Article
Biochemistry & Molecular Biology
Matthew Day, Antony W. Oliver, Laurence H. Pearl
Summary: The RAD9-RAD1-HUS1 (9-1-1) clamp is part of the DNA damage checkpoint system that can detect large regions of single-stranded DNA caused by replication fork collapse or DNA double-strand breaks. A study using cryogenic electron microscopy reveals the structure of the human RAD17-RFC clamp loader complex bound to human 9-1-1 at a dsDNA-ssDNA junction, providing insight into how RAD17 confers specificity for 9-1-1 and how the clamp loader recognizes the recessed 5' DNA end and orients 9-1-1 on the ssDNA.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Dongfang Li, Peter Vogel, Xiujie Li-Harms, Bo Wang, Mondira Kundu
Summary: Defects in macroautophagy/autophagy are implicated in the pathogenesis of neuromuscular and heart diseases. The study generated muscle-specific rb1cc1- and atg14-conditional knockout (cKO) mice and found that atg14-cKO mice developed hypertrophic cardiomyopathy, while rb1cc1-cKO mice showed features of autophagic vacuolar myopathy. Tissue-specific differences between skeletal and cardiac muscles in their reliance on core autophagy proteins and unique roles for ULK1-ULK2 and RB1CC1 were highlighted in the development of TARDBP(+) pathology.
Article
Neurosciences
Songxue Su, Yevgen Yudin, Nawoo Kim, Yuan-Xiang Tao, Tibor Rohacs
Summary: The study showed that TRPM3 plays a role in heat hypersensitivity and spontaneous pain after nerve injury, and TRPM3 inhibitors can effectively alleviate these symptoms.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Plant Sciences
Emilija Dukic, Kim A. van Maldegem, Kashif Mohd Shaikh, Kento Fukuda, Mats Toepel, Katalin Solymosi, Jonna Hellsten, Thomas Hesselhoj Hansen, Soren Husted, John Higgins, Satoshi Sano, Sumio Ishijima, Cornelia Spetea
Summary: Magnesium (Mg2+) is crucial for photosynthesis in plants and algae, and its concentration in chloroplasts is tightly regulated by transport proteins. This study identifies three different transporters and investigates their roles in maintaining chloroplast Mg2+ homeostasis and regulating photosynthesis in Arabidopsis thaliana and Chlamydomonas reinhardtii. The findings reveal essential but differential roles of these transporters in chloroplast Mg2+ homeostasis and highlight their conservation across plant species.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cardiac & Cardiovascular Systems
Michael Warkala, Dongying Chen, AnnJosette Ramirez, Ali Jubran, Michael Schonning, Xia Wang, Huaning Zhao, Sophie Astrof
Summary: The development of the fourth pharyngeal arch arteries involves a multistep process with contributions from SHF-derived ECs, remodeling of endothelial plexus into PAAs, and remodeling of PAAs into the aortic arch artery and its major branches. Cell-ECM interactions regulated by integrin alpha 5 beta 1 and Fn1 are essential at each developmental stage.
CIRCULATION RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Chaekyun Kim
Summary: Bone homeostasis is regulated by the balanced actions of osteoblasts and osteoclasts, which differentiate from different progenitor cells. Mitogen-activated protein kinases (MAPKs), including ERKs, play a crucial role in the differentiation and function of osteoclasts. However, the role of ERKs in osteoclast differentiation is still controversial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lirong Hu, Abdul Sammad, Congcong Zhang, Luiz F. Brito, Qing Xu, Yachun Wang
Summary: This study evaluated the changes in milk yield, physiological indicators, blood biochemical parameters, and alternative splicing patterns in lactating Holstein cows under heat stress conditions. The results showed that heat stress negatively impacted various biological processes of Holstein cows. Additionally, heat stress increased the occurrence of alternative splicing events, with genes involved in mRNA splicing, immune, and metabolic processes being enriched. Two candidate genes associated with heat stress responses in dairy cows were identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Hussan Bano, Habib-ur-Rehman Athar, Zafar Ullah Zafar, Chukwuma C. Ogbaga, Muhammad Ashraf
Summary: This study investigated the mechanism behind drought tolerance in mung bean cultivars by selecting drought-tolerant and drought-sensitive varieties and evaluating physiological parameters such as plant water status, PSII activity, proline accumulation, and antioxidants. The results indicated that drought tolerance in mung beans is associated with these physiological traits, providing valuable information for selecting drought-tolerant cultivars.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemistry & Molecular Biology
Maria J. Asins, Maria R. Romero-Aranda, Jesus Espinosa, Paloma Gonzalez-Fernandez, Emilio Jaime-Fernandez, Jose A. Traverso, Emilio A. Carbonell, Andres Belver
Summary: This study used RNAi technology to reduce the expression level of Na+ transporters in tomato. By studying different rootstock/scion combinations, it was found that silencing the SgHKT1;1 gene in the rootstock can improve salt tolerance and fruit quality of tomato varieties carrying the wild type SgHKT1;2 allele.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Marine & Freshwater Biology
Bo Zhang, Geng Qin, Lili Qu, Yanhong Zhang, Chunyan Li, Chunlei Cang, Qiang Lin
Summary: The study found that essential genes from the Wnt gene family, particularly Wnt5a, Wnt8a, and Wnt11, may play important roles in seahorse tail development. Wnt8a was observed to have a significantly extended expression period during seahorse tail development, potentially promoting caudal tail axis formation and contributing to the elongation of the seahorse's tail.
MARINE LIFE SCIENCE & TECHNOLOGY
(2021)
Article
Agronomy
Xiaosheng Zhao, Tianbo Zhang, Huijing Feng, Tiancheng Qiu, Zichao Li, Jun Yang, You-Liang Peng, Wensheng Zhao
Summary: A novel leaf senescence mutant, nbl1, was identified in rice. The causative gene OsNBL1, encoding a small conserved protein, was found to be essential for controlling leaf senescence, disease resistance, and salt tolerance by interacting with a plastidic caseinolytic protease OsClpP6.
Article
Microbiology
Hsueh-Hsia Lo, Chao-Tsai Liao, Chih-En Li, Hsiao-Ching Chang, Yi-Min Hsiao
Summary: In the phytopathogen Xanthomonas campestris pathovar campestris, the ssrA and smpB genes are shown to be involved in pathogenesis, affecting bacterial attachment, cell motility, and extracellular enzyme activity, as well as reducing temperature tolerance. This study provides the first demonstration of the roles of SsrA and SmpB in a plant pathogen.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Jonathan O. Hernandez, Byung Bae Park
Summary: This study investigated the effects of water-deficit stress on leaf anatomical traits, physiological traits, and stem starch content in two oak tree species. The results showed that both species experienced changes in leaf characteristics and physiological traits under water-deficit stress, with Quercus serrata showing higher adaptability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Qingzhuoma Yang, Yulu Ran, Shengtao Guo, Fazhi Li, Dongyou Xiang, Yu Cao, Dairong Qiao, Hui Xu, Yi Cao
Summary: In this study, two flavohemoglobin-encoding genes were characterized in a particular fungus, revealing their conservation and contrasting roles in lipid accumulation and nitric oxide detoxification. The study also identified a regulatory factor that simultaneously controls the expression of these two genes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Environmental Sciences
Yan-Fei Zhao, Qi-Qiao Wen, Chun-Mei Ao, Wei Wang, Li-Li Shi, Cheng-Gui Wang, Siuming-Francis Chan
Summary: In this study, the roles of ETH, EH, and CCAP in molting regulation in crabs were investigated. The results showed that these hormones play essential and distinct roles in the molting cycle of crabs, and there is interaction among them. These findings contribute to our understanding of the different mechanisms of molting regulation in insects and crustaceans.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Oncology
Yumiko Kinoshita, Rieko Izukura, Junji Kishimoto, Maki Kanaoka, Hayato Fujita, Koji Ando, Shuntaro Nagai, Sayuri Akiyoshi, Tetsuzo Tagawa, Makoto Kubo, Junichi Inokuchi, Kenoki Ohuchida, Eiji Oki, Kentaro Tanaka, Masatoshi Eto, Tomoharu Yoshizumi, Masafumi Nakamura, Akiko Chishaki
Summary: This study assessed the reliability, validity, and responsiveness of the Japanese version of the EORTC QLQ-ELD14 questionnaire for evaluating the health-related quality of life in elderly Japanese patients with cancer. The results showed that the questionnaire has acceptable reliability and validity in this population.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Meeting Abstract
Gastroenterology & Hepatology
R. Nakanishi, K. Morooka, K. Omori
DISEASES OF THE COLON & RECTUM
(2023)
Editorial Material
Oncology
Ryota Nakanishi, Ken'ichi Morooka, Kazuki Omori, Satoshi Toyota, Yasushi Tanaka, Hirofumi Hasuda, Naomichi Koga, Kentaro Nonaka, Qingjiang Hu, Yu Nakaji, Tomonori Nakanoko, Koji Ando, Mitsuhiko Ota, Yasue Kimura, Eiji Oki, Yoshinao Oda, Tomoharu Yoshizumi
ANNALS OF SURGICAL ONCOLOGY
(2023)
Review
Oncology
Seiya Sato, Yoshiaki Nakamura, Eiji Oki, Takayuki Yoshino
Summary: The body of evidence supporting the utility of ctDNA analysis for detecting MRD in resected CRC is rapidly growing, leading to the development of escalation and de-escalation ACT strategies. The CIRCULATE-Japan project is one of the largest randomized clinical trials investigating this approach. This review provides an overview of the potential utility of ctDNA-based MRD detection for guiding ACT in patients with resected CRC, with a focus on the findings from the CIRCULATE-Japan project.
CLINICAL COLORECTAL CANCER
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yusuke Nishimuta, Daisuke Tsurumaru, Satohiro Kai, Junki Maehara, Yoshiki Asayama, Eiji Oki, Kousei Ishigami
Summary: This study aims to assess the correlation between histopathological features of gastric cancer and the extracellular volume fraction (ECV) measured by preoperative equilibrium contrast-enhanced computed tomography (CECT). The results showed that the values of venous invasion and tumor infiltration pattern were significantly correlated with tumor ECV. Linear regression and receiver operating characteristic (ROC) curve analyses further confirmed the correlation between tumor ECV and pathological infiltration. The tumor ECV demonstrated high diagnostic accuracy for predicting infiltration.
JAPANESE JOURNAL OF RADIOLOGY
(2023)
Editorial Material
Oncology
Eiji Shinto, Eiji Oki, Mototsugu Shimokawa, Shigeki Yamaguchi, Megumi Ishiguro, Seiji Hasegawa, Yasumasa Takii, Hideyuki Ishida, Tetsuya Kusumoto, Masaru Morita, Naohiro Tomita, Manabu Shiozawa, Masafumi Tanaka, Heita Ozawa, Yojiro Hashiguchi, Shinobu Ohnuma, Sachiyo Tada, Tomoko Matsushima, Keisuke Yamagishi, Kazuo Hase
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Oncology
Eiji Shinto, Eiji Oki, Mototsugu Shimokawa, Shigeki Yamaguchi, Megumi Ishiguro, Seiji Hasegawa, Yasumasa Takii, Hideyuki Ishida, Tetsuya Kusumoto, Masaru Morita, Naohiro Tomita, Manabu Shiozawa, Masafumi Tanaka, Heita Ozawa, Yojiro Hashiguchi, Shinobu Ohnuma, Sachiyo Tada, Tomoko Matsushima, Keisuke Yamagishi, Kazuo Hase
Summary: This study confirmed the predictive power of the MBS for recurrence risk in stage II/III CC patients by using FFPE materials. The MBS-low group demonstrated better relapse-free survival compared with the MBS-high group, and the MBS score was found to be an independent prognostic factor.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Review
Oncology
Sachie Omori, Tomoko Shigechi, Kana Kawaguchi, Hideki Ijichi, Eiji Oki, Tomoharu Yoshizumi
Summary: TLS in solid tumors is a life-threatening condition that can be complicated by DIC. Early recognition and treatment of patients at risk of TLS is crucial to prevent fatal situations. This case report and literature review focuses on identifying the distinctive features and hazards of TLS in breast cancer.
ANTICANCER RESEARCH
(2023)
Article
Surgery
Eiji Oki, Mitsuhiko Ota, Tomonori Nakanoko, Yasushi Tanaka, Satoshi Toyota, Qingjiang Hu, Yu Nakaji, Ryota Nakanishi, Koji Ando, Yasue Kimura, Yuichi Hisamatsu, Koshi Mimori, Yoshiya Takahashi, Hajime Morohashi, Takahiro Kanno, Kotaro Tadano, Kenji Kawashima, Hironobu Takano, Yuma Ebihara, Masaki Shiota, Junichi Inokuchi, Masatoshi Eto, Tomoharu Yoshizumi, Kenichi Hakamada, Satoshi Hirano, Masaki Mori
Summary: This study describes the first application of a double-surgeon cockpit system to telesurgery. Non-specialized surgeons were able to safely perform cholecystectomy and renal vein ligation with remote assistance using the system, and the system also received higher technical scores compared to telesurgery performed by specialized surgeons.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)
Article
Oncology
Shin Kobayashi, Hideaki Bando, Akinobu Taketomi, Takeshi Takamoto, Eiji Shinozaki, Manabu Shiozawa, Hiroki Hara, Kentaro Yamazaki, Koji Komori, Nobuhisa Matsuhashi, Takeshi Kato, Yoshinori Kagawa, Mitsuru Yokota, Eiji Oki, Keigo Komine, Shinichiro Takahashi, Masashi Wakabayashi, Takayuki Yoshino
Summary: This study aims to evaluate the efficacy and safety of a novel triplet regimen in the perioperative period for patients with resectable BRAF V600E mutant colorectal cancer. The triplet regimen includes encorafenib, binimetinib, and cetuximab, administered for 3 cycles each before and after curative resection. The primary endpoint is the 1-year progression-free survival rate, and secondary endpoints include disease-free survival, overall survival, and objective response rate.
Article
Oncology
Koji Ando, Yoshiaki Nakamura, Hiroyuki Kitao, Mototsugu Shimokawa, Daisuke Kotani, Hideaki Bando, Tomohiro Nishina, Takanobu Yamada, Satoshi Yuki, Yukiya Narita, Hiroki Hara, Takashi Ohta, Taito Esaki, Yasuo Hamamoto, Ken Kato, Yoshiyuki Yamamoto, Keiko Minashi, Koushiro Ohtsubo, Naoki Izawa, Hisato Kawakami, Takeshi Kato, Taroh Satoh, Naohiro Okano, Akihito Tsuji, Kentaro Yamazaki, Takayuki Yoshino, Yoshihiko Maehara, Eiji Oki
Summary: This study evaluated the impact of TP53 gene mutation on the efficacy of Nivolumab treatment in gastric cancer patients and found that TP53 wild type patients had a higher objective response rate than mutant patients, and among mutant patients, the frameshift type had the highest response rate.
BRITISH JOURNAL OF CANCER
(2023)
Article
Oncology
Shinichiro Ikeda, Qingjiang Hu, Keita Natsugoe, Tomoya Harima, Yasushi Tanaka, Izumi Kinoshita, Kentaro Nonaka, Sho Nambara, Ryota Nakanishi, Tomonori Nakanoko, Mitsuhiko Ota, Yasue Kimura, Eiji Oki, Yoshinao Oda, Tomoharu Yoshizumi
Summary: This case report describes a rare case of primary adenocarcinoma of the duodenum with high microsatellite instability (MSI-H). The patient was diagnosed with Lynch syndrome, which is closely related to MSI-H. Pembrolizumab therapy was effective in treating the tumor.
INTERNATIONAL CANCER CONFERENCE JOURNAL
(2023)
Proceedings Paper
Computer Science, Hardware & Architecture
Akio Kawabata, Bijoy Chand Chatterjee, Eiji Oki
Summary: This paper proposes a multi-homing network design model named MHND, which balances low delay and high availability for distributed processing applications. MHND maintains event occurrence order using conservative synchronization and formulates an integer linear programming problem to minimize delay. Numerical results show that increasing the number of multi-homing servers improves availability but also increases delay. Two or more multi-homing can achieve approximately 10 times higher availability compared to single-homing at the cost of a 1.25 times delay increase. MHND enables flexible network design based on required availability and acceptable delay.
2023 IEEE 20TH CONSUMER COMMUNICATIONS & NETWORKING CONFERENCE, CCNC
(2023)
Article
Biochemistry & Molecular Biology
Eiji Oki, Takayuki Yoshino
Summary: The ongoing CIRCULATE-Japan trial is investigating the role of ctDNA-based molecular residual disease testing in patients with resectable colorectal cancer after surgery. An interim analysis of GALAXY, a prospective observational arm, confirms ctDNA as a prognostic and predictive biomarker.
Article
Surgery
Yuma Ebihara, Satoshi Hirano, Hironobu Takano, Takahiro Kanno, Kenji Kawashima, Hajime Morohashi, Eiji Oki, Kenichi Hakamada, Shigeo Urushidani, Masaki Mori
Summary: This study evaluated the performance of robotic tele-cholecystectomy using the Saroa(TM) system. The results showed that there was no significant difference in completion time, technical assessment, and fatigue between tele-cholecystectomy and local surgery, but the total path length of the forceps was longer in tele-cholecystectomy.
JOURNAL OF ROBOTIC SURGERY
(2023)