Article
Agriculture, Dairy & Animal Science
Sinyong Jeong, Namchul Jo, Jung-Jin Lee, Jae-Hwan Lee, Dong-Keun Kam, Jakyeom Seo, Ermias Kebreab, Seongwon Seo
Summary: This study aimed to evaluate the effects of dietary supplementation with bacteriophage and beta-mannanase on health and growth performance in calves. The results indicated that bacteriophage supplementation had a positive effect on calf survival rate, while beta-mannanase supplementation improved growth performance in calves.
Article
Biochemistry & Molecular Biology
Wenting Liu, Cuiping Ma, Weidong Liu, Yingying Zheng, Chun-Chi Chen, Ailing Liang, Xuegang Luo, Zhongyuan Li, Wenjian Ma, Yajian Song, Rey-Ting Guo, Tongcun Zhang
Summary: This study presents a novel GH113 beta-mannanase, BaMan113A, with substrate preference towards manno-oligosaccharides. Mutagenesis successfully increased its activity and altered the product distribution towards polysaccharides. The structural features and engineering strategies for enhancing the endo-acting activity of BaMan113A were also discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Fengzhen Zheng, Abdul Basit, Jiaqiang Wang, Huan Zhuang, Jun Chen, Jianfen Zhang
Summary: In this study, a acidophilic GH5 beta-mannanase with high activity and tolerance to acidic environments was efficiently expressed in Pichia pastoris. The enzyme showed specificity towards locust bean gum and exhibited optimal activity at pH 4.0 and 65 degrees C. Site-directed mutagenesis confirmed the crucial catalytic residues of the enzyme. Additionally, a synergistic effect was observed when the enzyme was combined with a commercial alpha-galactosidase.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Samuel J. Butler, Simon Birgersson, Mathias Wiemann, Monica Arcos-Hernandez, Henrik Stalbrand
Summary: The study demonstrated that the mutant R171K/E205D had a twofold higher propensity for transglycosylation with mannotetraose and allyl alcohol compared to wild-type TrMan5A. By employing enzyme synergy with GH27 guar alpha-galactosidase, there was a 2.1-fold increase in allyl mannosides and significant production of allyl galactopyranoside, leading to a 4.4-fold increase in overall yield of allyl glycosides.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yonca Yuzugullu Karakus, Binnur Yildirim, Arda Acemi
Summary: Fennel seeds were identified as a promising source of polyphenol oxidase (PPO) and the purified PPO enzyme was characterized in detail for the first time. The enzyme showed the best activity towards the substrate catechol, suggesting potential industrial applications in producing low-cost enzymes. Inhibitors and organic solvents were found to impact PPO activity, with certain solvents maintaining the enzyme's activity at high concentrations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Bowen Yan, Yuheng Tao, Caoxing Huang, Chenhuan Lai, Qiang Yong
Summary: This study investigated the production of low molecular weight galactomannan (LMW-GM) from leguminous seeds using enzymatic hydrolysis. The researchers used a one-pot fermentation technology to cultivate Trichoderma reesei with a cocktail of beta-mannanase and alpha-galactosidase. The results showed that a combination of avicel and melibiose as carbon sources resulted in an enzyme cocktail with high activities of beta-mannanase and alpha-galactosidase. The use of melibiose effectively induced the production of alpha-galactosidase, which improved the degradation of galactose in GM and increased the yield of LMW-GM.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Lei Liu, Yanchao Sun, Pengcheng Di, Yakun Cui, Qingchang Meng, Xiaming Wu, Yanping Chen, Jianhua Yuan
Summary: The maize brassinosteroid-signaling kinase gene ZmBSK1 plays a significant role in the response to salt stress. Overexpression of ZmBSK1 improves salt tolerance in maize by reducing cellular damage and oxidative stress and modulating the expression of antioxidant defense enzymes and proline biosynthesis-related genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Gaurav Singh Kaira, Mukesh Kapoor
Summary: This review provides a comprehensive overview of research on endo-beta-mannanase engineering, covering key factors such as activity stability, substrate recognition, and mode of action, as well as rational and directed evolution approaches to improve industrial biocatalysts.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Soil Science
Shicheng Zhao, Fenliang Fan, Shaojun Qiu, Xinpeng Xu, Ping He, Ignacio A. Ciampitti
Summary: This study reveals the importance of fungal abundance in residue decomposition, with the composition of fungal community shifting from copiotrophic populations dominating in the early stage to oligotrophic populations in the later stage.
APPLIED SOIL ECOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Jinyun Gu, Dandan Wang, Qian Wang, Weiming Liu, Xiaoyi Chen, Xianzhen Li, Fan Yang
Summary: This study reports the properties of a xanthan-degrading enzyme called Mibgl3, isolated from Microbacterium sp. XT11. Mibgl3 shows typical structural features of the GH3 family, but has low sequence identity with other GH3 enzymes. It exhibits a wide pH range tolerance and can be activated by high concentrations of NaCI, making it commercially valuable. Mibgl3 has higher substrate specificity for oligoxanthan and can effectively hydrolyze it to release glucose and glucuronate. This knowledge of Mibgl3's enzymatic properties and hydrolysis mechanism will be beneficial for future applications.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Iththichai Chimpanao, Suwattana Pruksasri
Summary: The beta-mannanase present in the commercial enzyme Pectinex Ultra SP-L exhibited efficient activity in hydrolyzing copra meal to produce mannooligosaccharides (MOS). Optimum mannanase activity was observed at pH 4.0 and a temperature of 65 degrees C. The hydrolysate obtained from the hydrolysis of copra meal using Pectinex Ultra SP-L contained approximately 45% oligosaccharides, with mannobiose being the predominant component. This study presents a simple and efficient method for obtaining oligosaccharides from copra meal using a commercial pectinolytic enzyme.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Carlos Murguiondo, Anna Mestre, Juan A. Mendez-Liter, Manuel Nieto-Dominguez, Laura de Eugenio, Maria Molina-Gutierrez, Maria Jesus Martinez, Alicia Prieto
Summary: The recombinant beta-xylosidase (rBxTW1) and its mutant, immobilized as magnetic cross-linked enzyme aggregates (mCLEAs), maintained or improved their catalytic properties, including the synthesis of various xylosides. The mCLEAs showed recyclability and improved thermal and pH stability, making them suitable for multiple cycles of synthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Agronomy
Hong Ren, Hua Qi, Ming Zhao, Wenbin Zhou, Xinbing Wang, Xiangwei Gong, Ying Jiang, Congfeng Li
Summary: In this study, it was found that under higher plant density, compared to ZD958, XY335 exhibited higher grain yield, KNP, and sink capacity mainly due to its higher net photosynthetic rate and sucrose synthase activities in grains.
Article
Agriculture, Dairy & Animal Science
Na Li, Yang Liu, Changyu Wang, Peifang Weng, Zufang Wu, Yazhu Zhu
Summary: The novel galactosidase Gal3149 from Bacillus velezensis SW5 showed higher enzyme activity for oNPG than pNPαG, optimal activity at pH 8.0 and 50°C, and good thermal stability. It was also significantly improved by K+ and Na+, but inhibited by several substances. Gal3149 has potential applications in lactose-reduced dairy industry.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Juan Hilario Cafiero, Maria Carla Martini, Mauricio Javier Lozano, Carolina Vacca, Antonio Lagares, Pablo Emiliano Tomatis, Maria Florencia Del Papa
Summary: This study characterized a novel subclass B2 MBL called BioF identified in an environmental Pseudomonas sp. strain, which functions as a potent carbapenemase with incipient activity against some cephalosporins. The BioF activity is not affected by excess zinc but is only inhibited at high metal chelator concentrations, highlighting the difficulty in suppressing B2 carbapenemase activity.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Immunology
Haitao Ren, Dachun Zhao, Xiaolu Xu, Yingmai Yang, Siyuan Fan, Wenhan Li, Hongzhi Guan
Summary: Anti-PKC gamma antibodies are rare onconeural antibodies associated with paraneoplastic cerebellar degeneration (PCD), with only a few reported cases. This study presents a Chinese patient with PCD and anti-PKC gamma antibodies, whose symptoms gradually improved after radiotherapy and intravenous immunoglobulin therapy for lymphoepithelial carcinoma found in the tonsil.
JOURNAL OF NEUROIMMUNOLOGY
(2021)
Article
Developmental Biology
Mark Perfetto, Xiaolu Xu, Congyu Lu, Yu Shi, Natasha Yousaf, Jiejing Li, Yvette Y. Yien, Shuo Wei
Summary: Mutations in the DDX3 gene may cause intellectual disabilities in humans, affecting neural crest development and craniofacial morphogenesis by regulating AKT kinase activity. This suggests a conserved role of DDX3 in neural crest development and provides insights into potential mechanisms for birth defects associated with DDX3 mutations.
Article
Electrochemistry
Meng Zhang, Chunxiang Lu, Zhihong Bi, Xiaolu Xu, Xiaodan Ren, Xinzhi Li, Kuan Lu, Shuxia Yuan
Summary: PNCA, a cellulose-based highly pyrrolic-nitrogen-doped carbon aerogel, exhibits excellent electrochemical performance as a cathode material in lithium-sulfur batteries. The study sheds light on the potential and future applications of nitrogen-doped carbon materials in lithium-sulfur batteries.
Article
Engineering, Multidisciplinary
Xiao-Lu Xu, Shun-Xin Li, Ying Yang, Xiang-Chao Sun, Hong Xia
Summary: This study presents a simple and high-performance flexible strain sensor based on gold nanoparticles and a polyimide substrate. The sensor demonstrates sensitive detection of subtle strains, excellent linearity, low power consumption, and the ability to adhere to human skin for physiological parameter monitoring. It can accurately detect the angles of cervical vertebrae in different bending states and distinguish artery pulse waveforms under various conditions and in different populations.
Article
Obstetrics & Gynecology
Fangxu Lin, Caihong Chen, Mengying Li, Hao Shi, Xiaolu Xu, Xue Jiang, Yu Liu, Jun Zhai, Yihong Guo
Summary: Improving uterine cavity abnormalities has little impact on the pregnancy outcomes of patients undergoing IVF/ICSI-ET, but the miscarriage rate is higher in the complex uterine cavity abnormality group. Female partner's age and history of intrauterine adhesions are independent risk factors for miscarriage.
JOURNAL OF MINIMALLY INVASIVE GYNECOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaolu Xu, Sabine Hummel, Klaus Harter, Uener Kolukisaoglu, Michael Riemann, Peter Nick
Summary: The transition to terrestrial plants led to a loss of microtubule minus-end-directed dynein motors and an expansion of class-XIV kinesins. OsDLK, a class-XIV kinesin from rice, plays a role in cell elongation during development and moves into the nucleus in response to cold stress. The import of OsDLK into the nucleus might be correlated with its specific DNA binding, particularly to the Opaque2 motif, which is found in the promoter region of NtAvr9/Cf9, a transcription factor involved in cold adaptation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Weijiang Huang, Kui Wang, Chunyun Tu, Xiaolu Xu, Qin Tian, Chao Ma, Qiuping Fu, Wei Yan
Summary: This study investigated the synergistic effects of PN-DOPO and OMMT on the flame retardancy, thermal stability, and mechanical properties of PP composites. The results showed that the combination of PN-DOPO and OMMT improved the fire resistance and overall performance of the PP composites. Rheological and TGA analysis indicated enhanced network structure and thermal stability in the presence of OMMT.
Article
Genetics & Heredity
Yin Lu, Qing Ma, Xiaolu Xu, Zhimin Wang, Ivanets Andrie, Tatsiana Savitskaya, Seyit Yuzuak
Summary: This study characterized the complete chloroplast genome of Elaeagnus pungens, an evergreen shrub with high medicinal values. The chloroplast genome of E. pungens is 152,218 bp in length and contains 113 unique genes. Phylogenomic analysis revealed that E. pungens forms a sister clade with species from Elaeagnus.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Clinical Neurology
Ren Wei, Xiaolu Xu, Yunyun Duan, Ningnannan Zhang, Jie Sun, Haiqing Li, Yuxin Li, Yongmei Li, Chun Zeng, Xuemei Han, Fuqing Zhou, Muhua Huang, Runzhi Li, Zhizheng Zhuo, Frederik Barkhof, James H. Cole, Yaou Liu
Summary: This study evaluated the clinical significance of deep learning-derived brain age prediction in NMOSD and RRMS. The results showed that both NMOSD and RRMS patients had a significantly higher brain age gap compared to healthy controls. The brain age gap was associated with baseline disability score, advanced brain volume loss, and disease duration. Additionally, the brain age gap predicted the worsening of disability status in both NMOSD and RRMS patients.
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2023)
Article
Oncology
Ting Sun, Yongzhi Wang, Xing Liu, Zhaohui Li, Jie Zhang, Jing Lu, Liying Qu, Sven Haller, Yunyun Duan, Zhizheng Zhuo, Dan Cheng, Xiaolu Xu, Wenqing Jia, Yaou Liu
Summary: This study aimed to develop a fully automated deep learning pipeline for the stratification of overall survival in spinal cord astrocytoma patients based on preoperative MR images. A total of 587 patients were included to develop an automated pipeline for tumor segmentation and survival prediction. The results showed that the automated models achieved accurate predictions of 1-year, 3-year, and 5-year survival rates.
Article
Endocrinology & Metabolism
Menghui Zhang, Xiaocong Liu, Xiaolu Xu, Jing Li, Zhiqin Bu, Qingling Yang, Hao Shi, Wenbin Niu, Shanjun Dai, Yuling Liang, Yihong Guo
Summary: This study aimed to evaluate the cut-off value of anti-Mullerian hormone (AMH) combined with body mass index (BMI) in the diagnosis of polycystic ovary syndrome (PCOS) and polycystic ovary morphology (PCOM). The results showed that AMH is an independent predictor of PCOS and PCOM. The distribution of AMH in PCOS was significantly different based on different phenotypes and severity of ovulation disorders.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Engineering, Chemical
Markus Noeske, Jannes Mueller, Christine Nowak, Kangqi Li, Xiaolu Xu, Sandra Breitung-Faes, Arno Kwade
Summary: This study presents a targeted comminution approach for component mixtures in a wet-operated stirred media mill. The researchers developed a process for producing battery slurries with fine ground silicon particles, carbon black, and graphite particles. By analyzing the particle size distributions of the carbon black and graphite particles separately, the slurry preparation process was transferred from a complex multistage batch process to a continuous one-passage mode. The prepared slurries were further processed into silicon-rich anodes and tested in full cells, showing comparable electrochemical behavior to anodes produced using a dissolver or mill-based slurry production process.
Proceedings Paper
Automation & Control Systems
Jianjun Yang, Xiaolu Xu, Xiaohui Yin, Wenxing Liu, Yi Yuan, Jian Peng
Summary: Soft sensing focuses on real-time prediction of key performance indicators or quality variables in production processes. Stacked autoencoders help learn high-level data features, while gated stacked isomorphic autoencoders integrate information from different hidden layers for prediction and estimation.
2022 41ST CHINESE CONTROL CONFERENCE (CCC)
(2022)
Meeting Abstract
Oncology
Zibin Liang, Xiaolu Xu, Shuanshuan Guo, Xiaoli Niu, Lubin Yan, Yanfei Zou, Zhong Lin, Peijian Peng, Zhihui Wang, Liyang Hu
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Xiaolu Xu, Yueheng Li, Zhengyan Yang, Zhi Zhou
Summary: This study found that TRPV1 regulates periodontitis through the PI3K/AKT signaling pathway, and the TRPV1 agonist and antagonist have different effects on the rat periodontitis model.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2022)
