Barth, Boris Mizaikoff, Christine Kranz
Summary: This study investigates the relationship between the adhesion properties of polydopamine films and their surface charge density and pH using atomic force microscopy and attenuated total reflection-Fourier transform infrared spectroscopy. The study also explores the impact of polydopamine surface charge density on bacterial adhesion and biofilm formation.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Nikhil Arya, Tom Philipp, Simon Greiner, Michael Steiner, Christine Kranz, Montaha Anjass
Summary: The reversible electrodeposition of molecular vanadium oxide clusters and the formation of thin films are reported. The reversibility is found to be dependent on the reduction potential. The multi-electron reduction of the polyoxovanadate cluster facilitates the reversible formation of potassium vanadium oxide thin films.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Andrea Teuber, Giada Caniglia, Michael Wild, Matthias Godejohann, Christine Kranz, Boris Mizaikoff
Summary: Diamond thin-film waveguides with a nanocrystalline diamond layer were used to detect the IR signature of caffeine in the mid-infrared regime. The morphological properties of the waveguides were characterized using AFM and SEM, and theoretical simulations confirmed the feasibility of using a larger sensing area compared to strip waveguides. A comparative analysis confirmed the performance of the diamond thin-film-waveguide-based sensing system, indicating its potential for various applications.
Review
Chemistry, Physical
Andreas Hellmann, Annika Schundner, Manfred Frick, Christine Kranz
Summary: ATP is a widely distributed extracellular signaling molecule that regulates various physiological and pathological processes through purinergic receptor activation. Accurate measurement of ATP release is crucial for understanding transmission mechanisms, requiring direct, sensitive, non-invasive detection with high spatial and temporal resolution. This opinion article summarizes recent research on ATP detection using electrochemical methods and provides examples of how in vitro electrochemical ATP measurements contribute to insights in purinergic signaling, specifically in alveolar homeostasis and ATP's role as a major excitatory neurotransmitter in the central nervous system.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Andrea Teuber, Giada Caniglia, Holger Barth, Christine Kranz, Boris Mizaikoff
Summary: Bacterial sensing based on quantum cascade laser spectroscopy coupled with diamond or gallium arsenide thin-film waveguides is a novel approach for obtaining high-resolution infrared spectroscopic information of bacteria. In this study, Escherichia coli was used as an example, and the laser spectroscopy was compared to conventional Fourier transform infrared spectroscopy. The proliferation behavior of E. coli on the surfaces was also investigated using atomic force microscopy and scanning electron microscopy.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Fatemehsadat Rahide, Krishnaveni Palanisamy, Jackson K. Flowers, Junjie Hao, Helge S. Stein, Christine Kranz, Helmut Ehrenberg, Sonia Dsoke
Summary: The naturally occurring amorphous Al2O3 film on an Al substrate poses a challenge for the performance of rechargeable Al batteries. This insulating Al oxide slows down electrode activation and hinders Al plating/stripping. The two sides of Al foils have different surface properties, with the non-shiny side having higher roughness and greater concentration of active sites. Various immersion pretreatments can modify the surface properties and create an interphase layer rich in Al, Cl, and N.
Article
Electrochemistry
Noha Sabi, Krishnaveni Palanisamy, Fatemehsadat Rahide, Sven Daboss, Christine Kranz, Sonia Dsoke
Summary: Investigated the impact of aluminum foil surface properties on the electrochemical behavior in aluminum battery half-cells. The purity, hardness and thickness of commercial aluminum foils influence the battery performance, with the 0.075 mm thickness showing the best cycling stability.
BATTERIES & SUPERCAPS
(2023)
Article
Electrochemistry
Krishnaveni Palanisamy, Sven Daboss, David Schaefer, Marcus Rohnke, Laurin Derr, Marcel Lang, Rolf Schuster, Christine Kranz
Summary: This study presents a cost-effective spray-coating process for the preparation of HC composite electrodes for sodium-ion batteries. The spray-coated anodes showed improved performance compared to doctor bladed anodes, indicating the potential of this method for practical applications.
BATTERIES & SUPERCAPS
(2023)
Article
Chemistry, Analytical
Andrea Teuber, Giada Caniglia, Christine Kranz, Boris Mizaikoff
Summary: Diamond thin-film waveguides combined with quantum cascade lasers augmented by drop-casted graphene enable surface-enhanced infrared absorption spectroscopy, enhancing the signal for analytical scenarios with small sample volumes and low analyte concentration levels.
Article
Chemistry, Multidisciplinary
Tatjana Weil, Abbna Kirupakaran, My-Hue Le, Philipp Rebmann, Joel Mieres-Perez, Leila Issmail, Carina Conzelmann, Janis A. Mueller, Lena Rauch, Andrea Gilg, Lukas Wettstein, Ruediger Gross, Clarissa Read, Tim Bergner, Sandra Axberg Palsson, Nadja Uhlig, Valentina Eberlein, Heike Woell, Frank-Gerrit Klaerner, Steffen Stenger, Beate M. Kuemmerer, Hendrik Streeck, Giorgio Fois, Manfred Frick, Peter Braubach, Anna-Lena Spetz, Thomas Grunwald, James Shorter, Elsa Sanchez-Garcia, Thomas Schrader, Jan Muench
Summary: This study discovered that a class of drugs called molecular tweezers can disrupt the envelope of SARS-CoV-2 and render the virus non-infectious. By modifying the molecular structure, researchers identified a series of advanced molecular tweezers that showed enhanced ability to destroy lipid bilayers and suppress SARS-CoV-2 infection. These potentiated tweezers demonstrated activity against various viruses, including respiratory syncytial virus, influenza, and measles viruses, in addition to SARS-CoV-2. The inhibitory effects of the advanced tweezers against respiratory syncytial virus and SARS-CoV-2 were also validated in mice. Therefore, these broad-spectrum antiviral agents have great potential for clinical development in combating highly pathogenic viruses.
Article
Electrochemistry
Ananyo Roy, Vinayan Bhagavathi Parambath, Thomas Diemant, Gregor Neusser, Christine Kranz, R. Juergen Behm, Zhenyou Li, Zhirong Zhao-Karger, Maximilian Fichtner
Summary: In this study, the interaction between the magnesium (Mg) metal anode and chloride (Cl)-free electrolyte was investigated. The results showed the formation of a solid interphase layer, which played a crucial role in the reversible stripping and plating of Mg in a full-cell configuration. This study provides fundamental insights into the interfacial phenomena between Mg anode and Cl-free electrolyte.
BATTERIES & SUPERCAPS
(2022)
