Review
Biochemistry & Molecular Biology
Marie Berenguer, Gregg Duester
Summary: The role of retinoic acid (RA) and Meis1/2 in limb development is complex, with RA activating Meis1/2 in the proximal limb bud mesoderm while FGF8 represses Meis1/2 in the distal limb to generate proximodistal patterning.
Article
Oncology
Cedrik Labreche, David P. Cook, John Abou-Hamad, Julia Pascoal, Benjamin R. Pryce, Khalid N. Al-Zahrani, Luc A. Sabourin
Summary: Breast cancer is a highly heterogeneous disease with multiple drivers and complex regulatory networks. Postn plays a crucial role in tumor development, with about 50% of breast tumors acquiring Postn expression in epithelial tumor cells. The regulation of Postn expression involves cross-talk between FGFR, TGF beta, and PI3K/AKT pathways.
BREAST CANCER RESEARCH
(2021)
Article
Pathology
Jie Yu, Sarah L. Berga, Eric Zou, Andrew D. Schrepf, Daniel J. Clauw, Sawsan As-Sanie, Robert N. Taylor
Summary: Pelvic pain in women with endometriosis is related to neuroinflammation and nociceptor nerves. This study investigated the role of IL-10 in uterine nociception and found that NGF and BDNF and their receptors play a role in innervation of the endometrium. IL-10 was found to stimulate the expression of TrkA/B in endometrial stromal cells.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Multidisciplinary Sciences
Daltry L. Snider, Moonhee Park, Kristen A. Murphy, Dia C. Beachboard, Stacy M. Horner
Summary: The RNA-binding protein RIG-I plays a crucial role in initiating the innate immune response against viruses. In this study, the researchers found that a protein called UFL1, along with the process of ufmylation, promotes the induction of interferons in response to RIG-I activation. They also discovered that UFL1 interacts with the protein 14-3-3e at the cell membrane, and this complex is recruited to activated RIG-I to facilitate immune signaling. Furthermore, they found that ufmylation is necessary for the interaction between 14-3-3e and RIG-I, which is essential for downstream signal transduction and IFN induction.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Pharmacology & Pharmacy
Shujie Xu, Xiangyue He, Junzhu Shi, Ziguang Li, Jinhuan Song, Jingyun Wang, Guang Wang, Beate Brand-Saberi, Xin Cheng, Xuesong Yang
Summary: This study demonstrates that exposure to 10(-6) M Dex can lead to abnormal myogenesis in chicken embryos, mainly due to aberrant cell proliferation and changes in gene expression related to somite formation and differentiation. RNA sequencing revealed significant differential gene expressions in regulating myofibril development and systemic development, as well as dramatic alterations in retinoic acid (RA) signaling during somite development in chicken embryos exposed to Dex. Further experiments confirmed that Dex treatment affects RA signaling and is principally mediated by FGF-ERK signaling.
Article
Obstetrics & Gynecology
Rafiatu Azumah, Menghe Liu, Katja Hummitzsch, Nicole A. Bastian, Monica D. Hartanti, Helen F. Irving-Rodgers, Richard A. Anderson, Raymond J. Rodgers
Summary: This study investigates the changes in TGF beta signalling during foetal ovary development and its potential influence on the expression of PCOS candidate genes. The findings suggest that TGF beta 1 inhibits the expression of certain PCOS candidate genes in foetal ovarian fibroblasts, while increasing the expression of an AR cofactor. The study highlights the importance of TGF beta signalling in the development of PCOS.
HUMAN REPRODUCTION
(2022)
Article
Biology
Spenser S. Smith, Daniel Chu, Tiange Qu, Jessye A. Aggleton, Richard A. Schneider
Summary: This study uncovers the molecular mechanisms underlying jaw length development, demonstrating that differential regulation of TGF beta signaling and Mmp13 promoter structure play a critical role in avian jaw development and evolution.
Article
Biology
Dina Rekler, Chaya Kalcheim
Summary: This study reveals that retinoic acid synthesized in the neural tube regulates the end of neural crest development by inhibiting BMP signaling. The activity of neural crest-specific genes also inhibits retinoic acid synthesis in the roof plate. There is a mutual repressive interaction between neural crest and roof plate traits, compromising the cellular and molecular architecture of the roof plate.
Article
Biochemistry & Molecular Biology
Alexander J. Knights, Shanshan Liu, Yingxu Ma, Victoria S. Nudell, Eric Perkey, Matthew J. Sorensen, Robert T. Kennedy, Ivan Maillard, Li Ye, Heejin Jun, Jun Wu
Summary: This study demonstrates that macrophages play a crucial role in regulating thermogenesis by secreting acetylcholine in adipose tissue. The abundance of ChAMs increases in response to acute cold exposure, and their regulation is mediated through adrenergic signaling. Deletion of the enzyme ChAT impairs cold-induced thermogenesis in mice.
Article
Developmental Biology
Niveda Udaykumar, Mohd Ali Abbas Zaidi, Aishwarya Rai, Jonaki Sen
Summary: During embryonic development, defects in the invagination process of the forebrain roof plate, caused by CNKSR2 knockdown, result in middle interhemispheric holoprosencephaly (MIH-HPE) in humans. CNKSR2 functions by modulating the Ras/Raf/MEK signaling, crucial for patterning and invagination of the forebrain roof plate and the formation of the cerebral hemispheres. This study identifies a novel downstream mediator of retinoic acid signaling and sheds light on the molecular players involved in avian forebrain morphogenesis.
Article
Agriculture, Dairy & Animal Science
Gongyan Liu, Ce Liu, Yin Zhang, Haitao Sun, Liping Yang, Liya Bai, Shuxia Gao
Summary: The study revealed that skinning rabbits in winter resulted in better fur quality and hair follicle development compared to other seasons, as indicated by skin weight, area, thickness, hair follicle density, and various gene expressions related to growth factors and signaling pathways. Summer skinning had adverse effects on the rabbit coat, fat weight, and gene expressions, indicating a strong seasonal influence on rabbit physiology.
Article
Endocrinology & Metabolism
Blanca Rubio, Cristina Mora, Cristina Pintado, Lorena Mazuecos, Alejandro Fernandez, Virginia Lopez, Antonio Andres, Nilda Gallardo
Summary: Central leptin in lean rats regulates cardiac remodeling by balancing anti- and pro-hypertrophic programs through nutrient sensing pathways, involving PPAR beta/delta.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Article
Biochemistry & Molecular Biology
Ruiqi Liu, Hikaru Niimi, Minoru Ueda, Yousuke Takaoka
Summary: Ethylene and jasmonate are plant hormones that regulate plant development and defense mechanisms. They act synergistically against fungal infections, but antagonistically in response to wounds and apical hook formation. The coordination of these responses is mediated by protein-protein interactions between EIN3/EIL1 and MYC transcription factors. These factors are both activated by interactions with MED25, which upregulates downstream gene expression. The study found that MED25's short binding domain is responsible for the interaction with EIN3/EIL1, suggesting competition between the two transcription factors for binding with MED25. This finding deepens our understanding of the coordination between the ethylene and jasmonate regulatory systems.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Fisheries
Xinying Cao, Wuting Lu, Yang Gang, Baoqing Hu, Chungen Wen
Summary: In this study, the full-length cDNA of Prx5 was cloned from Cristaria plicata, and it was found to have antioxidant function and protect DNA from oxidative damage. Furthermore, Prx5 was involved in transcriptional regulation by interacting with the transcription factor MafK, and MafK could bind with Nrf2 to regulate the downstream target gene Prx5.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Fumihiro Fujiki, Soyoko Morimoto, Akiko Katsuhara, Akane Okuda, Saeka Ogawa, Eriko Ueda, Maki Miyazaki, Ayako Isotani, Masahito Ikawa, Sumiyuki Nishida, Hiroko Nakajima, Akihiro Tsuboi, Yoshihiro Oka, Jun Nakata, Naoki Hosen, Atsushi Kumanogoh, Yusuke Oji, Haruo Sugiyama
Summary: In this study, researchers identified a key molecule called retinol dehydrogenase 10 (Rdh10) that plays a crucial role in regulating T cell differentiation. They found that vitamin A metabolites activate retinoic acid receptor (RAR) signaling, leading to comprehensive epigenetic repression of memory T cell-associated genes and promoting effector T cell differentiation. Importantly, memory T cells generated through blocking RAR signaling showed strong anti-tumor responses in adoptive T cell transfer setting. This study highlights the importance of vitamin A metabolism and its signaling in memory T cell-based cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Jessica Cristina Marin-Llera, Jesus Chimal-Monroy
CELL BIOLOGY INTERNATIONAL
(2018)
Article
Cell & Tissue Engineering
C. I. Lorda-Diez, J. A. Montero, C. Sanchez-Fernandez, J. A. Garcia-Porrero, J. Chimal-Monroy, J. M. Hurle
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2018)
Article
Developmental Biology
Francisco Carmona-Aldana, Cecilia Zampedri, Fernando Suaste-Olmos, Adrian Murillo-de-Ozores, Georgina Guerrero, Rodrigo Arzate-Mejia, Ernesto Maldonado, Rosa E. Navarro, Jesus Chimal-Monroy, Felix Recillas-Targa
MECHANISMS OF DEVELOPMENT
(2018)
Review
Genetics & Heredity
Jessica Cristina Marin-Llera, David Garciadiego-Cazares, Jesus Chimal-Monroy
FRONTIERS IN GENETICS
(2019)
Article
Developmental Biology
Alejandro Farrera-Hernandez, Jessica Cristina Marin-Llera, Jesus Chimal-Monroy
Summary: This study revealed that WNT5A activates calcium (Ca2+) release in the undifferentiated region during digit development, and inhibiting Ca2+ release or calcineurin (CaN) or nuclear factor of activated T-cells (NFAT) functions leads to a suppression of cartilage differentiation. The non-canonical WNT5A-Ca2+-CaN-NFAT signaling pathway plays a crucial role in promoting chondrogenic signals and facilitating chondrogenesis during embryonic digit development.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jessica Cristina Marin-Llera, Carlos Ignacio Lorda-Diez, Juan Mario Hurle, Jesus Chimal-Monroy
Summary: In this study, two new early limb bud subpopulations of skeletal progenitors were identified based on the differential expression of the SCA-1 marker. These cell subpopulations have different osteogenic and chondrogenic capacities in response to inductive signals, with SCA-1 expression possibly playing a permissive role in cell fate determination during limb development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jessica Cristina Marin-Llera, Montse Fernandez-Calderon, Jesus Chimal-Monroy
Summary: Cell differentiation is the regulated process of cell commitment, which contributes to the formation of developing tissues and organs. Understanding the early steps of cell differentiation is crucial for understanding other complex processes. Recombinant chicken limbs provide an experimental model to study cell differentiation and pattern generation.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Multidisciplinary Sciences
Jessica Cristina Marin-Llera, Jesus Chimal-Monroy
Summary: During embryonic development, a variety of genetic programs are activated and regulated by morphogens, which lead to the generation of diverse somatic cells. The soaked bead assay is an experimental tool that can study the role of specific molecules in development, providing information on the control of cell induction, cell fate, and pattern formation.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Martha Elena Diaz-Hernandez, Claudio Ivan Galvan-Hernandez, Jessica Cristina Marin-Llera, Karen Camargo-Sosa, Marcia Bustamante, Sabina Wischin, Jesus Chimal-Monroy
Summary: The study investigates the initiation of cell death in the anterior margin of the limb to establish the ANZ through interactions of FGF, BMP, and WNT signaling. It demonstrates that the inhibition of WNT or FGF signaling, or the activation of BMP signaling, induces cell death in the anterior margin of the limb, with correlated regulation of Dkk, Fgf8, and Bmp4 expression. This work provides insights into the negative feedback loop between FGF8, BMP4, and DKK in controlling cell death in the anterior margin of the limb.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Roberto Damian Garcia-Garcia, Estefania Garay-Pacheco, Jessica Cristina Marin-Llera, Jesus Chimal-Monroy
Summary: Organ formation is regulated by cell commitment and gene transcription networks. Organoids can be used to study organ development, but the formation of skeletal elements in organoids is challenging. This article proposes using the recombinant limb assay system as an in vivo organoid model to study the formation of skeletal elements.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Jessica Cristina Marin-llera, Carlos Amaury Jimenez-cardenas, Jesus Chimal-Monroy
Summary: The molecular cascade of tendon formation involves the expression of the Scleraxis (Scx) gene by progenitor cells. Additional factors such as Mohawk (Mkx) and early growth response (Egr) are necessary for tendon commitment, maintenance, and differentiation. Inhibiting the wingless and int-related protein signaling induces Scx expression. Transforming growth factor-beta (TGF beta) plays a role in maintaining tendon cell fate. Understanding embryonic tendon development is important for improving therapeutic strategies in regenerative medicine.
Article
Developmental Biology
Valentina Garcia-Lee, Martha E. Diaz-Hernandez, Jesus Chimal-Monroy
Summary: Cell differentiation in the musculoskeletal system is highly coordinated during limb development, with WNT and FGF signals maintaining undifferentiated mesenchymal cells while TGF beta is closely involved in tendon differentiation. In this study, the inhibition of WNT signaling promoted Scx gene expression, and simultaneously blocking both WNT and TGF beta signals further enhanced Scx gene expression. These findings suggest that inhibition of WNT signaling is necessary and sufficient to induce Scx gene expression.
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Argelia S. Garcia-Cervera, Jesus Chimal-Monroy, Jessica C. Marin-Llera
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Sabina Wischin, Cristina Castaneda-Patlan, Martha Robles-Flores, Jesus Chimal-Monroy
Article
Cell Biology
Juan A. Montero, Carlos I. Lorda-Diez, Javier Francisco-Morcillo, Jesus Chimal-Monroy, Juan A. Garcia-Porrero, Juan M. Hurle
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2017)