Editorial Material
Infectious Diseases
Hyesuk Seo, Weiping Zhang
LANCET INFECTIOUS DISEASES
(2020)
Article
Biotechnology & Applied Microbiology
Ti Lu, Rodney A. Moxley, Weiping Zhang
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Qiangde Duan, Wenwen Wu, Shengmei Pang, Zhiming Pan, Weiping Zhang, Guoqiang Zhu
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Review
Gastroenterology & Hepatology
Hyesuk Seo, Qiangde Duan, Weiping Zhang
Article
Biotechnology & Applied Microbiology
Eric Cox, Meryem Aloulou, James M. Fleckenstein, Christina Schaeffer, Asa Sjoling, Stephanie Schuller, Kurt Hanevik, Bert Devriendt, Weiping Zhang, Ann-Mari Svennerholm, Edward G. Dudley
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Immunology
Hyesuk Seo, Carolina Garcia, Xiaosai Ruan, Qiangde Duan, David A. Sack, Weiping Zhang
Summary: The lack of licensed vaccines for enterotoxigenic Escherichia coli (ETEC) poses a significant challenge, however, researchers have developed a novel epitope-based multiepitope fusion antigen (MEFA) vaccinology platform to construct two chimeric multivalent proteins that induced protective antibodies against key toxins and adhesins associated with ETEC. The resulting protein-based multivalent ETEC vaccine, MecVax, demonstrated broad immunogenicity and protection against ETEC diarrhea in preclinical models, highlighting its potential as an effective injectable vaccine for ETEC.
INFECTION AND IMMUNITY
(2021)
Article
Biotechnology & Applied Microbiology
Carolina Y. Garcia, Hyesuk Seo, David A. Sack, Weiping Zhang
Summary: This study demonstrates the broad immunogenicity of a multivalent ETEC subunit vaccine candidate and suggests that it can be effectively delivered via the intradermal route. The vaccine induces functional antibodies against ETEC adhesins and toxins when administered intradermally.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Richard M. Jones, Hyesuk Seo, Weiping Zhang, David A. Sack
Summary: The study developed a multi-epitope fusion antigen (MEFA) vaccine candidate and tested its protective effect against colonization of Enterotoxigenic E. coli (ETEC) using a rabbit model. The results showed that the vaccine generated antibodies against multiple colonization factors and prevented colonization of a challenge strain in the rabbit small intestine.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Hyesuk Seo, Qiangde Duan, Ipshita Upadhyay, Weiping Zhang
Summary: Enterotoxigenic Escherichia coli (ETEC) is a major cause of children's diarrhea and travelers' diarrhea, but there are currently no licensed vaccines against ETEC. Recent studies have shown that the protein-based vaccine candidate MecVax can generate functional antibodies against ETEC toxins and adhesins, providing protection in animal models. This study further confirms the broad immunogenicity of MecVax and evaluates the effect of vaccine antigen dose on antibody response and functional activities against ETEC toxins and bacterial adherence in mice, providing useful information for future human trials and the development of MecVax as an effective ETEC vaccine.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Siqi Li, Xinfeng Han, Ipshita Upadhyay, Weiping Zhang
Summary: This study identified functional epitopes of IpaB protein, particularly from the N terminus, and proposed using them for the development of a cross-protective Shigella vaccine. Three identified epitopes showed potential for inducing protective antibodies against Shigella invasion, indicating their usefulness in vaccine development.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Ipshita Upadhyay, Kathryn L. Lauder, Siqi Li, Galen Ptacek, Weiping Zhang
Summary: Currently, there is no licensed vaccine for enterotoxigenic Escherichia coli (ETEC), a major cause of diarrhea in developing countries and among travelers. A protein-based multivalent vaccine candidate called MecVax has been shown to induce antibodies that neutralize ETEC toxins and inhibit bacterial adherence. In this study, adult rabbits immunized with MecVax showed a significant reduction in intestinal colonization by ETEC, suggesting that MecVax may be an effective vaccine candidate against ETEC.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Kyle Brumfield, Hyesuk Seo, Nnebuefe Idegwu, Chad Artman, Laura Gonyar, James Nataro, Weiping Zhang, David Sack, James Geyer, Julius Goepp
Summary: This research evaluates the feasibility of using avian immunoglobulins (IgY) to prevent diarrheal illness caused by enterotoxigenic Escherichia coli (ETEC). The results show that IgY can inhibit the adhesion of ETEC and has the potential to be a prophylactic treatment for diarrheal diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ipshita Upadhyay, Siqi Li, Galen Ptacek, Hyesuk Seo, David A. Sack, Weiping Zhang
Summary: By using a multiepitope fusion antigen vaccinology platform, researchers constructed a polyvalent protein immunogen that can induce immune response and cross-protection. The immunogen was shown to generate antibodies against various virulence factors of Vibrio cholerae and provide protection against bacterial colonization and related diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Ipshita Upadhyay, Shafiullah M. D. Parvej, Siqi Li, Kathryn L. Lauder, Yiyang Shen, Weiping Zhang
Summary: An effective vaccine against ETEC is lacking, which is a primary cause of children's and traveler's diarrhea and a global health threat. The key challenge in ETEC vaccine development is the expression of heterogeneous virulence determinants by ETEC bacteria. This study utilized a unique vaccinology platform to develop a polyvalent antigen that demonstrated broad immunogenicity and functions against targeted ETEC strains.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Qiangde Duan, Shengmei Pang, Wenwen Wu, Boyu Jiang, Weiping Zhang, Siguo Liu, Xiaojun Wang, Zhiming Pan, Guoqiang Zhu
VETERINARY RESEARCH
(2020)