Article
Cell Biology
Charles Banliat, Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Valerie Labas, Benoit Guyonnet, Pascal Mermillod, Marie Saint-Dizier
Summary: A comprehensive study of proteome dynamics in non-rodent mammalian embryos developed in vivo was conducted. Proteomic changes in bovine embryos at different developmental stages were analyzed, and distinct patterns were observed. Proteins that increased in abundance during development were associated with RNA metabolism, protein translation, and ribosome biogenesis, while proteins that decreased in abundance were involved in Golgi vesicle transport and protein processing in endoplasmic reticulum. The results provide valuable insights into the molecular mechanisms underlying early embryo development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Obstetrics & Gynecology
Aisling Ahlstrom, Kersti Lundin, Anna-Karin Lind, Kristina Gunnarsson, Goran Westlander, Hannah Park, Anna Thurin-Kjellberg, Steinunn A. Thorsteinsdottir, Snorri Einarsson, Mari Astrom, Kristina Lofdahl, Judith Menezes, Susanne Callender, Cina Nyberg, Jens Winerdal, Camilla Stenfelt, Brit-Randi Jonassen, Nan Oldereid, Lisa Nolte, Malin Sundler, Thorir Hardarson
Summary: The use of a commercially available time-lapse algorithm for Day 5 blastocyst selection did not improve ongoing pregnancy rates compared with morphology alone.
HUMAN REPRODUCTION
(2022)
Article
Engineering, Biomedical
Weihai Xu, Yiqi Yu, Shishi Li
Summary: This study investigated whether repeating laser-assisted hatching (LAH) procedure on day 6 low-grade cleavage-stage embryos (LGCEs) helps blastulation. The results showed that compared to the group that only underwent LAH on day 4, the group that underwent a repeat LAH procedure on day 6 had higher day 7 blastocyst formation rate and usable blastocyst rate.
LASERS IN MEDICAL SCIENCE
(2023)
Article
Veterinary Sciences
Alejandro Gonzalez-Plaza, Josep M. Cambra, Inmaculada Parrilla, Maria A. Gil, Emilio A. Martinez, Cristina A. Martinez, Cristina Cuello
Summary: The study found that the open Cryotop (R) system is suitable for simultaneous vitrification of 20 porcine embryos at different developmental stages. The viability and quality results of embryos vitrified with this system were comparable to those of fresh embryos subjected to 24 hours of in vitro culture.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Charles Banliat, Coline Mahe, Regis Lavigne, Emmanuelle Com, Charles Pineau, Valerie Labas, Benoit Guyonnet, Pascal Mermillod, Marie Saint-Dizier
Summary: The study revealed significant differences in protein expression between in vivo and in vitro developed bovine embryos, with embryos developed in vivo internalizing large amounts of oviductin and other proteins early in development, potentially contributing to their higher developmental ability.
Article
Obstetrics & Gynecology
Firat Tulek, Alper Kahraman
Summary: This study evaluated and compared the outcomes of fresh day 5 morula transfers and fresh day 6 blastocyst transfers after extended culture in women with a whole cohort of slow developing embryos. The results showed that although the implantation rate of day 6 blastocyst transfers was significantly higher, the clinical pregnancy and live birth rates were similar between the two groups. Therefore, for women with only slow developing embryos, there is no significant difference in the success rates between fresh day 5 morula transfers and day 6 blastocyst transfers after extended culture.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2022)
Article
Obstetrics & Gynecology
Sydney L. Lane, Laura Reed, William B. Schoolcraft, Mandy G. Katz-Jaffe
Summary: This study investigated the reproductive potential of embryos that undergo preimplantation genetic testing for aneuploidies (PGT-A) in infertility patients with slow embryo development. The study found that day 7 embryos resulted in lower implantation and live birth rates compared to day 5 and day 6 embryos. Day 7 embryos with expansion grade 5 or 6 and trophectoderm grade A were more likely to be euploid. These findings can help manage patient expectations in cases of slow embryo development.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Endocrinology & Metabolism
Junlan Yang, Ze Wang, Hairu Cao, Lu Liu, Qiaona Yuan, Haiyan Xu, Rong Tang
Summary: This study suggests that delayed blastocyst transfer on day 6 compared with day 5 may adversely affect the pregnancy rate, but it does not affect neonatal birth weight.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Obstetrics & Gynecology
Yanh Liu, Kee Ong, Irving Korman, Ross Turner, David Shaker, Deirdre Zander-Fox, Luk Rombauts
Summary: This study found that the timing of assessment on day 5 is a confounding factor in predicting live birth using snapshot blastocyst morphology. A numerical algorithm incorporating day 5 assessment HPI, blastocyst morphology, and maternal age can be developed for live birth prediction.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Endocrinology & Metabolism
Ningling Wang, Xinxi Zhao, Meng Ma, Qianqian Zhu
Summary: The study found that there was a significant increase in live birth rate and birth weight after transfer of single good quality embryo on day 5 and day 6 compared with transfer of single good quality embryo on day 3 in vitrified embryo transfer cycles.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Obstetrics & Gynecology
Jie Wang, Zhenyu Diao, Junshun Fang, Lihua Zhu, Zhipeng Xu, Fei Lin, Ningyuan Zhang, Linjun Chen
Summary: This retrospective study suggests that the day 3 embryo cell number has an influence on the clinical pregnancy and live birth rates of day 5 single blastocyst transfer in frozen embryo transfer (FET) cycles. As the day 3 embryo cell number increases, the clinical pregnancy and live birth rates also increase, especially when fair- and poor-quality blastocysts are transferred.
BMC PREGNANCY AND CHILDBIRTH
(2022)
Article
Obstetrics & Gynecology
Tiffany Stankewicz, Maria Ruiz-Alonso, Marc Soler-Ibanez, Carlos Simon, Diana Valbuena
Summary: The study found that there is no difference in clinical outcomes between day-5 and day-6 blastocysts when transferred during the optimal endometrial receptivity as determined by ERA. This suggests that both day-5 and day-6 blastocysts have similar clinical potential.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Biochemistry & Molecular Biology
Ko-Tung Chang, Yu-Ting Su, Yi-Ru Tsai, Kuo-Chung Lan, Yan-Der Hsuuw, Hong-Yo Kang, Wen-Hsiung Chan, Fu-Jen Huang
Summary: High levels of estradiol directly impact blastocysts during implantation, impairing their implantation and early development.
BIOMEDICAL JOURNAL
(2022)
Article
Genetics & Heredity
Roni Rahav-Koren, Shmuel Inbar, Netanella Miller, Amir Wiser, Yael Yagur, Chen Berkowitz, Sivan Farladansky-Gershnabel, Adrian Shulman, Arie Berkowitz
Summary: The study suggests that culturing post-thaw cleavage embryos for 2 days and transferring them as blastocysts may increase the chances of ongoing pregnancy and delivery compared to transferring thawed blastocysts directly.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Hyun Jin Kim, Jae Kyun Park, Jin Hee Eum, Haengseok Song, Woo Sik Lee, Sang Woo Lyu
Summary: Selecting a high-quality embryo is crucial for improving pregnancy outcomes, with blastocoel expansion and inner cell mass grade being important predictors for ongoing pregnancy. Embryos developing into blastocysts on day 5 and having higher expansion grades in pre-vitrified blastocysts are associated with successful pregnancy outcomes in single vitrified-warmed blastocyst transfer cycles.
REPRODUCTIVE SCIENCES
(2021)
Article
Cell Biology
Gemma Fabozzi, Emilia Rega, Maria Flavia Starita, Maria Giulia Amendola, Antonio Colicchia, Pierluigi Giannini, Claudio Piscitelli
Article
Obstetrics & Gynecology
Roberta Maggiulli, Danilo Cimadomo, Gemma Fabozzi, Letizia Papini, Lisa Dovere, Filippo Maria Ubaldi, Laura Rienzi
HUMAN REPRODUCTION
(2020)
Article
Obstetrics & Gynecology
Gemma Fabozzi, Danilo Cimadomo, Roberta Maggiulli, Alberto Vaiarelli, Filippo Maria Ubaldi, Laura Rienzi
FERTILITY AND STERILITY
(2020)
Article
Obstetrics & Gynecology
Roberta Maggiulli, Adriano Giancani, Gemma Fabozzi, Lisa Dovere, Luisa Tacconi, Maria Giulia Amendola, Danilo Cimadomo, Filippo Maria Ubaldi, Laura Rienzi
REPRODUCTIVE BIOMEDICINE ONLINE
(2020)
Article
Environmental Sciences
Donatella Paoli, Francesco Pallotti, Anna Pia Dima, Elena Albani, Carlo Alviggi, Franco Causio, Carola Conca Dioguardi, Alessandro Conforti, Rosanna Ciriminna, Gemma Fabozzi, Giuseppe Giuffrida, Roberto Gualtieri, Maria Giulia Minasi, Simona Ochetti, Valerio Pisaturo, Cinzia Racca, Laura Rienzi, Elena Sarcina, Catello Scarica, Giovanna Tomasi, Cristina Verlengia, Rita Villeggia, Federica Zullo, Andrea Lenzi, Francesco Botre, Lucia De Santis
Review
Biochemistry & Molecular Biology
Gemma Fabozzi, Benedetta Iussig, Danilo Cimadomo, Alberto Vaiarelli, Roberta Maggiulli, Nicolo Ubaldi, Filippo Maria Ubaldi, Laura Rienzi
Summary: Nutrition plays a crucial role in reproduction, especially during the peri-conceptional period, with a healthy dietary pattern and nutrient supplementation being important for optimal fetal development. Mitochondria are key elements in oogenesis and embryo development, and imbalanced dietary intakes can lead to adverse effects on their function, impacting reproductive outcomes.
Article
Genetics & Heredity
Danilo Cimadomo, Alberto Vaiarelli, Cecilia Petriglia, Gemma Fabozzi, Susanna Ferrero, Mauro Schimberni, Cindy Argento, Silvia Colamaria, Maddalena Giuliani, Nicolo Ubaldi, Laura Rienzi, Filippo Maria Ubaldi
Summary: The study found that using GnRH-a or u-hCG ovulation triggers in vitrified-warmed euploid blastocyst transfer did not significantly affect oocyte maturity and implantation rates. However, there was an impact on the overall live birth rate due to factors such as patient age and number of inseminated oocytes.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Gemma Fabozzi, Laura Albricci, Danilo Cimadomo, Maria Giulia Amendola, Federica Sanges, Roberta Maggiulli, Filippo Maria Ubaldi, Laura Rienzi
Summary: A study on sibling oocytes from women aged 42 years or younger undergoing ICSI cycles with ejaculated spermatozoa compared blastulation rates of a novel Geri-medium and a continuous single culture medium (CSCM), showing similar rates but differences in blastocyst morphology and developmental timings. Euploidy and ongoing implantation rates were similar between the two media, suggesting further research on the observed differences is needed.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Obstetrics & Gynecology
Gemma Fabozzi, Danilo Cimadomo, Mariachiara Allori, Alberto Vaiarelli, Silvia Colamaria, Cindy Argento, Maria Giulia Amendola, Federica Innocenti, Daria Soscia, Roberta Maggiulli, Rossella Mazzilli, Nicole Ubaldi, Benedetta Iussig, Marco Marchetti, Laura Rienzi, Filippo Maria Ubaldi
Summary: This study found that maternal BMI has a moderate impact on blastocyst euploidy rate and live birth rate. Clinical outcomes were similar between underweight and normal weight women, while overweight women showed higher miscarriage rate and lower live birth rate.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Multidisciplinary Sciences
Roberta Maggiulli, Alberto Vaiarelli, Danilo Cimadomo, Adriano Giancani, Luisa Tacconi, Gemma Fabozzi, Filippo Maria Ubaldi, Laura Rienzi
Summary: Oocyte cryopreservation is recognized as the gold standard for fertility preservation in women, particularly in medical cases such as oncologic diseases, severe endometriosis, systemic diseases, and genetic conditions involving premature menopause. This method focuses on maximizing the ovarian reserve and retrieving the maximum number of oocytes in the shortest time possible, with detailed evaluation and procedures outlined for efficacy, efficiency, and safety.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Genetics & Heredity
S. Canosa, D. Cimadomo, A. Conforti, R. Maggiulli, A. Giancani, A. Tallarita, F. Golia, G. Fabozzi, A. Vaiarelli, G. Gennarelli, A. Revelli, F. Bongioanni, C. Alviggi, F. M. Ubaldi, L. Rienzi
Summary: This systematic review and meta-analysis examined the impact of prolonged cryo-storage on embryo competence and perinatal outcomes. The results indicate that cryo-storage duration beyond 12 months does not significantly affect survival rate, miscarriage, live birth, and major malformation rates.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Obstetrics & Gynecology
Danilo Cimadomo, Gemma Fabozzi, Lisa Dovere, Roberta Maggiulli, Laura Albricci, Federica Innocenti, Daria Soscia, Adriano Giancani, Alberto Vaiarelli, Maurizio Guido, Filippo Maria Ubaldi, Laura Rienzi
Summary: The study aimed to evaluate the impact of cryo-storage duration on clinical, obstetric and perinatal outcomes after vitrified-warmed euploid blastocyst transfer. It concluded that cryo-storage duration does not affect these outcomes.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Review
Cell Biology
Paola Rebuzzini, Gemma Fabozzi, Danilo Cimadomo, Filippo Maria Ubaldi, Laura Rienzi, Maurizio Zuccotti, Silvia Garagna
Summary: Environmental toxicants can have multigenerational effects on reproductive systems, impacting both exposed and unexposed individuals. Epigenetic mechanisms may play a crucial role in the non-genetic transmission of environmental information.
Article
Cell Biology
Gemma Fabozzi, Paola Rebuzzini, Danilo Cimadomo, Mariachiara Allori, Marica Franzago, Liborio Stuppia, Silvia Garagna, Filippo Maria Ubaldi, Maurizio Zuccotti, Laura Rienzi
Summary: The gut microbiota plays an important role in both male and female fertility, and dysbiosis of the gut microbiota may contribute to reproductive issues. Exposure to endocrine-disrupting chemicals is a major cause of gut microbiota dysbiosis and can have significant effects on host health and potential transgenerational effects.
Article
Obstetrics & Gynecology
Danilo Cimadomo, Laura Sosa Fernandez, Daria Soscia, Gemma Fabozzi, Francesca Benini, Amalia Cesana, Maria Beatrice Dal Canto, Roberta Maggiulli, Saverio Muzzi, Catello Scarica, Laura Rienzi, Lucia De Santis
Summary: Intra-centre reliability of embryo grading performed according to the Istanbul Consensus was generally moderate/substantial, while inter-centre reliability was fair. Interactive training improved inter-centre reliability to a moderate level. The use of external quality assessment services and artificial intelligence could further enhance the reliability of embryo selection.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)