Article
Biochemistry & Molecular Biology
Benjamin L. Springstein, Dennis J. Nurnberg, Christian Woehle, Julia Weissenbach, Marius L. Theune, Andreas O. Helbig, Iris Maldener, Tal Dagan, Karina Stucken
Summary: Filament-forming proteins play crucial roles in cellular processes such as cell division and growth. Two novel coiled-coil-rich proteins in the filament-forming cyanobacterium Anabaena assemble into heteropolymers and function in maintaining the multicellular shape. Their function is achieved through cooperation with other proteins to stabilize the cell shape.
Article
Microbiology
Li Wang, Tian-Cai Niu, Ana Valladares, Gui-Ming Lin, Ju-Yuan Zhang, Antonia Herrero, Wen-Li Chen, Cheng-Cai Zhang
Summary: This study investigates the regulation of FtsZ during heterocyst differentiation, revealing that the interaction between PatD and FtsZ interferes with FtsZ assembly, affecting cell division and differentiation processes.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jorge Guio, Maria Teresa Bes, Monica Balsera, Laura Calvo-Begueria, Emma Sevilla, Maria Luisa Peleato, Maria F. Fillat
Summary: FurA is a multifunctional regulator in cyanobacteria that can develop disulfide reductase activity due to lack of a structural zinc ion. The interaction between FurA and the type m thioredoxin TrxA was confirmed through in vitro and in vivo studies, suggesting TrxA as a redox partner that modulates FurA performance in facilitating interactions with different ligands.
Article
Biochemistry & Molecular Biology
Ryo Nagao, Makio Yokono, Yoshifumi Ueno, Yoshiki Nakajima, Takehiro Suzuki, Ka-Ho Kato, Naoki Tsuboshita, Naoshi Dohmae, Jian-Ren Shen, Shigeki Ehira, Seiji Akimoto
Summary: Heterocysts are formed in filamentous heterocystous cyanobacteria under nitrogen-starvation conditions and have a lower amount of photosystem II (PSII) complexes compared to vegetative cells. Investigations on heterocysts have shown almost no PSII complexes but the presence of PSI complexes in the thylakoids. Analysis of fluorescence spectra and excitation-energy-relaxation processes in heterocysts revealed differences in fluorescence characteristics of PSII and PSI compared to vegetative thylakoids.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Microbiology
Qin-Xue Sun, Min Huang, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
Summary: This study identified a two-component signaling system, composed of CdgK and CdgS, that regulates cell size in cyanobacteria. The GGDEF domain of CdgS was found to be essential in cell size control. This research provides insights into the mechanisms of cell size regulation in cyanobacteria.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Wei-Yue Xing, Jing Liu, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
Summary: In this study, a new negative regulator called PatU3 was identified in the prokaryote Anabaena, which regulates cell division and heterocyst development. The results showed that PatU3 is a specific substrate of the protease activity of HetF, and HetF controls cell division and heterocyst differentiation by controlling the inhibitory effects of PatU3.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
T. V. Divya, Celin Acharya
Summary: In this study, we discovered a protein called AzuR that negatively regulates the metallothionein NmtA in cyanobacteria. We demonstrated that AzuR binds to the regulatory element of NmtA and that the presence of metal ions can disrupt this binding. Overexpression of AzuR in cyanobacteria increased their sensitivity to cadmium.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biology
Zi-Qian Wang, Yiling Yang, Ju-Yuan Zhang, Xiaoli Zeng, Cheng-Cai Zhang
Summary: A gene called trcR has been identified as a repressor for the transcription of the trn operon, which is activated under translational stress caused by antibiotics. Transcriptional and translational regulations play important roles in cell adaptation to environmental conditions.
COMMUNICATIONS BIOLOGY
(2023)
Article
Marine & Freshwater Biology
Zi-Qian Wang, Cheng-Cai Zhang
Summary: In this study, the authors demonstrate that the system responsible for modifying ANN-decoding tRNAs is involved in the sensitivity of cyanobacteria to the environmental neurotoxin BMAA. The study also reveals that both BMAA and inactivation of the t(6)A biosynthesis pathway affect translational fidelity and ribosome assembly. BMAA-resistant mutants screening is an effective method for gaining insight into the toxic mechanisms of BMAA.
AQUATIC TOXICOLOGY
(2022)
Article
Marine & Freshwater Biology
Manisha Banerjee, Prakash Kalwani, Dhiman Chakravarty, Beena Singh, Anand Ballal
Summary: The research revealed that low concentrations of selenium did not inhibit the growth and photosynthesis of the cyanobacterium, but higher concentrations led to substantial inhibition. Selenium also impacted the levels of endogenous reactive oxygen species (ROS) and the transcription of photosynthesis-related genes in the cyanobacterium.
AQUATIC TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Prashant Swapnil, Mukesh Meena, Ashwani K. Rai
Summary: Nitrate transport in cyanobacteria is mediated by ABC transporters, and GB can enhance the transport rate. The nrtA, nrtC, and nrtD proteins are crucial, with different types of molecular interactions between them.
Article
Microbiology
Umesh Pravin Dhuldhaj, Urja Vinodray Pandya
Summary: The present study showed that increasing As (III) concentration resulted in decreased growth, morphological changes, decreased nitrogenase activity, and altered enzyme activities and photosynthetic rates in the diazotrophic cyanobacterium Anabaena PCC 7120.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Akanksha Srivastava, Subhankar Biswas, Sandhya Yadav, Arvind Kumar, Hema Rajaram, Vaibhav Srivastava, Yogesh Mishra
Summary: Photosynthetic organisms, such as cyanobacteria, are negatively affected by cadmium (Cd) exposure, leading to the production of intracellular ROS and decreased photosynthetic performance. Cd exposure inactivates PSII reaction centers, disrupting energy transfer processes and decreasing linear electron flow efficiency. However, it induces an increase in PSI-based cyclic electron flow as a protective response against Cd-induced PSII damage in Anabaena sp. PCC 7120.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Ryo Nagao, Makio Yokono, Yoshifumi Ueno, Takehiro Suzuki, Koji Kato, Ka-Ho Kato, Naoki Tsuboshita, Tian-Yi Jiang, Naoshi Dohmae, Jian-Ren Shen, Shigeki Ehira, Seiji Akimoto
Summary: IsiAs are proteins expressed in cyanobacteria under iron-deficient conditions, surrounding the photosystem I trimer with a ring formation. The study found that the PSI-IsiA1 supercomplex is present in the wild-type under iron-replete conditions, while IsiA2 and IsiA3 bind to PSI monomer in the ΔisiA1 mutant under iron-deficient conditions. These findings provide insights into the multiple-expression system of the IsiA family in this cyanobacterium.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Biochemistry & Molecular Biology
Shota Shimizu, Haruya Ogawa, Naoki Tsuboshita, Takehiro Suzuki, Koji Kato, Yoshiki Nakajima, Naoshi Dohmae, Jian-Ren Shen, Ryo Nagao
Summary: In this study, we successfully purified PSI-monomer-PBS and PSI-dimer-PBS supercomplexes from iron-deficient cyanobacterium. This suggests that the binding between CpcL and PSI becomes tighter under iron deficiency, resulting in the formation of these two supercomplexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Biochemistry & Molecular Biology
Tingting Feng, Haitao Liu, Qinggang Xu, Jianzhong Sun, Haifeng Shi
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2015)
Article
Oncology
Ying Xu, Jianhua Jin, Wenbo Zhang, Zhi Zhang, Jiaming Gao, Qian Liu, Chenglin Zhou, Qinggang Xu, Haifeng Shi, Yongzhong Hou, Juanjuan Shi
Article
Chemistry, Physical
Jianxin Shou, Chunfang Jiang, Fang Wang, Muqing Qiu, Qinggang Xu
JOURNAL OF MOLECULAR LIQUIDS
(2015)
Article
Integrative & Complementary Medicine
Huimin Xu, Jianhua Jin, Lu Chen, Chunxiao Li, Qinggang Xu, Juanjuan Shi, Buchang Zhao, Yongzhong Hou, Hong Wang
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2016)
Article
Geriatrics & Gerontology
Y. Zhou, Q. Xu, Y. Dong, S. Zhu, S. Song, N. Sun
JOURNAL OF NUTRITION HEALTH & AGING
(2017)
Article
Biochemistry & Molecular Biology
Mengli You, Jianhua Jin, Qian Liu, QingGang Xu, Juanjuan Shi, Yongzhong Hou
JOURNAL OF CELLULAR BIOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Yue Zhou, Qinggang Xu, Xinghua Zhou, Shuang Song, Beiwei Zhu
Review
Immunology
Lulu Zuo, Ke Peng, Yihong Hu, Qinggang Xu
CURRENT HIV RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Qiushi Lin, Xuesong Chen, Fanzheng Meng, Kosuke Ogawa, Min Li, Ruipeng Song, Shugeng Zhang, Ziran Zhang, Xianglu Kong, Qinggang Xu, Fuliang He, Xuewei Bai, Bei Sun, Mien-Chie Hung, Lianxin Liu, Jack Wands, Xiaoqun Dong
Article
Oncology
Kosuke Ogawa, Qiushi Lin, Le Li, Xuewei Bai, Xuesong Chen, Hua Chen, Rui Kong, Yongwei Wang, Hong Zhu, Fuliang He, Qinggang Xu, Lianxin Liu, Min Li, Songhua Zhang, Katsuya Nagaoka, Rolf Carlson, Howard Safran, Kevin Charpentier, Bei Sun, Jack Wands, Xiaoqun Dong
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2019)
Article
Oncology
Feng Yu, Xiaoyan Wang, Hui Shi, Maorong Jiang, Jun Xu, Min Sun, Qinggang Xu, Frank Peprah Addai, Haifeng Shi, Jie Gu, Yang Zhou, Liqiong Liu
Summary: The research successfully developed CAR-T cells targeting HER2, which efficiently recognized and killed HER2-positive tumor cells in vitro, and significantly suppressed established ESCCs in vivo. The HER2.CAR-T cells showed promising immunotherapeutic potential for the treatment of HER2-positive esophageal cancer.
Review
Oncology
Xianggang Shi, Dongfeng Chen, Guodong Liu, Hailing Zhang, Xiaoyan Wang, Zhi Wu, Yan Wu, Feng Yu, Qinggang Xu
Summary: Elastin-like polypeptides (ELPs) are stimulus-responsive artificially designed proteins that have been widely studied and applied in various fields including protein purification, drug delivery, tissue engineering, and tumor therapy. This review focuses on the specific application of ELPs in cancer therapy, particularly the latest developments in the combined use of ELPs with photosensitizers. ELPs have shown great potential as drug carriers, enabling improved pharmacokinetic properties and targeted drug delivery while reducing cytotoxicity. The combination of ELPs with therapeutic drugs, antibodies, and photosensitizers has shown promising results in tumor therapy.
Article
Medicine, General & Internal
Lulu Zuo, Kai Liu, Honglian Liu, Yihong Hu, Zhijie Zhang, Jianru Qin, Qinggang Xu, Ke Peng, Xia Jin, Jian-Hua Wang, Chiyu Zhang
Letter
Medicine, General & Internal
Lulu Zuo, Jianru Qin, Qinggang Xu, Chiyu Zhang
Article
Oncology
Wenbo Zhang, Ying Xu, Qinggang Xu, Haifeng Shi, Juanjuan Shi, Yongzhong Hou