Article
Oncology
Susanne Grube, Diana Freitag, Rolf Kalff, Christian Ewald, Jan Walter
Summary: Characterization of primary adherent glioblastoma cell lines is essential to reliably identify these cell lines. These cell lines exhibit high growth rate, diverse morphologies, and capabilities of migration and colony formation. Immunocytochemical analysis is crucial for validating the expression of specific markers in these cell lines.
Article
Obstetrics & Gynecology
Kelly McCarter, Robert Setton, Alice Chung, Anjile Ana, Zev Rosenwaks, Steven Spandorfer
Summary: The study compared pregnancy outcomes in couples with non-male factor infertility using IVF and intracytoplasmic sperm injection cycles with fresh and frozen ejaculated spermatozoa. Results showed that frozen sperm resulted in lower delivery rates compared to fresh sperm, with significantly higher pregnancy loss rates. Adjusted odds for delivery were lower in the frozen group, while odds for pregnancy and implantation were not significantly different between fresh and frozen sperm groups.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Surgery
Sharven Taghavi, Olan Jackson-Weaver, Sarah Abdullah, Amy Goldberg, Shaun Lawicki, Mary Killackey, Juan Duchesne, Derek Pociask, Chad Steele, Jay Kolls
Summary: The study found that fresh frozen plasma (FFP) had lower levels of growth factors and pro-coagulants compared to never-frozen plasma (NFP), while levels of pro-inflammatory chemokines were higher. Further research is needed to determine if these cytokines impact outcomes in trauma patients receiving transfusions.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Inna E. Pristyazhnyuk, Lyubov P. Malinovskaya, Pavel M. Borodin
Summary: We developed a simple method for establishing primary somatic cell cultures from the ovaries of great tits and the testes of ten Passerine species. The ovary-derived cell cultures showed high proliferative activity, while the testis-derived cell cultures had lower proliferation potential. However, both cell cultures provided sufficient material for genetic modification and cytogenetic studies.
Article
Oncology
Julia A. Schulz, Louis T. Rodgers, Richard J. Kryscio, Anika M. S. Hartz, Bjorn Bauer
Summary: This study characterized two human GBM models (U87-luc2, U251-RedFLuc) and found that they recapitulate different aspects of GBM disease, with U251-RedFLuc cells showing higher invasiveness. The findings highlight the importance of considering GBM heterogeneity in preclinical research.
Article
Cell Biology
Yuan Gao, Ji Dong, Shuyue Qi, Xin Zhou, Xinglong Wu, Wendong Wang, Lu Wen, Wei Fu, Fuchou Tang
Summary: By establishing a culture system for adult human gastric epithelial progenitor-like cells, this study investigates the self-renewal and differentiation potentials of human gastric epithelial stem/progenitor cells and their relevance to gastric diseases.
CELL PROLIFERATION
(2023)
Article
Oncology
Fangling Cheng, Xueyan Wan, Baofeng Wang, Youwei Li, Peng Peng, Sanpeng Xu, Chao Han, Feng Mao, Dongsheng Guo
Summary: This study successfully established a novel primary glioblastoma cell line, GWH04, and examined its genetic characteristics and tumor parameters. The results indicated that GWH04 had similar proliferation rate as U87 cell line, but faster than GL15 cell line, and possessed high capacity for clone formation and tumor formation. GWH04 showed resistance to temozolomide in drug sensitivity test. The genetic analysis revealed aneuploid karyotype and chromosomal structural abnormalities in GWH04, as well as mutations in several key genes. These findings suggest that GWH04 will be a valuable tool for human GBM studies in both in vitro and in vivo settings.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chang Sik Cho, Dong Hyun Jo, Jin Hyoung Kim, Jeong Hun Kim
Summary: This study established carboplatin-resistant retinoblastoma cell lines, providing a tool for research on the treatment of chemoresistant retinoblastoma.
MOLECULES AND CELLS
(2022)
Article
Cell Biology
Haihuan Lin, Juliane Mensch, Maria Haschke, Kathrin Jager, Brigitte Kottgen, Jens Dernedde, Evelyn Orso, Michael Walter
Summary: Establishing five immortalized HGP fibroblast cell lines using retroviral infection with the catalytic subunit of hTERT demonstrated enhanced proliferative lifespan and reduced senescence signs, with growth increase and phenotype improvement independent of telomere elongation. The initial telomeric stabilization after hTERT infection and relatively low amounts of hTERT mRNA were sufficient for phenotype improvement, suggesting implications for therapeutic strategies in HGP and other premature aging syndromes.
Article
Biochemistry & Molecular Biology
Chang Sik Cho, Dong Hyun Jo, Jin Hyoung Kim, Jeong Hun Kim
Summary: The study established carboplatin-resistant retinoblastoma cell lines, which can be used to investigate chemoresistance in retinoblastoma and develop potential therapeutics.
MOLECULES AND CELLS
(2022)
Article
Agriculture, Dairy & Animal Science
Jiali Li, Bohao Zhao, Xiyu Zhang, Yingying Dai, Naisu Yang, Zhiyuan Bao, Yang Chen, Yan Liu, Xinsheng Wu
Summary: In this study, immortalized DPC lines were established by introducing SV40T antigen into early passage rabbit vibrissae DPCs. Among these cell lines, R-7 showed the best survival rate and preserved the basic characteristics of primary DPCs. These immortalized DPCs have potential application for future hair research.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Stine Bottcher Jacobsen, Jacob Tfelt-Hansen, Morten Holdgaard Smerup, Jeppe Dyrberg Andersen, Niels Morling
Summary: The use of fresh tissue for molecular studies is preferred but often impossible. Frozen or formalin-fixed, paraffin-embedded (FFPE) tissues are widely used and valuable for retrospective studies. RNA extracted from FFPE tissues is highly degraded and yields different gene expression profiles compared to fresh and frozen tissues. However, protein-coding transcripts show high correlations among different storage conditions. Proper quality control and caution regarding technical variability are required when using frozen and FFPE tissues for gene expression analyses.
Article
Biochemistry & Molecular Biology
Fernanda F. da Silva, Fernanda C. S. Lupinacci, Bruno D. S. Elias, Adriano O. Beserra, Paulo Sanematsu, Martin Roffe, Leslie D. Kulikowski, Felipe D'almeida Costa, Tiago G. Santos, Glaucia N. M. Hajj
Summary: This study successfully established and characterized the GBM33 cell line, which belongs to the GBM class and exhibits similar characteristics and functions as GBM cells. Compared to commercial cell lines, GBM33 cells show increased growth rate and similar sensitivity to temozolamide, and they can produce primary cilia upon serum starvation. The establishment of this cell line provides valuable tools for studying GBM biology, identifying therapeutic targets, and evaluating treatment efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Elena Piva, Paolo Fais, Pasquale Ioime, Mattia Forcato, Guido Viel, Giovanni Cecchetto, Jennifer P. Pascali
Summary: There is a global discussion on implementing safety limits for per- and polyfluoroalkyl substances (PFAS) in food due to their association with human diseases. Processed food is at higher risk of PFAS contamination due to manipulation and packaging. This study investigated the PFAS content in fresh, frozen, and ready-to-eat vegetables, showing significant differences in average PFAS content between the three groups. Organic vegetables displayed lower PFAS levels compared to conventional counterparts, suggesting possible contamination from pesticides use.
Article
Agriculture, Dairy & Animal Science
Amelia Jansen van Vuuren, Julie Bolcaen, Monique Engelbrecht, Willem Burger, Maryna De Kock, Marco Durante, Randall Fisher, Wilner Martinez-Lopez, Xanthene Miles, Farzana Rahiman, Walter Tinganelli, Charlot Vandevoorde
Summary: Preserving the genetic material of endangered wildlife species, such as the African savanna elephant, is crucial for future generations. Analyzing their genetic information helps us understand their adaptation and relationships, aiding in their conservation. The establishment of primary cell lines from elephant samples using a minimally invasive method allows for further research on their genetic material and cancer suppression ability.
Article
Cell Biology
Nicole Kiweler, Schwarz Helena, Alexandra Nguyen, Stephanie Matschos, Christina Mullins, Andrea Piee-Staffa, Christina Brachetti, Wynand P. Roos, Guenter Schneider, Michael Linnebacher, Walburgis Brenner, Oliver H. Kraemer
Summary: The expression of HDAC2 in colon cancer cells affects their response to the chemotherapy drug 5-FU and is associated with ATM-mediated sensitivity to 5-FU.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Biology
Falko Lange, Jakob Venus, Daria Shams Esfand Abady, Katrin Porath, Anne Einsle, Tina Sellmann, Valentin Neubert, Gesine Reichart, Michael Linnebacher, Ruediger Koehling, Timo Kirschstein
Summary: In electric fields, glioblastoma cells are capable of galvanotactic migration, and the PI3K/AKT pathway plays a critical role in this process.
Article
Oncology
Ulrike Pfohl, Juergen Loskutov, Sanum Bashir, Ralf Kuhn, Patrick Herter, Markus Templin, Soulafa Mamlouk, Sergei Belanov, Michael Linnebacher, Florian Buertin, Marcus Vetter, Christoph Reinhard, Lena Wedeken, Christian R. A. Regenbrecht
Summary: This research investigated the impact of loss-of-function of the SMAD4 gene on the sensitivity to MEK inhibitors in colorectal cancer using CRISPR technology. It identified a mutation signature related to the TGF-beta/BMP signaling pathway and found a gene signature (SFAB) that can predict sensitivity to MEK inhibitors.
Article
Biochemistry & Molecular Biology
Viktoriya B. Boncheva, Michael Linnebacher, Said Kdimati, Hannah Draper, Laurence Orchard, Ken Mills, Gerald O'Sullivan, Mark Tangney, Barbara-Ann Guinn
Summary: A Crohn's-like lymphoid reaction (CLR) is observed in about 15% of colorectal cancer (CRC) patients and is associated with favourable outcomes. By immunoscreening, the heavy chain of IgG3 (IGHG3) and a novel antigen named UOB-COL-7 were solely recognised by sera from CRC CLR patients. These findings demonstrate the potential of SEREX-identified CRC CLR antigens to act as biomarkers for CRC.
Article
Biochemistry & Molecular Biology
Randy Przybylla, Christina Susanne Mullins, Mathias Krohn, Stefan Oswald, Michael Linnebacher
Summary: This study established a platform of colorectal cancer patient-derived cell lines for evaluation of human intestinal drug absorption and metabolism, which could be superior tools for predicting oral drug absorption and intestinal drug-drug interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Julia Hoernschemeyer, Timo Kirschstein, Gesine Reichart, Christin Sasse, Jakob Venus, Anne Einsle, Katrin Porath, Michael Linnebacher, Ruediger Koehling, Falko Lange
Summary: Glioblastoma is a common and aggressive primary brain tumor with multiple genetic and epigenetic alterations. Small-molecule kinase inhibitors regorafenib and MK-2206, as well as dactolisib, showed effective results in all preclinical tumor models, while trametinib was found to be highly resistant.
Article
Oncology
Su Zhaoran, Christina Susanne Linnebacher, Michael Linnebacher
Summary: This study is the first to describe the significantly upregulated expression of the SEC23A gene in stomach adenocarcinoma (STAD). It also identified an association with disease progression, patients' prognosis, and infiltrating immune cell types and their activity. SEC23A is proposed as an independent prognostic factor with a putative role in immune response regulation in STAD.
Article
Oncology
Ramy Ashry, Al-Hassan M. Mustafa, Kristin Hausmann, Michael Linnebacher, Susanne Strand, Wolfgang Sippl, Matthias Wirth, Oliver H. Kraemer
Summary: Tumors in the pancreas and colon are still a clinical challenge. Different gene expression profiles between cancer cells and normal cells can be targeted by drugs that modulate protein acetylation. KH16 is a novel compound that induces hyperacetylation, leading to cell death in tumor cells while sparing normal cells. It shows superior efficacy compared to clinically established drugs. Further studies can focus on KH16 and similar compounds for cancer therapy.
Article
Cell Biology
Randy Przybylla, Mathias Krohn, Marie-Luise Sellin, Marcus Frank, Stefan Oswald, Michael Linnebacher
Summary: The commonly used in vitro model for ADME purposes, the Caco-2 cell line, has limitations in predicting intestinal drug absorption due to differences in gene and protein expression. This study evaluated low-passaged patient-derived colorectal cancer cell lines and found three lines with higher similarity to jejunal epithelial tissue cells and higher regulatory potential of drug transporters compared to the Caco-2 model.
Article
Biology
Falko Lange, Katrin Porath, Tina Sellmann, Anne Einsle, Robert Jaster, Michael Linnebacher, Ruediger Koehling, Timo Kirschstein
Summary: Migration of colorectal cancer cells greatly contributes to the progression of the disease. Direct-current electrical fields, known as galvanotaxis, could be a driving factor for directional migration. In this study, we investigated the migration of colorectal cancer cells in electrical fields and found that three out of five cell lines exhibited preferred cathodal migration. We determined that voltage-gated calcium channels and intracellular signaling pathways such as MEK and AKT played important roles in galvanotaxis.
Article
Biochemistry & Molecular Biology
Sandra Schwarz, Michael Linnebacher
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Meeting Abstract
Oncology
Christina Mullins, Sandra Wagner, Randy Przybylla, Florian Burtin, Michael Linnebacher
ONCOLOGY RESEARCH AND TREATMENT
(2022)
Article
Geriatrics & Gerontology
Lili Lu, Meng Dai, Christina Susanne Mullins, Clemens Schafmayer, Michael Linnebacher
Summary: This study evaluated the correlations between gastrointestinal (GI) cancers and Parkinson's disease (PD) using national cause-specific mortality data. The findings showed a global increase in mortality from PD, colorectal cancer (CRC), and pancreatic cancer (PC), while the mortality from esophagus cancer (EC), stomach cancer (SC), and liver cancer (LC) decreased. PD was found to have a positive correlation with LC and its associated subtypes, as well as a negative correlation with CRC and PC.
Article
Oncology
Anna M. Bielecka-Wajdman, Tomasz Ludyga, Daria Smyk, Wojciech Smyk, Magdalena Mularska, Patrycja Swiderek, Wojciech Majewski, Christina Susanne Mullins, Michael Linnebacher, Ewa Obuchowicz
Summary: The weakness of glucose metabolism is found to be related to the invasiveness and growth of GBM. DXM may support GBM energy metabolism and contribute to its recurrence. Under low oxygen and high glucose conditions, the study shows that DXM attenuates the cytotoxic effect of TMZ and intensifies malignancy features in GBM cell lines. A preliminary retrospective analysis indicates a correlation between serum glucose levels and Ki-67 expression in GBM patients.