Article
Behavioral Sciences
Guihai Suo, Xing Cao, Yuqin Zheng, Haiying Li, Qi Zhang, Jihong Tang, Youjia Wu
Summary: Mutations in STXBP1 gene are associated with neurodevelopmental disorders and early-onset epileptic encephalopathies. Using a zebrafish model with mutant STXBP1, researchers demonstrated clinical phenotypes related to human STXBP1 mutation and evaluated antiepileptic drugs effects on seizures.
EPILEPSY & BEHAVIOR
(2021)
Article
Neurosciences
Ke-Liang Chen, Pei-Xi Li, Yi-Min Sun, Shu-Fen Chen, Chuan-Tao Zuo, Jian Wang, Qiang Dong, Mei Cui, Jin-Tai Yu
Summary: This article reports two cases of very early onset Alzheimer's disease (VEOAD) caused by de novo PSEN1 mutations. The findings increase the understanding of VEOAD genetics and expand the ethnic distribution of PSEN1 mutations.
JOURNAL OF ALZHEIMERS DISEASE
(2022)
Editorial Material
Clinical Neurology
Maike F. Dohrn, Adriana P. Rebelo, Siddharth Srivastava, Gerarda Cappuccio, Robert Smigiel, Alka Malhotra, Donald Basel, Ingrid van de Laar, Rinze Frederik Neuteboom, Coranne Aarts-Tesselaar, Sonal Mahida, Nicola Brunetti-Pierri, Ryan J. Taft, Stephan Zuchner
Summary: This study identified five unrelated children with intellectual disability, spasticity, and peripheral neuropathy carrying heterozygous ATP1A1 variants. The variants led to loss of ATPase function and were associated with additional symptoms such as sensory loss, sleep disturbances, and seizures. These de novo variants had high pathogenicity prediction scores and replicated the haploinsufficiency mechanism of disease.
Article
Oncology
Xiao-Wei Song, Ting Su, Bo Li, Yun-Jie Huang, Wen-Xia He, Li-Li Jiang, Chang-Jin Li, Song-Qun Huang, Song-Hua Li, Zhi-Fu Guo, Hong Wu, Bi-Li Zhang
Summary: In this study, the role of the lncRNA PRKAG2-AS in regulating the expression of PRKAG2 in cardiomyocytes was investigated. Knockdown of PRKAG2-AS in the nucleus significantly decreased the expression of PRKAG2b and PRKAG2d. PRKAG2-AS and its target genes were reduced in the hearts of patients with ischemic cardiomyopathy, suggesting a potential role in myocardial ischemia. Overexpression of PPARG, a interacting protein of PRKAG2-AS, led to an increase in PRKAG2b and PRKAG2d expression in cardiomyocytes, which could be attenuated by PRKAG2-AS knockdown. Additionally, PRKAG2-AS was elevated in hearts with dilated cardiomyopathy and its overexpression led to increased PRKAG2 expression, indicating its involvement in heart failure.
CLINICAL EPIGENETICS
(2023)
Article
Biochemistry & Molecular Biology
Chaowei Zhang, Kerry Reid, Arthur F. Sands, Antoine Fraimout, Mikkel Heide Schierup, Juha Merila
Summary: Mutation rate is a critical parameter in population genetics, but accurate estimates for wild organisms are limited. This study estimated mutation rates in two marine populations of nine-spined stickleback using pedigrees, whole-genome resequencing, and a high-quality reference genome. The results showed high levels of parental mosaicism and increased estimated divergence times after recalibration with the mutation rate.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Anna Orlova, Daria Guseva, Oxana Ryzhkova
Summary: This study described a new case of severe dilated cardiomyopathy caused by a frameshift mutation in the CASZ1 gene. The clinical presentation was similar to previous studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Pablo Aguero, Maria Jose Sainz, Raquel Tellez, Isabel Lorda, Almudena Avila, Guillermo Garcia-Ribas, Patricia Paredes Rodriguez, Estrella Gomez-Tortosa
Summary: We report a patient with sporadic Alzheimer's disease onset in his twenties carrying a de novo Pro436Gln mutation in the PS1 gene. The clinical phenotype includes posterior cortical syndrome and mild limb spasticity, with brain imaging showing severe parieto-occipital atrophy. Cerebrospinal fluid biomarkers indicate worsening condition.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Cardiac & Cardiovascular Systems
Michael Khoury, Jennifer Conway, Jeffrey G. Gossett, Erik Edens, Stephanie Soto, Ryan Cantor, Devin Koehl, Aliessa Barnes, Vernat Exil, Lauren Glass, James K. Kirklin, Warren A. Zuckerman
Summary: This study aimed to evaluate the association between the timing of cardiac allograft vasculopathy (CAV) occurrence post-heart transplant (HT) and graft survival and progression of CAV severity in pediatric HT recipients. The study found that later onset CAV (> 10 years post-HT) was associated with improved five-year graft survival compared to CAV onset at earlier time-points, but the long-term outcomes were similar and poor. The timing of CAV post-HT was not associated with progression of CAV disease severity.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2022)
Article
Cardiac & Cardiovascular Systems
Linda Sasset, Onorina Laura Manzo, Yi Zhang, Alice Marino, Luisa Rubinelli, Maria Antonietta Riemma, Madhavi Latha S. Chalasani, Dragos C. Dasoveanu, Fiorentina Roviezzo, Stanislovas S. Jankauskas, Gaetano Santulli, Maria Rosaria Bucci, Theresa T. Lu, Annarita Di Lorenzo
Summary: Nogo-A acts as a negative regulator of ceramide synthesis by inhibiting SPT activity, thereby limiting ceramide accumulation in cardiomyocytes and protecting against heart failure. Lack of Nogo-A leads to cardiac hypertrophy and dysfunction, ultimately resulting in the progression of heart failure.
CARDIOVASCULAR RESEARCH
(2023)
Article
Neurosciences
Jan Rusz, Tereza Tykalova, Michal Novotny, Evzen Ruzicka, Petr Dusek
Summary: The study compared patterns of speech disorder in drug-naive early-onset PD (EOPD) and late-onset PD (LOPD) patients, finding that despite similar perceptual dysarthria severity in both groups, EOPD showed weaker inspirations while LOPD had decreased voice quality and imprecise consonant articulation. Both PD subgroups exhibited common speech abnormalities such as monopitch, monoloudness, and articulatory decay, with worsening consonant articulation being correlated with the severity of axial gait symptoms. The distinct pattern of imprecise consonant articulation could be seen as an axial motor symptom of PD.
NPJ PARKINSONS DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Eugenio Lopez-Cortegano, Rory J. Craig, Jobran Chebib, Toby Samuels, Andrew D. Morgan, Susanne A. Kraemer, Katharina B. Boendel, Rob W. Ness, Nick Colegrave, Peter D. Keightley
Summary: De novo mutations play a crucial role in evolution by providing genetic variation, but studying them is challenging and often limited to model species, leading to a restricted understanding of mutation rate evolution among closely related species. Through a mutation accumulation experiment and comparative analysis between Chlamydomonas incerta and Chlamydomonas reinhardtii, researchers found variability in the mutation rate and differing mutation spectra, indicating similarities in genomic factors influencing mutation rate but a greater divergence in mutation spectra between the two species.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Cardiac & Cardiovascular Systems
Matthew Hoffman, Dimitra Palioura, Ioannis D. Kyriazis, Maria Cimini, Rachit Badolia, Sudarsan Rajan, Erhe Gao, Nikolas Nikolaidis, P. Christian Schulze, Ira J. Goldberg, Raj Kishore, Vincent W. Yang, Thomas D. Bannister, Agnieszka B. Bialkowska, Craig H. Selzman, Stavros G. Drakos, Konstantinos Drosatos
Summary: KLF5 is found to play a crucial role in the pathophysiology of ischemic heart failure, involving regulation of lipid metabolism. Genetic or pharmacological inhibition of KLF5 prevents ceramide accumulation, alleviates cardiac remodeling, and improves ejection fraction in mice with myocardial infarction.
Article
Genetics & Heredity
Juan Li, Shiyue Mei, Xiao Mao, Lily Wan, Hua Wang, Bo Xiao, Yanmin Song, Weiyue Gu, Yan Liu, Lili Long
Summary: This study identified de novo loss-of-function variants in the KCNJ3 gene associated with early-onset epilepsy. Genetic testing of KCNJ3 in patients with epilepsy may contribute to precision medicine.
JOURNAL OF MEDICAL GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Haoling Xie, Wen Li, Yuqiong Hu, Cheng Yang, Jiansen Lu, Yuqing Guo, Lu Wen, Fuchou Tang
Summary: This study achieved high continuity human genome assembly using single-cell genome long-read sequencing technology and explored the impact of different assemblers and sequencing strategies on genome assembly. It is of great significance for the practice of single-cell genome de novo assembly.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Genetics & Heredity
Chiara Kloeckner, Heinrich Sticht, Pia Zacher, Bernt Popp, Holly E. Babcock, Dewi P. Bakker, Katy Barwick, Michaela Bonfert, Carsten G. Bonnemann, Eva H. Brilstra, Wendy K. Chung, Angus J. Clarke, Patrick Devine, Sandra Donkervoort, Jamie L. Fraser, Jennifer Friedman, Alyssa Gates, Jamal Ghoumid, Emma Hobson, Gabriella Horvath, Jennifer Keller-Ramey, Boris Keren, Manju A. Kurian, Virgina Lee, Kathleen A. Leppig, Johan Lundgren, Marie T. McDonald, Amy McTague, Heather C. Mefford, Cyril Mignot, Mohamad A. Mikati, Caroline Nava, F. Lucy Raymond, Julian R. Sampson, Alba Sanchis-Juan, Vandana Shashi, Joseph T. C. Shieh, Marwan Shinawi, Anne Slavotinek, Tommy Stodberg, Nicholas Stong, Jennifer A. Sullivan, Ashley C. Taylor, Tomi L. Toler, Marie-Jose van den Boogaard, Saskia N. van der Crabben, Koen L. van Gassen, Richard H. van Jaarsveld, Jessica Van Ziffle, Alexandrea F. Wadley, Matias Wagner, Kristen Wigby, Saskia B. Wortmann, Yuri A. Zarate, Rikke S. Moller, Johannes R. Lemke, Konrad Platzer
Summary: This study provides a comprehensive description of SNAP25 developmental and epileptic encephalopathy (SNAP25-DEE) with intellectual disability and early-onset epilepsy as core symptoms, mostly presenting before the age of two. The findings suggest an overlap with other genes encoding components of the SNARE complex, advancing the concept of SNAREopathies as a group of neurodevelopmental disorders.
GENETICS IN MEDICINE
(2021)
Article
Medicine, General & Internal
Dan Hu, Dong Hu, Liwen Liu, Daniel Barr, Yang Liu, Norma Balderrabano-Saucedo, Bo Wang, Feng Zhu, Yumei Xue, Shulin Wu, BaoLiang Song, Heather McManus, Katherine Murphy, Katherine Loes, Arnon Adler, Lorenzo Monserrat, Charles Antzelevitch, Michael H. Gollob, Perry M. Elliott, Hector Barajas-Martinez
Article
Biochemistry & Molecular Biology
Jerome Clatot, Nathalie Neyroud, Robert Cox, Charlotte Souil, Jing Huang, Pascale Guicheney, Charles Antzelevitch
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Cardiac & Cardiovascular Systems
Can Hasdemir, Figen Gokcay, Mehmet N. Orman, Umut Kocabas, Serdar Payzin, Hatice Sahin, Dale R. Nyholt, Charles Antzelevitch
JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY
(2020)
Article
Chemistry, Physical
Luisa R. Garcia Michel, Clara D. Keirns, Benjamin C. Ahlbrecht, Daniel A. Barr
Summary: A new method for estimating transfer entropy in biomolecular systems was developed by analyzing the variance-covariance matrix of atomic fluctuations. The results showed systematic consistency of the method for calculating transfer entropy and provided insights into the interconnected residue networks in proteins. Additionally, evidence of possible allosteric activity and different paths of activation in mutants were presented, highlighting the important role of transfer entropy analysis in understanding allosteric behavior.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2021)
Article
Multidisciplinary Sciences
Ganxiao Chen, Xun Li, Zuojiong Gong, Hao Xia, Yao Wang, Xuefen Wang, Yan Huang, Hector Barajas-Martinez, Dan Hu
Summary: COVID-19 patients may experience cardiac injury and develop hypertension, with elevated levels of certain markers. Enhanced Ang II signaling from SARS-CoV-2 infection may contribute to the development of hypertension.
Article
Cardiac & Cardiovascular Systems
Alexander Burashnikov, Hector Barajas-Martinez, Robert Cox, Mark A. Demitrack, Michael J. Fossler, Michael Kramer, Robert B. Kleiman, Peter Kowey, Charles Antzelevitch
Summary: The study found that oliceridine and quinidine progressively prolonged the QTc interval and action potential duration in rabbit left ventricular wedge preparations over 5 hours, in line with slow tissue uptake of the drugs. Oliceridine caused modest prolongation of these parameters, while quinidine resulted in prominent prolongation and development of early afterdepolarization after 2 hours, leading to a high risk of torsades de pointes.
Article
Biochemistry & Molecular Biology
Yan Huang, Xiao-Meng Chen, Hector Barajas-Martinez, Hong Jiang, Charles Antzelevitch, Dan Hu
Summary: Genome-wide association studies have shown the significant association of SCN10A common variants with Brugada Syndrome susceptibility. Particularly, allele rs6795970 (V1073) was strongly associated with an increased risk for BrS. This allele exhibited electrophysiological changes contributing to BrS, including a positive shift in steady-state activation and slower recovery from inactivation.
HUMAN MOLECULAR GENETICS
(2022)
Letter
Cardiac & Cardiovascular Systems
Glauber M. Dias, Arsonval Lamounier Junior, Maila Seifert, Hector Barajas-Martinez, Daniel Barr, Eduardo B. Sternick, Enrique Medina-Acosta, Antonio C. Campos de Carvalho, Fernando E. S. Cruz Filho
CIRCULATION-GENOMIC AND PRECISION MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Dan Ye, Wei Zhou, Samantha K. Hamrick, David J. Tester, C. S. John Kim, Hector Barajas-Martinez, Dan Hu, John R. Giudicessi, Charles Antzelevitch, Michael J. Ackerman
Summary: Based on the findings, the researchers suggest that Acacetin may be a novel therapeutic for patients with KCND3 gain-of-function-associated J wave syndrome by inhibiting Ito and abolishing the accentuated action potential notch in patient-derived iPSC-CMs.
CIRCULATION-GENOMIC AND PRECISION MEDICINE
(2022)
Article
Biology
Bin Zeng, Xiang Zhang, Rainer Schimpf, Andrew Powers, Michael Glikson, Charles Antzelevitch, Dan Hu, Hector Barajas-Martinez
Summary: J wave syndrome (JWS) is a cardiac channelopathy associated with malignant ventricular arrhythmias and sudden cardiac death. This study explores the association between variants in the L-type calcium channel gene subunits, CACNA1C and CACNB2b, and the JWS phenotype. Through genetic sequencing, mutations in these genes were identified in individuals with JWS, suggesting a loss-of-function effect on the cardiac calcium channel current. These findings further support the hypothesis that variants in CACNA1C and CACNB2b are associated with JWS and should be included in genetic screening protocols.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Review
Biology
Dan Hu, Hector Barajas-Martinez, Zhong-He Zhang, Hong-Yi Duan, Qing-Yan Zhao, Ming-Wei Bao, Yi-Mei Du, Alexander Burashnikov, Michelle M. Monasky, Carlo Pappone, Cong-Xin Huang, Charles Antzelevitch, Hong Jiang
Summary: Atrial fibrillation (AF) is a common cardiac arrhythmia with a high prevalence worldwide. It is associated with an increased risk of death, stroke, and peripheral embolism. Despite genetic studies identifying AF-associated genes, their definitive impact is not yet established. This review discusses recent advances in understanding and managing AF, including epidemiology, genetics, pathophysiology, and potential treatment options.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jose M. Di Diego, Hector Barajas-Martinez, Robert Cox, Victoria M. M. Robinson, Joseph Jung, Mohamed Fouda, Mena Abdelsayed, Peter C. C. Ruben, Charles Antzelevitch
Summary: AR-787 has pleiotropic effects on cardiac ion channels and shows promise as a treatment for J wave syndromes and hypothermia.
Article
Nanoscience & Nanotechnology
Nam P. Vu, Luke Ali, Theresa L. Chua, Daniel A. Barr, Heidi P. Hendrickson, Dhara J. Trivedi
Summary: In this study, the interaction between the ligand Prostaglandin E2 and three GPCRs in the E-prostanoid family (EP1, EP2, and EP3) was investigated using long-time scale molecular dynamics simulations. Transfer entropy and betweenness centrality were used to measure the information transfer pathways among residues involved in ligand binding. The results provide insights into the activation and signal transduction pathways of these receptors and contribute to the development of potential therapeutics targeting them.
ACS APPLIED BIO MATERIALS
(2023)
Meeting Abstract
Biophysics
Luisa Garcia Michel, Clara Keirns, Daniel A. Barr
BIOPHYSICAL JOURNAL
(2021)