Article
Plant Sciences
Saeid Babaei, Mohan B. Singh, Prem L. Bhalla
Summary: This study reveals the expression and function of circular RNAs (circRNAs) in regulating floral development in soybean under short-day treatment. Through deep sequencing and computational analysis, 384 circRNAs were identified, with 129 showing short-day treatment-specific expression patterns. Several circRNAs with potential miRNA binding sites were also found, suggesting their involvement in regulating downstream gene expression through the circRNA-miRNA-mRNA network. Additionally, four different circRNAs with binding sites for important microRNA modules (miR156 and miR172) related to developmental phase transition in plants were identified. This study highlights the complexity of gene regulation during the transition from vegetative to reproductive growth in soybean and provides new insights into floral transition in crop plants.
Review
Plant Sciences
Qingqing Yang, Cunquan Yuan, Tianci Cong, Qixiang Zhang
Summary: The branching phenotype is a crucial agronomic trait of plants, with significance in both fruit trees' yield and the ornamental value of landscape trees and flowers. The regulation of meristem initiation by plant hormones and transcription factors plays a significant role in determining the branching characteristics. However, research on meristem initiation in horticultural plants is limited, and the mechanism of regulation is not well understood. This review summarizes recent progress in understanding the regulatory networks and mechanisms of axillary meristem and floral meristem initiation, and suggests the need for future studies to improve the branching trait in horticultural plants.
Article
Plant Sciences
Pengfei Hao, Baogang Lin, Yun Ren, Hao Hu, Bowen Xue, Lan Huang, Shuijin Hua
Summary: This study investigated the effects of different nitrogen application rates on the development stages of floral meristem differentiation. It was found that a high nitrogen rate can accelerate the initiation of floral meristem differentiation, with auxin playing an important regulatory role in this process.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Ying Wang, Yuling Jiao
Summary: Distinct from animals, plants maintain organogenesis from specialized tissues called meristems throughout life. The shoot apical meristem (SAM) in the shoot apex produces all aerial organs, and cell signaling within functional domains is crucial for SAM functions. Recent studies on the WUSCHEL-CLAVATA feedback loop and polar auxin transport and signaling have expanded our understanding of SAM homeostasis and organogenesis. Furthermore, single-cell techniques have improved our knowledge of cellular functions in the shoot apex.
Review
Biochemistry & Molecular Biology
Soeun Yang, Horim Lee
Summary: Salt stress has a severe impact on plant growth and development. Understanding the mechanisms related to growth and development in response to salt stress is crucial for plant tolerance and adaptation. This paper highlights the roles of microRNAs, redox status, reactive oxygen species, nitric oxide, and phytohormones in apical meristems for salt tolerance, as well as the relationship between salt stress response and stem cell homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Michael F. Schwartz, Rachel Peters, Aitch M. Hunt, Abdul-Khaliq Abdul-Matin, Lisa Van den Broeck, Rosangela Sozzani
Summary: In contrast to animals, plants continuously produce organs and structures through meristems. Plants have various types of meristems that must be tightly regulated to balance organ production and maintenance. In addition to summarizing plant meristems, this review also discusses cell types with similar molecular features but different maintenance rules.
CRITICAL REVIEWS IN PLANT SCIENCES
(2021)
Review
Plant Sciences
Filipa Lara Lopes, Carlos Galvan-Ampudia, Benoit Landrein
Summary: Recent studies have revealed the critical role of the feedback loop between WUS and CLV3 in maintaining the stem cell niche in the shoot apical meristem of plants. WUS promotes stem cell fate and is inhibited by CLV3, ensuring the stability of this niche. Furthermore, WUS can integrate both internal and external cues to enable the shoot meristem to adapt to various environmental changes.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Developmental Biology
Ya Min, Stephanie J. Conway, Elena M. Kramer
Summary: In-depth investigation of plant developmental processes requires understanding of molecular networks and cell division patterns. Using live imaging, this study explored the dynamics of floral organ initiation and meristem termination in Aquilegia coengea, revealing distinct patterns and providing crucial information for understanding the spatial-temporal regulation of floral meristem behavior.
Article
Plant Sciences
Hatsune Morinaka, Akihito Mamiya, Hiroaki Tamaki, Akitoshi Iwamoto, Takamasa Suzuki, Ayako Kawamura, Momoko Ikeuchi, Akira Iwase, Tetsuya Higashiyama, Keiko Sugimoto, Munetaka Sugiyama
Summary: This study characterized the direct shoot regeneration from epidermal cells in Torenia through cytological observation and transcriptome analyses, revealing three distinct phases with different transcriptomic and regulatory features. The research provides a basis for further investigation of shoot regeneration in this unique culture system.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hui Wang, Xue Li, Tezera Wolabu, Ziyao Wang, Ye Liu, Dimiru Tadesse, Naichong Chen, Aijiao Xu, Xiaojing Bi, Yunwei Zhang, Jianghua Chen, Million Tadege
Summary: The study reveals that activator WOX member NsWOX9 and repressor WOX member LAM1 regulate endogenous cytokinin balance during leaf blade outgrowth through a common target NsCKX3.
Review
Plant Sciences
Agata Burian
Summary: The plant shoot apical meristem (SAM) functions similarly to animal germline in reducing the accumulation and transmission of mutations through cellular- and tissue-level mechanisms.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Michela Osnato, Elia Lacchini, Alessandro Pilatone, Ludovico Dreni, Andrea Grioni, Matteo Chiara, David Horner, Soraya Pelaz, Martin M. Kater
Summary: Floral homeotic genes in angiosperms, such as OsMADS13 in rice, play a crucial role in determining ovule identity. The interaction between OsMADS13 and other genes, like those in the SEP family, may control a common set of target genes involved in ovule development. This study sheds light on the regulatory pathways in rice gynoecium development and provides insights into the role of OsMADS13 as a repressor of the carpel pathway during ovule development.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Agricultural Engineering
Mahesh Kumar Badhepuri, M. Manokari, M. Cokul Raj, Phanikanth Jogam, Abhijit Dey, Mohammad Faisal, Abdulrahman A. Alatar, Nirmal Joshee, Nageswara Rao Singisala, Mahipal S. Shekhawat
Summary: A novel and effective direct shoot regeneration technique was developed for the medicinal plant Coleus forskohlii. Meta-Topolin was found to be more efficient than BAP in promoting direct organogenesis. The regeneration process showed distinct phases of shoot formation. The developed protocol can be used for the commercial production of C. forskohlii.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Justyna Jadwiga Olas, Federico Apelt, Maria Grazia Annunziata, Sheeba John, Sarah Isabel Richard, Saurabh Gupta, Friedrich Kragler, Salma Balazadeh, Bernd Mueller-Roeber
Summary: The shoot apical meristem of Arabidopsis thaliana displays a memory of non-lethal heat stress, enabling it to recover growth after a lethal heat stress. RNA sequencing identified genes involved in establishing this heat stress transcriptional memory. Heat shock transcription factor A2 is essential for regulating the expression of memory genes in SAM.
Article
Biochemistry & Molecular Biology
Jie Wang, Qihang Jiang, Roman Pleskot, Peter Grones, Elmehdi Bahafid, Gregoire Denay, Carlos Galvan-Ampudia, Xiangyu Xu, Michael Vandorpe, Evelien Mylle, Ive De Smet, Teva Vernoux, Ruediger Simon, Moritz K. Nowack, Daniel Van Damme
Summary: Endocytosis is important for plant response to stimuli and TPC plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of TPC subunits cause severe phenotypes, but partially functional alleles cause mild deviations. Reduced TPC-dependent endocytosis leads to hypersensitivity to CLAVATA3 peptide signaling.
Article
Agronomy
Hina Arya, Mohan B. Singh, Prem L. Bhalla
Summary: The overexpression of GmPIF4b in soybean plants led to reduced plant height, leaf surface area, branching, early flowering, and faster transition from full-bloom flowering to full maturity stage. Additionally, changes were observed in pod color and seed hilum characteristics. These findings suggest that GmPIF4b could be a valuable gene target for soybean improvement programmes focusing on plant morphology and reproductive stages.
FOOD AND ENERGY SECURITY
(2021)
Article
Plant Sciences
Neeta Lohani, Saeid Babaei, Mohan B. Singh, Prem L. Bhalla
Summary: This study identified and classified the Dof genes of Brassica napus, revealing their evolutionary history and potential functional roles in plant development and responses to abiotic stress. The research provides a comprehensive understanding of the molecular structure, evolution, and possible functions of Dof genes in plant biology.
Article
Agronomy
Neeta Lohani, Mohan B. Singh, Prem L. Bhalla
Summary: This study reported the impact of short-term heat stress on oilseed rape. Short heat stress episodes, especially at temperatures above 36 degrees C, resulted in decreased reproductive fitness and reduced seed production. It also had negative effects on seed quality.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2022)
Article
Plant Sciences
Agnieszka A. Golicz, Annapurna D. Allu, Wei Li, Neeta Lohani, Mohan B. Singh, Prem L. Bhalla
Summary: Intron retention is a stage-specific mechanism that leads to functional attenuation of a subset of co-regulated, functionally related genes during early stages of pollen development. The modulation of protein levels in a plant developmental program is not induced by gene expression, but by the type of transcript produced. This mechanism may contribute to the stage-specific expression of a non-functional isoform of certain genes.
PLANT REPRODUCTION
(2021)
Review
Plant Sciences
Yanlin Shao, Chui Eng Wong, Lisha Shen, Hao Yu
Summary: RNA modifications, particularly m6A, play crucial roles in gene regulation in plants, impacting mRNA metabolism, development, and stress responses. Recent progress has been made in understanding the roles of m6A in plant biology, yet there are still many outstanding questions that need to be addressed in future research.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Saeid Babaei, Mohan B. Singh, Prem L. Bhalla
Summary: This study investigated the expression profile and potential function of circRNAs during pollen development in Brassica rapa through high-throughput RNA sequencing. Functional enrichment analysis revealed circRNAs' involvement in pollen-related molecular and biological processes, indicating their potential role in post-transcriptional gene regulation.Confirmation of back-splicing sites of selected circRNAs further supports the systematic analysis of circRNAs during pollen development for future studies on gene regulatory networks in flowering plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Tao Xu, Xiaowei Wu, Chui Eng Wong, Sheng Fan, Yu Zhang, Songyao Zhang, Zhe Liang, Hao Yu, Lisha Shen
Summary: The study discovers that FIO1 acts as a previously unknown m(6)A methyltransferase that mainly modifies the coding regions of Arabidopsis transcripts. FIO1-mediated m(6)A methylation determines the mRNA abundance of key regulatory genes, thereby regulating flowering time in plants.
Article
Plant Sciences
Neeta Lohani, Agnieszka A. A. Golicz, Annapurna D. D. Allu, Prem L. L. Bhalla, Mohan B. B. Singh
Summary: The genomic location and stage-specific expression pattern of many long non-coding RNAs reveal their critical role in regulating protein-coding genes crucial in pollen developmental progression and male germ line specification.
PLANT CELL REPORTS
(2023)
Article
Plant Sciences
Chui Eng Wong, Songyao Zhang, Tao Xu, Yu Zhang, Zhi Wei Norman Teo, An Yan, Lisha Shen, Hao Yu
Summary: This study systematically surveyed the quantitative m(6)A methylomes and corresponding transcriptomes in Arabidopsis. The m(6)A modification primarily promotes the usage of distal poly(A) sites and has little effect on RNA splicing. Impaired m(6)A writers result in hypomethylation and downregulation of transcripts encoding ribosomal proteins, suggesting a correlation between m(6)A and protein translation.
Review
Biochemistry & Molecular Biology
Roshira Colombage, Mohan B. Singh, Prem L. Bhalla
Summary: Increasing food demand and declining crop productivity due to climate change pose a threat to global food security. Developing improved crops that can withstand abiotic stresses is crucial. Melatonin, or phytomelatonin, in plants plays a vital role in alleviating oxidative damage and supporting plant survival under abiotic stress. Exogenous melatonin strengthens defense mechanisms by enhancing detoxification, promoting physiological activities, and upregulating stress-responsive genes. It also regulates plant hormones, activates stress-responsive genes, and improves protein homeostasis, resulting in increased plant survival under abiotic stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Sangil Lee, Mohan B. Singh, Prem L. Bhalla
Summary: Soybean requires short day photoperiod for flowering and microRNAs gma-miR156a and gma-miR172a play a crucial role in regulating soybean development. The study found that gma-miR156a is associated with juvenile development, while gma-miR172a is involved in reproductive development.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Shalini Tiwari, Mukesh Jain, Sneh Lata Singla-Pareek, Prem L. Bhalla, Mohan B. Singh, Ashwani Pareek
Summary: The study found that the salt-tolerant rice genotype Pokkali plays a crucial role in stress response through the regulation of long non-coding RNA (lncRNA). These lncRNAs modulate various cellular processes, including transcriptional regulation, ion homeostasis, and secondary metabolite production. Furthermore, lncRNAs are predicted to bind to stress-responsive transcription factors. Additionally, some lncRNAs are also regulated under other abiotic stresses and phytohormone treatments, indicating possible cross-talk and further functional characterization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)