Article
Biochemistry & Molecular Biology
Jinling Zhang, Yong Wang
Summary: The study showed that TGF-beta 2 might alter the expression of miRNAs in EVs derived from HTMCs, potentially contributing to the pathogenesis of POAG. These findings could provide valuable information for the diagnosis and treatment of POAG.
DNA AND CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hsin-Yi Chen, Hsiu-Chuan Chou, Yi-Jung Ho, Shing-Jyh Chang, En-Chi Liao, Yu-Shan Wei, Meng-Wei Lin, Yi-Shiuan Wang, Yu-An Chien, Xin-Ru Yu, Hsiang-Yu Kung, Chu-Chun Yang, Jia-Yu Chen, Hong-Lin Chan, Mei-Lan Ko
Summary: The study demonstrates the significant role of TGF-beta in oxidative stress-induced damage, showing its ability to protect HTMCs from injury by regulating signaling transduction pathways.
Article
Biochemistry & Molecular Biology
Megumi Watanabe, Tatsuya Sato, Yuri Tsugeno, Araya Umetsu, Soma Suzuki, Masato Furuhashi, Yosuke Ida, Fumihito Hikage, Hiroshi Ohguro
Summary: Real-time cellular metabolism analysis indicated that TGF-beta 2 significantly induced a shift in cellular energy metabolism, while DEX had a similar but lesser effect. Direct compression stresses on 3D HTM spheroids resulted in fluctuation of gene expressions of ECM proteins, inflammatory cytokines, and ER stress-related factors. However, compression stresses on TGF-beta 2- or DEX-treated 3D HTM spheroids induced downregulation of inflammatory cytokines and ER stress-related factors.
Article
Medicine, General & Internal
Soma Suzuki, Masato Furuhashi, Yuri Tsugeno, Araya Umetsu, Yosuke Ida, Fumihito Hikage, Hiroshi Ohguro, Megumi Watanabe
Summary: This study compares the drug-induced efficacies between omidenepag (OMD), an EP2 agonist, and prostaglandin F2 alpha (PGF2 alpha) on glaucomatous trabecular meshwork (TM) cells. The findings suggest that PGF2 alpha and OMD differently modulate the permeability of the cells and the physical properties.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Li Li, Qian Liu, Lijuan Shi, Xibin Zhou, Wei Wu, Xue Wang, Lihua Wang, Zhihong Wu
Summary: This study investigated the anti-fibrotic effects of baicalin on human trabecular meshwork (HTM) cells and the underlying regulatory mechanisms. The findings suggest that baicalin can prevent TGF-beta 2-induced extracellular matrix deposition and alpha-SMA expression in HTM cells by inhibiting the MyD88/NF-kappa B pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Ophthalmology
Vidhya R. Rao, Evan B. Stubbs
Summary: TGF-beta 2 promotes oxidative stress in primary human TM cells by selectively increasing expression of NADPH oxidase 4. Dysregulation of redox equilibrium by induction of NADPH oxidase 4 expression appears to be a key early event involved in the pathologic profibrotic responses elicited by TGF-beta 2 canonical signaling, including ECM remodeling, filamentous actin stress fiber formation, and alpha SMA expression.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Cell Biology
Mark S. S. Filla, Jennifer A. A. Faralli, Caleigh R. R. Dunn, Haania Khan, Donna M. M. Peters
Summary: NFATc1 controls the transcription of TGF beta 2 in human TM cells. Dex significantly increases TGF beta 2 mRNA expression in proliferating cells, but not in quiescent or serum-starved cells.
Article
Cell Biology
Mark S. Filla, Kristy K. Meyer, Jennifer A. Faralli, Donna M. Peters
Summary: Studies indicate that activation of αvβ3 integrin signaling may significantly impact TM cell function and POAG pathogenesis; overexpression of CA-αvβ3 integrin leads to significant differential gene expression involving multiple signaling pathways.
Article
Cell Biology
Xuejing Yan, Shen Wu, Qian Liu, Ying Cheng, Jingxue Zhang, Ningli Wang
Summary: The study demonstrates that the activation of autophagy is correlated with the pathogenesis of primary open angle glaucoma (POAG) associated with elevated intraocular pressure (IOP). The N450Y mutation of myocilin induces autophagy, which worsens cell viability. A transgenic mouse model with the N450Y myocilin mutation exhibits glaucoma phenotypes and altered aqueous humor dynamics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Myoung Sup Shim, April Nettesheim, Angela Dixon, Paloma B. Liton
Summary: This study identified primary cilia as the mechanosensor for cyclic mechanical stretch-induced autophagy in trabecular meshwork cells. The cross-regulation between AKT1 and noncanonical SMAD2/3 signaling was found to be critical in primary cilia-mediated activation of autophagy by mechanical stretch. Removal of primary cilia disrupted the homeostatic intraocular pressure compensatory response, highlighting the importance of primary cilia-mediated autophagy in regulating intraocular pressure homeostasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Yogapriya Sundaresan, Krishnadas Subbiah Ramasamy, Muthukkaruppan Veerappan, Gowri Priya Chidambaranathan
Summary: The study identified adult stem cells in the human trabecular meshwork (TM) responsible for tissue homeostasis maintenance, based on high expression of ABCG2 and p75 markers. The cultured TM cells showed label retaining property and ability to form spheres, with differentiation potential into TM cells and phagocytic abilities similar to primary TM cells.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Ophthalmology
Rajiv Rangan, Rafael Sad do Valle, Tara Tovar-Vidales
Summary: This study investigated the expression of procollagen C-proteinase enhancer 1 (PCOLCE1) in human trabecular meshwork (TM) and its potential alteration in glaucomatous conditions. The findings suggest that PCOLCE1 is expressed in TM tissues and cells and its expression may be downregulated by TGF82 treatment.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Genetics & Heredity
John Knox, George Bou-Gharios, Kevin J. Hamill, Colin E. Willoughby
Summary: Inhibiting the upregulation of connective tissue growth factor (CTGF) can reduce the contractility of trabecular meshwork (TM) cells, providing a potential treatment for primary open-angle glaucoma (POAG).
Article
Materials Science, Biomaterials
Bikram Adhikari, Matthew J. Osmond, Mina B. Pantcheva, Melissa D. Krebs
Summary: This study examines the effects of exogenous glycosaminoglycans and dexamethasone on human trabecular meshwork cells, revealing their influence on the expression levels of ECM genes and proteins, providing insights into the pathogenesis of glaucoma.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Ophthalmology
Avani A. Mody, J. Cameron Millar, Abbot F. Clark
Summary: Elevated intraocular pressure (IOP) in primary open-angle glaucoma (POAG) is a major risk factor for irreversible vision loss. Over-expression of ID1 and ID3 can suppress TGF beta 2-induced ocular hypertension and decreased aqueous humor (AH) outflow facility. These proteins may be potential candidates for future IOP-lowering treatments in POAG.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Mark Basche, Daniel Kampik, Satoshi Kawasaki, Matthew J. Branch, Martha Robinson, D. Frank Larkin, Alexander J. Smith, Robin R. Ali
HUMAN GENE THERAPY
(2018)
Article
Ophthalmology
I. S. Tarau, X. Wang, M. Nentwich, J. Hillenkamp, D. Kampik
Article
Clinical Neurology
Dimitar Evdokimov, Johanna Frank, Alexander Klitsch, Stefan Unterecker, Bodo Warrings, Jordi Serra, Aikaterini Papagianni, Nadine Saffer, Caren Meyer zu Altenschildesche, Daniel Kampik, Rayaz A. Malik, Claudia Sommer, Nurcan Uceyler
ANNALS OF NEUROLOGY
(2019)
Article
Clinical Neurology
Alexander Klitsch, Dimitar Evdokimov, Johanna Frank, Dominique Thomas, Nadine Saffer, Caren Meyer zu Altenschildesche, Marco Sisignano, Daniel Kampik, Rayaz A. Malik, Claudia Sommer, Nurcan Ueceyler
JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM
(2020)
Article
Ophthalmology
Celine Trigaux, Sabine Salla, Jan Schroeter, Theofilos Tourtas, Henning Thomasen, Philip Maier, Olaf J. C. Hellwinkel, Ilka Wittmershaus, Patrick R. Merz, Berthold Seitz, Bernhard Noelle, Norbert Schrage, Sigrid Roters, Melissa Apel, Andrea Gareiss-Lok, Constantin E. Uhlig, Sebastian Thaler, Florian Raber, Daniel Kampik, Gerd Geerling, Johannes Menzel-Severing
Summary: During the COVID-19 pandemic, German eye banks have implemented measures such as reducing donor recruitment and additional diagnostic screening to address potential risks associated with corneal donation.
CURRENT EYE RESEARCH
(2021)
Article
Ophthalmology
Pia Stockinger, Andreas Berlin, Daniel Kampik, Christine Schmitt, Jost Hillenkamp, Jeffrey D. Messinger, Martina C. Herwig-Carl, Thomas Ach
Article
Ophthalmology
Sibylle Katharina Scholtz, Gerd Uwe Auffarth, Olaf Hellwinkel, Daniel Kampik, Philip Christian Maier, Berthold Seitz, Thomas Wegner, Frank Krogmann, Katja Rosenbaum, Gerd Geerling
Summary: Since the first successful keratoplasty in 1905, there has been a need to store corneas for transplantation. R. Townley Paton founded the first eye bank in New York in 1944. LIONS Clubs International has been involved in establishing eye banks worldwide, providing funding and networking support. Eye banks play a crucial role in meeting the demand for donor corneas, but continuous improvement in techniques and equipment is necessary.
KLINISCHE MONATSBLATTER FUR AUGENHEILKUNDE
(2021)
Article
Ophthalmology
Pia Stockinger, Andreas Berlin, Daniel Kampik, Christine Schmitt, Jost Hillenkamp, Jeffrey D. Messinger, Martina C. Herwig-Carl, Thomas Ach
Summary: This study conducted a direct comparison between high-resolution in vivo retinal imaging and ex vivo histological preparations to enhance understanding of retinal diseases. However, the method is currently limited due to restricted availability of human donor tissue.
Article
Microbiology
Grit Walther, Anna Zimmermann, Johanna Theuersbacher, Kerstin Kaerger, Marie Von Lilienfeld-Toal, Mathias Roth, Daniel Kampik, Gerd Geerling, Oliver Kurzai
Summary: "Fungal eye infections can lead to vision loss and blindness. This study in Germany identified Fusarium as the most common genus causing eye infections, with some species showing resistance to traditional antifungal treatments. Proper species identification is crucial for effective treatment."
Article
Medicine, Research & Experimental
Alexander M. Aldejohann, Johanna Theuersbacher, Lukas Haug, Olga S. Lamm, Grit Walther, Oliver Kurzai, Jost Hillenkamp, Daniel Kampik
Summary: A case of Kluyveromyces marxianus keratitis was reported in a patient with Fuchs endothelial disease nine months after Descemet's membrane endothelial keratoplasty. Management of keratomycosis in patients with endothelial grafts remains challenging despite immediate antifungal treatment. Penetrating keratoplasty was eventually required in this particular case.
MEDICAL MYCOLOGY CASE REPORTS
(2021)
Article
Clinical Neurology
Nadine Egenolf, Caren Meyer zu Altenschildesche, Luisa Kress, Katja Eggermann, Barbara Namer, Franziska Gross, Alexander Klitsch, Tobias Malzacher, Daniel Kampik, Rayaz A. Malik, Ingo Kurth, Claudia Sommer, Nurcan Uceyler
Summary: This case-control study suggests that the diagnosis of small fiber neuropathy (SFN) in patients with pain of uncertain origin can be primarily based on the results of neurological examination and distal intraepidermal nerve fiber density (IENFD), with further detailed phenotyping recommended in specialized centers. Abnormal distal IENFD and neurological examination were the most common indicators of small fiber disease, with additional tests like corneal confocal microscopy (CCM) and pain-related evoked potentials (PREP) increasing the number of patients with small fiber impairment. Genetic testing revealed potentially pathogenic gene variants in a subset of the index patients.
THERAPEUTIC ADVANCES IN NEUROLOGICAL DISORDERS
(2021)
Meeting Abstract
Ophthalmology
Franziska Seifert, Franziska Seufert, Dorothee Hommes, Gunda Kann, Luisa Thederan, Jost Hillenkamp, Daniel Kampik
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Meeting Abstract
Ophthalmology
Daniel Kampik, Ioana-Sandra Tarau, Hong Han, Heike Walles, Florian Kai Groeber-Becker, Jost Hillenkamp, Christian Lotz
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Article
Ophthalmology
Daniel Kampik, Mark Basche, Anastasios Georgiadis, Ulrich F. O. Luhmann, D. Frank Larkin, Alexander J. Smith, Robin R. Ali
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2019)
Meeting Abstract
Clinical Neurology
N. Saffer, L. Kress, K. Eggermann, B. Namer, T. Malzacher, A. Klitsch, D. Kampik, R. Malik, I. Kurth, C. Sommer, N. Ueceyler
EUROPEAN JOURNAL OF NEUROLOGY
(2019)