Article
Biochemistry & Molecular Biology
Kevin E. W. Namitz, Song Tan, Michael S. Cosgrove
Summary: In this study, the biophysical properties of a reconstituted human MLL1 core complex were characterized. It was found that the MLL1-WDR5 heterodimer interacts with the RbBP5-Ash2L-DPY30 subcomplex in a hierarchical assembly pathway that is highly dependent on concentration and temperature. Surprisingly, the disassembled state is favored at physiological temperature, leading to irreversible inactivation of the enzyme.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biology
Imke Ensinck, Alexander Maman, Waleed S. Albihlal, Michelangelo Lassandro, Giulia Salzano, Theodora Sideri, Steven A. Howell, Enrica Calvani, Harshil Patel, Guy Bushkin, Markus Ralser, Ambrosius P. Snijders, Mark Skehel, Ana Casanal, Schraga Schwartz, Folkert J. van Werven
Summary: N6-methyladenosine (m6A) is the most abundant mRNA modification deposited by m6A methyltransferase complexes (MTC) in mammals/insects/plants. The yeast MTC is critical for meiosis and shares similarities with mammalian MTC. The mRNA interacting module of the yeast MTC plays an important role in m6A-independent function, while other components have separate functions.
Article
Multidisciplinary Sciences
Young-Tae Lee, Alex Ayoub, Sang-Ho Park, Liang Sha, Jing Xu, Fengbiao Mao, Wei Zheng, Yang Zhang, Uhn-Soo Cho, Yali Dou
Summary: This study demonstrates how the intrinsically disordered regions of ASH2L and DPY30 restrict the rotational dynamics of MLL1 on the nucleosome core particle, leading to more efficient enzyme-substrate engagement and higher H3K4 trimethylation activity.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Franziska Greulich, Michael Wierer, Aikaterini Mechtidou, Omar Gonzalez-Garcia, N. Henriette Uhlenhaut
Summary: The SETD1A/COMPASS histone methyltransferase complex is highly enriched in activated mouse macrophages and cooperates with the glucocorticoid receptor (GR) to mediate anti-inflammatory effects. Their interaction leads to transcriptional changes in subsets of macrophage target genes.
Article
Biochemistry & Molecular Biology
Aleksandra Chikunova, Marcellus Ubbink
Summary: Evolution minimizes the number of highly conserved amino acid residues in proteins to ensure evolutionary robustness and adaptability. This study analyzes the roles of all highly conserved, non-catalytic residues in class A beta-lactamase and reveals their importance in fine-tuning the active site structure, maintaining protein folding and stability.
Article
Genetics & Heredity
Linda M. Reis, Huban Atilla, Peter Kannu, Adele Schneider, Samuel Thompson, Tanya Bardakjian, Elena V. Semina
Summary: Histone lysine methyltransferase and demethylase enzymes are crucial for chromatin organization and gene expression. This study links these genes to developmental ocular phenotypes, suggesting that individuals with eye defects should be examined for variants in these genes, especially if accompanied by other abnormalities. Nine damaging genetic variants in KMT2D and other histone lysine methyltransferases/demethylases were identified in families affected by developmental eye diseases. The study expands our understanding of the phenotypic spectrum associated with KMT and KDM factors and highlights the importance of genetic testing for accurate diagnosis.
Review
Cardiac & Cardiovascular Systems
Jun-yi Zhu, Joyce van de Leemput, Zhe Han
Summary: Epigenetic marks, such as lysine-specific histone methylation, play crucial roles in development by regulating the structure of the genome. Dysregulation of histone lysine methylation has been associated with heart disease, and genes involved in this process are overrepresented in congenital heart disease. This review summarizes the evidence supporting the role of specific lysine-specific methyltransferases in heart development and disease, including genetic associations and animal studies, and discusses new opportunities for treatment.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Cell Biology
Meagan Jezek, Winny Sun, Maraki Y. Negesse, Zachary M. Smith, Alexander Orosz, Erin M. Green
Summary: Set1 is an H3K4 methyltransferase involved in various genomic functions. It plays a role in subtelomeric gene repression through its catalytic activity towards H3K4 and regulates telomere length through its catalytic activity but likely independent of the H3K4 substrate. Set1 also calibrates the abundance of critical telomere maintenance proteins through transcriptional and posttranscriptional pathways. These findings provide new insights into the roles of Set1 in telomere maintenance and have implications for Set1-related methyltransferases in other systems.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Genetics & Heredity
Jiya Singh, Prashath Karunaraj, Max Luf, Cathie M. Pfleger
Summary: Ras signaling is crucial in growth, proliferation, and development, and its ubiquitination events are highly conserved between flies and mammals. By utilizing mutant forms of Ras, it has been shown that two lysine residues in Drosophila Ras play inhibitory roles in activation.
G3-GENES GENOMES GENETICS
(2023)
Article
Chemistry, Applied
Pengqi Bao, Li Chen, Yue Hu, Yan Wang, Cunliu Zhou
Summary: This study investigated the ability of L-arginine and L-lysine to mitigate the adverse effects of freezing on the structure and solubility of myofibrillar protein extract (MPE) in porcine Longissimus lumborum. The results showed that both L-arginine and L-lysine were effective in alleviating the adverse effects of freezing. These findings are important in the meat industry.
Article
Chemistry, Medicinal
Xin Chen, Junjie Xu, Xianghan Wang, Guanlu Long, Qidong You, Xiaoke Guo
Summary: WDR5, a member of the WD40 protein family, is involved in various biological activities and diseases. It is considered a potential target for disease treatment, and inhibitors targeting WDR5 have shown great development potential and application prospects.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Organic
Vincenzo Battaglia, Sara Meninno, Alessandra Lattanzi
Summary: In this study, a novel and concise method for the synthesis of the commonly used antiplatelet drug Plavix was developed. Through careful optimization of reaction conditions, a high synthesis yield and selectivity were achieved.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Jun-Min Lee, Min-Ho Park, Bu-Soo Park, Min-Kyu Oh
Summary: This study achieved microbial production of S-methyl-methionine (SMM) by introducing a plant-derived MMT gene into engineered Saccharomyces cerevisiae sake K6.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Review
Multidisciplinary Sciences
Marc A. J. Morgan, Ali Shilatifard
Summary: In the past three decades, research on chromatin has provided valuable information about the mechanisms that regulate transcription. The histone code hypothesis has guided this research and uncovered fundamental mechanisms relevant to biology. Recent studies have shown that histone-modifying enzymes have noncatalytic functions beyond their enzymatic activities. This review highlights the latest advances in understanding these noncatalytic functions and calls for further research to explore the epigenetic moonlighting functions of chromatin-modifying enzymes.
Article
Microbiology
Pei-Liang Ye, Bing Yuan, Xue-Qing Wang, Ming-Ming Zhang, Xin-Qing Zhao
Summary: This study reveals that modification of Set5 phosphorylation sites regulates the expression and phosphorylation of Hog1, expanding current knowledge on upstream regulation of the Hog1 stress signaling network.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cell & Tissue Engineering
Jianying Zhang, Daibang Nie, Kelly Williamson, Jorge L. Rocha, MaCalus Hogan, James H-C Wang
JOURNAL OF TISSUE ENGINEERING
(2019)
Article
Chemistry, Medicinal
James McNulty, Chanti Babu Dokuburra, Leonardo D'Aiuto, Matthew Demers, Lora McClain, Paolo Piazza, Kelly Williamson, Wenxiao Zheng, Vishwajit L. Nimgaonkar
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2020)
Article
Cell Biology
Jianying Zhang, Feng Li, Kelly M. Williamson, Susheng Tan, Devon Scott, Kentaro Onishi, MaCalus V. Hogan, James H. -C. Wang
Summary: This study characterized the porcine Achilles tendon (PAT) in terms of its structural components, vascularity, and resident tendon cells. The findings revealed the presence of blood vessel-like tissues in the paratenon and IFM, with cells expressing characteristics of vascular stem/progenitor cells. The cells isolated from paratenon and IFM displayed the ability for multi-differentiation and expressed various markers associated with stem/progenitor cells. These findings contribute to a better understanding of the vascular and cellular mechanisms involved in tendon homeostasis, injury, healing, and regeneration.
CELL AND TISSUE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Jianying Zhang, Daibang Nie, Kelly Williamson, Arthur McDowell, MaCalus V. Hogan, James H-C. Wang
Article
Multidisciplinary Sciences
Jianying Zhang, Feng Li, Tyler Augi, Kelly M. Williamson, Kentaro Onishi, MaCalus Hogan, Matthew D. Neal, James H-C Wang
Summary: Platelet-rich plasma (PRP) containing PLT HMGB1 plays a crucial role in tendon wound healing by reducing inflammation, increasing local HMGB1 levels, and recruiting stem cells to the injured area. The efficacy of PRP treatment for tendon injuries in clinics may be dependent on the presence of PLT HMGB1 in PRP preparations.
Article
Critical Care Medicine
Nijmeh Alsaadi, Adnan Hassoune, Shannon Haldeman, Kelly M. Williamson, William Plautz, Lara Hoteit, Jurgis Alvikas, Elizabeth A. Andraska, Amudan J. Srinivasan, Jillian Bonaroti, Anupamaa Seshadri, Roberto Mota-Alvidrez, Melanie J. Scott, Paul A. Gardner, Carl H. Snyderman, Matthew D. Neal
Summary: The study found that lactated Ringer's (LR) was superior to normal saline (NS) and PlasmaLyte in achieving hemostasis during intraoperative irrigation. Warming the irrigation fluid significantly reduced bleeding time. Adding Ca2+ and tranexamic acid (TXA) to NS also reduced bleeding time and blood loss, but LR remained more effective.
Article
Chemistry, Medicinal
Carla E. Brown, Tiffany Kong, James McNulty, Leonardo D'Aiuto, Kelly Williamson, Lora McClain, Paolo Piazza, Vishwajit L. Nimgaonkar
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2017)
Article
Pharmacology & Pharmacy
Leonardo D'Aiuto, Kelly Williamson, Peter Dimitrion, James McNulty, Carla E. Brown, Chanti Babu Dokuburra, Alexander J. Nielsen, Wen Jing Lin, Paolo Piazza, Mark E. Schurdak, Joel Wood, Robert H. Yolken, Paul R. Kinchington, David C. Bloom, Vishwajit L. Nimgaonkar
ANTIVIRAL RESEARCH
(2017)
Article
Genetics & Heredity
LK Lewis, K Lobachev, JW Westmoreland, G Karthikeyan, KM Williamson, JJ Jordan, MA Resnick