Article
Biochemistry & Molecular Biology
Machi Yamamoto, Mana Miyoshi, Kamino Morioka, Takakazu Mitani, Tomohide Takaya
Summary: A study found that the myogenetic oligodeoxynucleotide iSN04 can inhibit inflammatory responses in muscle cells by suppressing the activity of the nuclear transcription factor NF-kB, thereby promoting myocyte differentiation. The study also observed anti-inflammatory effects of iSN04 in rat smooth muscle cells and murine adipocyte-like fibroblast cells, suggesting its potential use in preventing inflammation caused by metabolic disorders.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Rukmini Mukherjee, Anshu Bhattacharya, Denisa Bojkova, Ahmad Reza Mehdipour, Donghyuk Shin, Khadija Shahed Khan, Hayley Hei-Yin Cheung, Kam-Bo Wong, Wai-Lung Ng, Jindrich Cinatl, Paul P. Geurink, Gerbrand J. van der Heden van Noort, Krishnaraj Rajalingam, Sandra Ciesek, Gerhard Hummer, Ivan Dikic
Summary: Famotidine has no effect on viral replication but can modulate specific signaling pathways, reducing TLR3-dependent signaling processes and subsequent activation of IRF3 and the NF-kappa B pathway, leading to control of antiviral and inflammatory responses.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Hiroyasu Sakai, Yuta Suzuki, Yu Miyauchi, Fumiaki Sato, Yusuke Ando, Risako Kon, Nobutomo Ikarashi, Yoshihiko Chiba, Junzo Kamei, Tomoo Hosoe
Summary: Cancer patients often experience muscle atrophy, which is worsened by the decreased muscle regenerative capacity. Cisplatin, a cancer chemotherapeutic agent, has been found to cause muscle atrophy. This study explored the effects of cisplatin on the differentiation of myoblasts into myotubes. The results showed that cisplatin suppresses myogenesis and inhibits the expression of Sparcl1, which promotes C2C12 cell differentiation during myogenesis.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cheol Park, Hee-Jae Cha, Hyun Hwangbo, Seon Yeong Ji, Da Hye Kim, Min Yeong Kim, EunJin Bang, Su Hyun Hong, Sung Ok Kim, Soon-Jeong Jeong, Hyesook Lee, Sung-Kwon Moon, Jung-Hyun Shim, Gi-Young Kim, Suengmok Cho, Yung Hyun Choi
Summary: Phloroglucinol, a polyphenolic compound, has potent antioxidant activity in mouse myoblasts and may have therapeutic benefits for oxidative-stress-mediated muscle disease by activating Nrf2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Cheol Park, Hyesook Lee, Suhyun Hong, Ilandarage Menu Neelaka Molagoda, Jin-Woo Jeong, Cheng-Yun Jin, Gi-Young Kim, Sung Hyun Choi, Sang Hoon Hong, Yung Hyun Choi
Summary: The study demonstrated the inhibitory effect of trans-cinnamaldehyde on inflammatory and oxidative stress induced by lipopolysaccharide in muscle cells by targeting the TLR4/NF-κ B pathway, potentially mediated by NOXs and Nrf2/HO-1 pathways.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yujuan Chen, Junhong Lin, Yao Zhao, Xianping Ma, Huashan Yi
Summary: TLR3 is unique among TLR family members in its dependence on TRIF for RNA sensing. However, details of how the TLR3-TRIF signaling pathway functions in antiviral response remain unclear. Better understanding of TLR3 could aid in disease diagnosis and novel treatments for viral diseases.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2021)
Article
Biochemistry & Molecular Biology
Israr Ahmad, Tahseen H. Nasti, Heba M. Rihan, Hugo Jimenez, Craig A. Elmets, Nabiha Yusuf
Summary: Experimental findings suggest a crucial role of TLR4 in UVB-induced skin cancer development, with TLR4-deficient mice showing lower tumor incidence and shorter tumor latency compared to TLR4-proficient mice. The inflammatory response and immune cell levels were higher in TLR4-proficient mice than in TLR4-deficient mice.
MOLECULAR CARCINOGENESIS
(2021)
Article
Biochemistry & Molecular Biology
Tsukasa Tominari, Miyuki Akita, Chiho Matsumoto, Michiko Hirata, Shosei Yoshinouchi, Yuki Tanaka, Kento Karouji, Yoshifumi Itoh, Takayuki Maruyama, Chisato Miyaura, Yukihiro Numabe, Masaki Inada
Summary: TLR3 signaling plays a crucial role in periodontal bone resorption by controlling the production of PGE2 and inducing the differentiation and survival of mature osteoclasts.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jiyeon Choi, Joo Weon Lim, Hyeyoung Kim
Summary: House dust mites are crucial in airway inflammation, activating Toll-like receptor 4 and inducing inflammatory cytokine expression in respiratory epithelial cells. The antioxidant lycopene can inhibit these effects by suppressing TLR4 activation and reducing ROS levels in cells.
Review
Biochemistry & Molecular Biology
Natticha Sumneang, Pongpan Tanajak, Thura Tun Oo
Summary: Doxorubicin (Dox) is commonly used chemotherapeutic drug, but its cardiotoxicity reduces its therapeutic efficacy. Dox-induced cardiac inflammation is considered as one of the underlying mechanisms involved in Dox-induced cardiotoxicity. Understanding the role of the Toll-like receptor 4 (TLR4) signaling pathway in Dox-induced cardiac inflammation might be beneficial for developing potential therapeutic strategies.
Article
Immunology
Cristina Cappelletti, Raffaella Brugnoni, Silvia Bonanno, Francesca Andreetta, Franco Salerno, Eleonora Canioni, Gaetano Nicola Alfio Vattemi, Paola Tonin, Renato Mantegazza, Lorenzo Maggi
Summary: This study aimed to investigate whether the immune system could serve as a source of potential biomarkers for stratifying immune-mediated necrotizing myopathies (IMNMs) subtypes. The results showed a significant preponderance of M1 macrophages in muscle samples of both SRP+ and HMGCR(+) IMNM patients. Furthermore, TLR4 and IL-7 were identified as potential immune biomarkers for differentiating between SRP+ and HMGCR+ patients.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Endocrinology & Metabolism
Hai-Yun Guan, He-Xia Xia, Xiu-Ying Chen, Lu Wang, Zhi-Jing Tang, Wei Zhang
Summary: The activation of TLR4 was found to suppress CYP19A1 expression and E2 secretion in human granulosa cells via NF-κB signaling, which has implications for follicular development and estrogen synthesis regulation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Virology
Melanie Werner, Stefan Schefczyk, Martin Trippler, Juergen W. Treckmann, Hideo A. Baba, Guido Gerken, Joerg F. Schlaak, Ruth Broering
Summary: Non-parenchymal liver cells play a crucial role in defense against hepatotropic viruses by mediating inflammatory responses and antiviral activities via secretion of cytokines and interferons. Liver sinusoidal endothelial cells exhibit heightened responsiveness to viral stimuli in chronic hepatitis C virus infection.
Article
Cell Biology
Evangelos Zevolis, Anastassios Philippou, Athanasios Moustogiannis, Antonios Chatzigeorgiou, Michael Koutsilieris
Summary: This study investigated the cellular and molecular events in cardiomyocyte-like H9C2 cells during differentiation and the responses of the differentiated cells to mechanical stretching. The results showed that altering the strain and frequency of mechanical loading applied on differentiated H9C2 cardiomyoblasts can regulate their anabolic/survival program, with low-strain/frequency stretching being most effective in inducing beneficial responses.
Article
Geriatrics & Gerontology
Mari Kamiya, Fumitaka Mizoguchi, Shinsuke Yasuda
Summary: GLP-1R agonist could be a novel therapy for PM that recovers muscle weakness and suppresses muscle inflammation through inhibiting muscle fibre necroptosis.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Biochemical Research Methods
Amelie Barczyk, Helene Bauderlique-Le Roy, Nathalie Jouy, Nicolas Renault, Audrey Hottin, Regis Millet, Valerie Vouret-Craviari, Sahil Adriouch, Thierry Idziorek, Xavier Dezitter
Summary: P2RX7 is a poorly selective ATP-gated ion channel with important physiological and pathological implications, making it a potential therapeutic target in several diseases. Novel screening methods based on flow cytometry and spectral flow cytometry have been developed to efficiently measure P2RX7 activity and assess the efficacy of modulators. Spectral cytometry appears to be a useful tool for drug candidate screening by simultaneously evaluating multiple biological activities and correcting for the autofluorescence of compounds screened.
Article
Multidisciplinary Sciences
Laetitia Douguet, Serena Janho Dit Hreich, Jonathan Benzaquen, Laetitia Seguin, Thierry Juhel, Xavier Dezitter, Christophe Duranton, Bernhard Ryffel, Jean Kanellopoulos, Cecile Delarasse, Nicolas Renault, Christophe Furman, Germain Homerin, Chloe Feral, Julien Cherfils-Vicini, Regis Millet, Sahil Adriouch, Alina Ghinet, Paul Hofman, Valerie Vouret-Craviari
Summary: A small subpopulation of non-small cell lung cancer patients respond to immunotherapies, and the development of HEI3090 as a chemical modulator of the purinergic P2RX7 receptor may enhance anti-tumor immune responses and sensitize lung cancer to PD-1 therapy in preclinical models.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Medicine, Research & Experimental
Sahil Adriouch, Pablo Pelegrin
Summary: The study developed specific anti-ASC nanobodies that effectively disaggregate formed ASC oligomers and treat inflammatory diseases in animal models. This approach represents a novel biologic-based treatment for inflammasomes-initiated inflammatory diseases.
EMBO MOLECULAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Gabriel Ichim, Benjamin Gibert, Sahil Adriouch, Catherine Brenner, Nathalie Davoust, Solange Desagher, David Devos, Svetlana Dokudovskaya, Laurence Dubrez, Jerome Estaquier, Germain Gillet, Isabelle Guenal, Philippe P. Juin, Guido Kroemer, Patrick Legembre, Romain Levayer, Stephen Manon, Patrick Mehlen, Olivier Meurette, Olivier Micheau, Bernar Mignotte, Florence Nguyen-Khac, Nikolay Popgeorgiev, Jean-Lu Poyet, Muriel Priault, Jean-Ehrlan Ricci, Franck B. Riquet, Santos A. Susin, Magal Suzanne, Pierre Vacher, Ludivine Walter, Bertran Mollereau
Summary: Since the Nobel Prize was awarded more than twenty years ago for discovering the core apoptotic pathway in C. elegans, researchers around the world have conducted extensive research on apoptosis and various other forms of regulated cell death. Although there are still many aspects of regulated cell death that need to be clarified in specific cell subtypes and disease conditions, the last decade has seen the description of multiple cell death modalities, some of which have been successfully used in clinical therapy. To keep research into cell death alive, francophone researchers from several institutions in France and Belgium established the French Cell Death Research Network (FCDRN), which is at the forefront of emerging topics in cell death research. These research efforts will enhance our mechanistic knowledge of regulated cell death and its therapeutic applications in the coming years.
Article
Pharmacology & Pharmacy
Carolina Pinto-Espinoza, Charlotte Guillou, Bjoern Rissiek, Maximilian Wilmes, Ehsan Javidi, Nicole Schwarz, Marten Junge, Friedrich Haag, Nastassia Liaukouskaya, Nicola Wanner, Annette Nicke, Catelijne Stortelers, Yossan-Var Tan, Sahil Adriouch, Tim Magnus, Friedrich Koch-Nolte
Summary: This study evaluated the ability of P2X7-specific nanobodies to reach and block P2X7 on microglia. The results showed that intracerebral injection of nanobodies achieved faster and more effective blockade of microglial P2X7 compared to intravenous injection. Additionally, the endogenous production of nanobody-encoding AAVs resulted in long-term occupancy and blockade of P2X7 on microglia.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Melanie Demeules, Allan Scarpitta, Romain Hardet, Henri Gonde, Catalina Abad, Marine Blandin, Stephan Menzel, Yinghui Duan, Bjoern Rissiek, Tim Magnus, Anna Marei Mann, Friedrich Koch-Nolte, Sahil Adriouch
Summary: Adenosine triphosphate (ATP) is a danger signal that accumulates in injured tissues, inflammatory sites, and the tumor microenvironment. It promotes tumor growth and anti-tumor immune responses via the P2X7 receptor. P2X7, CD39, and CD73 are novel purinergic checkpoints that can be targeted for cancer treatment. Blocking P2X7 inhibits tumor growth, while a nanobody-based biologic targeting P2X7 enhances tumor control and immune responses when used in combination with chemotherapy. A bi-specific nanobody-based biologic targeting PD-L1 and CD73 has a potent anti-tumor effect.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Virgilia Sahiri, Jonathan Caron, Elena Roger, Christophe Desterke, Khalil Ghachem, Inna Mohamadou, Justine Serre, Niki Prakoura, Soraya Fellahi, Sandrine Placier, Sahil Adriouch, Lu Zhang, Christos E. Chadjichristos, Christos Chatziantoniou, Hans Kristian Lorenzo, Jean-Jacques Boffa
Summary: A study found that the progression of FSGS is correlated with podocyte apoptosis, in which ISM1 plays an important role. Overexpression of ISM1 was observed during FSGS progression and localized in glomeruli, while it partially restored when the disease improved. Further in vitro experiments demonstrated that ISM1 interacts with its receptors GRP78 and integrin alpha v beta 5 on podocytes, inducing apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ronald Sluyter, Sahil Adriouch, Stephen J. Fuller, Annette Nicke, Reece A. Sophocleous, Debbie Watson
Summary: The P2X7 receptor is a ligand-gated cation channel that is activated by extracellular adenosine 5'-triphosphate. Animal models have played a crucial role in understanding the physiological and pathophysiological functions of P2X7 in human health and disease. This article highlights the contributions of animal models in generating tools for studying P2X7, such as specific antibodies, recombinant receptors, and pharmacokinetic testing of P2X7 antagonists. It also discusses the use of mouse, rat, and other animal models in studying P2X7, including disease models, knockout mice, reporter mice, and humanized mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Ann-Christin Franke, Romain Hardet, Lisa Prager, Martin Bentler, Melanie Demeules, Philipp John-Neek, Nico Martin Jaeschke, Teng Cheong Ha, Ulrich Thorsten Hacker, Sahil Adriouch, Hildegard Buening
Summary: Immunotherapy combined with anti-tumor vaccines has shown promising results in improving treatment outcomes for various cancer types. In this study, adeno-associated virus (AAV) vectors were used as a potent cancer vaccine platform that induces strong and long-lasting antigen-specific immune responses. The AAV vector-based vaccine effectively protected mice against tumor growth in different tumor models, even when challenged with tumors at a late stage. This approach of decorating AAV vector particles with antigens by capsid engineering represents a versatile and efficient vaccine strategy for cancer immunotherapy.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Review
Biotechnology & Applied Microbiology
Helena Costa-Verdera, Carmen Unzu, Erika Valeri, Sahil Adriouch, Gloria Gonzalez Aseguinolaza, Federico Mingozzi, Anna Kajaste-Rudnitski
Summary: As the clinical experience in AAV vector-based gene therapies grows, there is a need to better understand and control the host immune responses. Our current understanding of the immune responses to AAV gene therapies is limited, which is further hindered by the lack of replication of clinical findings in preclinical animal models.
HUMAN GENE THERAPY
(2023)
Article
Medicine, Research & Experimental
Martin Bentler, Romain Hardet, Moritz Ertelt, Daniela Rudolf, Dorota Kaniowska, Andreas Schneider, Florian W. R. Vondran, Clara T. Schoeder, Marion Delphin, Julie Lucifora, Michael Ott, Ulrich T. Hacker, Sahil Adriouch, Hildegard Buening
Summary: In this study, the researchers successfully reduced immune responses in gene therapy by inserting a peptide into the AAV2 capsid. The novel capsid variant, AAV2.MB453, showed improved transduction efficiency and reduced innate and adaptive immune responses in human cells.
MOLECULAR THERAPY METHODS & CLINICAL DEVELOPMENT
(2023)
Meeting Abstract
Biotechnology & Applied Microbiology
A. Scarpitta, R. Hardet, M. Bentler, M. Demeules, M. Blandin, U. Hacker, H. Buening, O. Boyer, S. Adriouch
HUMAN GENE THERAPY
(2022)
Meeting Abstract
Biotechnology & Applied Microbiology
W. Schaefer, N. Richter, S. Jargalsaikhan, F. Haag, I. Braren, C. Thirion, S. Adriouch, F. Koch-Nolte, A. M. Mann
HUMAN GENE THERAPY
(2022)
Meeting Abstract
Clinical Neurology
C. Guillou, M. Fourny, C. Abad, S. Adriouch, Y. -V. Tan
MULTIPLE SCLEROSIS JOURNAL
(2022)
Meeting Abstract
Immunology
Henri Gonde, Melanie Demeules, Romain Hardet, Allan Scarpitta, Marten Junge, Carolina Pinto Espinoza, Remi Varin, Friedrich Koch Nolte, Olivier Boyer, Sahil Adriouch
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)