Article
Biochemistry & Molecular Biology
Yuri Prozzillo, Stefano Cuticone, Diego Ferreri, Gaia Fattorini, Giovanni Messina, Patrizio Dimitri
Summary: The chromatin organization in Drosophila melanogaster is regulated by Tip60 chromatin remodeling complex (dTip60), DOM-A.C and DOM-B.C complexes, which share most subunits. This study combined genetics and cell biology to investigate the biological roles of these subunits in Drosophila melanogaster development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Gaorui Gong, Yang Xiong, Shijun Xiao, Xi-Yin Li, Peipei Huang, Qian Liao, Qingqing Han, Qiaohong Lin, Cheng Dan, Li Zhou, Fan Ren, Qi Zhou, Jian-Fang Gui, Jie Mei
Summary: We assembled highly homomorphic Y and X chromosomes in yellow catfish and discovered that the Y chromosome is at an early stage of differentiation with no clear evidence of evolutionary strata and recombination suppression. The accumulation of sex-antagonistic mutations and repetitive elements in the sex-determining region suggests their role in recombination suppression between young X and Y chromosomes. Additionally, we found distinct chromatin organizations and nuclear spatial organization in the YY supermales and XX females, indicating chromatin remodeling after sex reversal.
NATIONAL SCIENCE REVIEW
(2023)
Article
Biochemistry & Molecular Biology
Virender K. Sahota, Aelfwin Stone, Nathaniel S. Woodling, Jereme G. Spiers, Joern R. Steinert, Linda Partridge, Hrvoje Augustin
Summary: Alterations in the neuromuscular system contribute to various diseases and aging-related muscle loss. This study identifies Plum as a modulator of MYO function in the neuromuscular system and reveals its role in regulating muscle size and synaptic function.
Article
Multidisciplinary Sciences
Haofeng Wang, Yunjie Xiao, Xia Chen, Mengwen Zhang, Guangxin Sun, Feng Wang, Lin Wang, Hanxiao Zhang, Xiaoyu Zhang, Xin Yang, Wenling Li, Yi Wei, Deqiang Yao, Bing Zhang, Jun Li, Wen Cui, Fenghua Wang, Cheng Chen, Wei Shen, Dan Su, Fang Bai, Jinhai Huang, Sheng Ye, Lei Zhang, Xiaoyun Ji, Wei Wang, Zefang Wang, Mark Hochstrasser, Haitao Yang
Summary: This study reveals the importance of interface residues in the binding of CI factors CidA and CidB, which contributes to the diversity of cytoplasmic incompatibility. Sequence divergence in CidA and CidB proteins may explain the complex incompatibility patterns observed in mosquitoes infected with different Wolbachia strains.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Ines Schoberleitner, Birte Mertens, Ingo Bauer, Alexandra Lusser
Summary: The ATP-dependent chromatin remodeling factor CHD1 plays a crucial role in the incorporation of the histone variant H3.3 in the fly brain, affecting sensory perception genes and leading to defects in olfactory and gustatory response behavior. Additionally, Chd1-mutant flies exhibit shortened lifespan and indications of premature functional aging, including impaired negative geotaxis and exploratory walking. Neuronal re-expression of Chd1 rescues the exploratory walking phenotype but not the olfactory defects.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Marie-Line Faucillion, Anna-Mia Johansson, Jan Larsson
Summary: The stability of RNA plays a crucial role in achieving balanced gene expression. It can respond to changes in transcription by shifting the RNA stability in the opposite direction to counteract gene mis-expression, and it can also enhance differential gene expression between individuals by adjusting the RNA stability in the same direction as transcriptional changes. These mechanisms apply to both housekeeping and non-housekeeping genes and are observed in both flies and mammals.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Elisa Mascolo, Francesco Liguori, Lorenzo Stufera Mecarelli, Noemi Amoroso, Chiara Merigliano, Susanna Amadio, Cinzia Volonte, Roberto Contestabile, Angela Tramonti, Fiammetta Verni
Summary: In this study, silencing of Hex-A and Hex-C in Drosophila resulted in a MODY-2-like hyperglycemia phenotype. Hex-A is crucial for insulin secretion and required for Hex-C expression. Reduced levels of either Hex-A or Hex-C led to chromosome aberrations and increased production of advanced glycation end-products (AGEs) and reactive oxygen species (ROS). Treating GCK-depleted larvae with the antioxidant vitamin B6 rescued chromosome aberrations, while treatment with a B6 inhibitor enhanced genomic instability, suggesting a link between MODY-2 and genome integrity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Beatrice Horard, Kevin Terretaz, Anne-Sophie Gosselin-Grenet, Helene Sobry, Mathieu Sicard, Frederic Landmann, Benjamin Loppin
Summary: Wolbachia, a type of endosymbiotic bacteria, manipulates the reproduction of arthropods through cellular mechanisms. CidA and CidB, two Cif proteins, play a crucial role in germ cells of fruit flies and mosquitoes. CidB disrupts paternal DNA replication, leading to incomplete replication of paternal chromosomes and causing infertility. This study reveals the function of CI as a transgenerational toxin-antidote system.
Article
Biochemistry & Molecular Biology
Ekaterina Proshkina, Elena Yushkova, Liubov Koval, Nadezhda Zemskaya, Evgeniya Shchegoleva, Ilya Solovev, Daria Yakovleva, Natalya Pakshina, Natalia Ulyasheva, Mikhail Shaposhnikov, Alexey Moskalev
Summary: Small RNAs play a crucial role in coordinating various cellular processes, affecting gene expression, genomic stability, and suppressing mutagenic transposon activity, thus influencing aging, longevity, and sensitivity to stress factors. Proteins of the Argonaute family are essential for the biogenesis and activity of small RNAs, but their role in regulating lifespan and radioresistance is still not well understood. Studies on knockdown of Argonaute genes in different tissues of Drosophila melanogaster show tissue-specific effects on lifespan, survival after irradiation, and retrotransposon levels, providing insights into the associated molecular mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Hae-Ri Lee, Seung-Jip Yang, Hyun-Kyung Choi, Jin-A Kim, Il-Hoan Oh
Summary: Regulation of CHD1 expression levels hierarchically determines the primitive state of MSCs and their ability to support stem cells, ultimately influencing tissue regeneration.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Genetics & Heredity
Aaron T. Crain, Stephen Klusza, Robin L. Armstrong, Priscila Santa Rosa, Brenda R. S. Temple, Brian D. Strahl, Daniel J. McKay, A. Gregory Matera, Robert J. Duronio
Summary: KMT5A and Set8 enzymes have functional conservation in Drosophila development, and the N-terminal domain of Set8 has a specific role in eye development, which is distinct from H4K20me1.
Article
Genetics & Heredity
Oleg Bylino, Airat N. Ibragimov, Anna E. Pravednikova, Yulii Shidlovskii
Summary: Chromosome conformation capture methods (C-methods) are important tools for analyzing the spatial interactions between DNA regions. Understanding the basic steps of the chromatin conformation capture procedure (3C) is crucial, and optimizing conditions for cell treatment can lead to more efficient experiments.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Jordi Bernues, Andrea Izquierdo-Boulstridge, Oscar Reina, Lucia Castejon, Elena Fernandez-Castaner, Nuria Leal, Nancy Guerrero-Pepinosa, Carles Bonet-Costa, Olivera Vujatovic, Paula Climent-Canto, Fernando Azorin
Summary: Post-translational modifications of core histones, such as dH1K27me2, play crucial roles in epigenetic regulation of chromatin. In Drosophila, dH1K27me2 is a major modification of heterochromatin and contributes to heterochromatin organization independently of H3K9 methylation.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Hai T. Dao, Linh T. D. Pham
Summary: Structural and biochemical studies have shown that the nucleosome acidic patch plays a crucial role in regulating the function of nuclear proteins. Different remodeler families interact with the acidic patch in distinct ways, leading to specific remodeling outcomes. The impact of cancer-related histone mutations on nucleosome remodeling is not well understood. Recent advancements in chromatin reconstitution have shed light on the effects of acidic patch mutations on chromatin structure.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Biology
Evelina Charidemou, Maria A. Tsiarli, Andria Theophanous, Vural Yilmaz, Chrysoula Pitsouli, Katerina Strati, Julian L. Griffin, Antonis Kirmizis
Summary: The study demonstrates that NAA40 regulates cellular acetyl-CoA levels, thereby impacting lipid synthesis and insulin signaling. This reveals a novel pathway through which histone-modifying enzymes influence cellular metabolism and has potential implications in metabolic disorders.