Article
Biochemical Research Methods
Shujie Wu, Songen Wang, Zongbing Li, Chun Wu, Dik-Lung Ma, Xiangmin Miao
Summary: In this work, an iridium(III) complex was synthesized and its selective interaction with a specific G-quadruplex DNA sequence was studied. Results showed that the complex exhibits high selectivity and can be used as an efficient electrochemiluminescence probe for the detection of double-stranded DNA.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zongbing Li, Seyin Zou, Shujie Wu, Xiangmin Miao, Dik-Lung Ma
Summary: A label-free fluorescent sensor using a G-quadruplex selective iridium(III) complex was developed for ultrasensitive detection of HBV DNA. The sensor showed a linear detection range of 3.0 fM to 800 pM, with a detection limit of 1.6 fM, and successfully detected HBV DNA in serum samples, demonstrating potential for biomolecular analysis.
Article
Biochemistry & Molecular Biology
Xi Zhou, Doudou Zhang, Ying Yan, Hailun He, Yukui Zhou, Changbei Ma
Summary: A label-free fluorescent method for detecting glutathione (GSH) based on a thioflavin T/G-quadruplex conformational switch is developed, showing high sensitivity and selectivity. The method can also be applied to detect other amino acids with satisfactory efficacy.
Article
Chemistry, Analytical
Shujie Wu, Seyin Zou, Songen Wang, Zongbing Li, Dik-Lung Ma, Xiangmin Miao
Summary: In this study, strong electrochemiluminescence (ECL) emission was achieved using a G-quadruplex selective iridium (III) complex as an efficient ECL signal probe. By introducing the iridium (III) complex through its specific interaction with G-quadruplex DNA modified on the surface of negatively charged gold nanoparticles ((- )AuNPs), an increased ECL signal was induced, which allowed for quantitative detection of cTnI in a range of 5.0 fg/mL100 ng/mL with a detection limit of 1.67 fg/mL. The proposed immunosensor showed great potential in the early diagnosis of acute myocardial infarction (AMI) by successfully detecting cTnI in human serums from healthy individuals and AMI patients.
Article
Chemistry, Analytical
Meijuan Du, Qian Chen, Xueqin Xu
Summary: A novel label-free electrochemical aptasensor was developed to detect metronidazole with high sensitivity and selectivity, and it was successfully applied in the detection of metronidazole in tap water and honey samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Shaokang Jia, Wenjin Wang, Shanshan Qin, Shengjie Xie, Lisi Zhan, Qi Wei, Ziang Lu, Xiaolu Zhou, Cong Chen, Kun Chen, Shen Yan, Caiping Tan, Zongwan Mao, Xiang Zhou
Summary: A phosphorescent probe Ir-PDP containing an Ir(III) complex was designed and synthesized to evaluate the level of G4 in the cytoplasm. Compared with other nucleic acid secondary structures, Ir-PDP exhibited a higher phosphorescence lifetime after interacting with G4. Two-photon phosphorescence lifetime imaging was used to detect the binding events of the probe in the cytoplasm. The addition of G4 binder PDS significantly regulated cytoplasmic phosphorescence lifetime. This project explored a new sensing pathway to observe the binding manners of probes in the cytoplasm through the phosphorescence lifetime of probes.
CHINESE CHEMICAL LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Yu Luo, Martina Lenarcic Zivkovic, Jiawei Wang, Jan Rynes, Silvie Foldynova-Trantirkova, Lukas Trantirek, Daniela Verga, Jean-Louis Mergny
Summary: Metal ions are essential for the survival of living organisms. The structures of G/C-rich sequences are influenced by the [Na+]/[K+] ratio, which can lead to the formation of competing structures.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Brondwyn S. McGhie, Jennette Sakoff, Jayne Gilbert, Christopher P. Gordon, Janice R. Aldrich-Wright
Summary: This paper describes the development of novel quadruplex DNA-stabilizing metal complexes and demonstrates their stability-enhancing effects on quadruplex DNA. Furthermore, these complexes exhibit cytotoxic effects and show good selectivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Hongjie Qi, Haiyin Li, Feng Li
Summary: Development of simple, low-cost and highly sensitive electrochemiluminescence (ECL) sensors for various targets is urgent but remains challenging. Here, an iridium (III) complex (Ir-ECL) was synthesized and applied for improved target sensing. Ir-ECL exhibited ECL emission wavelength at 590 nm and showed superior intercalation ability into G-quadruplex DNA. Using these properties, Ir-ECL was applied to enzyme-free, label-free, sensitive and homogeneous ECL analysis of pesticide acetamiprid (Ace) based on aptamer-target recognition-driven hybridization chain reaction (HCR), resulting in improved Ace ECL sensing.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Feng Chen, Guodong Li, Chun Wu, Wanhe Wang, Dik-Lung Ma, Chung-Hang Leung
Summary: In this study, a rapid and label-free interference reduction nucleic acid amplification strategy (IR-NAAS) was developed for diagnosing COVID-19 RNAs sequences. It utilized luminescent iridium(III) probes, time-resolved emission spectroscopy (TRES), and multi-branch rolling circle amplification (mbRCA) to achieve high sensitivity and specificity. The method was successfully applied to detect COVID-19 RNA sequences in various environmental and biological samples, and also showed potential for detecting other RNA viruses.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Spectroscopy
Dilan Guner, Berke Bilgenur Sener, Ceren Bayrac
Summary: Auramine o (AO) is a synthetic dye used in paper and textile industries. Although it has been banned as a food additive in many countries, it is still illegally used in certain food products and pharmaceuticals. Monitoring the presence of AO in food products is important to minimize health risks. This study developed a simple, sensitive, and selective label-free detection method based on G-quadruplex for AO. The method showed high specificity for AO and was successfully applied to detect AO in pasta, bulgur, and curry powder.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Awadesh Kumar Dwivedi, Niki Sweta Jha
Summary: We synthesized a cobalt(II) complex of a curcumin-cysteine conjugate (Co-CC) as a fluorescent probe for detecting G-quadruplex DNA sequences. The probe showed strong fluorescence emission upon adding guanine-rich DNA sequences, with high selectivity and low detection limits. It also exhibited improved cytotoxicity towards A549 lung cancer cells and mild toxicity towards Vero cells compared to pristine curcumin. Real-world sample analysis showed promising results, and this study provides insights for improving bioavailability through metal-organic ligand complexation.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Applied
Xiaomeng Guo, Dawei Yang, Ranran Sun, Qian Li, Hongyan Du, Yalin Tang, Hongxia Sun
Summary: A new fluorescence resonance energy transfer (FRET) switch was constructed that can track the progression and termination of mitophagy by identifying G-quadruplex structures. This provides a new strategy for constructing non-covalent FRET probes and offers a reliable tool for tracking mitophagy.
Article
Chemistry, Analytical
Ying Wang, Guangxu Qu, Li Yin, Qingzhi Liu, Ruimei Yang, Lihua Lu
Summary: A ratiometric luminescent detection system based on silicon nanoparticles and iridium complex has been successfully constructed for sensitively detecting transcription factor in complicated biological systems. This platform can detect low concentrations of transcription factors in buffered systems and human serum.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Valentina Pirota, Chiara Platella, Domenica Musumeci, Alessandra Benassi, Jussara Amato, Bruno Pagano, Giorgio Colombo, Mauro Freccero, Filippo Doria, Daniela Montesarchio
Summary: NDI-5 has shown promising potential as a ligand for multimeric G4 structures, selectively targeting them over duplex DNA. Experimental analyses including CD, gel electrophoresis, ITC, and fluorescence spectroscopy suggest that NDI-5 effectively binds and stabilizes tel46 G4, favoring a hybrid folding in K+-containing buffer.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Pharmacology & Pharmacy
Liqian Chen, Zhengmao Luo, Ming Wang, Jingru Cheng, Fei Li, Hanqi Lu, Qiuxing He, Yanting You, Xinghong Zhou, Hiu Yee Kwan, Xiaoshan Zhao, Lin Zhou
Summary: Chinese herbal medicines have shown promising clinical efficacies in reducing serum uric acid levels in patients with elevated levels. Clinical studies indicate that Chinese herbal medicines are effective in lowering serum uric acid levels, and experimental studies have revealed the mechanistic signaling pathways involved in this process.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Hiu Yee Kwan, Qinghua Xu, Ruihong Gong, Zhaoxiang Bian, Chih-Chang Chu
Summary: The use of folate-conjugated arginine-based poly(ester urea urethane)s nanoparticles for delivering gambogic acid (GA) shows potent anti-triple negative breast cancer (TNBC) effects with reduced off-target toxicity. This novel targeted therapeutic approach could provide a promising alternative for TNBC treatment.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Hailei Zhang, Huan Zhong, Xufeng Wang, Shoudong Zhang, Xiaojian Shao, Hao Hu, Zhiling Yu, Zongwei Cai, Xuemei Chen, Yiji Xia
Summary: New findings suggest that NAD-RNAs are involved in regulating gene expression in prokaryotes and eukaryotes through noncanonical RNA capping. The development of NAD tagSeq II using copper-free SPAAC provides a more accurate method for labeling NAD-RNAs and analyzing their profiles, revealing genome-wide alterations in E. coli during different growth phases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Minting Chen, Hsin Ling Wu, Tsz Sin Wong, Baisen Chen, Rui-Hong Gong, Hoi Leong Xavier Wong, Haitao Xiao, Zhaoxiang Bian, Hiu Yee Kwan
Summary: The combination treatment of wogonin and artesunate shows synergistic anti-HCC effect by increasing apoptosis and reducing cell viability. The treatment significantly increases the expression of GADD45A, TNF alpha, and TRAF3, leading to enhanced apoptosis and reduced HCC cell viability. The study supports further development of this combination therapy for HCC management.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Medicine, Research & Experimental
Mingjing Meng, Keying Zhong, Ting Jiang, Zhongqiu Liu, Hiu Yee Kwan, Tao Su
Summary: KRAS mutations are a major driver in CRC and significantly impact the prognosis and survival of patients. Research studies focus on exploring potential therapeutics for KRAS mutant CRC, as well as understanding the pathological consequences and treatment response of these mutations. Challenges and difficulties in treating KRAS mutant CRC are also discussed.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Cell Biology
Xianjing Hu, Sarwat Fatima, Minting Chen, Keyang Xu, Chunhua Huang, Rui-Hong Gong, Tao Su, Hoi Leong Xavier Wong, Zhaoxiang Bian, Hiu Yee Kwan
Summary: The study identified the master regulator TLR4 in CRC growth under high-fat diet, showing that palmitic acid and TLR4 play key roles in promoting cancer growth. Furthermore, palmitic acid regulates TLR4 expression in a PU.1-dependent manner, and TLR4 controls cancer metabolism to enhance CRC growth under HFD conditions.
CELL DEATH & DISEASE
(2021)
Review
Oncology
Hiu Yan Jennifer Lee, Mingjing Meng, Yulong Liu, Tao Su, Hiu Yee Kwan
Summary: This review summarizes the current findings on the therapeutic effects of herbal extracts and phytochemicals in overcoming EGFR-TKI resistance in NSCLC, as well as discusses novel molecular targets underlying EGFR-TKI resistance in NSCLC. Valuable information is provided for the development of herbal bioactive compounds as alternative treatments for EGFR-TKI-resistant NSCLC.
Review
Biochemistry & Molecular Biology
Hiu Yee Kwan, Minting Chen, Keyang Xu, Baisen Chen
Summary: This article critically reviews the impact of obesity on the exosomal cargo contents and the biogenesis of adipocyte-derived exosomes. It also discusses the differences in exosomal contents in different adipose depots, as well as the impact of obesity on exosomes derived from stromal vascular fraction components. The review also touches upon the development and potential application of exosome-based therapy for obesity and its comorbidities.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Xianjing Hu, Sarwat Fatima, Minting Chen, Tao Huang, Yuen Wa Chen, Ruihong Gong, Hoi Leong Xavier Wong, Rongmin Yu, Liyan Song, Hiu Yee Kwan, Zhaoxiang Bian
Summary: The study demonstrates that high levels of c-Myc protein in late-stage CRC can be effectively targeted by dihydroartemisinin treatment, resulting in reduced cell viability and altered metabolic phenotypes. Dihydroartemisinin shows stronger growth-inhibitory effects in late-stage CRC compared to early-stage CRC, indicating its potential as a therapeutic option for late-stage CRC. Additionally, the treatment reduces c-Myc expression, leading to downregulation of key metabolic enzymes and suppression of tumor cell proliferation.
CELL DEATH & DISEASE
(2021)
Letter
Oncology
Minting Chen, Keying Zhong, Jincheng Tan, Mingjing Meng, Chok Mei Liu, Baisen Chen, Chunhua Huang, Hoi Leong Xavier Wong, Zhaoxiang Bian, Tao Su, Hiu Yee Kwan
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Minting Chen, Fan Zhang, Baisen Chen, Condon Lau, Keyang Xu, Tiejun Tong, Chuying Huo, Quanbin Han, Tao Su, Hiu Yee Kwan
Summary: Obesity affects the cargo packaging of adipocyte-derived exosomes. Different adipose tissues have different genetic makeup, resulting in different protein profiles of exosomes under obesity conditions, which in turn influences the pathophysiological conditions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Medicinal
Shasha Cheng, Yi Kuang, Guodong Li, Jia Wu, Chung-Nga Ko, Wanhe Wang, Dik-Lung Ma, Min Ye, Chung-Hang Leung
Summary: This study reports the use of dehydroeburicoic acid (DEA) as a dual inhibitor of the Keap1-Nrf2 protein-protein interaction (PPI) and GSK3 beta, providing potential therapeutic strategies for alcoholic liver disease (ALD). DEA disrupts the Keap1-Nrf2 PPI and restores Nrf2 activity, leading to the activation of antioxidant genes. It also alleviates ethanol-induced mitochondrial dysfunction and exhibits minimal toxicity in the ALD cell model.
Review
Chemistry, Inorganic & Nuclear
Wanhe Wang, Jianhua Liu, Sang-Cuo Nao, Dik-Lung Ma, Jing Wang, Chung-Hang Leung
Summary: Changes in protein levels and types can indicate the occurrence of diseases, making differentially expressed proteins useful biomarkers for diagnosis and treatment. Luminescent iridium(III) complexes, especially affinity-based ones, with excellent optical properties, have gained attention in protein-based disease diagnostics. This review summarizes the current advances and future perspectives of affinity-based luminescent iridium(III) complexes for detecting disease-related proteins.
Article
Chemistry, Medicinal
Li Chen, Yunfei Hu, Zhonghui Lu, Zeyin Lin, Lanqing Li, Jia-Qiang Wu, Zhi-Ling Yu, Chunye Wang, Wen-Hua Chen, Jinhui Hu
Summary: Compound 7g, a dual inhibitor of tubulin polymerization and JAK2, showed potent antiproliferative activity in preclinical studies and effectively inhibited tumor growth in a xenograft model.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Meeting Abstract
Oncology
Priyanka Gupta, Kiranj Chaudagar, Sweta Sharma-Saha, Kaela Bynoe, Lea Maillat, Brian Heiss, KaHo Leung, Yamuna Krishnan, Walter Stadler, Akash Patnaik
