Review
Veterinary Sciences
Mehdi Soltani, Bernardo Baldisserotto, Seyed Pezhman Hosseini Shekarabi, Shafigh Shafiei, Masoumeh Bashiri
Summary: Although medicinal herbs and plants do not show strong in vitro antagonism and in vivo disease resistance towards lactococcosis agents in aquaculture, essential oils containing thymol or carvacrol have been found to be more effective. In vivo studies have shown moderate efficacy of extracts in reducing mortality in infected animals and improving immune parameters. Further research is required to fully understand the potential of medicinal herbs in combating lactococcosis.
VETERINARY SCIENCES
(2021)
Article
Crystallography
Hackwon Do, Ying Wang, Chang Woo Lee, Wanki Yoo, Sangeun Jeon, Jisub Hwang, Min Ju Lee, Kyeong Kyu Kim, Han-Woo Kim, Jun Hyuck Lee, T. Doohun Kim
Summary: In this study, a gene encoding LgEstI was cloned from a bacterial fish pathogen, Lactococcus garvieae. The enzymatic activity and crystal structure of LgEstI were analyzed. The results showed that LgEstI has high esterase activity and the crystal structure of LgEstI was successfully determined. These findings contribute to the understanding of the function of LgEstI and provide a basis for protein engineering studies.
Article
Fisheries
Ilhan Altinok, Rafet Cagri Ozturk, Mustafa Ture
Summary: The whole genome sequence of Lactococcus garvieae Lg-per, originally isolated from rainbow trout in Turkey, was analyzed. The study revealed that Lg-per was actually Lactococcus petauri, not L. garvieae as previously believed. The 16S rRNA-based PCR detection method was found to be inadequate for identifying Lactococcus strains. This is the first report of L. petauri in Turkey and highlights the importance of using whole genome sequencing for analyzing L. garvieae isolates in other countries.
JOURNAL OF FISH DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Min Tang, Hui Zhang, Yao Wan, Ziqiu Deng, Xuemei Qin, Rongbai Li, Fang Liu
Summary: Bacterial leaf streak (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a major quarantine disease. Breeding disease-resistant varieties is crucial for prevention and treatment. In this study, the molecular mechanism of resistance was investigated by analyzing the response of a BLS-resistant near isogenic line to infection by different pathogenic strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Javier Feito, Carlos Araujo, Sara Arbulu, Diogo Contente, Beatriz Gomez-Sala, Lara Diaz-Formoso, Estefania Munoz-Atienza, Juan Borrero, Luis M. Cintas, Pablo E. Hernandez
Summary: This study reported the design of Lactococcus lactis strains that can produce bacteriocins GarA and/or GarQ, along with NisA or NisZ. The strains with GarA, GarQ, and NisZ demonstrated the highest antimicrobial activity against virulent L. garvieae strains.
Article
Biotechnology & Applied Microbiology
Qian Liu, Di Luo, Mengjia Wang, Xingmin Song, Xiaofang Ye, Roman Jashenko, Rong Ji
Summary: This study conducted transcriptome analysis of C. italicus eggs treated at different temperatures, revealing differential expression patterns of cold tolerance-related genes and identifying important gene pathways involved in cold tolerance. Additionally, this study verified four potential RNAi target genes in C. italicus eggs and provided new insights for effective control of this species.
Article
Biotechnology & Applied Microbiology
Chengchao Yang, Xiaoyu Li, Yan Zhang, Hua Jin
Summary: This study identified genes related to cold adaptation and freeze-thaw injury repair in poplar trees, which could be valuable for breeding cold-tolerant varieties.
Article
Fisheries
Renata Catao Egger, Julio Cesar Camara Rosa, Luis Fernando Lara Resende, Santiago Benites de Padua, Fernanda de Oliveira Barbosa, Mariana Taise Zerbini, Guilherme Campos Tavares, Henrique Cesar Pereira Figueiredo
Summary: This study investigated lactococcosis outbreaks in Nile tilapia on Brazilian farms and characterized the isolates through multilocus sequence typing (MLST), virulence to Nile tilapia, and antimicrobial susceptibility. The pathogenicity of L. petauri to Nile tilapia was confirmed, and its rapid expansion was observed. Provisional epidemiological cutoff values were determined for L. petauri for six antimicrobial agents from different drug classes.
Article
Microbiology
Juliet F. Nilsson, Lucas G. Castellani, Walter O. Draghi, Ezequiel G. Mogro, Daniel Wibberg, Anika Winkler, L. H. Hansen, Andreas Schluter, Alfred Puhler, Jorn Kalinowski, Gonzalo A. Torres Tejerizo, Mariano Pistorio
Summary: Acidic environments and improper agricultural management can lead to soil acidification globally, impacting the interaction between plants and rhizobia. Research on Rhizobium favelukesii revealed that under acidic conditions, 60% of genes were underexpressed. Transcriptome sequencing identified mechanisms including changes in energy metabolism and protein turnover, as well as GABA and histidine metabolism, contributing to acid tolerance in the bacteria.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nan Jia, Yuting Jiang, Xianyi Jian, Tong Cai, Qing Liu, Yuan Liu, Dan Xing, Yande Dong, Xiaoxia Guo, Tongyan Zhao
Summary: In this study, the vector competence of Aedes albopictus mosquitoes from Jinghong and Guangzhou Cities of China for Zika virus (ZIKV) was evaluated. The results showed that both mosquito strains were susceptible to ZIKV, but the Guangzhou strain showed higher competence. The differential gene expression in response to ZIKV infection was different between tissues and strains, and cytochrome P450 304a1 (CYP304a1) was identified as a potentially important gene in vector competence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Defeng Zhuang, Ce Ma, Li Xue, Zhen Li, Cheng Wang, Jiajun Lei, Xingfu Yuan
Summary: This study investigated the metabolic response of Rosa xanthina f. spontanea under cold stress at the transcriptome level. A total of 124,106 unigenes were generated through de novo assembly, revealing differentially expressed genes and enriched pathways related to cold stress. The findings provide insights into the molecular mechanisms of cold resistance in rose and offer potential candidate genes for enhancing cold stress tolerance.
Article
Environmental Sciences
Prabin Dawadi, Ranjeeta Odari, Ram Chandra Poudel, Lok R. Pokhrel, Lok Ranjan Bhatt
Summary: This study detected and identified L. garvieae NEP21 for the first time in raw cow milk samples from a smallholder farm in Nepal. The prevalence of this pathogen in raw cow milk suggests the need for further research and surveillance to understand its presence in Nepal and globally, in order to better manage its infection in cattle and humans.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Genetics & Heredity
Carlos Frances-Cuesta, Ivan Ansari, Jose Francisco Fernandez-Garayzabal, Alicia Gibello, Fernando Gonzalez-Candelas
Summary: Lactococcus garvieae is a well-known pathogen in fish and rarely causes infections in humans and other mammals. This study presents the complete genomic sequence of a clinical L. garvieae strain and compares it with other genomes. The results show that L. garvieae is a highly recombinogenic species with horizontal gene transfers comprising nearly 30% of its genome.
MICROBIAL GENOMICS
(2022)
Article
Plant Sciences
Zhaoran Wei, Qiaoling Yuan, Hai Lin, Xiaoxia Li, Chao Zhang, Hongsheng Gao, Bin Zhang, Huiying He, Tianjiao Liu, Zhang Jie, Xu Gao, Shandang Shi, Bo Wang, Zhenyu Gao, Lingrang Kong, Qian Qian, Lianguang Shang
Summary: The study observed changes in three main biomass traits in rice seedlings after high-temperature treatment, and identified relevant loci and important candidate genes associated with the rice response to abnormal high temperatures. These findings provide valuable insights for further exploration of beneficial heat-resistant genes for rice breeding.
Article
Immunology
Feng-Jie Su, Meei-Mei Chen
Summary: The study demonstrates that the biofilm vaccine produced using bacteria grown on chitosan particles significantly enhances immune response in fish, providing effective prevention and treatment for L. garvieae infection, surpassing traditional whole-cell vaccines.
Article
Genetics & Heredity
Carlos Frances-Cuesta, Ivan Ansari, Jose Francisco Fernandez-Garayzabal, Alicia Gibello, Fernando Gonzalez-Candelas
Summary: Lactococcus garvieae is a well-known pathogen in fish and rarely causes infections in humans and other mammals. This study presents the complete genomic sequence of a clinical L. garvieae strain and compares it with other genomes. The results show that L. garvieae is a highly recombinogenic species with horizontal gene transfers comprising nearly 30% of its genome.
MICROBIAL GENOMICS
(2022)
Article
Biochemistry & Molecular Biology
Antonio Maldonado-Barragan, Estibaliz Alegria-Carrasco, Maria del Mar Blanco, Ana Isabel Vela, Jose Francisco Fernandez-Garayzabal, Juan Miguel Rodriguez, Alicia Gibello
Summary: Lactococcus garvieae is an emerging pathogen that causes infectious diseases in animals and potentially in humans. In this study, two gene clusters encoding novel bacteriocins were identified in a plasmid of a clinical isolate. These bacteriocins exhibited inhibitory activity against L. garvieae strains and other bacteria, and they may use a mannose uptake system as a receptor. Interestingly, they were highly active against their own host.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Karim Dib, Amal El Banna, Clara Radulescu, Guillermo Lopez Campos, Gerard Sheehan, Kevin Kavanagh
Summary: Histamine has different effects on neutrophil capture of bacteria and bacterial intracellular killing, mediated by H4R and H2R pathways respectively. Additionally, histamine can interfere with neutrophil phagocytosis by maintaining histamine levels.
JOURNAL OF INNATE IMMUNITY
(2023)
Article
Microbiology
Ricardo Calderon-Gonzalez, Alix Lee, Guillermo Lopez-Campos, Steven J. Hancock, Joana Sa-Pessoa, Amy Dumigan, Ronan McMullan, Eric L. Campbell, Jose A. Bengoechea
Summary: Klebsiella pneumoniae is a significant cause of infections, especially antibiotic-resistant ones. It colonizes the nasopharynx and gastrointestinal tract without symptoms, but colonization of the gut is essential for infection. A new murine model has been developed to study gastrointestinal colonization without tissue dissemination, providing insights into the disease dynamics of K. pneumoniae and its colonization resistance.
Article
Agriculture, Dairy & Animal Science
Johan Manuel Calderon Bernal, Ana Fernandez, Jose Luis Arnal, Celia Sanz Tejero, Jose Francisco Fernandez-Garayzabal, Ana Vela, Dolores Cid
Summary: This study characterized 170 P. multocida isolates from 125 BRD outbreaks using different typing approaches. The results revealed a very low genetic diversity among P. multocida, with most isolates belonging to the A:L3 genotype. Furthermore, the results of MLST and PFGE supported the clonal population structure of BRD-associated P. multocida isolates. These findings highlight the genetic relatedness of most P. multocida isolates associated with BRD in cattle.
Article
Computer Science, Information Systems
Kerstin Denecke, Octavio Rivera Romero, Carolyn Petersen, Marge Benham-Hutchins, Miguel Cabrer, Shauna Davies, Rebecca Grainger, Rada Hussein, Guillermo Lopez-Campos, Fernando Martin-Sanchez, Mollie McKillop, Mark Merolli, Talya Miron-Shatz, Jesus Daniel Trigo, Graham Wright, Rolf Wynn, Carol Hullin (Lucay Cossio), Elia Gabarron
Summary: This article proposes a preliminary definition of participatory health informatics (PHI) and defines the scope of the field through an adapted Delphi study design. Consensus was reached on 16 characteristics, 9 aims, and 6 related fields of PHI through three survey rounds and expert consensus.
METHODS OF INFORMATION IN MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Julio Alvarez, Johan M. Calderon M. Bernal, Laura Torre-Fuentes, Marta Hernandez, Chris E. Pinto Jimenez, Lucas Dominguez, Jose F. Fernandez-Garayzabal, Ana I. Vela, Dolores Cid
Summary: This study investigates the antimicrobial resistance levels and genetic resistance mechanisms of M. haemolytica isolated from clinically healthy sheep at slaughter. The results show that most antimicrobials have low levels of resistance except for tetracycline and tylosin. However, several resistance genes, some of which are associated with plasmids, are found in the genome of some isolates, indicating the need for further attention.
Article
Microbiology
Nadia Stoppani, Silvia Colussi, Paolo Pastorino, Marino Prearo, Simona Sciuto, Ilhan Altinok, Rafet Cagri Ozturk, Mustafa Ture, Ana Isabel Vela, Maria del Mar Blanco, Charalampos Kotzamanidis, Konstantina Bitchava, Andigoni Malousi, Lucio Fariano, Donatella Volpatti, Pier Luigi Acutis, Jose Francisco Fernandez-Garayzabal
Summary: The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri for accurate discrimination between these two species. Seven SNPs were identified that separate L. garvieae from L. petauri based on the ITS region sequence. The 16S-23S rRNA ITS region can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.
Article
Fisheries
Sarker Mohammed Ibrahim Khalil, Elena Sacca, Marco Galeotti, Simona Sciuto, Nadia Stoppani, Pier Luigi Acutis, Rafet Cagri Ozturk, Konstantina Bitchava, Maria Del Mar Blanco, Lucio Fariano, Marino Prearo, Silvia Colussi, Donatella Volpatti
Summary: Italian rainbow trout farms often experience outbreaks of lactococcosis caused by Lactococcus garvieae, with both asymptomatic and symptomatic fish present in rearing basins. This study aimed to examine immune-related gene expression and investigate Near Infrared Spectroscopy (NIR) as a tool for distinguishing between infected and healthy fish. The results showed significant differences in gene expression between symptomatic and asymptomatic fish, particularly for cytokines IL-1 beta and IL-8. NIR scanning successfully differentiated between the two fish groups. This study also demonstrated the importance of specific gene expression patterns in the trout immune system's response to L. garvieae infection.
Article
Biochemistry & Molecular Biology
Raquel Coronel, Adela Bernabeu-Zornoza, Charlotte Palmer, Rosa Gonzalez-Sastre, Andreea Rosca, Patricia Mateos-Martinez, Victoria Lopez-Alonso, Isabel Liste
Summary: This study investigates the effects of amyloid precursor protein (APP) overexpression on human neural stem cells (hNSCs) and identifies differentially expressed genes related to neuronal and glial differentiation processes, as well as signaling pathways such as Notch, Wnt, PI3K-AKT, and JAK-STAT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Raquel Coronel, Victoria Lopez-Alonso, Marta I. Gallego, Isabel Liste
Summary: This article investigates the biological role of APP in hNSCs cells, revealing its association with cell proliferation and cell fate specification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Johan Manuel Calderon Bernal, Carlos Serna, Angel Munoz, Alberto Diez Guerrier, Lucas Dominguez, Jose Francisco Fernandez-Garayzabal, Ana Isabel Vela, Dolores Cid
Summary: The aim of this study was to investigate the possible genotypic differences between commensal Pasteurella multocida isolates from apparently healthy animals and those from animals affected by respiratory diseases. The study found that nearly all isolates from clinical cases were significantly associated with respiratory diseases. In addition, ST79 isolates carried resistance genes to commonly used antimicrobial drugs for treating respiratory diseases.
Proceedings Paper
Computer Science, Interdisciplinary Applications
Christopher Hawthorne, Donal Harrison, Eva McEvoy, Guillermo Lopez Campos
Summary: This work investigates the genes, diseases, and molecular mechanisms associated with exposure to different subsets of electromagnetic fields. The results reveal a significant number of unique disease terms and genes related to electromagnetic field exposure, highlighting the molecular aspects of this increasing exposure.
CARING IS SHARING-EXPLOITING THE VALUE IN DATA FOR HEALTH AND INNOVATION-PROCEEDINGS OF MIE 2023
(2023)
Proceedings Paper
Computer Science, Interdisciplinary Applications
Christopher Hawthorne, Luis Marco Ruiz, Guillermo Lopez Campos
Summary: This study used text mining techniques to map ontology terms related to human phenotypes and exposome factors, facilitating the integration of exposomic and clinical data.
CARING IS SHARING-EXPLOITING THE VALUE IN DATA FOR HEALTH AND INNOVATION-PROCEEDINGS OF MIE 2023
(2023)
Article
Veterinary Sciences
Jose Antonio Orden, Abel Martinez-Rodrigo, Ana Isabel Vela, Jose Francisco Fernandez-Garayzabal, Clara Hurtado-Morillas, Alicia Mas, Gustavo Dominguez-Bernal
Summary: The presence of non-E. coli Enterobacteriaceae in raccoon feces in the Madrid region of Spain has been discovered, highlighting the potential risk they pose to humans and livestock due to their pathogenic nature and antimicrobial resistance.
JOURNAL OF VETERINARY RESEARCH
(2022)
