Article
Immunology
Helena Nunes-Cabaco, Andreia Ramalho-dos-Santos, Ana R. Pires, Leila R. Martins, Joao T. Barata, Ana E. Sousa
Summary: IL-4 plays a role in the regulation of human thymocyte plasticity and is involved in the generation of CD8+ T cells in the thymus. IL-4 also has implications in neonatal immunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Jennifer Massey, Katherine Jackson, Mandeep Singh, Brendan Hughes, Barbara Withers, Carole Ford, Melissa Khoo, Kevin Hendrawan, John Zaunders, Benedicte Charmeteau-De Muylder, Remi Cheynier, Fabio Luciani, David Ma, John Moore, Ian Sutton
Summary: This study investigates T cell reconstitution in highly active MS patients for 36 months after AHSCT. The results show that AHSCT induces significant changes in the dominant T cell clones of CD4+ and CD8+ memory T cells. After lymphopenia-induced homeostatic proliferation, clonal attrition occurs. Recovery of thymically-derived CD4 naive T cell repertoire begins at 12 months and continues, but the diversity of the naive populations does not increase compared to baseline, indicating that the principal mechanism for durable remission from MS after AHSCT relates to depletion of putative auto-reactive clones.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Armanda Viana Rodrigues, Ana Valerio-Bolas, Graca Alexandre-Pires, Maria Aires Pereira, Telmo Nunes, Dario Ligeiro, Isabel Pereira da Fonseca, Gabriela Santos-Gomes
Summary: This study compares the innate immune response to L. infantum infection of two different macrophage lineages: blood macrophages and liver macrophages (Kupffer cells). The findings show that blood macrophages are susceptible to parasite infection and the parasites quickly subvert the immune mechanisms of these cells. On the other hand, liver macrophages are not extensively activated upon initial contact with the parasite but appear to be more efficient in managing parasite infection, contributing to the liver's ability to naturally restrain Leishmania dissemination.
Letter
Allergy
Claudia Varandas, Maria Conceicao Pereira-Santos, Marta Neto, Ana E. Sousa, Anabela Lopes, Susana L. Silva
CLINICAL AND EXPERIMENTAL ALLERGY
(2022)
Article
Immunology
Joao Canto-Gomes, Carolina S. Silva, Rita Rb-Silva, Daniela Boleixa, Ana Martins da Silva, Remi Cheynier, Patricio Costa, Ines Gonzalez-Suarez, Margarida Correia-Neves, Joao J. Cerqueira, Claudia Nobrega
Summary: The aim of this study is to assess the peripheral immune system of newly diagnosed patients with relapsing remitting multiple sclerosis (RRMS) and identify immune features associated with clinical onset.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Adriana Motta-Raymundo, Pedro Rosmaninho, Diana F. Santos, Ruben D. Ferreira, Sara P. Silva, Cristina Ferreira, Ana E. Sousa, Susana L. Silva
Summary: Helicobacter pylori (HP) infection may contribute to the clinical and immune phenotype of Common Variable Immunodeficiency (CVID). HP mainly affects the gastric mucosa and increases the incidence of early onset gastric cancer in CVID patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Alexandre Nicolas, Julie Migraine, Jacques Dutrieux, Maud Salmona, Alexandra Tauzin, Atsuko Hachiya, Jerome Estaquier, Jean-Michel Molina, Francois Clavel, Allan J. Hance, Fabrizio Mammano
Summary: In this study, the genotypic and phenotypic diversity of replication-competent near-full-length HIV genomes were explored in eight patients with different delays in viremia control and treatment durations. The results showed that delayed effective treatment was associated with increased genetic diversity of the reservoir. The duration of treatment did not impact the diversity, but was associated with a higher frequency of clonally expanded sequences. Therefore, early treatment initiation has the advantage of reducing both the size and diversity of the reservoir.
MICROBIOLOGY SPECTRUM
(2022)
Article
Hematology
Andre M. C. Gomes, Guilherme B. Farias, Amelia C. Trombetta, Ana Godinho-Santos, Ines Parreira, Helder Diogo Goncalves, Mariana Lessa Simoes, Patricio Aguiar, Maria Manuel Deveza, Joao Inacio, Ana E. Sousa, Susana Lopes da Silva
Summary: An XLA patient with a 7-month course of COVID-19 showed persistent plasma SARS-CoV-2 load. The myeloid cells exhibited a sustained non-inflammatory profile, which was associated with contained severity of the disease. This suggests the potential benefit of using BTK inhibitors in SARS-CoV-2 infection.
EUROPEAN JOURNAL OF HAEMATOLOGY
(2023)
Article
Cell Biology
Thomas Andrew Fox, Benjamin Christopher Houghton, Lina Petersone, Erin Waters, Natalie Mona Edner, Alex McKenna, Olivier Preham, Claudia Hinze, Cayman Williams, Adriana Silva de Albuquerque, Alan Kennedy, Anne Maria Pesenacker, Pietro Genovese, Lucy Sarah Kate Walker, Siobhan Oisin Burns, David Michael Sansom, Claire Booth, Emma Catherine Morris
Summary: A gene editing strategy was designed to insert the CTLA-4 cDNA into primary human T cells, restoring CTLA-4 expression and demonstrating the feasibility of using T cell gene therapy for CTLA-4 insufficiency.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Vanessa Daza-Cajigal, Adriana S. Albuquerque, Dan F. Young, Michael J. Ciancanelli, Dale Moulding, Ivan Angulo, Valentine Jeanne-Julien, Jeremie Rosain, Ekaterina Minskaia, Jean-Laurent Casanova, Stephanie Boisson-Dupuis, Jacinta Bustamante, Richard E. Randall, Timothy D. McHugh, Adrian J. Thrasher, Siobhan O. Burns
Summary: Partial JAK1 deficiency predominantly affects susceptibility to mycobacterial infection through impact on the IFN-gamma responsive pathway in myeloid cells. Susceptibility to viral infections as a result of reduced IFN-alpha responses is variable depending on cell type.
FRONTIERS IN IMMUNOLOGY
(2022)
Letter
Immunology
Adriana S. Albuquerque, Jesmeen Maimaris, Alexander J. McKenna, Jonathan Lambourne, Fernando Moreira, Sarita Workman, Karyn Megy, Ilenia Simeoni, Hana Lango Allen, NIHR BioResource-Rare Dis Consortium, Emma C. Morris, Siobhan O. Burns
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2023)
Article
Immunology
Andrey Velichkov, Rumyana Susurkova, Mariya Muhtarova, Margarita Guenova, Benedicte Charmeteau-de Muylder, Remi Cheynier, Gueorgui Nikolov, Emiliana Konova, Velislava Terzieva
Summary: Unexplained infertility is a significant challenge for clinicians and researchers and has a major social impact. This study focuses on the subset of regulatory T cells and their involvement in maternal tolerance towards the fetus. The findings indicate group-specific properties in the CD45RA+ T cell compartment between healthy parous, nulliparous, and women with unexplained infertility.
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Helene M. Roux, Amira Marouf, Jacques Dutrieux, Benedicte Charmeteau-De Muylder, Suzanne Figueiredo-Morgado, Veronique Avettand-Fenoel, Pelagia Cuvelier, Cecile Naudin, Fatma Bouaziz, Guillaume Geri, Anne Couedel-Courteille, Pierre Squara, Stefano Marullo, Remi Cheynier
Summary: Thymic activation improves the outcome of COVID-19 patients with severe pneumonia. Genetic polymorphism that affects thymic output can modify SARS-CoV-2 immunity and disease severity. GG genotype is associated with stronger and long-lasting immune response and less severe lung involvement.
Article
Biochemical Research Methods
Helene Marie Roux, Suzanne Figueiredo, Lucas Sareoua, Maud Salmona, Juliette Hamroune, Lucie Adoux, Julie Migraine, Allan Hance, Francois Clavel, Remi Cheynier, Jacques Dutrieux
Summary: A new technology called DUSQ was developed to quantitate unintegrated HIV DNA molecules with high sensitivity, specificity, and throughput. By combining linker-mediated PCR and next-generation sequencing (NGS) with molecular barcodes, DUSQ can detect and quantify unintegrated linear DNAs (ULDs). The research revealed that the half-life of ULDs in resting CD4+ T cells can be as long as 11 days. Moreover, DUSQ successfully quantified ULDs in samples from HIV-1 infected patients, demonstrating its potential for tracking pre-integrative latency in vivo. DUSQ technology can also be adapted for the detection of other rare DNA molecules.
CELL REPORTS METHODS
(2023)
Article
Immunology
Manuel M. Vicente, Ines Alves, Angela Fernandes, Ana M. Dias, Beatriz Santos-Pereira, Elena Perez-Anton, Sofia Santos, Tao Yang, Alexandra Correia, Anja Muenster-Kuehnel, Afonso R. M. Almeida, Sarina Ravens, Gabriel A. Rabinovich, Manuel Vilanova, Ana E. Sousa, Salome S. Pinho
Summary: The development of T-cells is regulated by glycosylation, which plays an important role in immune function and disease susceptibility. By studying the glycome compositions and dynamic alterations in human and murine thymocytes, it was discovered that high-mannose glycosylation is crucial for normal T-cell development. Specific glycoengineered mouse models further demonstrated that mannosylated thymocytes lead to developmental defects and increased susceptibility to inflammation.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)