Article
Immunology
Ezhaveni Sathiyamoorthi, Jin-Hyung Lee, Yulong Tan, Jintae Lee
Summary: This study investigated the antibacterial and antivirulence effects of chromones and their derivatives against Vibrio parahaemolyticus and Vibrio harveyi. 6-Bromo-3-formylchromone (6B3FC) and 6-chloro-3-formylchromone (6C3FC) were found to be active against these bacteria and inhibited biofilm formation. 6B3FC also showed inhibition of gene expression associated with quorum sensing and biofilm formation in V. parahaemolyticus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Qian Chen, Bangguo Ma, Mingzhe Xu, Huiwen Xu, Zimiao Yan, Fei Wang, Yiran Wang, Zitong Huang, Shuchang Yin, Yancui Zhao, Lei Wang, Hongyan Wu, Xiaoli Liu
Summary: In this study, exosomes were isolated from Vibrio harveyi and Vibrio anguillarum using a supercentrifugation method, and their protein compositions were analyzed. The exosomes of these two vibrios contained virulence factors and also played roles in important bacterial activities. Further experiments confirmed the involvement of exosomes in vibrio toxicity. This study provides a valuable proteome database for understanding the pathogenic mechanisms of vibrios from the exosome perspective.
MICROBIAL PATHOGENESIS
(2023)
Article
Environmental Sciences
Ruixuan Wang, Xiaozhi Lin, Guangcai Zha, Juan Wang, Wei Huang, Jiangyong Wang, Yuee Hou, Hongli Mou, Ting Zhang, Hui Zhu, Jun Wang
Summary: Vibrio harveyi infection in aquaculture has caused significant economic losses. This study found that a pathogenic strain of V. harveyi could be induced to become a multiple-resistant strain by enrofloxacin, and the mechanism of multidrug resistance was mainly related to the upregulation of the efflux and SOS systems. This research is important for addressing the issue of drug resistance in V. harveyi.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Ameya A. Mashruwala, Boyang Qin, Bonnie L. Bassler
Summary: Bacterial colonies composed of genetically identical individuals can diversify through T6SS-mediated cell death, resulting in variant cells with distinct genotypes. This variant outgrowth is regulated by quorum sensing and T6SS-driven killing, promoting genetic diversity in bacteria.
Article
Fisheries
Liting Wu, Yuan Tian, Maoda Pang, Zhenquan Yang, Hongduo Bao, Yan Zhou, Lichang Sun, Ran Wang, Hui Zhang
Summary: The study investigated the phenotypic characterization and antimicrobial susceptibility patterns of 54 V. harveyi strains collected from Larimichthys crocea in China. The isolates displayed resistance to a variety of antibiotics and carried multiple virulence genes. Additionally, a novel V. harveyi-specific phage with broad host range was discovered and showed high efficacy in preventing and controlling V. harveyi infection in a zebrafish model.
Article
Multidisciplinary Sciences
Michaela Huber, Anne Lippegaus, Sahar Melamed, Malte Siemers, Benjamin R. Wucher, Mona Hoyos, Carey Nadell, Gisela Storz, Kai Papenfort
Summary: Using RIL-seq, researchers have identified Hfq-interacting sRNAs and their target sites in Vibrio cholerae, finding numerous sRNA-mRNA interactions and RNA duplexes formed by sRNA regulators. Further analysis reveals an RNA sponge called QrrX that base-pairs with and inactivates the Qrr1-4 sRNAs, which are known to modulate the quorum sensing pathway. Transcription of qrrX is activated by QrrT, a previously uncharacterized LysR-type transcriptional regulator.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Michaela Huber, Anne Lippegaus, Sahar Melamed, Malte Siemers, Benjamin R. Wucher, Mona Hoyos, Carey Nadell, Gisela Storz, Kai Papenfort
Summary: The study reveals the interaction between small regulatory RNAs (sRNAs) and the RNA-chaperone Hfq in Vibrio cholerae, a human pathogen. By using RIL-seq, the researchers identify multiple sRNA-mRNA interactions as well as RNA duplexes formed between two sRNA regulators. They also discover an RNA sponge called QrrX, which can bind and inactivate the Qrr1-4 sRNAs that modulate the quorum sensing pathway. The results indicate the importance of QrrX and QrrT in the rapid transition from individual to community behaviors in V. cholerae.
NATURE COMMUNICATIONS
(2022)
Article
Fisheries
Hongtao Liu, Shengtao Guo, Rong Wang, Yugui He, Qiong Shi, Zhaobin Song, Mingqiu Yang
Summary: The study used proteomic sequencing of hepatopancreas in Vibrio harveyi-infected whiteleg shrimp and found a significant number of DEPs, specifically showing changes in the expression levels of C-type lectins after infection. Different members of the CTL family exhibit varied responses to Vibrio harveyi infection, suggesting potential differential functions and providing theoretical guidance for further research on immune responses and pathogen infections in shrimps.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Microbiology
Yiqin Deng, Shujun Zang, Ziyang Lin, Liwen Xu, Changhong Cheng, Juan Feng
Summary: Hfq is a global regulator that plays an important role in virulence regulation in Vibrio harveyi. It positively regulates bacterial motility and biofilm formation, and is involved in flagellar assembly and bacterial chemotaxis by binding small regulatory RNAs and regulating target mRNAs.
Article
Food Science & Technology
Ezhaveni Sathiyamoorthi, Olajide Sunday Faleye, Jin-Hyung Lee, Jintae Lee
Summary: This study investigated the antibacterial, antibiofilm, and antivirulence activities of hydroquinone (HQ) and its derivatives against V. parahaemolyticus and V. harveyi. The compounds 2,3-DMHQ and DBHQ showed significant antibacterial and antibiofilm effects, indicating their potential for controlling V. parahaemolyticus in food production facilities.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Adriana Triga, Maria Smyrli, Pantelis Katharios
Summary: A monitoring program has been implemented in Greek marine aquaculture for the past 13 years to track vibriosis incidents. A total of 273 isolates were collected and characterized from various cases involving eight regions and nine hosts. Vibrio harveyi was found to have the highest prevalence and was isolated year-round from all hosts. Other Vibrio species, such as Vibrio lentus, Vibrio cyclitrophicus, and Vibrio gigantis, were more abundant during the spring season. The severity of the disease and the frequency of outbreaks make vibriosis a significant concern for the regional aquaculture sector.
Article
Microbiology
Ming Xue, Qi Gao, Rui Yan, Lingping Liu, Ling Wang, Binyu Wen, Chongqing Wen
Summary: This study characterized the virulence factor repertoire of V. parahaemolyticus strain LC, which showed significant virulence to shrimp Litopenaeus vannamei. The results revealed the presence of specific genes associated with shrimp and human pathogenic strains, as well as unique mobile genetic elements-borne virulence factors. V. parahaemolyticus demonstrates host-specific virulence, contributing to the origin and spread of pathogenic factors.
Article
Fisheries
Chinnadurai Lelin, Eswaramoorthy Thirumalaikumar, Ganapathi Uma, Mariavincent Michael Babu, Chellappan Ajan, Sugumar Vimal, Thavasimuthu Citarasu
Summary: The isolated bacteriophage from the semi-intensive culture of Pacific white leg shrimp has strong lytic activity against Vibrio harveyi and other pathogenic vibrios, inhibits biofilm formation, and shows specific activity against V. harveyi infections.
AQUACULTURE INTERNATIONAL
(2022)
Article
Environmental Sciences
Md. Ashrafudoulla, Kyung Won Na, Md. Iqbal Hossain, Md. Furkanur Rahaman Mizan, Shamsun Nahar, Sazzad Hossen Toushik, Pantu Kumar Roy, Si Hong Park, Sang -Do Ha
Summary: Infections of gastroenteritis in humans are mainly caused by consumption of shellfish contaminated with Vibrio parahaemolyticus, leading to health and economic losses. Investigation on the virulence factor production, antibiotic resistance profile, and biofilm-forming capacity of Vibrio parahaemolyticus isolates showed high resistance to antibiotics and virulence factors in isolates from shellfish in Korea. Monitoring of these factors may help in decreasing V. parahaemolyticus infections and ensuring seafood safety.
MARINE POLLUTION BULLETIN
(2021)
Article
Multidisciplinary Sciences
Ling-Chun Lin, Yu-Chuan Tsai
Summary: The study characterized the potentially pathogenic Vibrio owensii GRA50-12 and isolated and identified its virulent phage phi50-12 from the same habitat, demonstrating phi50-12 as a potential candidate for treating V. owensii infection or as a biocontrol agent. The research further highlighted the features and potential of phi50-12.
SCIENTIFIC REPORTS
(2022)
