Article
Biochemistry & Molecular Biology
Magdalena Zdrowowicz, Paulina Spisz, Aleksandra Hac, Anna Herman-Antosiewicz, Janusz Rak
Summary: This study found that breast and prostate cancer cells are more sensitive to the radiosensitizer 5-bromo-2'-deoxyuridine (BrdU) in a hypoxic environment. BrdU can more effectively induce double-strand breaks in cancer cells under hypoxia, thereby increasing sensitivity to radiotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Farhad Izadi, Adrian Szczyrba, Magdalena Datta, Olga Ciupak, Sebastian Demkowicz, Janusz Rak, Stephan Denifl
Summary: This study investigates the electron attachment reactions of BrSU and BrSdU molecules by using mass spectrometry and quantum chemical calculations. It is found that the yield of bromine anions is relatively low, possibly due to proton-transfer reactions in the transient negative ions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Catharina Erbacher, Nils Flothkoetter, Marcel Macke, C. Derrick Quarles, Michael Sperling, Jens Mueller, Uwe Karst
Summary: A fast and automated method for separation and quantification of bromide and 5-BrdU via IC and ICP-MS was developed, relevant for monitoring electron transfer efficiency along DNA. With a LOD of 30 ng/L, the method is suitable for screening applications with a multitude of samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Physical
Muhammad Saqib, Eugene Arthur-Baidoo, Farhad Izadi, Adrian Szczyrba, Magdalena Datta, Sebastian Demkowicz, Janusz Rak, Stephan Denifl
Summary: This study investigates the dissociative electron attachment of 5-iodo-4-thio-2'-deoxyuridine and demonstrates that low-energy electrons can effectively decompose the molecule, forming iodine anions and creating radical sites that may induce DNA strand breaks.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Hernan Mauricio Rivera, Esther Natalia Munoz, Daniel Osuna, Mauro Florez, Michael Carvajal, Luis Alberto Gomez
Summary: The study evaluated changes in microRNA expression and identified several miRNAs related to differential pigmentation and reduced proliferation in B16F1 melanoma cells exposed to 5-Brd-2'-dU and L-Tyr. The results suggested a coordinated and reciprocal co-expression of specific microRNAs with molecular targets involved in melanogenesis, cell cycle control, and senescence. These findings provide insights into the molecular basis of pigmentation changes and decreased proliferation in melanoma cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Daiki Nonoshita, Wataru Kashihara, Kazuhito Tanabe, Tasuku Isozaki, Yao Zhong Xu, Tadashi Suzuki
Summary: A novel thio-2'-deoxyuridine derivative with red-shifted absorption band and fluorescence properties was successfully synthesized, showing high quantum yield values under low oxygen concentrations. These findings are significant for the development of thio-2'-deoxyribonucleoside-based pharmaceuticals as DNA-specific photosensitizers for photochemotherapy.
JOURNAL OF PHYSICAL CHEMISTRY A
(2021)
Article
Chemistry, Multidisciplinary
Seung Woo Hong, Gon Ji Oh, Gil Tae Hwang
Summary: The study confirmed that introducing U-DAF derivative into the human telomeric iM sequence did not affect the formation and stability of the iM structure, with one of them potentially serving as an efficient fluorescent probe.
Article
Biochemistry & Molecular Biology
Maria Jose Pena-Gomez, Marina Suarez-Pizarro, Ivan V. Rosado
Summary: During DNA synthesis, the chemical modifications of cytosine 5hmC and 5hmU can cause genomic instability and replication fork impairment, especially in the absence of XRCC1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Joaquin Marti-Clua
Summary: Synthetic BrdU is a marker for DNA synthesis and has provided valuable insights into the cellular mechanisms of central nervous system development. However, the detrimental effects of BrdU incorporation into DNA on cell proliferation and viability are often overlooked. Studies focusing on prenatal and perinatal cerebellum development have important implications for understanding neuron generation, migration, and settlement patterns.
Article
Chemistry, Analytical
Mei Zhao, Guangrong Zou, Jing Tang, Jingyi Guo, Fang Wang, Zilin Chen
Summary: 5-carboxycytosine (5caC) is a crucial intermediate form in DNA methylation and demethylation processes, and its distribution and quantity have significant effects on organism's normal physiological activities. Due to its low abundance, detecting 5caC presents a challenge. In response, we propose a selective method for 5caC detection using differential pulse voltammetry (DPV) at a glassy carbon electrode (GCE) with probe labeling. This method allows quantitative detection of 5caC with good linearity and a low detection limit. It offers a promising alternative for 5caC detection in clinical samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Neurosciences
Lucia Rodriguez-Vazquez, Joaquin Marti
Summary: This study evaluated the effects of a single administration of BrdU on cell proliferation and apoptotic events in the prenatal cerebellum. Results showed that BrdU at various doses reduced mitotic figures and proliferating cells, with the most severe effects seen at higher doses. High doses of BrdU also led to the activation of apoptotic events, as shown by TUNEL assay and caspase-3 immunohistochemistry.
JOURNAL OF COMPARATIVE NEUROLOGY
(2021)
Article
Multidisciplinary Sciences
Juan de Dios Barba Tena, Etienne Schwob, Nicolas Talarek
Summary: This protocol describes a method using yeast cells and click reaction to visualize and quantify DNA replication, which is significant in studying cell cycle regulation and genome stability.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Chemistry, Inorganic & Nuclear
Ariadni Zianna, Ellie Vradi, Antonios G. Hatzidimitriou, Stavros Kalogiannis, George Psomas
Summary: Five neutral zinc(ii) complexes were synthesized and characterized, and the crystal structures of complexes 1 and 3 were determined by single-crystal X-ray crystallography. These compounds exhibit DNA intercalation, moderate DNA cleavage, reversible binding to serum albumins, and antioxidant and antimicrobial activities.
DALTON TRANSACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Sunbok Jang, Sripriya J. Raja, Vera Roginskaya, Matthew A. Schaich, Simon C. Watkins, Bennett Van Houten
Summary: UV-DDB has a non-canonical role in the processing of 8-oxoG and 5-hmdU. It stimulates the activities of OGG1, MUTYH, APE1, and SMUG1, and reduces the stability of SMUG1 on DNA. Furthermore, UV-DDB and SMUG1 interact transiently in cells.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Analytical
Jing Tang, Guangrong Zou, Chen Chen, Jing Ren, Fang Wang, Zilin Chen
Summary: This study introduced a novel electrochemical method for the selective detection of formylpyrimidines in DNA, specifically targeting 5-formyluracil (5fU) in the presence of 5-formylcytosine (5fC) and apyrimidinic (AP) sites. By combining molecule-depended chemical recognition and electrochemical sensing, the approach showed promise in sensitive and accurate detection of 5fU, with potential applications in various fields.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Anna Ligasova, Karel Koberna
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Anna Ligasova, Marketa Vydrzalova, Renata Burianova, Lenka Bruckova, Renata Vecerova, Anna Janost'akova, Karel Koberna
Article
Biochemistry & Molecular Biology
Anna Ligasova, Karel Koberna
Summary: This study developed a new concept of cytocentrifugation based on threaded connection, using a homemade device for the capture and processing of cell samples, demonstrating its efficiency for a variety of samples. This device not only offers a cost-effective alternative but also facilitates the eventual automation of the staining procedure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anna Ligasova, Karel Koberna
Summary: Cell quantification is widely used in both basic and applied research, with a special emphasis on techniques based on fluorescent DNA dyes. This review aims to guide readers through the various methods of cell quantification and highlight the strengths and weaknesses of these methods, particularly in terms of sensitivity, accuracy, and time. Additionally, some approaches for analyzing cell proliferation and viability are also described.
Review
Biochemistry & Molecular Biology
Anna Ligasova, Karel Koberna
Summary: Cell synchronization is commonly used for analyzing cellular metabolism at specific stages of the cell cycle, often achieved by inhibiting specific metabolic pathways. This review provides an overview of protocols for synchronizing mammalian cells based on inhibiting DNA synthesis, discussing their mechanisms, examples of use, and advantages and disadvantages to guide protocol selection for different research situations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anna Ligasova, Ivan Rosenberg, Marketa Bockova, Jiri Homola, Karel Koberna
Summary: This study introduces a novel approach for real-time measurement of uracil DNA glycosylase activity by immobilizing selected oligonucleotides on magnetic nanoparticles and utilizing Forster resonance energy transfer. The results demonstrate that this method provides more reliable data than using free oligonucleotides, and offers the possibility to investigate the relationship between uracil DNA glycosylase activity and the arrangement of oligonucleotide probes. Additionally, introducing a nick into oligonucleotides containing the target base (uracil) significantly decreases the activity of both bacterial glycosylase and glycosylases naturally present in nuclear lysates.
Article
Chemistry, Medicinal
Jiri Hodon, Ivo Frydrych, Zdenka Trhlikova, Jan Pokorny, Lucie Borkova, Sandra Benicka, Martin Vlk, Barbora Liskova, Agata Kubickova, Martina Medvedikova, Martin Pisar, Jan Sarek, Viswanath Das, Anna Ligasova, Karel Koberna, Petr Dzubak, Marian Hajduch, Milan Urban
Summary: A set of triterpenoid pyrazines and pyridines were prepared and tested for their cytotoxicity in cancer cell lines. The results showed that the triterpenoid core determined the activity of the compounds, while the heterocycle increased the activity and modulated the specificity. Several compounds showed high cytotoxicity against leukemia cells, especially in multidrug-resistant cells. Some compounds also inhibited the growth of solid tumors. These promising compounds have potential for further development as anticancer drugs.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Anna Ligasova, Ivo Frydrych, Karel Koberna
Summary: Cellular growth and preparation for division are collectively known as the cell cycle, which consists of several phases with different durations. The progression through these phases is regulated by both internal and external factors. Various methods, particularly those focusing on analyzing the duration of cell cycle phases, have been developed to study the role of these factors. This review aims to provide an overview of the basic methods for determining cell cycle phases and estimating their length, emphasizing on the effectiveness and reproducibility of these techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
