Article
Fisheries
Baoli Zhang, Changyue Yu, Yingkai Xu, Ziwei Huang, Yuqiao Cai, Yingdong Li
Summary: Photoperiod plays a vital role in the growth, development, and metabolism of crustaceans. The study revealed that the photoperiod affects the gene expression in the hepatopancreas of Chinese mitten crab, leading to changes in immune mechanisms. High-throughput Illumina sequencing was used to explore the genes associated with immune function, and the results demonstrated the impact of photoperiod on gene expression patterns.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Xiao-Tong Cao, Xiao-Yi Pan, Meng Sun, Yan Liu, Jiang-Feng Lan
Summary: A novel CTL (HepCL) with two CRDs identified in red swamp crayfish was found to be mainly expressed in the hepatopancreas and capable of binding to bacteria, enhancing bacterial clearance in vivo. The N-terminal CRD of HepCL showed stronger bacterial clearance activity in vivo compared to the C-terminal CRD. HepCL may be involved in the antibacterial immune response by regulating the expression of antimicrobial proteins.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hongli Liu, Yang Feng, Ma Yang, Ya Huang, Minghao Li, Yi Geng, Ping Ouyang, Defang Chen, Shiyong Yang, Lizi Yin, Liangyu Li, Xiaoli Huang
Summary: Research reveals that starvation leads to a decrease in the hepatopancreas index of Chinese mitten crab, affecting tissue structure, metabolism, and angiogenesis. The AKT signaling pathway plays a crucial role in this process, regulating gene expression related to angiogenesis and apoptosis, ultimately causing hepatopancreas atrophy.
Article
Food Science & Technology
Licheng Fan, Caining Xian, Shijie Tang, Wei Ding, Chang-Hua Xu, Xi-Chang Wang
Summary: The study found that the level of lipid oxidation in the hepatopancreas of Eriocheir sinensis varied at different temperatures, with the lowest oxidation observed at -80 degrees Celsius. A back propagation neural network with 10 neurons was effectively used to predict lipid stability during frozen storage with high accuracy. Evaluation of changes in nutritional quality using atherogenesis and thrombosis indexes proved beneficial in understanding the impact of frozen storage conditions on the samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lin Zhou, Lanlu Chu, Jinliang Du, Zhijuan Nie, Liping Cao, Jiancao Gao, Gangchun Xu
Summary: Chinese mitten crab (E. sinensis) is an important farmed crustacean in China, but farming process may lead to the occurrence of harmful factor, lipopolysaccharide (LPS). This study investigated the correlation between LPS concentrations and hepatopancreas damage in E. sinensis. Results showed that LPS significantly affected immune-related genes and antioxidant enzyme activity, resulting in hepatopancreas damage. These findings provide valuable insights into the mechanism of hepatopancreas injury induced by LPS infection in E. sinensis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2023)
Article
Environmental Sciences
Peng Huang, Jinliang Du, Liping Cao, Jiancao Gao, Quanjie Li, Yi Sun, Nailin Shao, Yuning Zhang, Gangchun Xu
Summary: In this study, the toxic effects of the triazine pesticide prometryn on Eriocheir sinensis were evaluated. The results showed that prometryn can cause damage to the hepatopancreas of E. sinensis through oxidative stress, induction of cell apoptosis, disruption of lipid metabolism, and immune damage.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Yuning Zhang, Jiancao Gao, Liping Cao, Jinliang Du, Gangchun Xu, Pao Xu
Summary: With climate warming, blue-green algae blooms have increased in frequency and severity, releasing microcystin toxins that pose potential threats to health. This study found that MC-LR activates autophagy in the hepatopancreas of Chinese mitten crabs, and identified miR-282-5p and PIK3R1 as important regulators of autophagy.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Food Science & Technology
Qiu-Ju Wang, Bao-Yuan Zhang, Xiao-Dong Jiang, Xiao-Wen Long, Wang-Ling Zhu, Yong-Ping Xu, Min Wu, Dong-Ming Zhang
Summary: The study found that adult female Chinese mitten crabs with white hepatopancreas syndrome had significantly higher moisture content in their hepatopancreas and ovaries compared to the control group, while hepatopancreas crude fat and ovary crude protein levels were significantly lower. The yellow-white hepatopancreas group had significantly higher total carbohydrate levels and significantly lower crude protein content in ovaries. Additionally, levels of certain amino acids and fatty acids were significantly altered in diseased crabs, with the white hepatopancreas group showing poor nutritional quality.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Limnology
Yi Zhang, Mengqi Ni, Jinbin Zheng, Zhaoxia Cui
Summary: This study revealed different mechanisms adopted by male and female Chinese mitten crabs in response to air exposure stress, with females showing a preference for increasing heat shock protein expression and males tending to resist stress by increasing antioxidant enzyme expression. Overall, it provides novel insights into the molecular mechanisms underlying the air exposure stress response of E. sinensis.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2022)
Article
Food Science & Technology
Long Zhang, Ning-Ping Tao, Xugan Wu, Xichang Wang
Summary: Chinese mitten crab, as an important seafood in China, is affected by the color and bitterness of its hepatopancreas, which can significantly impact consumer acceptance. This study investigated the metabolic relationship between key metabolites and the color and bitterness in the hepatopancreas of the crab. The results suggest that bile acids and bitter free amino acids may contribute to the bitterness of the hepatopancreas.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Genetics & Heredity
Yu Xu, Zhen Miao, Xuguang Li, Hai Lin, Yu Cheng, Jianlin Pan, Zhiqiang Xu
Summary: Hypoxia is a common yet overlooked issue in Chinese mitten crab rearing. This study found that the hepatopancreas transcriptome of crabs is severely affected under hypoxia, with significant changes in gene expression related to cellular adaptive responses to hypoxia. The results suggest that even after 12 hours of reoxygenation, the adverse effects of hypoxia on growth, development, and disease susceptibility in the crabs persist.
Article
Toxicology
Cheng Chi, Xia-Wei Yu, Cai-Yan Zhang, Jia-Dai Liu, Ming-Wen Ye, Ding-Dong Zhang, Wen-Bin Liu
Summary: Chinese mitten crab is economically important in aquaculture, but hepatopancreatic necrosis syndrome caused by cyanobacteria bloom outbreaks and MC-LR toxicity can affect its quality and breeding industry.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Peng Huang, Liping Cao, Jinliang Du, Jiancao Gao, Yuning Zhang, Yi Sun, Quanjie Li, Zhijuan Nie, Gangchun Xu
Summary: There is growing evidence that long-term exposure to the widely used herbicide prometryn can induce toxicity in bony fish and shrimp. This study investigated the effects of prometryn on the hepatopancreas and intestine of the crab Eriocheir sinensis through a 20-day exposure experiment. The findings suggest that prometryn can reduce antioxidant enzyme activities, increase lipid peroxidation levels, and cause oxidative stress.
Article
Environmental Sciences
Yuhang Hong, Yi Huang, Yanzhen Dong, Dayong Xu, Qiang Huang, Zhiqiu Huang
Summary: This study investigated the cytotoxic effects of abamectin on hepatopancreas cells of Chinese mitten crab. The results showed that abamectin inhibited cell viability and caused oxidative stress, DNA damage, and apoptosis. It also induced changes in antioxidant response and immune status.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Mingyu Yin, Xueyan Fu, Xichang Wang
Summary: This study investigated the changes in lipid composition of female Chinese mitten crabs using UHPLC-Q E Orbitrap mass spectrometer in a simulated thermal oxidation system. The key lipids identified were phosphatidylethanolamine (PE) and free fatty acid (FFA), with key fatty acids including C18:1, C18:3, C20:3, C20:4, C20:5, and C22:6. The degradation rate of C24:0 in FFA was found to be higher than the synthesis rate. Analysis of the molecular species provided insights into the dynamic change mechanism of lipids in the hepatopancreas, with potential implications for product processing and sensory preferences of the public.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Xingkun Jin, Ziwei Chen, Yan Shi, Jian-Fang Gui, Zhe Zhao
Summary: Environmental bacteria have a significant impact on fish gut microbiota, with feed-associated bacteria causing disturbance to the hindgut microbiota of cultured fugu. Community stability is largely influenced by homogeneous selection and dispersal limitation under low disturbance, while increased disturbance leads to variable selection and enhanced interaction within the gut microbiota, resulting in instability and shifts in the community composition.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Fisheries
Yu Song, Kaimin Zhou, Xingyu Nan, Yukai Qin, Ke Zhao, Weiwei Li, Qun Wang
Summary: The study found that ML proteins were involved in bacterial resistance in Chinese mitten crab, with one member, EsML3, being upregulated in hemocytes following bacterial challenge and binding to Gram-negative and Gram-positive bacteria. EsML3 mediates cellular immune responses by promoting bacterial clearance and phagocytosis against bacterial infection in crab.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Immunology
Hao Li, Xing-Kun Jin, Kai-Min Zhou, Hui Zhao, Yue-Hong Zhao, Qun Wang, Wei-Wei Li
Summary: A novel Dscam signaling pathway was investigated in crab, revealing its crucial functions during immune responses in invertebrates. Bacterial infection induced the cleavage of Dscam ICDs and facilitated nuclear translocation, promoting hemocyte proliferation and host protection. ADAM10 was found to play a key role in Dscam shedding activity post bacterial stimulation, mediating the cleavage of ICDs by gamma-secretase.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Environmental Sciences
Xingkun Jin, Yaofang Yang, Haihang Cao, Beile Gao, Zhe Zhao
Summary: Cobalamin, an essential micronutrient, is crucial for both prokaryotes and eukaryotes. Some Cyanobacterial and Archaeal taxa can synthesize cobalamin de novo, but the contributions of Gammaproteobacteria like the 'Psychromonadaceae' remain unknown. Uneven distribution of cobalamin biosynthetic operon and cobalamin-dependent light-responsive regulon was observed, suggesting ecological divergence impacts their metabolic pathways.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Fisheries
Hao Li, Yuehong Zhao, Xiaoli Zhang, Hui Zhao, Weiwei Li, Qun Wang
Summary: In this study, the transcriptional response of Eriocheir sinensis to different forms of Dscam ICDs was investigated. The results showed that different forms of Dscam ICDs can affect key immune, metabolic, and cell proliferation-regulated genes and pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Kaimin Zhou, Longwei Bai, Xingyu Nan, Ke Zhao, Yu Song, Weiwei Li, Qun Wang
Summary: In this study, the essential role of FADD in the IMD signaling pathway in the Chinese mitten crab Eriocheir sinensis was demonstrated. Knockdown of EsFADD led to impaired immune response and increased susceptibility to bacterial infection. The findings highlight the evolutionary conservation of FADD in crustaceans and provide insights into innate immune defense in invertebrates.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Jiying Li, Ke Zhao, Hao Li, Kaimin Zhou, Qun Wang, Weiwei Li
Summary: The study revealed that the Dscam gene in the Chinese mitten crab has different variants that play a role in immune regulation, with significant increase in expression levels after bacterial stimulation. The two variants also differ in their regulation of antimicrobial peptides, proportion of carried exons, and bacterial phagocytosis.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hui Zhao, Zhe Chen, Hao Li, Yue-Hong Zhao, Qun Wang, Wei-Wei Li
Summary: This study demonstrates that bacteria can induce hemocyte proliferation in Chinese mitten crabs, and EsCsn5 plays a negative regulatory role in this process by controlling the degradation of EsCyclin E. Knockdown of EsCsn5 enhances hemocyte proliferation and reduces survival rate in crabs, leading to increased bacterial concentration in the hemolymph.
Article
Immunology
Kaimin Zhou, Yukai Qin, Yu Song, Ke Zhao, Weijuan Pan, Xingyu Nan, Yue Wang, Qun Wang, Weiwei Li
Summary: The study reveals the antibacterial role of a member of the C-type lectin family, EsIgLectin, in the Chinese mitten crab. EsIgLectin is expressed in hemocytes and intestines, and it plays a critical role in bacterial binding, agglutination, growth inhibition, and clearance. It promotes bacterial phagocytosis in hemocytes and protects against inflammation in the intestines, functioning in a polymeric Ig receptor-dependent manner.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Microbiology
Xixi Li, Ce Zhang, Xingkun Jin, Fucheng Wei, Fei Yu, Douglas R. Call, Zhe Zhao
Summary: Phage HH109 was found to exhibit control over host genes during infection, inducing rapid and distinctive changes. Gene co-expression analysis predicted that the late gene gp27 encoded by HH109 modulates the host's membrane transport and/or transcriptional regulation. Additionally, bacterial virulence genes were downregulated while drug resistance genes were upregulated.
Article
Fisheries
Yue Wang, Xingyu Nan, Shuqi Hao, Ke Zhao, Yanan Guo, Qun Wang, Weiwei Li
Summary: AKT plays an important role in regulating cell metabolism and cell proliferation in Eriocheir sinensis. The EsAkt protein is highly conserved across species, and its expression is up-regulated in hemocytes after stimulation with gram-positive or gram-negative bacteria. Knockout of the EsAkt gene reduces cell glucose metabolism and inhibits cell proliferation.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Editorial Material
Physiology
Xingkun Jin, Xiaodan Wang, William Ka Fai Tse, Yan Shi
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Fisheries
Zi-Wei Chen, Xing-Kun Jin, Fan-Xiang Gao, Jian-Fang Gui, Zhe Zhao, Yan Shi
Summary: The structure and function of gut microbiota in fish can be influenced by fish sex, but the impact is limited, mainly affecting certain bacterial taxa. The relative abundance of sex-specific bacterial taxa plays a role in determining bacterial phenotypes.
Article
Environmental Sciences
Xingyu Nan, Xingkun Jin, Yu Song, Kaimin Zhou, Yukai Qin, Qun Wang, Weiwei Li
Summary: Using Chinese mitten crabs as a model, this study investigated the effects of polystyrene nanoplastics (PS NPs) on cell apoptosis, glucose metabolism, and antibacterial immunity. The results showed that PS NPs were endocytosed by crab hemocytes, leading to abnormal cell apoptosis, glucose metabolism disorder, and changes in antimicrobial immunity.
ENVIRONMENTAL POLLUTION
(2022)
Article
Microbiology
Xingkun Jin, Haoyong Zhu, Yan Shi, Ziwei Chen, Yaohui Wang, Jian-Fang Gui, Zhe Zhao
Summary: Through gut microbiota profiling, we found that hybridization and cohabitation of pufferfish can significantly disturb the gut microbiota composition and function. These results enhance our understanding of the complex and dynamic assemblage of the fish holobiont from a microbial perspective and have practical implications for optimizing performance prediction and improving farmed fish production.
