Article
Plant Sciences
Eric Poliner, Andrea W. U. Busch, Linsey Newton, Young Uk Kim, Rachel Clark, Sofia C. Gonzalez-Martinez, Byeong-Ryool Jeong, Beronda L. Montgomery, Eva M. Farre
Summary: The study found that blue light induces the expression of fatty acid desaturase genes in stramenopile microalgae, promoting the synthesis of EPA. The promoters of blue light-induced genes are enriched with E-box-like motifs. Aureochromes, a specific protein in stramenopiles, act as blue light-regulated transcription factors by sensing blue light.
Review
Biotechnology & Applied Microbiology
Zijian Xie, Jiaxin He, Shuting Peng, Xiaoyun Zhang, Weibao Kong
Summary: Protein-based drugs are the fastest-growing category in the pharmaceutical industry, but their development has been hindered by low yields and high production costs. Eukaryotic microalgae offer advantages as a novel platform for protein-based drug production. This review highlights their potential, advantages, and disadvantages, and summarizes the characteristics of gene expression, translation, and post-translational modifications in eukaryotic microalgae. Additionally, it introduces practical and research advances in using eukaryotic microalgae for producing protein-based drugs.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Multidisciplinary Sciences
Yi-Ping Hsiang, Yi-Ting Wang, Keng-Shiang Huang, Ting-Yu Huang, Mi-Hsueh Tai, Yu-Mei Lin, Chih-Hui Yang, Jei-Fu Shaw
Summary: The study designed a chlorophyll purification method using twice solvent extraction to enhance the purity of total chlorophylls and convert them into chlorophyllides. The purified chlorophyllides showed good biocompatibility and anticancer activity in breast cancer cell lines, suggesting their potential as a candidate for combination therapy with doxorubicin.
Article
Biology
Felix Proulx-Giraldeau, Jan M. Skotheim, Paul Francois
Summary: Cell size control mechanisms are diverse and influenced by cell cycle structure and selection pressures. The evolution of these mechanisms can lead to the emergence of critical processes in complex systems.
Article
Plant Sciences
Yi-Ying Lee, Rudolph Park, Stephen M. Miller, Yantao Li
Summary: Genetic compensation is proposed as a mechanism to explain phenotypic differences between gene knockouts and knockdowns. This study demonstrates examples of genetic compensation in microalgae and suggests its physiological relevance in TAG biosynthesis under stress. These findings provide guidance for future genetic engineering and mutant characterization efforts.
Article
Multidisciplinary Sciences
Zihui Huang, Liangliang Shen, Wenda Wang, Zhiyuan Mao, Xiaohan Yi, Tingyun Kuang, Jian-Ren Shen, Xing Zhang, Guangye Han
Summary: Photosystems I and II undergo state transitions to balance light energy distribution and optimize photosynthesis, with the cryo-EM structure of a PSI-LHCI-LHCII supercomplex from C. reinhardtii revealing subunit organization and energy transfer pathways.
NATURE COMMUNICATIONS
(2021)
Article
Nutrition & Dietetics
Jin-peng Sun, Xue-hong Wei, Xiao-mei Cong, Wen-hua Zhang, Le-Xin Qiu, Xiao-nan Zang
Summary: In this study, a model for astaxanthin production similar to H. pluvialis was constructed in C. reinhardtii by combining carotenoid metabolism and fatty acid metabolism. It was found that lipids contribute to the synthesis of astaxanthin by regulating carbon flow, and the expression of the MECR gene related to long-chain fatty acid synthesis further increases the astaxanthin content.
FRONTIERS IN NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Jiale Xing, Junting Pan, Heng Yi, Kang Lv, Qiuliang Gan, Meimei Wang, Haitao Ge, Xiahe Huang, Fang Huang, Yingchun Wang, Jean-David Rochaix, Wenqiang Yang
Summary: This study investigates the function of the largest gene, orf2971, in Chlamydomonas reinhardtii chloroplasts. The findings suggest that Orf2971 interacts with the TIC complex and plays a role in the protein import machinery. Repression of Orf2971 leads to accumulation of chloroplast precursor proteins and impaired chloroplast quality.
Article
Biochemistry & Molecular Biology
Raphael Troesch, Fabian Ries, Lisa Desiree Westrich, Yang Gao, Claudia Herkt, Julia Hoppstaedter, Johannes Heck-Roth, Matthieu Mustas, David Scheuring, Yves Choquet, Markus Raeschle, Reimo Zoschke, Felix Willmund
Summary: Exposure to high temperatures causes temporary changes in chloroplast translation, specifically affecting antenna proteins of photosynthesis. This study reveals the sensitive regulatory principles involved in heat acclimation for the biogenesis and repair of chloroplast-encoded core subunits of photosynthetic complexes. The findings suggest that protein biosynthesis and translation elongation play central roles in heat acclimation and are conserved between different plant species.
Article
Multidisciplinary Sciences
David Bauer, Hiroaki Ishikawa, Kimberly A. Wemmer, Nathan L. Hendel, Jane Kondev, Wallace F. Marshall
Summary: Cell-to-cell variation is mainly influenced by cell size, while within-cell variation is caused by dynamic fluctuations. Tubulin assembly is not directly related to intraflagellar transport, and length fluctuations reflect tubulin assembly and disassembly events involving large numbers of tubulin dimers. Length variation increases in long-flagella mutants, consistent with theoretical models for flagellar length regulation.
Article
Engineering, Environmental
Haonan Fan, Shanghua Wu, Wenxu Dong, Xianglong Li, Yuzhu Dong, Shijie Wang, Yong-Guan Zhu, Xuliang Zhuang
Summary: This study identified antibiotic-resistant bacteria in soil-plant systems using stable isotope probing and highlighted the rhizosphere as a hotspot for antibiotic resistance gene transfer.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Multidisciplinary Sciences
Xiaodan Guan, Liang Jin, Huifen Zhou, Jing Chen, Haofang Wan, Yida Bao, Jiehong Yang, Daojun Yu, Haitong Wan
Summary: This study evaluated the inhibitory effects of polydatin on carbapenem-resistant Klebsiella pneumoniae (CRKP). The results showed that polydatin can inhibit CRKP biofilm formation and efflux pump expression, and suppress bacterial-induced cell damage by regulating antioxidant pathways.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Maria Teresa Giardi, Daniele Zappi, Mehmet Turemis, Gabriele Varani, Fabrizio Lo Celso, Giampaolo Barone, Pietro Calandra, Amina Antonacci, Viviana Scognamiglio
Summary: This passage describes a method to design and synthesize novel artificial peptides that mimic the plastoquinone binding niche of the D1 protein from Chlamydomonas reinhardtii, with the ability to bind herbicides. These peptides were then functionalized with quantum dots for the development of a hybrid optosensor for the detection of atrazine, meeting E.U. legislation requirements with detection limits in the mu g/L concentration range.
Review
Cell Biology
Jennifer F. Pinello, Theodore G. Clark
Summary: Most cellular requirements for fertilization and sexual reproduction arose early in evolution and are retained in single-celled organisms. Research on green algae and free-living ciliates has provided important insights into the role of HAP2/GCS1 as a catalyst for gamete fusion, and opportunities for controlling protozoan parasites.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Environmental
Ivan Liakh, Darya Harshkova, Pavel Hrouzek, Katerina Bisova, Anna Aksmann, Bartosz Wielgomas
Summary: The use of unicellular algae for removing xenobiotics from wastewaters is a rapidly developing field in environmental protection. Efficient phycoremediation requires biosorption, bioaccumulation, and biotransformation. However, the potential toxicity of biotransformation products and the resistance of analyzed algae strains to the mixture of compounds need to be considered. In this study, Chlamydomonas reinhardtii showed promise in removing diclofenac, a non-steroidal anti-inflammatory drug, through biosorption, bioaccumulation, and biotransformation processes.
JOURNAL OF HAZARDOUS MATERIALS
(2023)