Prokaryotic Soluble Overexpression and Purification of Bioactive Human Growth Hormone by Fusion to Thioredoxin, Maltose Binding Protein, and Protein Disulfide Isomerase

Title
Prokaryotic Soluble Overexpression and Purification of Bioactive Human Growth Hormone by Fusion to Thioredoxin, Maltose Binding Protein, and Protein Disulfide Isomerase
Authors
Keywords
Solubility, Proteases, Disulfide bonds, Glycerol, Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Cell fusion, Growth hormone, Plasmid construction
Journal
PLoS One
Volume 9, Issue 3, Pages e89038
Publisher
Public Library of Science (PLoS)
Online
2014-03-11
DOI
10.1371/journal.pone.0089038

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