Article
Biochemistry & Molecular Biology
Jesper Bergwik, Amanda Kristiansson, Charlotte Welinder, Olga Goransson, Stefan R. Hansson, Magnus Gram, Lena Erlandsson, Bo Akerstrom
Summary: Alpha(1)-microglobulin (A1M) is a ubiquitous protein with reductase and radical- and heme-binding properties, mainly expressed in the liver and encoded by the AMBP gene. Knockout mouse models lacking A1M expression but with preserved bikunin expression showed increased bikunin levels in the liver but decreased levels in plasma, as well as signs of increased ER stress and phenotypic changes such as increased body weight and lipid accumulation. The results suggest a role of A1M in regulating the intracellular redox environment, particularly in the liver.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Kira Dobrochaeva, Nailya Khasbiullina, Nadezhda Shilova, Nadezhda Antipova, Polina Obukhova, Tatiana Ovchinnikova, Oxana Galanina, Ola Blixt, Horst Kunz, Alexander Filatov, Yuriy Knirel, Jacques LePendu, Sergey Khaidukov, Nicolai Bovin
MOLECULAR IMMUNOLOGY
(2020)
Article
Oncology
Angela Barone, Anna Linder, Constantina Mateoiu, Rasmus Koster Larsen, Ola Blixt, Susann Teneberg, Karin Sundfeldt
FRONTIERS IN ONCOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Michel Rasmussen, Charlotte Welinder, Frank Schwede, Per Ekstrom
Summary: Studies have identified various cGMP-interacting proteins in the retina, which may help reduce photoreceptor cell death and serve as potential therapeutic targets or biomarkers for retinal degeneration.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Virology
Josanne H. Verhagen, Per Eriksson, Lonneke Leijten, Ola Blixt, Bjorn Olsen, Jonas Waldenstrom, Patrik Ellstrom, Thijs Kuiken
Summary: This study shows that Mallards play a significant role in the ecology and epidemiology of IAV, with abundant attachment to the colon and a preference for fucosylated glycan structures. Further understanding of virus tissue attachment and receptor binding is crucial for comprehending IAV host range and epidemiology.
JOURNAL OF VIROLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Daniel S. C. Butler, Caterina Cafaro, Johannes Putze, Murphy Lam Yim Wan, Thi Hien Tran, Ines Ambite, Shahram Ahmadi, Sven Kjellstrom, Charlotte Welinder, Sing Ming Chao, Ulrich Dobrindt, Catharina Svanborg
Summary: Bacterial protease can inhibit c-MYC protein in human cells, which leads to suppression of tumor cell proliferation and activity. This study provides a new direction and hope for targeted therapy of c-MYC.
NATURE BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Thomas Norberg, Elisabet Kallin, Ola Blixt
Summary: A simple method for attaching and detaching carbobenzyloxy (Cbz) groups at the reducing end of sugars was developed, facilitating the efficient preparation of specific oligosaccharide structures.
CARBOHYDRATE RESEARCH
(2021)
Article
Oncology
Lazaro Hiram Betancourt, Jeovanis Gil, Yonghyo Kim, Viktoria Doma, Ugur Cakir, Aniel Sanchez, Jimmy Rodriguez Murillo, Magdalena Kuras, Indira Pla Parada, Yutaka Sugihara, Roger Appelqvist, Elisabet Wieslander, Charlotte Welinder, Erika Velasquez, Natalia Pinto de Almeida, Nicole Woldmar, Matilda Marko-Varga, Krzysztof Pawlowski, Jonatan Eriksson, Beata Szeitz, Bo Baldetorp, Christian Ingvar, Hakan Olsson, Lotta Lundgren, Henrik Lindberg, Henriett Oskolas, Boram Lee, Ethan Berge, Marie Sjogren, Carina Eriksson, Dasol Kim, Ho Jeong Kwon, Beatrice Knudsen, Melinda Rezeli, Runyu Hong, Peter Horvatovich, Tasso Miliotis, Toshihide Nishimura, Harubumi Kato, Erik Steinfelder, Madalina Oppermann, Ken Miller, Francesco Florindi, Qimin Zhou, Gilberto B. Domont, Luciana Pizzatti, Fabio C. S. Nogueira, Peter Horvath, Leticia Szadai, Jozsef Timar, Sarolta Karpati, A. Marcell Szasz, Johan Malm, David Fenyo, Henrik Ekedahl, Istvan Balazs Nemeth, Gyorgy Marko-Varga
Summary: The MM500 study focuses on mapping protein levels in malignant melanoma tumor samples, identifying over 15,500 proteoforms and comparing the data with global proteomic experiments. This research contributes important information about melanoma to the NIH Cancer Moonshot initiative.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Lazaro Hiram Betancourt, Jeovanis Gil, Aniel Sanchez, Viktoria Doma, Magdalena Kuras, Jimmy Rodriguez Murillo, Erika Velasquez, Ugur Cakir, Yonghyo Kim, Yutaka Sugihara, Indira Pla Parada, Beata Szeitz, Roger Appelqvist, Elisabet Wieslander, Charlotte Welinder, Natalia Pinto de Almeida, Nicole Woldmar, Matilda Marko-Varga, Jonatan Eriksson, Krzysztof Pawlowski, Bo Baldetorp, Christian Ingvar, Hakan Olsson, Lotta Lundgren, Henrik Lindberg, Henriett Oskolas, Boram Lee, Ethan Berge, Marie Sjogren, Carina Eriksson, Dasol Kim, Ho Jeong Kwon, Beatrice Knudsen, Melinda Rezeli, Johan Malm, Runyu Hong, Peter Horvath, A. Marcell Szasz, Jozsef Timar, Sarolta Karpati, Peter Horvatovich, Tasso Miliotis, Toshihide Nishimura, Harubumi Kato, Erik Steinfelder, Madalina Oppermann, Ken Miller, Francesco Florindi, Quimin Zhou, Gilberto B. Domont, Luciana Pizzatti, Fabio C. S. Nogueira, Leticia Szadai, Istvan Balazs Nemeth, Henrik Ekedahl, David Fenyo, Gyorgy Marko-Varga
Summary: The MM500 meta-study aims to establish a knowledge basis of the tumor proteome as a complement to genome and transcriptome studies. By analyzing 505 well-annotated melanoma tumor samples, the study defined the melanoma proteome landscape, including thousands of proteins and mutated proteoforms. The study found a high degree of correlation between the melanoma transcriptome and proteome, increasing understanding of melanoma disease.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Felix Tobola, Martin Lepsik, Syeda Rehana Zia, Hakon Leffler, Ulf J. Nilsson, Ola Blixt, Anne Imberty, Birgit Wiltschi
Summary: This study introduced non-canonical tryptophan analogues into the ligand binding site of Galectin-1 and found two variants with reduced affinity for certain sugars. Through fluorescence polarization competition assay and molecular modeling, the researchers provided structural clues for the changes in affinity.
Article
Biochemistry & Molecular Biology
Michel Rasmussen, Charlotte Welinder, Frank Schwede, Per Ekstrom
Summary: This study investigated the stereospecific binding of cGMP analogs to cGMP-binding proteins, revealing crucial parameters for drug design and target specificity in drug development.
CHEMICAL BIOLOGY & DRUG DESIGN
(2022)
Article
Multidisciplinary Sciences
Madeleine Johansson, Hong Yan, Charlotte Welinder, Akos Vegvari, Viktor Hamrefors, Magnus Back, Richard Sutton, Artur Fedorowski
Summary: This study used proteomics to identify plasma protein biomarkers of postural orthostatic tachycardia syndrome (POTS) and revealed differentially regulated proteomic pathways in POTS. The findings suggest that POTS may be an autoimmune, inflammatory, and hyperadrenergic disorder, characterized by a hypercoagulable state, proinflammatory state, enhanced cardiac contractility and hypertrophy, skeletal muscle expression, and increased adrenergic activity.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Jiaming Zhou, Charlotte Welinder, Per Ekstroem
Summary: Retinitis pigmentosa (RP) is a common genetic disease that leads to the death of photoreceptor cells and blindness in industrial countries. Although gene therapy for certain mutations has been approved, there is currently no effective treatment available. This study explored the cGMP-PKG downstream signaling in degenerating retinas from a mouse model and identified potential substrates and kinases. Further investigation revealed the possible involvement of the RAS/RAF1/MAPK/ERK pathway in retinal degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Michel Rasmussen, Arianna Tolone, Francois Paquet-Durand, Charlotte Welinder, Frank Schwede, Per Ekstroem
Summary: RP is an inherited eye disease with an unknown cell death mechanism. Rp-8-Br-PET-cGMPS, a modified cGMP analog, selectively inhibits PKG and slows down photoreceptor death. Through affinity chromatography and mass spectrometry, it was found that Rp-8-Br-PET-cGMPS interacts with seven known cGMP-binding proteins and an additional 28 proteins. In summary, Rp-8-Br-PET-cGMPS is more target-specific compared to regular cGMP.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Pharmacology & Pharmacy
Komal Umashankar Rao, Ping Li, Charlotte Welinder, Erik Tenland, Pontus Gourdon, Erik Sturegard, James C. S. Ho, Gabriela Godaly
Summary: Multidrug-resistant tuberculosis (MDR) remains a threat to public health. A cationic host defense peptide has been identified with activity against M. tuberculosis and bactericidal effect against MDR M. tuberculosis. The peptide induces tube-shaped membranous structures, vesicle formation, and cell death in M. tuberculosis, and changes in secondary structure and aggregation in liposomes. It also targets essential proteins involved in mycolic acid synthesis and protein folding, impacting bacterial proliferation.