Fitness Loss and Library Size Determination in Saturation Mutagenesis

The Importance of Additive and Non-Additive Mutational Effects in Protein Engineering

(2013) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Principles for designing ideal protein structures

(2012) Nobuyasu Koga et al.   NATURE

Reducing Codon Redundancy and Screening Effort of Combinatorial Protein Libraries Created by Saturation Mutagenesis

(2012) Sabrina Kille et al.   ACS Synthetic Biology

When Second Best Is Good Enough: Another Probabilistic Look at Saturation Mutagenesis

(2011) Yuval Nov   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Revisiting the Lipase from Pseudomonas aeruginosa: Directed Evolution of Substrate Acceptance and Enantioselectivity Using Iterative Saturation Mutagenesis

(2011) Shreenath Prasad et al.   CHEMPHYSCHEM

Directed Evolution of Sulfotransferases and Paraoxonases by Ancestral Libraries

(2011) Uria Alcolombri et al.   JOURNAL OF MOLECULAR BIOLOGY

Improving Biocatalyst Performance by Integrating Statistical Methods into Protein Engineering

(2010) M. Brouk et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A pH-Based High-Throughput Screening of Sucrose-Utilizing Transglucosidases for the Development of Enzymatic Glucosylation Tools

(2010) Elise Champion et al.   ChemCatChem

Beyond directed evolution—semi-rational protein engineering and design

(2010) Stefan Lutz   CURRENT OPINION IN BIOTECHNOLOGY

Engineering Human IgG1 Affinity to Human Neonatal Fc Receptor: Impact of Affinity Improvement on Pharmacokinetics in Primates

(2009) Y. A. Yeung et al.   JOURNAL OF IMMUNOLOGY

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

HotSpot Wizard: a web server for identification of hot spots in protein engineering

(2009) A. Pavelka et al.   NUCLEIC ACIDS RESEARCH

Adaptive landscapes and protein evolution

(2009) M. Carneiro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein Engineering of Toluene Monooxygenases for Synthesis of Chiral Sulfoxides

(2008) R. Feingersch et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Protein engineering of hydrogenase 3 to enhance hydrogen production

(2008) Toshinari Maeda et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

(2008) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Optimizing Glycosyltransferase Specificity via “Hot Spot” Saturation Mutagenesis Presents a Catalyst for Novobiocin Glycorandomization

(2008) Gavin J. Williams et al.   CHEMISTRY & BIOLOGY

Loop Grafting of Bacillus subtilis Lipase A: Inversion of Enantioselectivity

(2008) Ykelien L. Boersma et al.   CHEMISTRY & BIOLOGY

Improving the photostability of bright monomeric orange and red fluorescent proteins

(2008) Nathan C Shaner et al.   NATURE METHODS

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

(2008) A. E. Firth et al.   NUCLEIC ACIDS RESEARCH