Article
Multidisciplinary Sciences
Andrew T. Meek, Nils M. Kronenberg, Andrew Morton, Philipp Liehm, Jan Murawski, Eleni Dalaka, Jonathan H. Booth, Simon J. Powis, Malte C. Gather
Summary: The authors introduce an interference-based method illuminated by two rapidly alternating wavelengths for real-time mapping of nanoscale forces with sub-second mechanical fluctuations. This technique allows monitoring of cellular forces with high throughput and excellent temporal resolution, providing a new approach for studying dynamic processes in mechanobiology.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Kevin M. Dean, Tonmoy Chakraborty, Stephan Daetwyler, Jinlong Lin, Gerard Garrelts, Ons M'Saad, Hannahmariam T. Mekbib, Fabian F. Voigt, Martina Schaettin, Esther T. Stoeckli, Fritjof Helmchen, Joerg Bewersdorf, Reto Fiolka
Summary: The protocol provides detailed instructions for assembling and operating a versatile variant of light-sheet fluorescence microscopy called axially swept light-sheet microscopy (ASLM), which offers an unparalleled combination of field of view, optical resolution, and optical sectioning. It includes information on the working principle, applications, practical tips, part lists, schematics, and software for instrument control of ASLM.
Article
Acoustics
Takeshi Hirasawa, Kazuyoshi Tachi, Manami Miyashita, Shinpei Okawa, Toshihiro Kushibiki, Miya Ishihara
Summary: This study proposes a new spectroscopic OR-PAM technique to acquire information on the photoacoustic signal intensity and excitation wavelength from single spatial scans, which is validated by measuring the excitation wavelengths of dyes in tubes and demonstrating the three-dimensional spectroscopic OR-PAM of cells stained with suitable dyes.
Article
Optics
Liao Ma, Ning Leng, Ming Jin, Ming Bai
Summary: This paper presents an innovative optically controlled plasma scattering technique for imaging electromagnetic fields. The plasma induced by the photoconductive effect is used as an optically controlled scattering probe to image the amplitude and phase of electromagnetic fields in real time. This method offers advantages such as ultrafast speed, super-resolution, ultra-wideband response, low-cost, and vectorial wave mapping ability.
Article
Multidisciplinary Sciences
Yao L. Wang, Noa W. F. Grooms, Sabrina C. Civale, Samuel H. Chung
Summary: Confocal microscopes can reject out-of-focus and scattered light, but widefield microscopes are more common due to accessibility and cost. This study demonstrates confocal imaging capacity on a widefield microscope using a spatial light modulator (SLM) and customized methods. SLM enhancement improves optical sectioning capability, reducing background and enabling visualization of dim neuronal fibers.
Article
Chemistry, Analytical
Nina Ogrinc, Alexandre Kruszewski, Paul Chaillou, Philippe Saudemont, Chann Lagadec, Michel Salzet, Christian Duriez, Isabelle Fournier
Summary: The study developed a novel method for in vivo mass spectrometry imaging, using SpiderMass technology and a high-accuracy robotic arm. By moving the laser probe above the sample surface and equipping the robotic arm with a sensor, images can be acquired and molecular data can be plotted directly onto a 3D topographical image without the need for image fusion.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Bo-Jui Chang, James D. Manton, Etai Sapoznik, Theresa Pohlkamp, Tamara S. Terrones, Erik S. Welf, Vasanth S. Murali, Philippe Roudot, Kayley Hake, Lachlan Whitehead, Andrew G. York, Kevin M. Dean, Reto Fiolka
Summary: Multiangle projection imaging accelerates volumetric imaging by converting any camera-based microscope with optical sectioning capability into a system that can capture images from multiple perspectives. This method reduces data overhead, speeds up imaging, and allows for real-time stereoscopic imaging and three-dimensional particle localization. The technology has been successfully demonstrated on various microscopes and specimens, showing potential applications in cancer cell morphodynamics, calcium signaling, and simultaneous imaging of multiple perspectives of a beating heart.
Article
Optics
Jingdan Liu, Charlotte Zaouter, Xianglei Liu, Shunmoogum A. Patten, Jinyang Liang
Summary: Researchers have developed a new dispersion-eliminated CALF imaging technology that can record light field information with high speed and resolution, and applied it to multi-perspective viewing, digital refocusing, and 3D tracking of dynamic and static scenes. Experimental results demonstrate the potential application value of this technology in imaging and analyzing zebrafish behaviors.
Article
Ophthalmology
Po-Yi Lee, Bin Yang, Yi Hua, Susannah Waxman, Ziyi Zhu, Fengting Ji, Ian A. Sigal
Summary: This study evaluated the imaging capability of instant polarized light microscopy (IPOL) for important aspects of the architecture and dynamics of the optic nerve head (ONH). The results showed that IPOL can capture details of collagen fiber orientation, density, and morphology with high temporal and spatial resolutions. The study also revealed the complex biomechanics of the ONH, including the deformation of collagen fibers in the peripapillary sclera (PPS) and lamina cribrosa (LC) under load, as well as the heterogeneous deformation of LC pores.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Cell Biology
Jingfang K. Zhang, Michael Fanous, Nahil Sobh, Andre Kajdacsy-Balla, Gabriel Popescu
Summary: The current surgical pathology workflow faces challenges in terms of subjectivity and standardization. In this study, we applied a label-free method called SLIM and a deep learning algorithm to achieve automated colorectal cancer screening with high accuracy. This approach could potentially be a valuable clinical tool, enabling faster and more accurate assessments by pathologists.
Article
Optics
Cheng Zhou, Xuan Liu, Yueshu Feng, Xinwei Li, Gangcheng Wang, Haizhu Sun, Heyan Huang, Lijun Song
Summary: A new real-time computational ghost imaging method based on deep learning technology and array spatial light field modulation is proposed, which can achieve high-quality imaging and physical compression imaging of objects.
OPTICS AND LASERS IN ENGINEERING
(2022)
Article
Optics
Chengxin Zhou, Tao Huang, Zhenghui Wang, Shengde Liu, Liyun Zhong, Giancarlo Pendrini, Caojin Yuan, Xiaoxu Lu
Summary: The study introduces a quantitative differential interference contrast microscopy system that utilizes a liquid crystal shearing assembly, allowing for DIC imaging with arbitrary shearing amount and direction. By compensating for errors using a quarter-wave plate and a mirror, the system demonstrates high practical value in quantitative phase measurement.
OPTICS AND LASERS IN ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Mei Wang, Chiwoo Park, Taylor J. Woehl
Summary: This study utilized liquid phase transmission electron microscopy to directly observe the self-assembly of platinum nanoparticles into dense 3D supraparticles over tens of seconds during colloidal nanoparticle synthesis. Varying interparticle interactions by changing precursor chemistry revealed that supraparticle formation was driven by weak attractive van der Waals forces balanced by short ranged repulsive steric interactions. Growth kinetic measurements and an interparticle interaction model showed that nanoparticle surface diffusion rates on the supraparticles were significantly faster than nanoparticle attachment.
Article
Multidisciplinary Sciences
Azeem Ahmad, Vishesh Dubey, Nikhil Jayakumar, Anowarul Habib, Ankit Butola, Mona Nystad, Ganesh Acharya, Purusotam Basnet, Dalip Singh Mehta, Balpreet Singh Ahluwalia
Summary: High space-bandwidth product and spatial phase sensitivity are crucial for single-shot quantitative phase microscopy (QPM) systems. The use of pseudo-thermal light source (PTLS) overcomes the limitations of traditional light sources in QPM, enabling better performance. PTLS achieves spatial phase sensitivity equivalent to white light sources and supports a field of view equivalent to laser sources, making it suitable for wider applications in life sciences and clinical settings.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Stephan Allgeier, Andreas Bartschat, Sebastian Bohn, Rudolf F. Guthoff, Veit Hagenmeyer, Lukas Kornelius, Ralf Mikut, Klaus-Martin Reichert, Karsten Sperlich, Nadine Stache, Oliver Stachs, Bernd Koehler
Summary: This article describes a novel approach for real-time creation and visualization of a mosaic image of the SNP, allowing for evaluation of the acquired image data quality during the examination. Online mosaicking also offers the chance to identify overlap with previous images, which is particularly advantageous for follow-up examinations.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Ritu Raman, Basanta Bhaduri, Mustafa Mir, Artem Shkumatov, Min Kyung Lee, Gabriel Popescu, Hyunjoon Kong, Rashid Bashir
ADVANCED HEALTHCARE MATERIALS
(2016)
Article
Biochemical Research Methods
Basanta Bhaduri, Ryan M. Nolan, Ryan L. Shelton, Lara A. Pilutti, Robert W. Motl, Stephen A. Boppart
JOURNAL OF BIOMEDICAL OPTICS
(2016)
Article
Optics
Ravi Kumar, Basanta Bhaduri, Bryan Hennelly
JOURNAL OF MODERN OPTICS
(2018)
Article
Optics
Ravi Kumar, Basanta Bhaduri
OPTICS AND LASER TECHNOLOGY
(2017)
Article
Optics
Ravi Kumar, Basanta Bhaduri, Naveen K. Nishchal
Article
Optics
Ravi Kumar, Basanta Bhaduri
Article
Optics
Ravi Kumar, John T. Sheridan, Basanta Bhaduri
OPTICS AND LASER TECHNOLOGY
(2018)
Article
Optics
Basanta Bhaduri, Murat Yessenov, Ayman F. Abouraddy
Article
Optics
Basanta Bhaduri, Murat Yessenov, Ayman F. Abouraddy
Article
Optics
Murat Yessenov, Lam Mach, Basanta Bhaduri, Davood Mardani, H. Esat Kondakci, George K. Atia, Miguel A. Alonso, Ayman F. Abouraddy
Article
Optics
Basanta Bhaduri, Murat Yessenov, Danielle Reyes, Jessica Pena, Monjurul Meem, Shermineh Rostami Fairchild, Rajesh Menon, Martin Richardson, Ayman F. Abouraddy
Article
Optics
Murat Yessenov, Basanta Bhaduri, H. Esat Kondakci, Monjurul Meem, Rajesh Menon, Ayman F. Abouraddy
Article
Optics
Murat Yessenov, Alyssa M. Allende Motz, Basanta Bhaduri, Ayman F. Abouraddy
Summary: The refraction of space-time wave packets at planar interfaces between non-dispersive, homogeneous, isotropic dielectrics exhibits fascinating phenomena, even at normal incidence. Crucial differences emerge at oblique incidence with respect to the results established at normal incidence, such as the ability to tune the group velocity simply by changing the angle of incidence. An experimental verification of the refractive phenomena exhibited by ST wave packets at oblique incidence is provided, along with a proposal for blind synchronization, which has been demonstrated experimentally for the first time.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2021)
Article
Optics
Murat Yessenov, Alyssa M. Allende Motz, Basanta Bhaduri, Ayman F. Abouraddy
Summary: This paper theoretically described the refraction of ST wave packets at a planar interface in the first paper of the series, and experimentally verified the refraction of baseband ST wave packets at normal incidence in the second paper. The observed phenomena include group velocity invariance, normal and anomalous refraction, and group velocity inversion leading to group delay cancellation.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2021)
Article
Optics
Murat Yessenov, Basanta Bhaduri, Ayman F. Abouraddy
Summary: In this study, the refraction of ST wave packets at planar interfaces between non-dispersive, homogeneous, and isotropic dielectrics is examined. A new law of refraction is proposed, highlighting fascinating phenomena such as group-velocity invariance, anomalous refraction, group-velocity inversion, and the dependence of group velocity on the angle of incidence.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2021)