Article
Chemistry, Multidisciplinary
Marjon Zamani, James M. Robson, Andy Fan, Michael S. Bono, Ariel L. Furst, Catherine M. Klapperich
Summary: Sexually transmitted infections, such as HIV and HPV, have a disproportionate impact on low-resource settings. Early diagnosis is crucial for managing HIV and preventing HPV-induced cervical cancer. A novel electrochemical platform combined with loop-mediated isothermal amplification and CRISPR-based detection shows promise for rapidly and cost-effectively detecting viral infections.
ACS CENTRAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Christian Kleinert, Carola Griehl
Summary: In situ extraction or milking of microalgae, particularly using strains like Showa and Bot22 of Botryococcus braunii, can lead to reduced downstream costs and increased efficiency in the production of low-value substances like lipids. This approach eliminates the need for costly steps like harvesting, dewatering, and cell disruption, making it a promising method for economic lipid production.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Koji Kawamura, Suzune Nishikawa, Kotaro Hirano, Ardianor Ardianor, Rudy Agung Nugroho
Summary: A DNAbased rapid estimation method for chemical races of B. braunii was developed. The dominant race was found to be B, with no A or S races isolated from tropical regions. Some ponds showed dominance of minor races or a mixture of different races.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Christian Kleinert, Carola Griehl
Summary: Milking microalgae is a promising method to reduce downstream costs by skipping certain steps in the production process. The green microalga Botryococcus braunii is well-suited for this due to its ability to accumulate large amounts of long-chain hydrocarbons extracellularly. Among the twelve Botryococcus braunii strains investigated, two strains - Showa and Bot22 - showed particular potential for successful milking with high productivity and extractant compatibility.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Multidisciplinary Sciences
Koji Kawamura, Suzune Nishikawa, Kotaro Hirano, Ardianor Ardianor, Rudy Agung Nugroho, Shigeru Okada
Summary: Algal biofuel research aims to produce a renewable, carbon-neutral biofuel using oil-producing microalgae. A large-scale screening of 180 strains from 22 different ponds identified 9 wild strains with faster growth rates than the benchmark strain Showa, with OIT-678 displaying the fastest growth rate and higher biomass and hydrocarbon productivities.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Mohsen Mohammadniaei, Ming Zhang, Jon Ashley, Ulf Bech Christensen, Lennart Jan Friis-Hansen, Rasmus Gregersen, Jan Gorm Lisby, Thomas Lars Benfield, Finn Erland Nielsen, Jens Henning Rasmussen, Ellen Botker Pedersen, Anne Christine Rye Olinger, Laerke Torring Kolding, Maryam Naseri, Tao Zheng, Wentao Wang, Jan Gorodkin, Yi Sun
Summary: The study introduces a novel non-enzymatic isothermal amplification method for rapid detection of SARS-CoV-2 RNA, without the need for reverse transcription, with high specificity and sensitivity. The method is simple, fast, cost-effective, stable, and suitable for a wide range of testing needs.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Yang Bu, Sheng Ni, Levent Yobas
Summary: This article investigates the mechanism of synchronous coefficient of drag alteration and finds that using a microfabricated artificial sieve can reduce the purification time of DNA from hours to minutes. An electrophoretic protocol is also proposed to facilitate the collection of purified DNA from the sieve, which can be used for rapid diagnostic applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Yuling Wang, Cheng Peng, Lin Ding, Zhixun Su, Xiaoyun Chen, Xiaofu Wang, Meihao Sun, Junfeng Xu
Summary: We developed a fluorescence visualization detection method based on PCR and CRISPR/Cas12a approaches for detecting the nopaline synthase terminator (T-nos) in genetically modified crops. The method has high sensitivity and stability, and does not require expensive instruments or technicians, making it suitable for mass detection of transgenic components in plants in the field.
Article
Biochemical Research Methods
Xiaoli Zhao, Yong Li, Ritong Sun, Yaofang Fan, Xiaofeng Mu, Ye Wang, Chao Shi, Cuiping Ma
Summary: This study presents a novel strategy for miRNA extraction based on electrical potential-assisted DNA-RNA hybridization. The entire extraction process is rapid and sensitive, with the advantages of simplicity and specificity. Satisfactory results were achieved for miRNA extraction in serum.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Chang Liu, Siyu Gou, Yanhui Bi, Qi Gao, Juanjuan Sun, Shanjin Hu, Weiwei Guo
Summary: A portable, cost-effective and storable DNA-gold nanoparticle hybrid hydrogel film biosensing system was developed, which exhibited high sensitivity and fast response for Pb2+ and UO22+ detection. This system has great potential for future rapid on-site detection applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Hye-sun Cho, Munjeong Choi, Yunjung Lee, Hyoim Jeon, Byeongyong Ahn, Nagasundarapandian Soundrarajan, Kwonho Hong, Jin-Hoi Kim, Chankyu Park
Summary: The study found that using broad-spectrum antimicrobial peptide PMAP-36 can effectively isolate high-quality RNA from various bacteria, providing an efficient method for nucleic acid isolation. Additionally, the use of PMAP-36 also successfully isolates high-quality genomic DNA from challenging bacterial strains, offering a new option for microbiome research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Yao Yin, Wei Xie, Mengyi Xiong, Yingying Gao, Qin Liu, Da Han, Guoliang Ke, Xiao-Bing Zhang
Summary: Inspired by the naturally confined enzymes on fluid cell membranes, a fluidly confined CRISPR-based DNA reporter (FINDER) was developed on living cell membranes, which was successfully applied for rapid and sensitive cancer cell identification in clinical blood samples. This new phenomenon was then combined with multiple aptamer-based DNA logic gate for cell recognition, thus a FINDER system capable of accurate, rapid and sensitive cancer cell identification was constructed.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biotechnology & Applied Microbiology
Rochelle Payne Ondracek, Jianhong Chen, Beth Marosy, Sirinapa Szewczyk, Leonard Medico, Amrutha Sherly Mohan, Priya Nair, Rachel Pratt, Janise M. Roh, Thaer Khoury, John Carpten, Lawrence H. Kushi, Julie R. Palmer, Kim Doheny, Warren Davis, Michael J. Higgins, Song Yao, Christine B. Ambrosone
Summary: This study reports the results and lessons learned from processing FFPE breast tumor tissues for a large epidemiologic study. The dual DNA/RNA purification using Qiagen AllPrep FFPE extraction protocol is feasible for clinical and epidemiologic studies. The use of tissue punches as a reliable source material and the fine tuning of proteinase K digestion time based on tissue volume are critical for achieving balanced yields of DNA and RNA.
Article
Multidisciplinary Sciences
Jon Bohlin
Summary: The nucleotide composition of a genome can affect an organism's environment and selective processes. This study presents a model that explores the evolution of genomic GC content over time. Random perturbations in mutation rates can lead to increased nucleotide variability. The implications of this finding are significant for resource allocation and adaptation to variable environments.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Yao Li, Sha Liu, Yuanyuan Wang, Yue Wang, Song Li, Nongyue He, Yan Deng, Zhu Chen
Summary: This article presents a comprehensive design scheme for a rapid automated nucleic acid extraction system based on magnetic separation. The study and evaluation of the system demonstrate its high temperature stability, positioning accuracy, and nucleic acid recovery rate. The system is capable of extracting highly pure nucleic acids from different samples, meeting the requirements of genetic-level research.
Article
Biotechnology & Applied Microbiology
Jin-Ho Yun, Dae-Hyun Cho, Bongsoo Lee, Yong Jae Lee, Dong-Yun Choi, Hee-Sik Kim, Yong Keun Chang
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2020)
Article
Microbiology
Sang-Ah Lee, Ve Van Le, So-Ra Ko, Nakyeong Lee, Hee-Mock Oh, Chi-Yong Ahn
Summary: The bacterial strain HC2(T) isolated from the phycosphere of Haematococcus lacustris NIES 144 culture belongs to the genus Mucilaginibacter and represents a novel species named Mucilaginibacter inviolabilis sp. nov. HC2(T) can grow at pH 4.5-8.0, at 4-32 degrees C and in the presence of 0-2 % NaCl.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
So-Ra Ko, Ve Van Le, Long Jin, Sang-Ah Lee, Chi-Yong Ahn, Hee- Mock Oh
Summary: A novel Gram-stain-negative bacterium, Mariniflexile maritimum, was isolated from seawater collected from the South Sea of Korea. Through 16S rRNA gene sequence similarity and genome-based phylogenetic analyses, it was found that this strain formed a distinct cluster within the genus Mariniflexile.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Minh Kim Nguyen, Vu Khac Hoang Bui, Chi-Yong Ahn, Hee-Mock Oh, Jin-Soo Koh, Ju-Young Moon, Young-Chul Lee
Summary: The study focuses on controlling and mitigating harmful algal blooms using aminoclays, with Anabaena showing higher sensitivity to the ACs. The algicidal activity is most effective in the order of aluminum aminoclay > magnesium aminoclay > calcium aminoclay.
APPLIED SCIENCES-BASEL
(2021)
Review
Agricultural Engineering
Aswathi Mohan, Aiswarya Robert Antony, Kozhumal Greeshma, Jin-Ho Yun, Rishiram Ramanan, Hee-Sik Kim
Summary: The review examines algae-derived natural, carbon neutral polysaccharides and polyesters, their production and biodegradability, and suggests accelerating research in the field of eco-friendly polymers. It emphasizes improving cost-effectiveness of algal biorefineries by coupling biofuel, high-value products, and biopolymer production.
BIORESOURCE TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Ve Van Le, Quynh-Giao Tran, So-Ra Ko, Sang-Ah Lee, Hee-Mock Oh, Hee-Sik Kim, Chi-Yong Ahn
Summary: This review summarizes the effects of antibiotic pollution on freshwater microalgae and cyanobacteria, focusing on major biological processes such as photosynthesis, oxidative stress, and macromolecule metabolism. Adaptive mechanisms and antibiotic removal pathways by these organisms are also discussed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Microbiology
Ve Van Le, So-Ra Ko, Sang-Ah Lee, Long Jin, Jochen Blom, Chi-Yong Ahn, Hee-Mock Oh
Summary: A novel algicidal bacterium, strain M26A2M(T), shows high potential in controlling harmful algal blooms caused by C. polykrikoides, by disrupting membrane integrity and the photosynthetic system of the algae cells. Its taxonomic position within the Rhodobacteraceae family is proposed as a new species under the name Cochlodiniinecator piscidefendens.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Ve Van Le, So-Ra Ko, Mingyeong Kang, Sang-Ah Lee, Hee-Mock Oh, Chi-Yong Ahn
Summary: A rod-shaped bacterial strain DH6(T) was isolated from freshwater in the Daechung Reservoir, Korea, and belongs to the novel species Panacibacter microcysteis sp. nov. This strain showed high tolerance to various environmental conditions, including pH, temperature, and salt concentration.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Environmental Sciences
Ve Van Le, So-Ra Ko, Mingyeong Kang, Sang-Ah Lee, Hee-Mock Oh, Chi-Yong Ahn
Summary: Paucibacter strain DH15 exhibits algicidal activity against Microcystis aeruginosa and has the capability to degrade microcystin. The study suggests that DH15 strain has great potential to control Microcystis blooms.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Ve Van Le, So-Ra Ko, Mingyeong Kang, Chan-Yeong Park, Sang-Ah Lee, Hee -Mock Oh, Chi -Yong Ahn
Summary: This study investigated the effectiveness of Paucibacter aquatile DH15 in controlling Microcystis blooms. The results showed that DH15 significantly reduced Microcystis cell density, decreased microcystins release, and had an impact on water quality and microbial community.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
Long Jin, Chengda Cui, Chengxiao Zhang, So-Ra Ko, Taihua Li, Feng-Jie Jin, Chi-Yong Ahn, Hee-Mock Oh, Hyung-Gwan Lee
Summary: A microcystin-degrading bacterial strain, Blastomonas fulva T2, was isolated and its complete genome was sequenced. The strain exhibited enzymes for microcystin degradation and multiple efflux pumps. These findings are important for understanding the degradation mechanism of microcystin and the evolutionary and physiological roles of bacterial multidrug efflux systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Kozhumal Greeshma, Hee-Sik Kim, Rishiram Ramanan
Summary: The study highlights the importance of microbial communities in the removal of heavy metals, especially the synergistic effects of cooperation between different microbial taxa.
ENVIRONMENTAL RESEARCH
(2022)
Article
Engineering, Environmental
Rishiram Ramanan, C. Amaneesh, Shankari Anna Balan, P. S. Silpa, Ji Won Kim, Kozhumal Greeshma, A. Aswathi Mohan, Aiswarya Robert Antony, Hans-Peter Grossart, Hee-Sik Kim
Summary: Plastic debris is a major environmental threat to aquatic systems globally. Microplastics and plastic leachates have been found in human organs, posing severe health hazards. These pollutants have been detected in various aquatic ecosystems, but their impacts on primary producers have not been fully studied.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Ve Van Le, So-Ra Ko, Mingyeong Kang, Hee-Mock Oh, Chi-Yong Ahn
Summary: Global warming and eutrophication have accelerated Microcystis blooms, deteriorating water quality. Cyanocides produced by cyanobactericidal bacteria have shown potential for controlling Microcystis blooms due to their environmental friendliness. In this study, a novel macrocyclic tambjamine called paucibactin A was isolated and found to have cyanocidal activity against Microcystis, selectively targeting it and Dolichospermum in microcosm studies.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Microbiology
So-Ra Ko, Ve Van Le, Mingyeong Kang, Hee-Mock Oh, Chi-Yong Ahn
Summary: A rod-shaped, non-motile, Gram-negative bacterium designated as strain RS28T was isolated from rice straw used for periphyton growth. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain RS28T belonged to the genus Mucilaginibacter and had the highest sequence similarity with Mucilaginibacter ginkgonis HMF7856T (96.47%) and Mucilaginibacter polytrichastri DSM 26907T (96.12%). Strain RS28T exhibited a broad pH and temperature range for growth and could tolerate up to 1.5% (w/v) NaCl. The fatty acid composition of strain RS28T primarily consisted of summed feature 3, iso-C15:0, and iso-C17:0 3 -OH. Based on phenotypic, chemotaxonomic, and phylogenetic characteristics, strain RS28T represents a novel species of the genus Mucilaginibacter, named Mucilaginibacter straminoryzae sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
