Article
Fisheries
Bao S. Li, Xiu J. Han, Ji Y. Wang, Zhi D. Song, Yong Z. Sun, Shi X. Wang, Bing S. Huang
Summary: The study aimed to determine the optimal dietary methionine requirement for juvenile sea cucumber Apostichopus japonicus selenka. The results showed that as dietary methionine levels increased, weight gain and specific growth rate initially increased and then decreased. Meanwhile, protein and lipid contents in body wall increased initially and then dropped, while amino acids content increased.
AQUACULTURE RESEARCH
(2021)
Article
Environmental Sciences
Juanwen Yu, Yuichi Sakai, Sayaka Mino, Tomoo Sawabe
Summary: This study assessed the changes in microbial community during the larval development of sea cucumber using a laboratory rearing system. It was found that the microbial community structure was related to the developmental stage, with significant alterations detected in the late auricularia stage.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Applied
Xianyou Wang, Mingzhu Li, Yun Bai, Yaowu Du, Shaofeng Duan
Summary: Sea cucumber Apostichopus japonicus Selenka, a traditional tonic and a major aquaculture variety in China, was found to contain four new triterpene glycosides and a known holotoxin in its spawn. The structures of the new compounds were determined through extensive spectroscopic analysis and chemical tools.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Marine & Freshwater Biology
Ye Zhao, Han Wang, Haona Wang, Hui Liu, Yanying Zhang, Jianwei Zhang, Yongrui Pi, Pei Yang, Qing Wang
Summary: This study investigated the toxic response in the intestine of juvenile A. japonicus under sulfide stress. The results showed sulfide-induced intestinal inflammation and oxidative stress, as well as changes in gut bacterial composition and metabolic homeostasis. Interestingly, certain intestinal bacteria were found to be significantly correlated with metabolic changes. These findings provide new insights into the adaptation strategies of juvenile A. japonicus under sulfide stress.
AQUATIC TOXICOLOGY
(2023)
Article
Environmental Sciences
Zhonglei Ju, Guoxiang Liao, Yulu Zhang, Nan Li, Xishan Li, Yuhang Zou, Wei Yang, Deqi Xiong
Summary: This study investigated the oxidative stress response to high temperature in the respiratory tree and body wall of the sea cucumber. The results showed that high temperature caused an increase in reactive oxygen species levels and antioxidant activities in the respiratory tree, but no significant changes were observed in the body wall. Lipid peroxidation was observed in both tissues. The respiratory tree was found to be more susceptible to high temperature changes compared to the body wall. These findings provide insights into the oxidative stress response of the sea cucumber to high temperature.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Dongxue Xu, Ji Liu, Yuanxue Gu, Yanru Chen, Chunyan Zhao, Guohua Sun, Yichao Ren, Chao Li, Bin Xia
Summary: Juvenile sea cucumbers were fed different microalgae in a 130-day experiment, leading to varying growth rates and amino acid compositions. Analysis of carbon isotopes showed fluctuations in specific amino acids. The study sheds light on the metabolism routing and nutrient utilization in sea cucumbers during their juvenile stage.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Lina Sun, Chunxi Jiang, Fang Su, Wei Cui, Hongsheng Yang
Summary: In this study, a high-quality chromosome-level genome of Apostichopus japonicus was constructed using Pacbio HiFi long-reads and Hi-C sequencing data. The assembled genome had a total length of 671.60 Mb, with contig N50 size of 17.20 Mb and scaffold N50 size of 29.65 Mb. A total of 19,828 genes were annotated, and 97.2% of BUSCO genes were fully represented. This high-quality genome of A. japonicus will contribute to the development of sustainable aquaculture practices and provide insights into their genetic makeup, evolutionary history, and ecological adaptation.
Article
Environmental Sciences
Yaoping Mei, Yuan Tian, Qinfeng Gao, Shuanglin Dong, Xueqi Li, Yuling Xu
Summary: This study aimed to evaluate the effects of different stocking densities of sea cucumber on the CO2 fluxes at the water-air interface and explore the relationship between CO2 fluxes and respiratory metabolism. The results showed that sea cucumber with moderate stocking density had higher CO2 fluxes, indicating more active respiration metabolism. Transcriptome analysis revealed differentially expressed genes related to peroxisome, fatty acid degradation, and oxidative phosphorylation pathways, which may explain the differences in respiration metabolism rates at different stocking densities.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Yaoping Mei, Zhishuai Hou, Qinfeng Gao, Shuanglin Dong, Xueqi Li, Yuling Xu
Summary: This study investigated the effects of feeding ration on the growth, physiology, carbon allocation, energy budget, and CO2 fluxes of sea cucumber. The results showed that a feeding ration of 3% led to the highest specific growth rate, while a ration of 7% resulted in negative growth. Feeding ration significantly influenced carbon intake, nitrogen intake, and energy intake. The findings provide a theoretical basis for the development of efficient low-carbon aquaculture technology and sustainable industry.
AQUACULTURE REPORTS
(2023)
Article
Engineering, Marine
Yubing Lin, Haifei Liu, Zhiming Ru, Gangqin Tu, Zixin Wang
Summary: This study investigates the three-dimensional flow structure around the sea cucumber Apostichopus japonicus using the D3Q27 multiple-relaxation-time lattice Boltzmann method. The flow patterns exhibit distinctive features at different angles of attack (0 degrees, 45 degrees, 90 degrees). Increasing inflow velocity (0.1 m/s, 0.25 m/s, 0.4 m/s) does not fundamentally change the structure of flow fields but induces more small-scale vortices and larger velocity fluctuations. This study provides new insight for future studies on the interaction between water flow and A. japonicus.
Article
Environmental Sciences
Da Huo, Lina Sun, Jingchun Sun, Chenggang Lin, Shilin Liu, Libin Zhang, Hongsheng Yang
Summary: By analyzing the regulatory roles of circRNAs in sea cucumbers under thermal and hypoxic stress, this study found that circRNAs can regulate metabolic processes, signal transduction, and ion responses in Apostichopus japonicus. Different gene ontology terms were enriched in heat-related and hypoxia-related treatments, reflecting the important functions of circRNAs in responding to stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Fisheries
Xiao Li, Ying Wang, Guangbin Liu, Xiaodong Jiang, Hongyan Li, Lei Ji
Summary: The study found that supplementing the feed of Apostichopus japonicus with 25-37.5 g/kg peptides significantly improved its growth performance, body composition, and immune capacity.
AQUACULTURE NUTRITION
(2021)
Article
Zoology
Bing Zhang, Jing-Wen Yang, Tao Han, De-Xiang Huang, Zi-Hao Zhao, Jia-Qian Feng, Nai-Ming Zhou, Hong-Qing Xie, Tian-Ming Wang
Summary: In this study, a novel 5-HT receptor (Aj5-HT4/6) from the sea cucumber Apostichopus japonicus was identified and characterized. The receptor was found to be involved in the movement and metabolism of sea cucumbers. Experiments showed that treatment with 5-HT induced internalization of Aj5-HT4/6 into the cytoplasm and led to an increase in cAMP levels and MAP kinase phosphorylation in a dose-dependent manner.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ming Guo, Minjie Gui, Xi Xu, Xuemei Duan, Xuelin Zhao, Weiwei Zhang, Yina Shao, Bei Wang, Jing Diao, Chenghua Li
Summary: MiRNAs are closely related to disease occurrence, development, and immune response. BAG2, a member of the BAG family, is identified as an anti-apoptotic protein during Vibrio splendidus infection in sea cucumbers. MiR-375 inhibits the anti-apoptotic activity of AjBAG2 by targeting its 3'-UTR sequence through perfect complementarity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Da Huo, Fang Su, Hongsheng Yang, Lina Sun
Summary: This study reported exosomes secreted by sea cucumber Apostichopus japonicus, which were cup-shaped and had an average size of 74.65 nm, and identified specific exosome biomarkers (HSP70, TSG101, and CD9). The global expression of microRNAs in exosomes from normal and heat-stressed A. japonicus was analyzed, revealing increased exosome production and altered expression profiles of microRNAs under heat stress. Several differentially expressed microRNAs were commonly found, indicating their importance in the heat stress response. Target gene analysis revealed their involvement in replication and repair during the initial response and metabolism adjustments during acclimation to high temperature conditions. These findings provide insights into the regulatory roles of exosomes and microRNAs in sea cucumber's response to environmental stresses exacerbated by global warming.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Plant Sciences
Shidong Yue, Yi Zhou, Shaochun Xu, Xiaomei Zhang, Mingjie Liu, Yongliang Qiao, Ruiting Gu, Shuai Xu, Yu Zhang
Summary: The study highlighted the competitive effects of the exotic plant S. alterniflora on seagrass Z. japonica, with S. alterniflora invasion leading to inhibition of Z. japonica growth and significant increase in the distribution area of the invader. S. alterniflora primarily propagates via seeds and rhizomes, posing a threat to the population ecology of seagrass.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Fisheries
Xujia Liu, Xugang He, Guoqiang Huang, Yi Zhou, Junxiang Lai
Summary: The study shows that mullets can effectively ingest and absorb organic deposits produced by white shrimp farming, and polyculture of shrimp and mullets not only does not reduce shrimp yield, but also improves shrimp survival rate and food utilization efficiency, while enhancing water quality.
Article
Environmental Sciences
Shaochun Xu, Yongliang Qiao, Shuai Xu, Shidong Yue, Yu Zhang, Mingjie Liu, Xiaomei Zhang, Yi Zhou
Summary: Seagrass beds are highly productive ecosystems along temperate and tropical coastlines globally. This study in northern China identified four seagrass species in the Liaodong Peninsula, with seagrass bed area decreasing significantly with increasing water depth. Human activities pose a threat to these seagrass beds, highlighting the importance of conservation efforts.
MARINE POLLUTION BULLETIN
(2021)
Article
Environmental Sciences
Shaochun Xu, Zonghe Yu, Yi Zhou, Feng Wang, Shidong Yue, Xiaomei Zhang
Summary: The study conducted in a maricultural area in northern China found that the concentrations of trace elements in kelp and seawater were within safe limits, and kelp demonstrated high bioremediation capability for trace elements.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Limnology
Xiaoyue Song, Yi Zhou, Jiangning Zeng, Lu Shou, Xiaomei Zhang, Shidong Yue, Wei Gao, Weihua Feng, Zhifu Wang, Ping Du
Summary: The study found that seagrass Z. japonica developed more complex root systems in oligotrophic sediments to acquire more nutrients, while the lower root density in eutrophic sediments was mainly due to fewer shoots and shorter longevity. The trophic status of sediments has a certain influence on the phenotypic plasticity of Z. japonica root systems.
JOURNAL OF OCEANOLOGY AND LIMNOLOGY
(2021)
Article
Ecology
Shaochun Xu, Pengmei Wang, Feng Wang, Xiaomei Zhang, Xiaoyue Song, Yi Zhou
Summary: Seed germination and seedling establishment of Zostera marina L. are significantly influenced by water depth and seed burial depth, with seeds buried at shallower depths in sand showing higher percentages of successful germination and establishment. However, once plants are established, sediment type and burial depth do not play a decisive role in plant growth.
MARINE ECOLOGY PROGRESS SERIES
(2021)
Article
Environmental Sciences
Shidong Yue, Xiaomei Zhang, Shaochun Xu, Mingjie Liu, Yongliang Qiao, Yu Zhang, Junhua Liang, Andong Wang, Yi Zhou
Summary: Seagrass meadows are vital ecosystems that are under severe threat due to increasing extreme climate events. The study focused on the impact of super typhoon Lekima on Zostera japonica in the Yellow River Delta, resulting in a significant decrease in distribution area and loss of soil organic carbon and nitrogen. Despite suitable environmental conditions, recovery of the seagrass meadows is deemed impossible without restoration efforts. Long-term monitoring is essential for providing new insights and future protection of these ecosystems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Plant Sciences
Yu Zhang, Shaochun Xu, Shidong Yue, Xiaomei Zhang, Yongliang Qiao, Mingjie Liu, Yi Zhou
Summary: Based on observations and experiments on eelgrass seeds in Swan Lake and Qingdao Bay in northern China, it was found that although the seeds matured at the same time, their germination time varied. Further analysis revealed that this difference was determined by internal molecular mechanisms rather than environmental factors. Comparative transcriptome analysis showed that eelgrass seeds in Swan Lake had a deeper dormancy depth and required the mobilization of more related genes to break dormancy and initiate germination compared to seeds in Qingdao Bay.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Shaochun Xu, Shuai Xu, Yi Zhou, Shidong Yue, Xiaomei Zhang, Ruiting Gu, Yu Zhang, Yongliang Qiao, Mingjie Liu, Yunling Zhang, Zhenhai Zhang
Summary: This study conducted field sampling over 19 months in a large seagrass meadow in a eutrophic bay in northern China to investigate eelgrass population reproduction status and ecological interactions. The results showed that asexual growth was important for maintaining existing meadows, while sexual reproduction played a critical role in colonization of new areas. Nutrient uptake by seagrass might reduce the probability of a red tide in the study area.
MARINE POLLUTION BULLETIN
(2022)
Article
Ecology
Shaochun Xu, Yi Zhou, Yongliang Qiao, Shidong Yue, Xiaomei Zhang, Yu Zhang, Mingjie Liu, Yunling Zhang, Zhenhai Zhang
Summary: This study describes a novel method using seed ball burial for eelgrass restoration, and tests its effectiveness in different environmental conditions. The results show that the seed ball restoration method has varying effects at different sites, and there is an environmental mismatch in large-scale restoration.
RESTORATION ECOLOGY
(2023)
Article
Multidisciplinary Sciences
Shaochun Xu, Yu Zhang, Yi Zhou, Shuai Xu, Shidong Yue, Mingjie Liu, Xiaomei Zhang
Summary: This study investigated the distribution of eelgrass at its southern limits on the eastern coast of China and found a northward shift in the southern limit. Further experiments showed that high water temperatures negatively affected eelgrass growth and caused shoot mortality. These findings indicate that suitable eelgrass habitat has moved further north along the eastern coast of China.
Article
Environmental Sciences
Yongliang Qiao, Yu Zhang, Shaochun Xu, Shidong Yue, Xiaomei Zhang, Mingjie Liu, Lingling Sun, Xiaoping Jia, Yi Zhou
Summary: This study examined the toxic effects of copper and cadmium on eelgrass at multiple levels. The results showed that copper was more toxic to eelgrass than cadmium. High temperatures increased heavy metal damage, while low temperatures inhibited damage. Copper and cadmium had a synergistic effect on photosynthesis. Transcriptome analysis revealed severe damage to the photosynthetic system under cadmium stress, while copper stress resulted in up-regulation of genes related to glutathione metabolism. Recovery experiments showed that the damage caused by short-term copper and cadmium stress was reversible. These findings provide insights into the heavy metal toxic effects and resistance strategies of eelgrass, highlighting its potential for phytoremediation, especially for cadmium.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Shaochun Xu, Zonghe Yu, Yi Zhou, Shidong Yue, Junhua Liang, Xiaomei Zhang
Summary: Large-scale kelp aquaculture in Ailian Bay, northern China, can remove 1309 tons of carbon, 104 tons of nitrogen, and 12 tons of phosphorus, effectively combating eutrophication. The study provides evidence for the hypothesis that farmed kelp can remove nutrients and demonstrates the bioremediation potential of kelp farming.
MARINE POLLUTION BULLETIN
(2023)
Article
Environmental Sciences
Ruiting Gu, John Statton, Susi Rahmawati, Renae Hovey, Yi Zhou, Jianwu Tang, Shuo Yu, Gary A. Kendrick
Summary: The seed bank of Halophila ovalis plays a crucial role in resilience and restoration. Seasonal changes in seed abundance and viability were studied, and a viability test was conducted. The results showed differences in seed density and low proportion of viable seeds in the seed bank. Low salinity and extreme rainfall events could affect seed viability and germination, limiting population growth.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Green & Sustainable Science & Technology
Mingjie Liu, Shaochun Xu, Shidong Yue, Yongliang Qiao, Yu Zhang, Xiaomei Zhang, Yi Zhou
Summary: This study proposes a method to evaluate the seed provision efficacy of detached eelgrass reproductive shoots. Mature seeds were collected from detached eelgrass reproductive shoots in Swan Lake, China, using in situ net cages. The results showed that each detached reproductive shoot produced an average of 50 viable seeds, and the potential seed yield overestimated the actual seed yields. The simplest treatment (a single reproductive shoot) was convenient and robust for this method, indicating its potential for improving the efficiency of seed use in restoration projects.
