Article
Multidisciplinary Sciences
Jingru Wang, Na Fang, Juan Xiong, Yuanjiao Du, Yue Cao, Wei-Ke Ji
Summary: Upon starvation, cells switch to mitochondrial oxidation of fatty acids from glucose-based metabolism. VPS13D and ESCRT complex remodel lipid droplet (LD) membranes to promote fatty acid trafficking to mitochondria.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Shaun K. Bremner, Rebecca Berends, Alexandra Kaupisch, Jennifer Roccisana, Calum Sutherland, Nia J. Bryant, Gwyn W. Gould
Summary: Insulin stimulates glucose transport by regulating the delivery of GLUT4-containing vesicles. Sx16 and mVps45 have been identified as key proteins involved in the delivery of GLUT4. Mutations in the binding sites of these proteins affect GLUT4 sorting, and phosphorylation of Sx16 at T7 is important for GLUT4 trafficking.
Article
Biology
Junko Y. Toshima, Ayana Tsukahara, Makoto Nagano, Takuro Tojima, Daria E. Siekhaus, Akihiko Nakano, Jiro Toshima, Sean Munro
Summary: Budding yeast possesses an independent endocytic early/sorting compartment within the trans-Golgi network (TGN) that sorts endocytic cargos. This compartment functions as the target for endocytic vesicles and is involved in recycling to the plasma membrane or transport to the Rab5 endosomal compartment. The Tlg2p protein, a homolog of yeast syntaxin, resides in this compartment and acts as a Q-SNARE for endocytic vesicles.
Article
Biochemistry & Molecular Biology
Francisco Diaz-Saez, Carla Blanco-Sinfreu, Adria Archilla-Ortega, David Sebastian, Montserrat Romero, Maria Isabel Hernandez-Alvarez, Silvia Mora, Xavier Testar, Wifredo Ricart, Jose Manuel Fernandez-Real, Jose Maria Moreno-Navarrete, Julian Aragones, Marta Camps, Antonio Zorzano, Anna Guma
Summary: The downregulation of Nrg4 in adipocytes impairs insulin action, likely due to decreased insulin receptor and Glut4 proteins, while also increasing proinflammatory cytokine expression and upregulating proteins involved in autophagy. Restoring lysosomal activity can partially reverse these effects, indicating that Nrg4 plays a role in maintaining insulin responsiveness and regulating autophagy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Pierre-Jean Cornejo, Bastien Vergoni, Mickael Ohanna, Brice Angot, Teresa Gonzalez, Jennifer Jager, Jean-Francois Tanti, Mireille Cormont
Summary: This study identifies miR-34a as a new mediator of adipocyte insulin resistance. Upregulation of miR-34a inhibits insulin signaling and decreases glucose transport, accompanied by a reduced expression of VAMP2 and an increased expression of PTP1B. Mechanistically, miR-34a decreases NAD(+) levels, thereby inhibiting Sirtuin-1 and promoting the upregulation of PTP1B. The mRNA expression of Nampt and Naprt is also decreased in adipose tissue of obese mice.
Article
Cell Biology
Sebastian Baenfer, Sophie Kutscher, Fenja Fleck, Martina Dienst, Christian Preusser, Elke Pogge von Strandmann, Ralf Jacob
Summary: In this study, we demonstrate that E-cadherin can be recruited into the membrane of extracellular vesicles through a specific domain in its cytoplasmic tail that interacts with Tsg101. This finding contributes to a better understanding of the formation and function of extracellular vesicles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Yan Zhang, Jing Cui, Ze Wang, Ying Zhu, Ningkun Zhang, Yu Chen
Summary: This study shows that Apelin stimulates GLUT4 translocation and improves glucose utilization through the AKt and eNOS pathways. Apelin can potentially enhance the mechanism of insulin resistance by mediating the inflammatory response in 3T3-L1 adipocytes.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Cell Biology
Chao Wei, Lumeng Jia, Xiaona Huang, Jin Tan, Mulan Wang, Jing Niu, Yingping Hou, Jun Sun, Pengguihang Zeng, Jia Wang, Li Qing, Lin Ma, Xinyi Liu, Xiuxiao Tang, Fenjie Li, Shaoshuai Jiang, Jingxin Liu, Tingting Li, Lili Fan, Yujie Sun, Juntao Gao, Cheng Li, Junjun Ding
Summary: This study reveals a novel role of CTCF in organizing long-range chromatin interactions, where CTCF phase separation mediated by RYBP promotes inter-A compartment interactions and enhances the self-renewal ability of embryonic stem cells.
Article
Cell Biology
Yenniffer Avalos, Maria Paz Hernandez-Caceres, Pablo Lagos, Daniela Pinto-Nunez, Patricia Rivera, Paulina Burgos, Francisco Diaz-Castro, Michelle Joy-Immediato, Leslye Venegas-Zamora, Erik Lopez-Gallardo, Catalina Kretschmar, Ana Batista-Gonzalez, Flavia Cifuentes-Araneda, Lilian Toledo-Valenzuela, Marcelo Rodriguez-Pena, Jasson Espinoza-Caicedo, Claudio Perez-Leighton, Cristina Bertocchi, Mauricio Cerda, Rodrigo Troncoso, Valentina Parra, Mauricio Budini, Patricia Burgos, Alfredo Criollo, Eugenia Morselli
Summary: Palmitic acid leads to the loss of primary cilia in the hypothalamus, impairing insulin signaling and insulin sensitivity.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Sung Ho Ahn, Hyun Jung Lee, Do Hyeon Pyun, Tae Jin Kim, A. M. Abd El-Aty, Jin-Ho Song, Yong Kyoo Shin, Ji Hoon Jeong, Eon Sub Park, Tae Woo Jung
Summary: CAP can attenuate lipogenesis and enhance lipolysis in adipocytes through the irisin/AMPK pathway, suggesting that it may be a novel therapeutic agent for obesity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Virology
Wenyuan Shen, Chang Liu, Yue Hu, Qian Ding, Jiabin Feng, Zhou Liu, Xiaohong Kong
Summary: This study found that the replication of human immunodeficiency virus-1 (HIV-1) is inhibited when the level of spastin protein in cells is reduced, because spastin enhances virus production by promoting the lysosomal degradation of the Gag protein. Further investigation revealed that increased sodium tolerance 1 (IST1), a subunit of the endosomal sorting complex required for transport (ESCRT), can interact with the MIT domain of spastin to regulate the production of intracellular Gag. In summary, spastin is crucial for HIV-1 replication, and the interaction between spastin and IST1 facilitates virus production by regulating the intracellular trafficking and degradation of Gag protein. Therefore, spastin may serve as a new target for HIV-1 prophylactic and therapy.
Article
Pharmacology & Pharmacy
Bhavimani Guru, Akhilesh K. Tamrakar, Subhankar P. Mandal, Prashantha B. R. Kumar, Aditya Sharma, Santhepete Nanjundaiah Manjula
Summary: The study designed and synthesized a novel partial PPAR gamma agonist GS2, which showed weaker lipogenic effect in cell experiments and enhanced GLUT4 translocation stimulatory effect in skeletal muscle cells. GS2 may serve as a promising therapeutic agent for the treatment of insulin resistance and type 2 diabetes mellitus without adiposity.
Article
Endocrinology & Metabolism
Yosuke Masubuchi, Jinhui Ma, Tomohiro Suzuki, Itaru Kojima, Takeshi Inagaki, Hiroshi Shibata
Summary: T1R3 receptor plays a non-gustatory role in adipocytes by negatively regulating insulin action of glucose transport through G alpha s-dependent microtubule disassembly.
Article
Cardiac & Cardiovascular Systems
Jing Tian, Yi-Yue Zhang, Ya-Wei Peng, Bin Liu, Xiao-Jie Zhang, Zhong-Yang Hu, Chang-Ping Hu, Xiu-Ju Luo, Jun Peng
Summary: Polymyxin B can reduce brain injury and necroptosis in stroke rats by enhancing the ESCRT-III machinery and suppressing the RIPK1/RIPK3/MLKL pathway simultaneously.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2022)
Article
Cell Biology
Julia D. Romano, Joshua Mayoral, Rebekah B. Guevara, Yolanda Rivera-Cuevas, Vern B. Carruthers, Louis M. Weiss, Isabelle Coppens
Summary: Intracellular pathogens, such as Toxoplasma gondii, manipulate host cells to exploit cellular resources. The parasite sequesters host nutrient-filled organelles into its parasitophorous vacuole (PV) through deep invaginations of the PV membrane (PVM). The parasite utilizes the host ESCRT-III and Vps4A to create PVM buds and vesicles.
JOURNAL OF CELL SCIENCE
(2023)
Article
Chemistry, Medicinal
Amit Nathubhai, Teemu Haikarainen, Jarkko Koivunen, Sudarshan Murthy, Francoise Koumanov, Matthew D. Lloyd, Geoffrey D. Holman, Taina Pihlajaniemi, David Tosh, Lari Lehtio, Michael D. Threadgill
JOURNAL OF MEDICINAL CHEMISTRY
(2017)
Article
Neurosciences
Javier T. Gonzalez, Judith D. Richardson, Enhad A. Chowdhury, Francoise Koumanov, Geoffrey D. Holman, Scott Cooper, Dylan Thompson, Kostas Tsintzas, James A. Betts
JOURNAL OF PHYSIOLOGY-LONDON
(2018)
Article
Endocrinology & Metabolism
Robert M. Edinburgh, Aaron Hengist, Harry A. Smith, Rebecca L. Travers, Francoise Koumanov, James A. Betts, Dylan Thompson, Jean-Philippe Walhin, Gareth A. Wallis, D. Lee Hamilton, Emma J. Stevenson, Kevin D. Tipton, Javier T. Gonzalez
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2018)
Article
Multidisciplinary Sciences
Yun-Chu Chen, Tristan Smith, Robert H. Hicks, Aswin Doekhie, Francoise Koumanov, Stephen A. Wells, Karen J. Edler, Jean van den Elsen, Geoffrey D. Holman, Kevin J. Marchbank, Asel Sartbaeva
SCIENTIFIC REPORTS
(2017)
Review
Biochemistry & Molecular Biology
Geoffrey D. Holman
BIOCHEMICAL JOURNAL
(2018)
Article
Multidisciplinary Sciences
Manuel Bano-Polo, Carlos Baeza-Delgado, Silvia Tamborero, Anthony Hazel, Brayan Grau, IngMarie Nilsson, Paul Whitley, James C. Gumbart, Gunnar von Heijne, Ismael Mingarro
NATURE COMMUNICATIONS
(2018)
Review
Neurosciences
Aaron Hengist, Francoise Koumanov, Javier T. Gonzalez
JOURNAL OF PHYSIOLOGY-LONDON
(2019)
Article
Endocrinology & Metabolism
Robert M. Edinburgh, Helen E. Bradley, Nurul-Fadhilah Abdullah, Scott L. Robinson, Oliver J. Chrzanowski-Smith, Jean-Philippe Walhin, Sophie Joanisse, Konstantinos N. Manolopoulos, Andrew Philp, Aaron Hengist, Adrian Chabowski, Frances M. Brodsky, Francoise Koumanov, James A. Betts, Dylan Thompson, Gareth A. Wallis, Javier T. Gonzalez
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2020)
Review
Physiology
Geoffrey D. Holman
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Paul Whitley, Brayan Grau, James C. Gumbart, Luis Martinez-Gil, Ismael Mingarro
Summary: The endoplasmic reticulum (ER) plays a crucial role in eukaryotic cells by facilitating the entry and distribution of newly synthesized proteins. Membrane-integrated amino acids are typically non-polar/hydrophobic and form alpha-helical transmembrane (TM) helices for survival in the ER's non-aqueous environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kerrie A. Morrison, Kate J. Heesom, Karen J. Edler, James Doutch, Gareth J. Price, Francoise Koumanov, Paul Whitley
Summary: Traditional extraction of membrane proteins involves detergents, but a new technique using SMA nanodiscs has been developed. While detergent extraction is more efficient, samples from SMA extraction had fewer nonspecific proteins and primarily contained multi-spanning membrane proteins. Further investigation into the qualitative differences between SMA and detergent extraction is warranted.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Kerrie A. Morrison, Laura Wood, Karen J. Edler, James Doutch, Gareth J. Price, Francoise Koumanov, Paul Whitley
Summary: Extraction of integral membrane proteins using poly(styrene-co-maleic acid) can retain the lipid bilayer surrounding the proteins. This study demonstrates efficient extraction of the mammalian insulin receptor with the copolymer but reveals a lack of functional response to insulin after extraction.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Shawna L. L. McMillin, Parker L. L. Evans, William M. M. Taylor, Luke A. A. Weyrauch, Tyler J. J. Sermersheim, Steven S. S. Welc, Monique R. R. Heitmeier, Richard C. C. Hresko, Paul W. W. Hruz, Francoise Koumanov, Geoffrey D. D. Holman, E. Dale Abel, Carol A. A. Witczak
Summary: The study found that GLUT1 does not mediate basal muscle glucose uptake and suggests the existence of a novel glucose transport mechanism for overload-stimulated glucose uptake.
Article
Biochemistry & Molecular Biology
Jennifer S. McDowall, Ioanna Ntai, Jonathon Hake, Paul R. Whitley, Jody M. Mason, Christopher R. Pudney, David R. Brown