Article
Agriculture, Dairy & Animal Science
Derek J. Matthews, Ryan C. Fries, Nicholas D. Jeffery, Sarah A. Hamer, Ashley B. Saunders
Summary: Trypanosoma cruzi infection causes cardiac damage in dogs, and cardiac magnetic resonance imaging (CMR) can detect the damage. This study aimed to describe CMR in naturally infected dogs with chronic Chagas disease and evaluate the frequency of abnormalities in CMR and other diagnostic tests. The results suggest that CMR can provide useful information and support the use of naturally infected dogs as an animal model for Chagas disease.
Article
Immunology
Camila Victoria Sousa Oliveira, Oscar Moreno-Loaiza, Daniel Figueiredo-Vanzan, Isalira Peroba Ramos, Hilton Mata-Santos, Marcelo Torres Bozza, Claudia Neto Paiva, Emiliano Medei
Summary: This study investigated the role of IL-1 beta in chronic chagasic cardiomyopathy (CCC) using a mouse model. The results showed that the absence of functional IL-1 beta/IL-1R signaling did not prevent or reverse the decrease of cardiac function and the incidence of arrhythmias induced by CCC. Therefore, ruling out the IL-1 beta signaling pathway is an important step to discourage further attempts of IL-1 beta blockade as a therapeutic measure for CCC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Jose Mateus, Paola Nocua, Paola Lasso, Manuel Carlos Lopez, M. Carmen Thomas, Adriana Egui, Claudia Cuervo, John Mario Gonzalez, Concepcion J. Puerta, Adriana Cuellar
Summary: The study investigates the relationship between parasite control and immune T cell responses in Chagas disease by using distinct infection approaches in an animal model. Different infection outcomes were observed in single or mixed T. cruzi infection with different genotypes, leading to differential immune CD8(+) T cell responses. This suggests that the quality of the CD8(+) T cell response may dictate differences in infection outcomes during chronic T. cruzi infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Alessandro Marins-Dos-Santos, Jackline de Paula Ayres-Silva, Dina Antunes, Carlos Jose de Carvalho Moreira, Marcelo Pelajo-Machado, David Alfaro, Agustin G. Zapata, Adriana Cesar Bonomo, Wilson Savino, Juliana de Meis, Desio Aurelio Farias-de-Oliveira
Summary: This study demonstrates that Trypanosoma cruzi causes high tissue parasitism during the acute phase of Chagas disease. In mice with acute oral infection, the bone marrow cells are highly parasitized, particularly in the perivascular, intravascular, and near bone regions. The infection leads to a decrease in hematopoietic cells in the bone marrow, except for an increase in hematopoietic stem and progenitor cells. The study also suggests that the spleen may play a role in emergency hematopoiesis during acute T. cruzi infection. Overall, this research provides important insights into the impact of T. cruzi infection on the hematopoietic system.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Priscila Silva Grijo Farani, Khodeza Begum, Glaucia Vilar-Pereira, Isabela Resende Pereira, Igor C. Almeida, Sourav Roy, Joseli Lannes-Vieira, Otacilio Cruz Moreira
Summary: Research explored the immune response-related gene expression changes in heart tissues of C57BL/6 mice chronically infected with T. cruzi and treated with benznidazole (Bz) or Bz+pentoxifylline (PTX). The study found that treatment could mitigate the Th1-driven response in cardiac remodeling processes of CCC patients by restoring the expression of genes related to inflammatory response, cellular development, growth, proliferation, and tissue development pathways.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Nandadeva Lokugamage, Imran H. Chowdhury, Ronald J. Biediger, Robert Market, Sayadeth Khounlo, Navin D. Warier, Shen-An Hwang, Jeffrey K. Actor, Darren G. Woodside, Upendra Marathi, Peter Vanderslice, Nisha Jain Garg
Summary: 7HP349 is a small molecule activator that enhances the efficacy of DNA subunit vaccines in a model of Chagas disease. It improves control of acute parasite burden, reduces inflammatory infiltrate, improves tissue integrity, and controls tissue fibrosis in the heart and skeletal muscle. Additionally, 7HP349 increases splenic frequencies of CD8(+)T effector cells and enhances production of IFN-gamma and cytolytic molecules by CD4(+) and CD8(+) effector and central memory subsets in response to challenge infection.
Article
Immunology
Kristyn A. Hoffman, Maria Jose Villar, Cristina Poveda, Maria Elena Bottazzi, Peter J. Hotez, David J. Tweardy, Kathryn M. Jones
Summary: The study investigated the role of STAT3 in cardiac pathology during CCC, finding that its inhibition eliminated cardiac fibrosis but increased inflammation. This suggests that STAT3 plays a critical role in the cardiac inflammatory-fibrotic axis in CCC.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Natalia Lins da Silva-Gomes, Leonardo Alexandre de Souza Ruivo, Claudia Moreira, Marcelo Meuser-Batista, Cristiane Franca da Silva, Denise da Gama Jaen Batista, Stenio Fragoso, Gabriel Melo de Oliveira, Maria de Nazare Correia Soeiro, Otacilio C. Moreira
Summary: In this study, genetically modified strains of Trypanosoma cruzi were used to evaluate the role of NTPDases in parasite infectivity. The results showed that parasites overexpressing TcNTPDase-1 had higher infectivity, while hemi-knockout parasites had lower infectivity and no significant electrocardiographic changes. These findings highlight the potential of NTPDases as a therapeutic target for Chagas disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Izadora Volpato Rossi, Maria Alice Ferreira Nunes, Bruna Sabatke, Hennrique Taborda Ribas, Sheila Maria Brochado Winnischofer, Augusto Savio Peixoto Ramos, Jameel Malhador Inal, Marcel Ivan Ramirez
Summary: Chagas disease is caused by Trypanosoma cruzi, which uses blood-feeding triatomine bugs to infect mammalian hosts. The parasite needs to evade the complement system and quickly invade cells for infection, achieved through the expression of different molecules and release of extracellular vesicles (EVs). Selecting a population of T. cruzi after exposure to human serum stress factors can lead to increased metacyclogenesis, resistance to complement-mediated lysis, and greater infectivity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Jose A. Perez-Molina, Clara Crespillo-Andujar, Elena Trigo, Sandra Chamorro, Marta F. Arsuaga, Leticia F. Olavarrieta, Beatriz Navia, Oihane L. Martin, Begona L. Monge-Maillo, Francesca Norman, Val L. Lanza, Sergio Serrano-Villar
Summary: Chagas disease (CD) is a neglected disease with significant knowledge gaps regarding its pathogenesis, treatment, and prognostic factors. In this study, we investigated the changes in gut microbiota of CD patients and identified potential disease biomarkers.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Computer Science, Information Systems
Nidiyare Hevia-Montiel, Paulina Haro, Leonardo Guillermo-Cordero, Jorge Perez-Gonzalez
Summary: This research demonstrates the effectiveness and accuracy of deep learning methods based on the U-Net convolutional network architecture in the segmentation of histological images for Chagas disease.
Article
Immunology
Magali C. Girard, Micaela S. Ossowski, Arturo Munoz-Calderon, Marisa Fernandez, Yolanda Hernandez-Vasquez, Raul Chadi, Karina A. Gomez
Summary: This study investigated the potential alterations of Breg cells in CCD patients upon in vitro stimulation. The results showed that T. cruzi stimulation led to changes in the distribution and IL-10 production of B cells, with patients with cardiomyopathy exhibiting different immune phenotypes. Additionally, B cells from patients with cardiomyopathy displayed higher IL-17 secretion levels upon T. cruzi activation, and IL-10-independent mechanisms may be involved in controlling inflammation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Cell Biology
Xiang Nie, Jiahui Fan, Huihui Li, Jin Wang, Rong Xie, Chen Chen, Dao Wen Wang
Summary: This study suggests that dysregulation of cardiac circRNAs is an important characteristic of viral myocarditis, and is strongly correlated with immune response. The dysregulation of circRNAs in different mouse strains is associated with varying degrees of cardiac dysfunction.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Gabriela Specker, Damian Estrada, Rafael Radi, Lucia Piacenza
Summary: This study demonstrates the protective effects of Trypanosoma cruzi mitochondrial peroxiredoxin (MPX) in macrophage infections and in response to nifurtimox (NFX) toxicity. MPX content increased after NFX treatment and overexpression of MPX enhanced resistance to the drug. MPX exhibits holdase activity and its overexpression was observed in temperature-challenged parasites. These findings suggest a protective role of MPX holdase activity against NFX toxicity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Albany Resendiz-Mora, Giovanna Barrera-Aveleida, Anahi Sotelo-Rodriguez, Ivan Galarce-Sosa, Irene Nevarez-Lechuga, Juan Carlos Santiago-Hernandez, Benjamin Nogueda-Torres, Sergio Meza-Toledo, Saul Gomez-Manzo, Isabel Wong-Baeza, Isabel Baeza, Carlos Wong-Baeza
Summary: Chagas disease, caused by Trypanosoma cruzi, is a major public health problem endemic in Latin America and emerging globally. Current treatments, Benznidazole and Nifurtimox, have limited efficacy in the chronic phase. This study synthesized a non-polar molecule, B-NIPOx, which was shown to have higher trypanocidal activity than the reference drugs, reduced blood parasitemia and amastigote nests in infected mice, and prevented the development of Chagasic enteropathy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)