Article
Biochemistry & Molecular Biology
Andrea Monteon, Lorelei Hughes, Victor Camberos, Mary Kearns-Jonker
Summary: By studying cardiac progenitor cells (CPCs) in human neonates, it was found that the Isl-1+ cells exhibit enhanced stemness properties, express SALL4, and have the potential for differentiation. These findings have potential value for the development of novel regenerative therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Jie Ren, Danxiu Miao, Yanshu Li, Rui Gao
Summary: Cardiac transcription factors, such as Isl1, play a crucial role in controlling cardiovascular development. Through extensive studies, Isl1 has been identified as a key player in cardiovascular development, especially as a marker gene for the second heart field progenitors. Understanding the roles of Isl1 provides insights into the molecular basis of cardiovascular diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Ziyi Song, Alus M. Xiaoli, Youlei Li, Gerile Siqin, Tian Wu, Randy Strich, Jeffrey E. Pessin, Fajun Yang
Summary: CCNC-Mediator plays an important role in the development of brown adipose tissue (BAT) and lipid accumulation in brown adipocytes. Conditional knock-out of CCNC gene leads to BAT paucity and affects brown adipose tissue development and cell proliferation, but not cell death. CCNC in brown adipocytes regulates the expression of lipogenic genes through the C/EBP alpha/GLUT4/ChREBP pathway.
MOLECULAR METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Ali Alaqla, Yang Hu, Shengyuan Huang, Sunniva Ruiz, Toshihisa Kawai, Xiaozhe Han
Summary: Immune regulatory B cells activated by Toll-like receptors (TLRs) play a crucial role in reducing inflammation and bone loss associated with periodontitis. This study investigates the role of TLR9 signaling in the activation and IL-10 production of primed B cells. The results demonstrate that TLR9 signaling is essential for the induction of IL-10 production in B cells after stimulation with P. gingivalis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Eva Persyn, Sigrid Wahlen, Laura Kiekens, Wouter Van Loocke, Hannah Siwe, Els Van Ammel, Zenzi De Vos, Filip Van Nieuwerburgh, Patrick Matthys, Tom Taghon, Bart Vandekerckhove, Pieter Van Vlierberghe, Georges Leclercq
Summary: IRF2 plays an important role in the differentiation and functional maturation of human NK cells. Its expression levels directly affect cell numbers and functions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Ru Zhang, Yi-Xuan Tu, Ding Ye, Zhenglong Gu, Zhen-Xia Chen, Yonghua Sun
Summary: This study identifies zebrafish pld6 as a novel germline-specific gene that plays a crucial role in the maintenance and differentiation of germline stem and progenitor cells (GSPCs). Pld6 knockout mutants develop exclusively into infertile males, as the GSPCs fail to differentiate and undergo apoptosis. Furthermore, pld6-depleted GSPCs display impaired mitochondrial fusion, defects in piRNA biogenesis, and transposon suppression.
Article
Multidisciplinary Sciences
Al Shaimaa Hasan, Lan Luo, Satoko Baba, Tao-Sheng Li
Summary: This study found that estrogen can increase the yield of cardiac stem cells and reverse the quality impairment caused by estrogen deficiency. The research also showed that estrogen can enhance the proliferative activity of stem cells, improve telomerase activity, and reduce DNA damage.
Article
Endocrinology & Metabolism
Francesca Tonelli, Laura Leoni, Valentina Daponte, Roberta Gioia, Silvia Cotti, Imke A. K. Fiedler, Daria Larianova, Andy Willaert, Paul J. Coucke, Simona Villani, Bjoern Busse, Roberta Besio, Antonio Rossi, P. Eckhard Witten, Antonella Forlino
Summary: This study used zebrafish to investigate the role of TRIC-B in skeletal tissue. The researchers found that the tmem38b gene is expressed at early developmental stages in zebrafish, while tmem38a gene is not. Mutations in tmem38b resulted in under-modified collagen type I and intracellular retention. These findings contribute to our understanding of the importance of TRIC-B in bone cell differentiation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Paige V. Blinkiewicz, Makayla R. Long, Zachary A. Stoner, Elizabeth M. Ketchum, Sydney N. Sheltz-Kempf, Jeremy S. Duncan
Summary: This study demonstrates that Gata3 plays a crucial role in the development of sensory and neuronal cells in late proneurosensory stages. It is not only required for proper formation at the later proneurosensory stage, but also shows a time-sensitive and dose-dependent effect.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Yu Gao, Ruining Liu, Chenfei He, Juan Basile, Mattias Vesterlund, Marie Wahren-Herlenius, Alexander Espinoza, Cassandra Hokka-Zakrisson, Fahad Zadjali, Akihiko Yoshimura, Mikael Karlsson, Berit Carow, Martin E. Rottenberg
Summary: SOCS3 is a critical regulator of immune responses and inflammation, with its expression in thymic stromal cells being essential for T cell development and maintenance of thymus architecture. The inhibition of SOCS3 led to impaired thymocyte differentiation, proliferation, and increased apoptosis at different cell stages, ultimately affecting the generation of recent thymic emigrants in peripheral organs. The interaction between SOCS3 in thymic epithelial cells and TRIM 21 had significant implications for thymic cellularity and gene expression related to T cell selection and lympho-stromal interactions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sisi Wang, Xue Li, Qingwen Ma, Qi Wang, Junping Wu, Hongzhi Yu, Kuan Li, Yu Li, Jianhai Wang, Qiuyang Zhang, Youwei Wang, Qi Wu, Huaiyong Chen
Summary: This study reveals the importance of glutamine metabolism in alveolar epithelial regeneration during lung injury. Abnormal glutamine metabolism was observed in patients with idiopathic pulmonary fibrosis and mice with bleomycin-induced fibrosis. Inhibition of key enzymes GLS1 and GPT2 leads to reduced proliferation and differentiation of AT2 cells.
Review
Neurosciences
Nana Yang, Shiyu Sun, Guangqing Duan, Kaixuan Lv, Chen Liang, Linlin Zhang, Jielun Yu, Yaohui Tang, Guohua Lu
Summary: Depression is a major psychological disease that increases the risk of cardiovascular disease, especially acute coronary syndrome. The decrease in the number of endothelial progenitor cells is closely related to depression development. EPCs might serve as a new target for evaluating the severity of depression and its complications.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Siling Fu, Yanxiao Wang, Ennan Bin, Huanwei Huang, Fengchao Wang, Nan Tang
Summary: We used single-cell RNA sequencing to analyze the molecular features of lymphatic endothelial cells (LECs) in embryonic and postnatal day (P) 0 lungs. We found that c-JUN expression is transiently upregulated in P0 LECs. Conditional knockout of Jun in LECs impairs the opening of lung lymphatic vessels at birth, leading to fluid retention in the lungs and neonatal death. Mechanical pressure induces the expression of c-JUN in LECs, and c-JUN regulates the opening of lymphatic vessels by modulating the remodeling of the actin cytoskeleton in LECs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Ching-Fang Chang, Kari M. Brown, Yanfen Yang, Samantha A. Brugmann
Summary: Primary cilia, microtubule-based cellular organelles, play a critical role in cellular function and their dysfunction is associated with various disorders. The C2cd3 protein is essential for ciliogenesis and mutations in this gene are linked to certain ciliopathies. Genetic background was found to influence the phenotypic variation in mice models, suggesting a complex interplay between genetic factors and disease manifestation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Yorleny Vicioso, Derek P. Wong, Nand K. Roy, Nayanika Das, Keman Zhang, Parameswaran Ramakrishnan, Reshmi Parameswaran
Summary: Germline deficiency of NF-kappa B c-Rel leads to a marked decrease in cytotoxic function of NK cells, which is regulated by c-Rel through controlling the expression of perforin and granzyme B. The study reveals a crucial role of c-Rel in the cytotoxic function of NK cells through transcriptional regulation of perforin and granzyme B expressions.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Kuan-Chi Lai, Julie A. Brothers, Jason Z. Stoller
CARDIOLOGY IN THE YOUNG
(2019)
Article
Cardiac & Cardiovascular Systems
Jessica S. Gaulton, Laura M. Mercer-Rosa, Andrew C. Glatz, Erik A. Jensen, Valerie Capone, Courtney Scott, Scott M. Appel, Jason Z. Stoller, Maria V. Fraga
ECHOCARDIOGRAPHY-A JOURNAL OF CARDIOVASCULAR ULTRASOUND AND ALLIED TECHNIQUES
(2019)
Article
Pediatrics
Kerri Z. Machut, Ankur Datta, Jason Z. Stoller, Rakesh Rao, Amit Mathur, Theresa R. Grover, Zeenia Billimoria, Karna Murthy
JOURNAL OF PEDIATRICS
(2019)
Article
Biochemistry & Molecular Biology
Paul Dent, Laurence Booth, Jane L. Roberts, Junchen Liu, Andrew Poklepovic, Alshad S. Lalani, David Tuveson, Jennifer Martinez, John F. Hancock
Review
Pediatrics
Maria Fraga, Jason Z. Stoller, Christie L. Glau, Daniele De Luca, Rachel G. Rempell, Jesse L. Wenger, Chor Yek Kee, Wallis T. Muhly, Karen Boretsky, Thomas W. Conlon
Review
Pediatrics
Laura E. Miller, Jason Z. Stoller, Maria V. Fraga
CURRENT OPINION IN PEDIATRICS
(2020)
Article
Biochemistry & Molecular Biology
Christophe Glorieux, Xiaojun Xia, Yong-Qiao He, Yumin Hu, Kelly Cremer, Annie Robert, Junchen Liu, Fen Wang, Jianhua Ling, Paul J. Chiao, Peng Huang
Summary: K-ras mutations enhance PD-L1 expression through a redox-mediated mechanism, while inhibition of the FGFR1 pathway reduces PD-L1 expression and enhances T cell-mediated tumor suppression.
Article
Obstetrics & Gynecology
Thomas W. Pawlowski, Jason Z. Stoller, Natalie E. Rintoul, Holly L. Hedrick, Michael D. Quartermain, Maria Fraga
Summary: The study suggests that POCUS is more accurate in evaluating ECMO venous cannula position than plain radiography, and there is substantial agreement between POCUS and echocardiography, indicating that POCUS may be a better method for assessing ECMO venous cannula position.
JOURNAL OF PERINATOLOGY
(2021)
Article
Obstetrics & Gynecology
Jason Z. Stoller, Maria Victoria Fraga
Summary: The study found high success rates of real-time ultrasound-guided LP when performed by neonatologist, with a first attempt success rate of 65% and overall success rate of 100%. There was a 69% rate of nontraumatic LP, and CSF RBC count was inversely correlated with PMA and weight at the time of LP.
JOURNAL OF PERINATOLOGY
(2021)
Article
Multidisciplinary Sciences
Walaa E. Kattan, Junchen Liu, Dina Montufar-Solis, Hong Liang, Bhargavi Brahmendra Barathi, Ransome van der Hoeven, Yong Zhou, John F. Hancock
Summary: The cellular ER-to-PM PtdSer transport mechanism is crucial for KRAS PM localization and oncogenesis, and can be targeted for therapeutic intervention. Lipid transport proteins ORP5 and ORP8 play key roles in maintaining PtdSer levels and are essential for KRAS oncogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Critical Care Medicine
Benjamin W. Kozyak, Maria Fraga, Courtney E. Juliano, Shazia Bhombal, David A. Munson, Erik Brandsma, Jason Z. Stoller, Ankit Jain, Russell Kesman, Malorie Meshkati, Caroline Y. Noh, Aaron G. Dewitt, Andrew T. Costarino, David A. Hehir, Alan M. Groves
Summary: Real-time ultrasound guidance combined with liver pressure improves the success rate of umbilical venous cannulation, especially in neonates with congenital heart disease.
PEDIATRIC CRITICAL CARE MEDICINE
(2022)
Article
Peripheral Vascular Disease
William Corder, Jason Z. Stoller, Maria Fraga
Summary: A retrospective review of 477 ultrasound guided peripheral arterial cannulations in infants found a first attempt success rate of 65% and an overall success rate of 86%. Success rates varied significantly by arterial location, with the radial artery having the highest success rates (72%, 91%) and the posterior tibial artery having the lowest (44%, 71%). Greater age and weight were associated with higher success rates (p = 0.006, p = 0.002). The use of real-time ultrasound guidance can improve success rates and an infant's weight and selected artery are strong predictors of success. Procedural ultrasound can reduce unnecessary attempts and minimize procedure-related harm.
JOURNAL OF VASCULAR ACCESS
(2023)
Article
Pediatrics
Maria V. Fraga, Kevin C. Dysart, Jason Z. Stoller, Matthew Huber, Anysia Fedec, Laura Mercer-Rosa, Haresh Kirpalani
Summary: This study aimed to determine the efficacy of inhaled nitric oxide (iNO) in reducing pulmonary artery pressure in infants with severe bronchopulmonary dysplasia (BPD) as measured by echocardiography. The results showed that iNO significantly reduced pulmonary arterial hypertension (PAH) in infants with severe BPD.
Meeting Abstract
Critical Care Medicine
Benjamin Kozyak, Maria Fraga, Courteny Juliano, Shazia Bhombal, David Munson, Erik Brandsma, Jason Stoller, Ankit Jain, Russell Kesman, Malorie Meshkati, Caroline Noh, Aaron DeWitt, Andrew Costarino, David Hehir, Alan Groves
CRITICAL CARE MEDICINE
(2022)
Meeting Abstract
Pediatrics
Benjamin W. Kozyak, Alan M. Groves, Maria V. Fraga, Courtney E. Juliano, Shazia Bhombal, David A. Munson, Erik Brandsma, Jason Z. Stoller, Ankit Jain, Andrew T. Costarino, David A. Hehir