Article
Biochemistry & Molecular Biology
Kamiya Bridges, Humphrey H-C Yao, Barbara Nicol
Summary: This study reveals the significance of the Runx1 gene in mouse ovarian development. Loss of Runx1 leads to an increased prevalence of ovarian tumors and also affects normal granulosa cell differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Karina F. Rodriguez, Paula R. Brown, Ciro M. Amato, Barbara Nicol, Chia-Feng Liu, Xin Xu, Humphrey Hung-Chang Yao
Summary: In most animals, the fate determination and maintenance of fetal testes occur cell autonomously. In XY mouse embryos, Sertoli cell-derived anti-Mullerian hormone (AMH) and activin B together maintain the identity of Sertoli cells, and the absence of these factors leads to transdifferentiation into female granulosa cells, resulting in ovotestis formation.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Maya R. Overland, Yi Li, Amber Derpinghaus, Sena Aksel, Mei Cao, Nicholas Ladwig, Gerald R. Cunha, Marta Himelreich-Peric, Laurence S. Baskin
Summary: Accurate diagnosis of the presence or absence of functional ovarian tissue in clinical specimens relies on a clear definition of normal human fetal and early postnatal ovarian development. By studying human ovarian specimens from 8 weeks of gestation through 16 years of postnatal, we have identified the protein expression patterns and stages of follicular development in the early fetal through post-pubertal human ovary. However, more research is needed to improve the identification of ovarian stromal components in patients with disorders of sexual differentiation.
Article
Biochemistry & Molecular Biology
Maya Barsky, Jamie Merkison, Pardis Hosseinzadeh, Liubin Yang, Janet Bruno-Gaston, Jay Dunn, William Gibbons, Chellakkan Selvanesan Blesson
Summary: Polycystic ovary syndrome (PCOS) is a common form of anovulatory infertility with a strong hereditary component. Research indicates that excessive prenatal androgens can affect ovarian development by altering gene expression and mitochondrial function. This programming may begin at birth and is influenced by androgens in utero.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tomoka Misawa, Kazuhiro Hitomi, Kenichi Miyata, Yoko Tanaka, Risa Fujii, Masatomo Chiba, Tze Mun Loo, Aki Hanyu, Hiroko Kawasaki, Hisaya Kato, Yoshiro Maezawa, Koutaro Yokote, Asako J. Nakamura, Koji Ueda, Nobuo Yaegashi, Akiko Takahashi
Summary: Senescent cells exhibit a secretory phenotype that promotes inflammation and age-related diseases. This study identified ATP6V0D1 and RTN4 as novel markers upregulated in small extracellular vesicles (sEVs) from senescent cells and aged mice, suggesting their potential use for detecting senescent cells in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Gun Srijuntongsiri, Atiwat Mhoowai, Sukuma Samngamnim, Pornchalit Assavacheep, Janine T. Bosse, Paul R. Langford, Navaporn Posayapisit, Ubolsree Leartsakulpanich, Warangkhana Songsungthong
Summary: In this study, 7 novel A. pleuropneumoniae-specific DNA markers were identified through genome mining and molecular methods. These markers can serve as additional or alternative targets for disease diagnostics, potentially improving disease control.
MICROBIOLOGY SPECTRUM
(2022)
Article
Genetics & Heredity
Rafiatu Azumah, Katja Hummitzsch, Monica D. Hartanti, Justin C. St. John, Richard A. Anderson, Raymond J. Rodgers
Summary: This study analyzed the expression of polycystic ovary syndrome (PCOS) candidate genes in bovine fetal ovaries and identified different expression patterns and upstream regulators associated with these genes. The early cluster of genes was mainly involved in mitochondrial function and oxidative phosphorylation, while the late cluster of genes was associated with stromal expansion, cholesterol biosynthesis, and steroidogenesis. These findings provide insights into the development and origins of PCOS, suggesting that multiple etiological pathways may contribute to the development of the syndrome.
FRONTIERS IN GENETICS
(2022)
Article
Physiology
Amir Hossan Shaikat, Masami Ochiai, Akari Sasaki, Misa Takeda, Akari Arima, Takeshi Ohkubo
Summary: This study demonstrates that leptin modulates ovarian development and folliculogenesis marker genes primarily by acting on ovaries on the specific ovarian-remodeling days in post-hatch chicks, which may alter folliculogenesis and ovarian development toward puberty in chicken.
FRONTIERS IN PHYSIOLOGY
(2021)
Editorial Material
Oncology
Ana C. Veneziani, Amit M. Oza
Summary: Targeted therapy for low-grade serous ovarian carcinomas is still an unmet need, but recent advances in molecular characterization have widened the scope for novel targeted therapies.
CLINICAL CANCER RESEARCH
(2022)
Article
Horticulture
Sikandar Amanullah, Peng Gao, Benjamin Agyei Osae, Arvind Saroj, Tiantian Yang, Shi Liu, Yiqun Weng, Feishi Luan
Summary: This study utilized whole genome resequencing and SNP markers to detect genetic linkage mapping and QTLs for ovary, fruit, and seed traits in melon. A total of 26 putative QTLs were identified across different chromosomes, with significant relationships and normal distribution frequencies observed in phenotype analysis. Further exploration and fine mapping of these QTLs could prove valuable in unexplored botanical groups of melon.
SCIENTIA HORTICULTURAE
(2021)
Article
Cell Biology
Franz Markert, Alexander Storch
Summary: Oxygen tension affects cortical development, and hyperoxygenation during mid-neurogenesis phase accelerates cortical development and increases the number of cortical layer 5 neurons until birth.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Ying Fu, Annaliese S. Mason, Yaofeng Zhang, Huasheng Yu
Summary: The FAD2 genes play a crucial role in determining the oleic acid content in rapeseed oil, with different alleles leading to variations in oil composition. Through QTL mapping and SNP markers, four QTL related to C18:1 content were identified, with BnFAD2.A05 and BnFAD2.C05 genes explaining a significant portion of the variation. Novel mutant alleles were found near specific protein motifs, resulting in higher oleic acid content in seed oil when combined. Competitive allele-specific PCR markers based on these mutations were successfully developed and validated, aiding in breeding for ultra-high oleic acid content in oilseed rape.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Oncology
Ha-Yeon Shin, Eun-ju Lee, Wookyeom Yang, Hyo Sun Kim, Dawn Chung, Hanbyoul Cho, Jae-Hoon Kim
Summary: This study investigates the clinical application and gene expression profiling of gynecologic cancer PDX models. The results show that the success rate of PDX is correlated with patient prognosis, and ovarian cancer patients with a poor prognosis exhibit faster PDX growth rate.
Article
Biochemistry & Molecular Biology
Huimin Niu, Xuetong An, Xinpei Wang, Min Yang, Fei Cheng, Anmin Lei, Jun Luo
Summary: The fatty acid metabolism network plays a vital role in providing energy for oocyte growth and follicle formation during early mouse oogenesis. However, the mechanism behind this process remains unknown. By analyzing the gene expression in perinatal ovaries of gene-edited mice lacking the Scd1 gene, we found that Scd1 deficiency disrupts the expression of meiosis-related genes and various genes associated with oocyte growth and differentiation, leading to a lower oocyte maturation rate. Furthermore, Scd1 absence also affects fatty acid metabolism genes and lipid droplet content. Overall, our findings highlight the significant role of Scd1 in regulating fatty acid networks essential for oocyte maintenance and differentiation during early follicular genesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Endocrinology & Metabolism
Laetitia L. Lecante, Sabrina Leverrier-Penna, Thomas Gicquel, Frank Giton, Nathalie Costet, Christele Desdoits-Lethimonier, Laurianne Lesne, Bernard Fromenty, Vincent Lavoue, Antoine D. Rolland, Severine Mazaud-Guittot
Summary: The study found that APAP can impact the development of fetal human ovaries, especially during the 10-12 week window of heightened sensitivity. APAP reduced the total cell number in ovaries, induced cell death, and decreased KI67-positive cell density independently of fetal age.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Letter
Pathology
Stefanie Aranza, Christina Roydhouse, Catherine Mitchell, Joseph H. A. Vissers, Wing-Yee Lo, Sean M. Grimmond, Jane Kho
Letter
Pathology
C. Mitchell, V. Malalasekera, A. J. Gill, J. H. A. Vissers, S. J. Luen, S. M. Grimmond, J. Lewin
Letter
Hematology
Georgina L. Ryland, Masayuki Umeda, Linda Holmfeldt, Soren Lehmann, Morten Krogh Herlin, Jing Ma, Mahsa Khanlari, Jeffrey E. Rubnitz, Rhonda E. Ries, Hansen J. Kosasih, Paul G. Ekert, Hwee Ngee Goh, Ing S. Tiong, Sean M. Grimmond, Claudia Haferlach, Ryan B. Day, Timothy J. Ley, Soheil Meshinchi, Xiaotu Ma, Piers Blombery, Jeffery M. Klco
Article
Developmental Biology
Guillaume Burnet, Chun-Wei Allen Feng, Ka Man Fiona Cheung, Josephine Bowles, Cassy M. Spiller
Summary: This study describes the generation of the Ddx4-P2A-iCre allele through CRISPR/Cas9 targeted insertion of an improved Cre sequence. Functional assessment of this allele indicates that Cre activity begins in primordial germ cells from at least E10.5 and achieves higher efficiency for early gonadal deletion compared to the inducible Oct4(CreERT2) line. The Ddx4(iCreJoBo) allele shows hypomorphic Ddx4 expression and infertility in homozygous males, with ectopic activity observed in multiple organs.
Correction
Multidisciplinary Sciences
Ludmil B. Alexandrov, Jaegil Kim, Nicholas J. Haradhvala, Mi Ni Huang, Alvin Wei Tian Ng, Yang Wu, Arnoud Boot, Kyle R. Covington, Dmitry A. Gordenin, Erik N. Bergstrom, S. M. Ashiqul Islam, Nuria Lopez-Bigas, Leszek J. Klimczak, John R. McPherson, Sandro Morganella, Radhakrishnan Sabarinathan, David A. Wheeler, Ville Mustonen, Gad Getz, Steven G. Rozen, Michael R. Stratton
Correction
Multidisciplinary Sciences
Yilong Li, Nicola D. Roberts, Jeremiah A. Wala, Ofer Shapira, Steven E. Schumacher, Kiran Kumar, Ekta Khurana, Sebastian Waszak, Jan O. Korbel, James E. Haber, Marcin Imielinski, Joachim Weischenfeldt, Rameen Beroukhim, Peter J. Campbell
Correction
Multidisciplinary Sciences
Moritz Gerstung, Clemency Jolly, Ignaty Leshchiner, Stefan C. Dentro, Santiago Gonzalez, Daniel Rosebrock, Thomas J. Mitchell, Yulia Rubanova, Pavana Anur, Kaixian Yu, Maxime Tarabichi, Amit Deshwar, Jeff Wintersinger, Kortine Kleinheinz, Ignacio Vazquez-Garcia, Kerstin Haase, Lara Jerman, Subhajit Sengupta, Geoff Macintyre, Salem Malikic, Nilgun Donmez, Dimitri G. Livitz, Marek Cmero, Jonas Demeulemeester, Steven Schumacher, Yu Fan, Xiaotong Yao, Juhee Lee, Matthias Schlesner, Paul C. Boutros, David D. Bowtell, Hongtu Zhu, Gad Getz, Marcin Imielinski, Rameen Beroukhim, S. Cenk Sahinalp, Yuan Ji, Martin Peifer, Florian Markowetz, Ville Mustonen, Ke Yuan, Wenyi Wang, Quaid D. Morris, Paul T. Spellman, David C. Wedge, Peter Van Loo
Review
Cell Biology
John S. S. Mattick, Paulo P. P. Amaral, Piero Carninci, Susan Carpenter, Howard Y. Y. Chang, Ling-Ling Chen, Runsheng Chen, Caroline Dean, Marcel E. E. Dinger, Katherine A. A. Fitzgerald, Thomas R. R. Gingeras, Mitchell Guttman, Tetsuro Hirose, Maite Huarte, Rory Johnson, Chandrasekhar Kanduri, Philipp Kapranov, Jeanne B. B. Lawrence, Jeannie T. T. Lee, Joshua T. T. Mendell, Timothy R. R. Mercer, Kathryn J. J. Moore, Shinichi Nakagawa, John L. L. Rinn, David L. L. Spector, Igor Ulitsky, Yue Wan, Jeremy E. E. Wilusz, Mian Wu
Summary: Genes encoding lncRNAs are abundant in complex organisms and are transcribed by RNA polymerase I, II, and III, as well as processed introns. The classification and annotation of lncRNAs are challenging due to their various functions, isoforms, and interactions with other genes. These lncRNAs evolve rapidly, are cell type-specific, and regulate multiple cellular processes, including gene expression and translation control.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Genetics & Heredity
Zornitza Stark, Tiffany Boughtwood, Matilda Haas, Jeffrey Braithwaite, Clara L. Gaff, Ilias Goranitis, Amanda B. Spurdle, David P. Hansen, Oliver Hofmann, Nigel Laing, Sylvia Metcalfe, Ainsley J. Newson, Hamish S. Scott, Natalie Thorne, Robyn L. Ward, Marcel E. Dinger, Stephanie Best, Janet C. Long, Sean M. Grimmond, John Pearson, Nicola Waddell, Christopher P. Barnett, Matthew Cook, Michael Field, David Fielding, Stephen B. Fox, Jozef Gecz, Adam Jaffe, Richard J. Leventer, Paul J. Lockhart, Sebastian Lunke, Andrew J. Mallett, Julie McGaughran, Linda Mileshkin, Katia Nones, Tony Roscioli, Ingrid E. Scheffer, Christopher Semsarian, Cas Simons, David M. Thomas, David R. Thorburn, Richard Tothill, Deborah White, Sally Dunwoodie, Peter T. Simpson, Peta Phillips, Marie-Jo Brion, Keri Finlay, Michael CJ. Quinn, Tessa Mattiske, Emma Tudini, Kirsten Boggs, Sean Murray, Kathy Wells, John Cannings, Andrew H. Sinclair, John Christodoulou, Kathryn N. North
Summary: Australian Genomics is a national collaborative partnership that aims to integrate genomics into healthcare through a whole-of-system approach. It has evaluated genomic testing outcomes in over 5,200 individuals across rare disease and cancer studies and provided evidence-based changes in policy and practice, resulting in government funding and access to genomic tests. It has also developed national skills, infrastructure, policy, and data resources to support data sharing and improve clinical genomic delivery.
AMERICAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Ronnie Ren Jie Low, Ka Yee Fung, Hugh Gao, Adele Preaudet, Laura F. Dagley, Jumana Yousef, Belinda Lee, Samantha J. Emery-Corbin, Rune H. Larsen, Nadia J. Kershaw, Antony W. Burgess, Peter Gibbs, Frederic Hollande, Michael D. W. Griffin, Sean M. Grimmond, Tracy L. Putoczki
Summary: Epithelial-mesenchymal transition (EMT) is a continuum associated with cancer progression, invasive capabilities, and metastasis. Through a sporadic model of pancreatic cancer, a murine organoid biobank was generated to study the different EMT states. It was found that organoid morphology predicts the EMT state, and that TGF beta 1 induces changes in the S100 family, complete EMT, and high-grade tumors. S100A4 may serve as a biomarker for predicting EMT state, disease progression, and outcome in pancreatic cancer patients.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Oncology
Genevieve Dall, Cassandra J. J. Vandenberg, Ksenija Nesic, Gayanie Ratnayake, Wenying Zhu, Joseph H. A. Vissers, Justin Bedo, Jocelyn Penington, Matthew J. J. Wakefield, Damien Kee, Amandine Carmagnac, Ratana Lim, Kristy Shield-Artin, Briony Milesi, Amanda Lobley, Elizabeth L. L. Kyran, Emily O'Grady, Joshua Tram, Warren Zhou, Devindee Nugawela, Kym Pham Stewart, Reece Caldwell, Lia Papadopoulos, Ashley P. P. Ng, Alexander Dobrovic, Stephen B. B. Fox, Orla McNally, Jeremy D. D. Power, Tarek Meniawy, Teng Han Tan, Ian M. M. Collins, Oliver Klein, Stephen Barnett, Inger Olesen, Anne Hamilton, Oliver Hofmann, Sean Grimmond, Anthony T. T. Papenfuss, Clare L. L. Scott, Holly E. E. Barker
Summary: This study identified mutations in DNA repair genes in a subset of uterine leiomyosarcoma patients and found that these patients may benefit from PARP inhibitor therapy. Patient-derived xenograft models confirmed the efficacy of this treatment approach.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Oncology
Jessica L. Chitty, Michelle Yam, Lara Perryman, Amelia L. Parker, Joanna N. Skhinas, Yordanos F. I. Setargew, Ellie T. Y. Mok, Emmi Tran, Rhiannon D. Grant, Sharissa L. Latham, Brooke A. Pereira, Shona C. Ritchie, Kendelle J. Murphy, Michael Trpceski, Alison D. Findlay, Pauline Melenec, Elysse C. Filipe, Audrey Nadalini, Sipiththa Velayuthar, Gretel Major, Kaitlin Wyllie, Michael Papanicolaou, Shivanjali Ratnaseelan, Phoebe A. Phillips, George Sharbeen, Janet Youkhana, Alice Russo, Antonia Blackwell, Jordan F. Hastings, Morghan C. Lucas, Cecilia R. Chambers, Daniel A. Reed, Janett Stoehr, Claire Vennin, Ruth Pidsley, Anaiis Zaratzian, Andrew M. Da Silva, Michael Tayao, Brett Charlton, David Herrmann, Max Nobis, Susan J. Clark, Andrew V. Biankin, Amber L. Johns, David R. Croucher, Adnan Nagrial, Anthony J. Gill, Sean M. Grimmond, Marina Pajic, Paul Timpson, Wolfgang Jarolimek, Thomas R. Cox
Summary: By inhibiting the activity of pan-lysyl oxidase, PXS-5505 can reduce fibrosis and stiffness in pancreatic tumors, decrease cancer cell invasion and metastasis, and enhance the efficacy of chemotherapy.
Review
Oncology
Kevin Tran, Owen W. J. Prall, Catherine Mitchell, Angela Chou, Anthony J. Gill, Sean M. Grimmond, Grace Kong, Gareth Kiernan, Cristina Torche, Lara Lipton, Benjamin Thomson, H. S. Ko
Summary: Pancreatic acinar cell carcinoma (ACC) is a rare pancreatic neoplasm that is often difficult to diagnose due to lack of awareness and knowledge about its clinical, radiological, and pathological characteristics. Accurate diagnosis is crucial as it can be easily mistaken for pancreatic ductal adenocarcinoma (PDAC) or pancreatic neuroendocrine tumor (PNET). This case study highlights the importance of integrating clinical, imaging, molecular histopathological, and genomic analysis for the diagnosis and treatment of complex pancreatic ACC.
CURRENT PROBLEMS IN CANCER: CASE REPORTS
(2023)
Article
Endocrinology & Metabolism
Anthony D. Bird, Emily R. Frost, Stefan Bagheri-Fam, Brittany M. Croft, Janelle M. Ryan, Liang Zhao, Peter Koopman, Vincent R. Harley
Summary: During sex determination in mice, FGFR2c is involved in testis development while FOXL2 and WNT4/RSPO1 pathways drive ovarian development. The role of FGFR2 in the ovary and its requirement in the testis after sex determination are not clear.
Article
Biochemical Research Methods
Mahdi Zeraati, Samuel E. Ross, Behnaz Aghaei, Alvaro Gonzalez Rajal, Cecile King, Marcel E. Dinger
Summary: Intercalated motifs or i-Motifs (iMs) are nucleic acid structures formed by cytosine-rich sequences, which can regulate cellular processes and have broad applications in nanotechnology due to their pH-dependent nature. We have developed an iM-specific nanobody (iMbody) that can recognize iM DNA structures regardless of their sequences, making it a versatile research tool. In this article, we provide a protocol for the bacterial expression and purification of iMbody, as well as procedures for performing ELISA and immunostaining using iMbody.
