Article
Oncology
Mateusz Kozlowski, Dominika Borzyszkowska, Justyna Mirko, Agnieszka Turon-Skrzypinska, Katarzyna Piotrowska, Aleksandra Toloczko-Grabarek, Sebastian Kwiatkowski, Maciej Tarnowski, Iwona Rotter, Aneta Cymbaluk-Ploska
Summary: This study suggests that PDGF-AB, PDGF-BB, TGF-alpha, EGF, and ANG-2 may serve as potential diagnostic markers for endometrial cancer due to their significantly higher serum levels in endometrial cancer patients compared to those with non-cancerous endometrial lesions.
Article
Multidisciplinary Sciences
Qiuyan Ma, Jacob R. Beal, Arpita Bhurke, Athilakshmi Kannan, Jie Yu, Robert N. Taylor, Indrani C. Bagchi, Milan K. Bagchi
Summary: This study investigates the communication mechanisms of decidual cells in the uterus and reveals that they secrete extracellular vesicles (EVs) during decidualization. These EVs carry various proteins that promote endometrial differentiation, angiogenesis, and trophoblast development. Importantly, the EVs also facilitate the differentiation of trophoblast stem cells into extravillous trophoblasts.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Chao Chen, Jing Gao, Dan Chen, Jinyu Liu, Biwei He, Yan Chen, Huijuan Zhang, Xingyu Yang, Weiwei Cheng
Summary: In this study, it was found that miR-4443 was significantly increased in the placentas of severe preeclamptic patients, and upregulation of miR-4443 inhibited the migration and invasion of HTR-8/SVneo cells. The inhibitory effects of miR-4443 were mediated by the suppression of MMP2. This study suggests that miR-4443 may play an important role in the pathogenesis of preeclampsia through the HB-EGF/EGFR pathway.
Article
Obstetrics & Gynecology
Mikihiro Yoshie, Kensuke Ohishi, Gen Ishikawa, Atsuya Tsuru, Kazuya Kusama, Mana Azumi, Kazuhiro Tamura
Summary: This study investigates the role of the small GTP-binding protein Rap1 in EGF and HB-EGF-stimulated EVT invasion. Rap1 is expressed in EVTs, villous cytotrophoblasts, and syncytiotrophoblasts in the placenta. EGF and HB-EGF activate Rap1 and promote invasion, while Rap1 knockdown inhibits these effects and reduces EGFR protein level.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hsien-Ming Wu, Liang-Hsuan Chen, Hong-Yuan Huang, Hsin-Shih Wang, Chia-Lung Tsai
Summary: EGF and GnRH-II play essential roles in embryo implantation and early pregnancy by promoting the motility of human decidual endometrial stromal cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Yali Cheng, Liying Xie, Zhiying Xu, Mengxin Hao, Bingyi Yang, Weiwei Shan, Yiqin Wang, Qiaoying Lv, Xiaojun Chen
Summary: Progestin treatment for endometrial cancer has been unsatisfactory, but studies have shown that normal endometrial stromal cells can enhance the inhibitory effect of progestin by secreting neuron cell adhesion molecule (NrCAM), suggesting a new treatment strategy.
CANCER GENE THERAPY
(2022)
Article
Endocrinology & Metabolism
Ellen Menkhorst, Teresa So, Kate Rainczuk, Siena Barton, Wei Zhou, Tracey Edgell, Evdokia Dimitriadis
Summary: A healthy pregnancy requires successful blastocyst implantation into an adequately prepared endometrium. The decidualization of uterine endometrial stromal fibroblast cells (hESFs) is critical for a healthy pregnancy. This study investigates how decidualization affects miR release and the function of one miR, miR-19b-3p, which has been associated with recurrent pregnancy loss.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anastasiia S. Boiko, Irina A. Mednova, Elena G. Kornetova, Nikolay A. Bokhan, Svetlana A. Ivanova
Summary: The study aimed to determine the serum concentration of six growth factors in schizophrenia patients and their correlations with clinical characteristics. The results showed significant elevation in TGF-a and PDGF-AA levels in schizophrenia patients, and a positive correlation between FGF-2 level and the duration of schizophrenia. The PANSS total score was positively correlated with FGF-2 level and negatively correlated with TGF-a level. These results suggest the involvement of growth factors in the development of schizophrenia and the need for combined biomarker screening for improved diagnosis and clinical follow-up.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yifat Koren Carmi, Hazem Khamaisi, Rina Adawi, Eden Noyman, Jacob Gopas, Jamal Mahajna
Summary: Ovarian cancer is the second most common gynecological malignancy with poor survival rates. Chemoresistance, a major obstacle in treating ovarian cancer, is influenced by tumor microenvironment and soluble factors. FDA-approved JAK inhibitors can partially restore platinum sensitivity in ovarian cancer cells in the tumor microenvironment. Crizotinib, in combination with platinum, can block the promotion of platinum resistance by HGF and restore platinum sensitivity in ovarian cancer cells. Additionally, 2HE2 can restore platinum sensitivity in ovarian cancer cells exposed to the tumor microenvironment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
P. Deryabin, J. S. Ivanova, A. Borodkina
Summary: Successful implantation requires a finely tuned dialog between the invading embryo and the maternal endometrium. Premature senescence of endometrial stromal cells (EnSC) can lead to defective decidual transformation and impaired endometrial-blastocyst interaction. Senescent EnSC exhibit elevated intracellular reactive oxygen species (ROS) levels, originating from mitochondrial dysfunction and insufficient antioxidant defense. This imbalance in ROS release affects neighboring trophoblast-like cells, leading to DNA damage and potentially impacting embryo migration and invasion.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Suqin Zhu, Dianliang Lin, Zhoujie Ye, Xiaojing Chen, Wenwen Jiang, Huiling Xu, Song Quan, Beihong Zheng
Summary: Endometrial decidualization is crucial for maintaining pregnancy, and GOLPH3 plays a role in regulating gene expression and alternative splicing in human endometrial stromal cells. Through bioinformatics analysis, it was found that GOLPH3 affects the transcriptional level and alternative splicing events in hESCs, particularly in pathways related to endometrial decidualization.
Article
Developmental Biology
Upendra Kumar Soni, Sangappa Basanna Chadchan, Rakesh Kumar Gupta, Vijay Kumar, Rajesh Kumar Jha
Summary: miR-149 plays a crucial role in regulating endometrial receptivity and decidualization for trophoblast implantation by targeting PARP-2 and caspase-8 proteins in endometrial cells.
MOLECULAR HUMAN REPRODUCTION
(2021)
Article
Pharmacology & Pharmacy
Rosario Macchi, Agustina D. Sotelo, Andrea C. Parrado, Luciana S. Salaverry, Guillermo A. Blanco, Marisa S. Castro, Estela B. Rey-Roldan, Andrea M. Canellada
Summary: During implantation in humans, there is a deep interaction between the endometrium and the invading trophoblast. The AT1 receptor is involved in the reciprocal dialogue occurring between endometrial stroma and trophoblast cells. Angiotensin II (Ang II) can promote endometrial decidualization and secretion of CXCL8 and MMP-2 in human endometrial stromal cells. Treatment with losartan, an AT1 receptor blocker, can inhibit the effects of Ang II on T-HESC and trophoblast migration.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2023)
Article
Obstetrics & Gynecology
Serhat Toprak, Eda Adeviye Sahin, Hanifi Sahin, Yusuf Aytac Tohma, Ercan Yilmaz, Mehmet Mutlu Meydanli
Summary: Lymphovascular space invasion, primary tumor diameter of at least 3 cm, and at least 50% deep myometrial invasion are independent predictors of cervical stromal involvement in patients with endometrioid-type endometrial cancer.
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS
(2021)
Article
Biology
Bo Lv, Xiaoyu Xu, Xunyi Zhang, Lingbin Qi, Wen He, Lu Wang, Xian Chen, Luying Peng, Jinfeng Xue, Yazhong Ji, Zhigang Xue
Summary: Human endometrial stromal cells show differential gene expression related to blood vessel development and female pregnancy after coculture with embryos, indicating the initiation of blood vessel development at the implantation stage. Key pathways including epithelial cell development and oxygen response are involved in the signaling processes during this stage. The interaction between EmSCs and embryonic trophoblasts involves integrins and fibroblast growth factor pathways.
Review
Biochemical Research Methods
Wiebke Jahr, Philipp Velicky, Johann Georg Danzl
Article
Anesthesiology
Peter Wohlrab, Stefan Boehme, Christoph Kaun, Johann Wojta, Andreas Spittler, Leila Saleh, Martin Knoefler, Klaus Markstaller, Klaus U. Klein, Verena Tretter
ANESTHESIA AND ANALGESIA
(2020)
Article
Biochemistry & Molecular Biology
Andras Szilagyi, Zsolt Gelencser, Roberto Romero, Yi Xu, Peter Kiraly, Amanda Demeter, Janos Palhalmi, Balazs A. Gyorffy, Kata Juhasz, Petronella Hupuczi, Katalin Adrienna Kekesi, Gudrun Meinhardt, Zoltan Papp, Sorin Draghici, Offer Erez, Adi Laurentiu Tarca, Martin Knoefler, Nandor Gabor Than
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Reproductive Biology
Hanns Helmer, Leila Saleh, Ljubomir Petricevic, Martin Knoefler, Torsten M. Reinheimer
BIOLOGY OF REPRODUCTION
(2020)
Article
Multidisciplinary Sciences
Gudrun Meinhardt, Sandra Haider, Victoria Kunihs, Leila Saleh, Juergen Pollheimer, Christian Fiala, Szabolcs Hetey, Zsofia Feher, Andras Szilagyi, Nandor Gabor Than, Martin Knoefler
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Neurosciences
Kohgaku Eguchi, Philipp Velicky, Elena Hollergschwandtner, Makoto Itakura, Yugo Fukazawa, Johann Georg Danzl, Ryuichi Shigemoto
FRONTIERS IN CELLULAR NEUROSCIENCE
(2020)
Article
Obstetrics & Gynecology
Karin Windsperger, Sigrid Vondra, Andreas Ian Lackner, Victoria Kunihs, Peter Haslinger, Gudrun Meinhardt, Bianca Dietrich, Sabine Dekan, Christian Fiala, Martin Knofler, Leila Saleh, Jurgen Pollheimer
HUMAN REPRODUCTION
(2020)
Article
Reproductive Biology
Elisa T. Zhang, Roberta L. Hannibal, Keyla M. Badillo Rivera, Janet H. T. Song, Kelly McGowan, Xiaowei Zhu, Gudrun Meinhardt, Martin Knoefler, Juergen Pollheimer, Alexander E. Urban, Ann K. Folkins, Deirdre J. Lyell, Julie C. Baker
Summary: The study identified misregulated gene expression patterns in tissues from placenta previa and percreta compared with control cases, with upregulated genes predominantly marking extravillous trophoblasts. PRG2 and AQPEP protein levels were found to be upregulated in both conditions, with AQPEP restricted to trophoblasts and PRG2 mislocalized throughout the fetal membranes. This suggests a signature of previa and PAS in the extraembryonic tissues surrounding the conceptus, reflecting increased trophoblast invasiveness.
BIOLOGY OF REPRODUCTION
(2021)
Article
Engineering, Biomedical
Abigail J. Deloria, Sandra Haider, Bianca Dietrich, Victoria Kunihs, Simon Oberhofer, Martin Knofler, Rainer Leitgeb, Mengyang Liu, Wolfgang Drexler, Richard Haindl
Summary: The study demonstrates the use of 3D optical coherence tomography (OCT) for analyzing human placenta organoids without sample preparation. Results show that 3D OCT enables detailed monitoring of placenta organoids, visualizing and quantifying internal structures in situ to assess differentiation status and aid advancement in the field.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Bianca Dietrich, Sandra Haider, Gudrun Meinhardt, Juergen Pollheimer, Martin Knoefler
Summary: This article discusses two important signaling pathways, WNT and NOTCH, in the development of human placenta and trophoblasts. The WNT pathway is necessary for the expansion of trophoblast progenitors and stem cells, while NOTCH1 is required for the proliferation and survival of extravillous trophoblast precursors.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Sandra Haider, Andreas Ian Lackner, Bianca Dietrich, Victoria Kunihs, Peter Haslinger, Gudrun Meinhardt, Theresa Maxian, Leila Saleh, Christian Fiala, Juergen Pollheimer, Paulina A. Latos, Martin Knoefler
Summary: Comparative gene expression analysis revealed the importance of transforming growth factor-beta (TGF-beta) signaling in the development of extravillous trophoblasts (EVTs), particularly in the differentiation of placental EVTs into decidual EVTs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Soma Ray, Abhik Saha, Ananya Ghosh, Namrata Roy, Ram P. Kumar, Gudrun Meinhardt, Abhirup Mukerjee, Sumedha Gunewardena, Rajnish Kumar, Martin Knoefler, Soumen Paul
Summary: WWTR1 is identified as a master regulator of trophoblast fate choice during human placentation, promoting CTB self-renewal and EVT differentiation while preventing STB induction. The fine-tuning of trophoblast fate by WWTR1 is achieved through direct regulation of WNT signaling components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Sigrid Vondra, Anna-Lena Hobler, Andreas Ian Lackner, Johanna Raffetseder, Zala Nikita Mihalic, Andrea Vogel, Leila Saleh, Victoria Kunihs, Peter Haslinger, Markus Wahrmann, Heinrich Husslein, Raimund Oberle, Julia Kargl, Sandra Haider, Paulina Latos, Gernot Schabbauer, Martin Knofler, Jan Ernerudh, Jurgen Pollheimer
Summary: During pregnancy, different macrophage populations are found in the decidua basalis (decB) and decidua parietalis (decP), with decBAMs being CD11chi and efficient inducers of Tregs, while decPAMs exhibit a dendritic cell-like phenotype, enhanced phagocytosis, and the ability to activate T cells. Strikingly, EVT-conditioned media can convert decPAMs into a decBAM phenotype, highlighting the critical role of extravillous trophoblasts (EVTs) in the induction of decB-associated macrophage polarization.
Article
Developmental Biology
Bianca Dietrich, Victoria Kunihs, Juergen Pollheimer, Martin Knoefler, Sandra Haider
Summary: 3D trophoblast organoids (TB-ORG) are a reliable model for studying extravillous trophoblast (EVT) lineage formation and differentiation. However, alternative cell sources are needed due to limited access to first trimester placentae. In this study, we tested whether commonly used trophoblastic cell lines, ACH-3P, HTR-8/SVneo, and SWAN-71, could self-organize into organoids and differentiate into EVT. Only ACH-3P formed organoids resembling TB-ORG architectures, and induction of EVT differentiation resulted in the formation of HLA-Gpos areas. Therefore, ACH-3P-ORGs provide another model for studying controlled EVT lineage formation and differentiation.
Article
Cell Biology
Gael Castel, Dimitri Meistermann, Betty Bretin, Julie Firmin, Justine Blin, Sophie Loubersac, Alexandre Bruneau, Simon Chevolleau, Stephanie Kilens, Caroline Chariau, Anne Gaignerie, Quentin Francheteau, Harunobu Kagawa, Eric Charpentier, Lea Flippe, Valentin Francois--Campion, Sandra Haider, Bianca Dietrich, Martin Knoefler, Takahiro Arima, Jeremie Bourdon, Nicolas Rivron, Damien Masson, Thierry Fournier, Hiroaki Okae, Thomas Freour, Laurent David