Article
Medicine, Research & Experimental
Gregorio Guilherme Almeida, Inga Rimkute, Isabela Natalia Pascoal Campos Do Vale, Thomas Liechti, Priscilla Miranda Henriques, Ester Roffe, Fernanda Fortes de Araujo, Manoel Otavio da Costa Rocha, Silvana Maria Eloi Santos, Olindo Assis Martins-Filho, Dragana Jankovic, Alan Sher, Andrea Teixeira-Carvalho, Mario Roederer, Lis Ribeiro do Valle Antonelli
Summary: In this study, high-dimensional flow cytometry was used to evaluate CD4(+) T cells in patients with different clinical forms. The results showed that activated CD4(+) T cells were increased in patients, while functional regulatory T cells were reduced. These findings provide evidence for the involvement of these cells in the development of Chagas cardiomyopathy and their potential as biomarkers for disease progression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Y. Ana, J. D. Rojas Marquez, L. Fozzatti, R. E. Baigorri, C. Marin, B. A. Maletto, F. M. Cerban, R. Radi, L. Piacenza, C. C. Stempin
Summary: The study revealed that Trypanosoma cruzi infection triggers an exacerbated metabolism and mitochondrial oxidative stress in CD4 T cells, leading to an increased risk of cell apoptosis that may not be prevented by antioxidants.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Immunology
Gabriela Specker, Damian Estrada, Rafael Radi, Lucia Piacenza
Summary: This study demonstrates the protective effects of Trypanosoma cruzi mitochondrial peroxiredoxin (MPX) in macrophage infections and in response to nifurtimox (NFX) toxicity. MPX content increased after NFX treatment and overexpression of MPX enhanced resistance to the drug. MPX exhibits holdase activity and its overexpression was observed in temperature-challenged parasites. These findings suggest a protective role of MPX holdase activity against NFX toxicity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Oscar J. Cordero, Carlos Rafael-Vidal, Ruben Varela-Calvino, Cristina Calvino-Sampedro, Beatriz Malvar-Fernandez, Samuel Garcia, Juan E. Vinuela, Jose M. Pego-Reigosa
Summary: Immune system CD4 T-cells with high cell-surface CD26 expression demonstrate anti-tumoral properties, especially when engineered with a chimeric antigen receptor. Different T helper cell subsets show variations in CD26 expression levels, which could impact research on CAR-T cells. The relationship between glycoprotein sCD26 and its enzymatic activity, as well as its correlation with specific T cell subsets, still requires further understanding.
Article
Immunology
Livia Silva Araujo Passos, Carolina Cattoni Koh, Luisa Mourao Dias Magalhaes, Maria do Carmo Pereira Nunes, Kenneth John Gollob, Walderez Ornelas Dutra
Summary: DN T cells play a critical role in the cytokine network associated with Chagas heart disease, with IND patients displaying more central memory cells and CARD patients having more effector memory cells. Central memory DN T cells in IND patients show a balanced cytokine profile, while effector memory DN T cells in CARD patients do not. Targeting the activation of effector memory DN T cells may be a potential strategy to control inflammation in Chagas cardiomyopathy and other inflammatory diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Luciana L. Soprano, Maximiliano R. Ferrero, Malena Landoni, Gabriela A. Garcia, Monica I. Esteva, Alicia S. Couto, Vilma G. Duschak
Summary: This study evaluated the effects of GlcNAc6S on T. cruzi infection using a mouse experimental model. The findings showed that sulfotopes and their specific antibodies play a crucial role in cardiac tissue damage and T. cruzi infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Fatima Ferragut, Karen M. Cruz, Juan P. Gallardo, Marisa Fernandez, Yolanda Hernandez Vasquez, Karina A. Gomez
Summary: This study focuses on T-cell assays that define the parasite-specificity based on upregulation of TCR stimulation-induced surface markers. The results demonstrate the efficacy of activation-induced markers (AIM) assays in detecting T. cruzi-specific CD4(+) and CD8(+) T cells in chronic Chagas disease patients, using different combinations of surface markers. Additionally, this approach is also successful in identifying the phenotype of activated T lymphocytes based on the expression of CD45RA and CCR7.
Article
Immunology
Nadjania Saraiva de Lira Silva, Cristina Mary Orikaza, Fabiana Rodrigues de Santana, Luana Aguiar dos Santos, Bruno Ramos Salu, Maria Luiza Vilela Oliva, Rita de Cassia Sinigaglia, Renato Arruda Mortara
Summary: IL-9 plays a role in controlling T. cruzi infection by reducing invasion and multiplication in vitro, and acts as a protective factor during the chronic phase of experimental infection in BALB/c mice, controlling cardiac fibrosis and proinflammatory cytokine production.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Jose L. Saenz-Garcia, Beatriz S. Borges, Normanda Souza-Melo, Luiz V. Machado, Juliana S. Miranda, Lisandro Alfonso Pacheco-Lugo, Nilmar S. Moretti, Richard Wheleer, Lia C. Soares Medeiros, Wanderson D. DaRocha
Summary: The flagellum of Trypanosomatids contributes to multiple functions, and this study explores the role of Trypanin in T. cruzi. The deletion of Trypanin affects the growth and motility of T. cruzi epimastigotes, as well as their infection capacity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Carlos-Henrique D. Barbosa, Fabio B. Canto, Ariel Gomes, Layza M. Brandao, Jessica R. Lima, Guilherme A. Melo, Alessandra Granato, Eula G. A. Neves, Walderez O. Dutra, Ana-Carolina Oliveira, Alberto Nobrega, Maria Bellio
Summary: CD4CTLs play an important role in different pathologies, but the mechanisms controlling their generation are poorly understood. In this study, CD4CTLs were found to abundantly differentiate during mouse infection with Trypanosoma cruzi, exhibiting specific cytotoxicity and expressing immunoregulatory and exhaustion markers. The magnitude of the CD4CTL response was found to be controlled by T-cell intrinsic IL-18R/MyD88 signaling.
Article
Immunology
Di Wang, Yu Jiang, Yangzi Song, Yongqin Zeng, Cuilin Li, Xinyue Wang, Ying Liu, Jiang Xiao, Yaxian Kong, Hongxin Zhao
Summary: The study revealed that CD4(+) T cells in PLHIV exhibited enhanced secretion of proinflammatory cytokines and polyfunctionality due to HIV disease progression, while altered T-cell subsets led to discordant results in total and CD4(+) T-cell subsets.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Immunology
Rosa Isela Galvez, Thomas Jacobs
Summary: Infection with Trypanosoma cruzi is the most neglected zoonosis in Latin America. CD8(+) T cells play a crucial role in controlling the infection, but cannot eliminate all parasites. Chronic infection leads to dysfunctional CD8(+) T cells with high expression of inhibitory receptor PD-1 and co-expression of transcription factor TOX.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Naoya Tatsumi, Yosuke Kumamoto
Summary: Conventional dendritic cells (cDCs) are a powerful type of antigen-presenting cells, consisting of different subsets with distinct developmental, phenotypic, and functional characteristics. After immunization, each subset of cDCs acquires antigens in the secondary lymphoid organs and presents them to CD4T cells in a spatiotemporal manner, resulting in multiple waves of antigen presentation. This review focuses on the kinetics of antigen presentation by each cDC subset and its impact on priming naive CD4T cells, as well as its implications in CD4T cell differentiation.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Virology
Xiaolei Wang, Widade Ziani, Ronald S. Veazey, Huanbin Xu
Summary: The size of the HIV reservoir in CD4+ T cells during primary infection remains unknown. Memory CD4+ T cells appear to be the major targets for primary infection, and viral reservoirs are equally distributed in systemic and lymphoid compartments in acutely SIV-infected macaques.
Article
Immunology
Natalia Ivanova, Ana Luisa Junqueira Leite, Marcel Barbosa Vieira, Otto Henrique Cezar e Silva, Ludmilla Walter Reis Mota, Guilherme de Paula Costa, Cristiano Schetini de Azevedo, Sarah Alves Auharek, Romulo Dias Novaes, Kelerson Mauro de Castro Pinto, Rodrigo Fernando Bianchi, Andre Talvani
Summary: The study suggests that blue light therapy may be an effective strategy in controlling Trypanosoma cruzi by reducing parasite replication, decreasing parasite count in blood and cardiac tissue, and reducing volume density of cardiac connective tissue. The therapy also led to decreased levels of pro-inflammatory cytokines in infected animals. More research is needed to fully explore the potential of this non-chemical treatment approach for Chagas disease.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cristina Cacho-Navas, Natalia Reglero-Real, Natalia Colas-Algora, Susana Barroso, Gema de Rivas, Kostantinos Stamatakis, Jorge Feito, German Andres, Manuel Fresno, Leonor Kremer, Isabel Correas, Miguel A. Alonso, Jaime Millan
Summary: In this study, the researchers investigate the mechanisms of ICAM-1 localization and its impact on lymphocyte-hepatic epithelial cell interaction. They discover that plasmolipin plays a crucial role in ICAM-1 polarization and its absence impairs ICAM-1 transcytosis, leading to increased T-cell adhesion to hepatic epithelial cells.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Oncology
Javier Galan-Martinez, Konstantinos Stamatakis, Ines Sanchez-Gomez, Silvia Vazquez-Cuesta, Nuria Girones, Manuel Fresno
Summary: TCFL5 plays an important role in the development of colorectal cancer (CRC), with its functions depending on specific isoforms. Different isoforms of TCFL5 have distinct effects on proliferation, migration, and spheroids formation in CRC cell lines. This study highlights the importance of analyzing TCFL5 isoforms separately in cancer research.
MOLECULAR ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Konstantinos Stamatakis, Patricia Torres-Gerica, Alba Jimenez-Segovia, Edurne Ramos-Munoz, Lorena Crespo-Toro, Patricia Fuentes, Maria L. Toribio, Francisco Callejas-Hernandez, Alfredo Carrato, Maria Laura Garcia Bermejo, Manuel Fresno
Summary: COX2-regulated genes were studied in a mouse colorectal cancer cell line and circulating tumor cells (CTCs) were identified using a cell separation system. Gene expression analysis in different samples showed differences between healthy and tumor-bearing mice, which could provide valuable diagnostic and prognostic information.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Sara Francisco, Jean-Marc Billod, Javier Merino, Carmen Punzon, Alicia Gallego, Alicia Arranz, Sonsoles Martin-Santamaria, Manuel Fresno
Summary: This study reveals that Ochrobactrum intermedium LPS can activate both TLR4 and TLR2, indicating the occurrence of TLR4/TLR2 heterodimerization, and the core saccharide plays an important role in this interaction.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Maria del Carmen Maza, Maria Ubeda, Pilar Delgado, Lydia Horndler, Miguel A. Llamas, Hisse M. van Santen, Balbino Alarcon, David Abia, Laura Garcia-Bermejo, Sergio Serrano-Villar, Ugo Bastolla, Manuel Fresno
Summary: This study found that highly exposed but uninfected individuals have significantly higher levels of ACE2 in their serum. Furthermore, the serum from these seronegative individuals can effectively neutralize SARS-CoV-2 infection, suggesting that high levels of ACE2 may provide some protection against active infection without generating a conventional antibody response. Additionally, the study also revealed that ACE2 levels were higher in infected patients who developed cutaneous symptoms compared to respiratory symptoms, and ACE2 levels were higher in patients with milder symptoms.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Javier Galan-Martinez, Ines Berenguer, Ma del Carmen Maza, Konstantinos Stamatakis, Nuria Girones, Manuel Fresno
Summary: TCFL5 plays a crucial role in spermatogenesis by ensuring the progression of meiosis and the maturation of sperm cells.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Cristina Cacheiro-Llaguno, Elena Hernandez-Subira, Manuel D. Diaz-Munoz, Manuel Fresno, Juan M. Serrador, Miguel A. Iniguez
Summary: This research investigates the inhibitory effect of dexamethasone on COX-2 transcriptional induction during T cell activation, revealing the immunomodulatory properties of glucocorticoids in human T cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lucia Perez-Regidor, Joan Guzman-Caldentey, Nils Oberhauser, Carmen Punzon, Balazs Balogh, Jose R. Pedro, Eva Falomir, Alessandra Nurisso, Peter Matyus, J. Carlos Menendez, Belen de Andres, Manuel Fresno, Sonsoles Martin-Santamaria
Summary: In this study, a computer-aided drug repurposing approach was used to virtually screen and discover novel TLR4 modulators with a non-lipopolysaccharide-like structure. After screening thousands of approved drugs and drug-like molecules, several compounds with TLR4 antagonist activity were identified through biological assays. This collaborative work expands the chemical diversity for the inspiration of new classes of TLR4 modulators.
Article
Biochemistry & Molecular Biology
Natalia Cuesta, Sonia Fernandez-Veledo, Carmen Punzon, Cristobal Moreno, Beatriz Barrocal, Vinatha Sreeramkumar, Manuel Desco, Manuel Fresno
Summary: Understanding the signaling cascades that govern adipocyte metabolism and differentiation is crucial for developing obesity therapies. This study found that TLR2 and TLR4 have opposing roles in white adipose tissue, indicating cross-regulation between the two receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)