Review
Chemistry, Physical
Xiao Wang, Ming-Zhu Lu, Teck-Peng Loh
Summary: This mini-review summarizes the recent progress in the direct synthesis of polyketide and polypeptide macrocycles using a transition-metal-catalyzed C-H bond activation strategy. It is divided into two parts: the first part describes the rhodium-catalyzed alkene-alkene ring-closing coupling for polyketide synthesis, and the second part summarizes the synthesis of polypeptide macrocycles. Additionally, transition-metal-catalyzed C-C bond macrocyclization reactions via alkyl C(sp(3))-H bond activation are also included. The authors hope that this mini-review will inspire researchers to explore new and broadly applicable strategies for C-C bond macrocyclization via intramolecular C-H activation.
Article
Agriculture, Multidisciplinary
Leng Wang, Ruiyuan Liu, Yue Meng, Fang Li, Huizhe Lu
Summary: The study highlights the crucial role of the C-terminus loop region in determining enzyme activity in IGPD. By conducting accelerated molecular dynamics simulations, the dynamics and functions of this region in Arabidopsis thaliana and Saccharomyces cerevisiae IGPD were investigated, providing insights for the design of selective inhibitors. Additionally, the study suggests IGPD as a potential antimicrobial target due to its absence in mammals.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Physical
J. Ree, Y. H. Kim, H. K. Shin
Summary: In this study, the redistribution of vibrational energy in the adenine-uracil base pair was investigated when interacting with a H2O molecule excited in an overtone-bending vibration. The energy transfer and redistribution processes were found to occur rapidly, with most intermolecular hydrogen bonds weakened but unbroken during the redistribution period. The efficiency of the energy redistribution was due to near-resonance between coupled vibrations.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Shin-ichi Muroi, Yoichiro Isohama
Summary: This study showed that the C-terminal domain of AQP5 is crucial for the localization of AQP5 on the plasma membrane, with Leu(262) identified as a key amino acid. Mutations in the C-terminal domain resulted in AQP5 being localized in the autophagosome or lysosome, leading to decreased protein stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Andrea Tura, Christian Goebl, Irfan Vardarli, Giovanni Pacini, Michael Nauck
Summary: This study aimed to explore the possible associations between incretins and insulin clearance in T2D patients. The results showed that there were no significant effects of incretins on insulin clearance, while different antidiabetic treatments may lead to variations in insulin clearance.
SCIENTIFIC REPORTS
(2022)
Review
Endocrinology & Metabolism
Ernesto Maddaloni, Geremia B. Bolli, Brian M. Frier, Randie R. Little, Richard D. Leslie, Paolo Pozzilli, Raffaela Buzzetti
Summary: C-peptide measurement is valuable in autoimmune diabetes but limited in type 2 diabetes due to confounding factors like insulin resistance. Standardization issues exist that may affect comparability between different laboratories.
DIABETES OBESITY & METABOLISM
(2022)
Article
Multidisciplinary Sciences
Itschak Axelrad, Mary Safrin, Rivka Cahan, Sang-Jin Suh, Dennis E. Ohman, Efrat Kessler
Summary: Pseudomonas aeruginosa secretes multiple endopeptidases, with LysC identified as the principal activating protease for pro-PaAP. Elastase and Apr indirectly participate in the activation of PaAP, and the N-terminal domain of AP56 may regulate hydrolysis of natural peptides/proteins.
Article
Biochemistry & Molecular Biology
Boning Cao, Zhongqiang Xia, Zhanzhang Hao, Changying Liu, Dingpei Long, Wei Fan, Aichun Zhao
Summary: The study investigated the function of MnNHX6, an endosomal-type NHX in mulberry, and found that the conserved region of its C-terminus plays a crucial role in protein function, stability, and interaction with other proteins. These findings contribute to a better understanding of the role of the C-terminal tail of NHXs in plants and the ion transport mechanism of NHX-like antiporters.
Article
Biochemistry & Molecular Biology
Xiaoqing Wang, Hao Liu, Yanfang Liu, Gefei Han, Yushu Wang, Haifeng Chen, Lin He, Gang Ma
Summary: The highly conserved hedgehog protein family plays important roles in embryonic development and tumorigenesis. This study focused on exploring the functions of the C-terminal region of the amino-terminal fragment of Indian Hedgehog (IHH-N), revealing its crucial role in signal transduction.
Article
Plant Sciences
Jacinto Gandullo, Rosario Alvarez, Ana-Belen Feria, Jose-Antonio Monreal, Isabel Diaz, Jean Vidal, Cristina Echevarria
Summary: A synthetic peptide, Glc-6P, and enzyme phosphorylation were found to regulate the proteolysis of C-4-PEPC. Specifically, two threonine residues in the peptide played an important role in this regulation process.
Article
Biochemistry & Molecular Biology
Savita Yadav, Ranajit Nivrutti Shinde, Suruchi Singh, Subramanian Karthikeyan, Balvinder Singh
Summary: The unstructured C-terminal residues of GCHII are crucial for its enzymatic activity, genetic deletion results in loss of enzyme activity, and His-190 may play an important role in the catalysis process.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biotechnology & Applied Microbiology
Sha Mei, Moran Li, Yiqi Sun, Xi Deng, Nifan Chen, Yang Liu, Jing Yin, Hongyi Luo, Yi Wu, Dan He, Fei Gan, Bing Tang, Xiao-Feng Tang
Summary: This study reveals the dependence of the proper maturation of Sec-dependent subtilase SptE in haloarchaea on the action of halolysin SptA. The study also highlights the importance of different extracellular and intracellular salt types as well as the long C-terminal extension in routing SptE through the Sec pathway. This research provides new insights into the secretion and adaptation mechanisms of Sec substrates in haloarchaea.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Siyu Chen, George Allen, Olesya O. Panasenko, Martine A. Collart
Summary: The Ccr4-Not complex plays multiple roles in mRNA life cycle. The Not4 subunit limits the solubility of MMF1 mRNA and regulates co-translation events by promoting ribosome pausing during MMF1 translation, which is crucial for its co-translational targeting to the mitochondria. This mechanism, called Mito-ENCay, involves various molecular components and processes, including mitochondrial outer membrane proteins, chaperones, and the Ccr4-Not complex.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Organic
Sudip Baguli, Abhishek Kundu, Soumajit Nath, Debashis Adhikari, Debabrata Mukherjee
Summary: Virtually irreversible intramolecular C-H activations are detrimental to aza-carbenes. A picolyl-tethered cyclic(alkyl)(amino)carbene (CAAC) undergoes isomerization into a donor-acceptor cyclopropane, but can be restored as a CAAC through retro-C-H activation in the presence of trapping agents such as Se or CuCl. The same donor-acceptor cyclopropane can be readily hydrolyzed to form a pyrrolidin-2-ol, acting as another precursor of picoCAAC, by undergoing 1,1-dehydration in the presence of Se or CuCl. This chemistry differs from the N-heterocyclic carbene analogue.
Article
Biology
Chao Wu, Zhewen Zhang, Chao Kang, Qiang Zhang, Weifeng Zhu, Yadong Zhang, Hao Zhang, Jingfa Xiao, Meilin Jin
Summary: This study evaluated the genomic differences between virulent and avirulent strains of Erysipelothrix rhusiopathiae to investigate the pathogenic mechanism. The results showed that a 120bp deletion in the avirulent strain's spaA gene affected its virulence and adhesion to porcine iliac artery endothelial cells, shedding light on the importance of C-terminal repeat units in SpaA-mediated adhesion.
Article
Biochemistry & Molecular Biology
Oanh Ho, Per Rogne, Tomas Edgren, Hans Wolf-Watz, Frederic H. Login, Magnus Wolf-Watz
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Cell Biology
Helene H. Jensen, Mikkel R. Holst, Frederic H. Login, Jeanette J. Morgen, Lene N. Nejsum
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Frederic H. Login, Helene H. Jensen, Gitte A. Pedersen, Manuel R. Amieva, Lene N. Nejsum
Article
Biochemistry & Molecular Biology
Frederic H. Login, Helene H. Jensen, Gitte A. Pedersen, Jennifer S. Koffman, Tae-Hwan Kwon, Maddy Parsons, Lene N. Nejsum
Review
Cell Biology
Sarannya Edamana, Frederic H. Login, Soichiro Yamada, Tae-Hwan Kwon, Lene N. Nejsum
Summary: Aquaporins (AQPs) are proteins that facilitate passive transport of water across cellular membranes, playing important roles in physiology such as regulation of body water homeostasis, skin hydration, and fat metabolism. Dysregulation of AQPs is associated with various pathophysiological conditions and major players in multiple cancers, influencing cellular signaling, migration, proliferation, and regulation of junctional proteins involved in cell-cell adhesion.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
H. Login, Johan Palmfeldt, Joleen S. Cheah, Soichiro Yamada, Lene N. Nejsum
Summary: The overexpression of AQP5 in cancers may contribute to carcinogenesis and cancer spread through two independent mechanisms: reduced cell-cell adhesion and increased cell migration capacity.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Mikkel R. Holst, Louis G. Jensen, Jesse Aaron, Frederic H. Login, Sampavi Rajkumar, Ute Hahn, Lene N. Nejsum
Summary: Regulated vesicle exocytosis, in response to extracellular stimuli, is crucial for processes like short-term urine concentration. In renal collecting ducts, AQP2-containing vesicles fuse with the plasma membrane upon stimulation by arginine vasopressin, enhancing water reabsorption. The 3D organization of these vesicles, which are similar in size to synaptic vesicles and associate with F-actin, was analyzed using 3D superresolution microscopy in a cell culture system.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Physiology
Christina Ernstsen, Frederic H. Login, Anne-Sofie B. Schelde, Jacob R. Therkildsen, Jakob Moller-Jensen, Rikke Norregaard, Helle Praetorius, Lene N. Nejsum
Summary: This study found that the urinary concentration defect in patients with acute pyelonephritis may be related to abnormal cellular localization of AQP2. Pathogenic bacteria can induce membrane targeting of AQP2, and this effect does not depend on an increase in cAMP levels or phosphorylation of the S256 site.
Review
Immunology
Anne Sofie Bruun-Sorensen, Sarannya Edamana, Frederic H. Login, Signe Borgquist, Lene N. Nejsum
Summary: Aquaporins, proteins that facilitate passive water transport across cellular membranes, are often overexpressed in various cancers, including pancreatic ductal adenocarcinoma. This overexpression of aquaporins, such as aquaporin-1, aquaporin-3, and aquaporin-5, has been linked to metastasis and poor survival in pancreatic cancer, suggesting their potential as diagnostic markers and therapeutic targets.
Review
Immunology
Malte Bystrup, Frederic H. Login, Sarannya Edamana, Signe Borgquist, Trine Tramm, Tae-Hwan Kwon, Lene N. Nejsum
Summary: Aquaporin-5 (AQP5) is essential in transepithelial water transport and its ectopic overexpression in breast cancer is associated with lymph node metastasis and poor prognosis. In addition to its role in water transport, AQP5 also plays a role in cancer metastasis, migration, and proliferation. Understanding the molecular mechanisms of AQP5 in cancer development and progression is crucial for its potential use as a prognostic biomarker and for the development of targeted interventions for breast cancer treatment.
Review
Immunology
Laura Traberg-Nyborg, Frederic H. Login, Sarannya Edamana, Trine Tramm, Signe Borgquist, Lene N. Nejsum
Summary: The overexpression of aquaporin in breast cancer is associated with poor prognosis and affects cellular processes like migration, angiogenesis, and proliferation, providing potential mechanisms for the progression and metastasis of breast cancer.
Article
Cell Biology
Sarannya Edamana, Frederic H. Login, Andreas Riishede, Vibeke S. Dam, Trine Tramm, Lene N. Nejsum
Summary: Breast carcinomas are associated with increased cell proliferation, migration, altered cellular adhesion, and loss of epithelial polarity. Aquaporin-5 (AQP5) is aberrantly expressed in breast cancer and is linked to metastasis and poor prognosis. AQP5 enhances cancer cell proliferation and migration, reduces cell-cell adhesion, and activates the Ras pathway. This study explores the relationship between AQP5 and the polarity protein Scribble. Results show that high AQP5 expression is inversely related to Scribble levels in breast carcinoma tissue samples. Overexpression of AQP5 in breast cancer cells reduces spheroid size, circularity, and Scribble levels. The effect of AQP5 on Scribble is partially dependent on AQP5-mediated activation of Ras. These findings suggest that AQP5 negatively regulates cellular polarity in breast cancer.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Stine Julie Tingskov, Mariagrazia D'Agostino, Frederic H. Login, Grazia Tamma, Lene N. Nejsum, Rikke Norregaard
Summary: In this study, the effect of tamoxifen (TAM) on the expression and localization of aquaporin 3 (AQP3) in collecting ducts was investigated. TAM attenuated the downregulation of AQP3 in a unilateral ureteral obstruction (UUO) model and a lithium-induced nephrogenic diabetes insipidus (NDI) model, and affected the intracellular localization in the collecting ducts. TAM also influenced the expression profile of other basolateral proteins.
Review
Urology & Nephrology
Frederic H. Login, Lene N. Nejsum
Summary: Aquaporins play a crucial role in renal water handling and body water homeostasis. They are also involved in water reabsorption and secretion in other tissues, transport of small molecules and gases, and various cellular processes. Furthermore, the expression of certain AQPs is related to cancer progression and drug response, suggesting their potential application in prognostics and therapeutics.
NATURE REVIEWS NEPHROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Helene H. Jensen, Frederic H. Login, Ji-Young Park, Tae-Hwan Kwon, Lene N. Nejsum
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2017)