Article
Biochemical Research Methods
Shibo Wang, Rui Wang, George Q. Li, Jun-Lae Cho, Yulin Deng, Yujuan Li
Summary: The study found that simulated microgravity for 21 days damaged the intestinal barrier, leading to decreased numbers of goblet cells, enlarged intercellular space, down-regulated adhesion molecules, and increased intestinal permeability. Proteomic analysis revealed that simulated microgravity significantly down-regulated adhesion molecules, deteriorated metabolic pathways, focal adhesion pathways, and the regulation of actin cytoskeleton pathways.
JOURNAL OF PROTEOMICS
(2021)
Article
Oncology
Fan Wu, Hui Li, Heng Zhang, Yusheng Liao, Huanping Ren, Jie Wu, Dan Zheng
Summary: In a D-GalN/LPS-induced ALF model, TNF-alpha and IL-6 signaling may increase MLCK and ROCK expression levels, further mediate phosphorylation of MLC, resulting in tight junction dysregulation and intestinal barrier dysfunction.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Critical Care Medicine
Chao Qin, Yi Jiang, Xing Chen, Yingxue Bian, Yaoqi Wang, Keliang Xie, Yonghao Yu
Summary: The study showed that dexmedetomidine treatment mitigated burn-induced intestinal barrier injury by inhibiting the MLCK/p-MLC signaling pathways, reducing histological damage, enhancing tight junction protein expression, and decreasing inflammation levels.
Article
Environmental Sciences
Lianxin Li, Jinge Xin, Hesong Wang, Yadong Wang, Weiqi Peng, Ning Sun, Haonan Huang, Yanxi Zhou, Xingmei Liu, Yu Lin, Jing Fang, Bo Jing, Kangcheng Pan, Yan Zeng, Dong Zeng, Xiang Qin, Yang Bai, Xueqin Ni
Summary: Fluoride is a common contaminant that poses challenges to animal and human health. This study investigates the role of the cytoskeleton in fluoride-induced barrier dysfunction and provides potential therapeutic targets for fluoride-induced intestinal injury.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Guo-Ping Zhao, Xiao-Yu Wang, Jin-Wang Li, Ran Wang, Fa-Zheng Ren, Guo-Fang Pang, Yi-Xuan Li
Summary: The study revealed that the neonicotinoid pesticide imidacloprid disrupts the permeability of the intestinal epithelial barrier, leading to an inflammatory response and down-regulation of tight junction proteins, especially involving the pregnane X receptor. This highlights the importance of considering the intestinal barrier disruption caused by IMI in assessing the safety of this class of pesticides.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Agriculture, Multidisciplinary
Juan Wang, Hui Zhao, Ke Lv, Wei Zhao, Ning Zhang, Fan Yang, Xiang Wen, Xiaohua Jiang, Jingrui Tian, Xinjuan Liu, Chi-Tang Ho, Shiming Li
Summary: Pterostilbene (PTE) protects intestinal epithelial barrier integrity through the NF-kappa B-MLCK/p-MLC signaling pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Joo-Yeon Lee, Choon Young Kim
Summary: This study demonstrates the preventive effect of thinned apple extracts (TAE) on TNF-alpha-induced intestinal epithelial barrier dysfunction. TAE improves tight junction integrity, inhibits interaction between ZO-1 and occludin, and suppresses myosin light chain kinase and NF-kappa B p65 protein levels.
Article
Agriculture, Multidisciplinary
Shuiping Liu, Jinyan Li, Weili Kang, Yun Li, Lei Ge, Dandan Liu, Yunhuan Liu, Kehe Huang
Summary: This study revealed that AFB1 induces intestinal barrier dysfunction through the FXR-mediated MLCK signaling pathway, providing novel insights into the prevention of mycotoxin poisoning in the intestine.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biology
Liwen Du, Leilei Zhu, Xiaozhen Lu, Yuezhou Yu, Peng Liu, Jianneng Pan
Summary: Inhibiting myosin light chain kinase (MLCK) can improve heat stroke-induced intestinal injury in rats by reducing intestinal permeability and protecting the intestinal mucosal barrier. This study demonstrates that inhibiting the MLCK/p-MLC2 signaling pathway may serve as a novel therapeutic approach for heat stroke-induced intestinal damage.
JOURNAL OF THERMAL BIOLOGY
(2023)
Article
Food Science & Technology
Wannaporn Chayalak, Chatsri Deachapunya, Sunit Suksamran, Sutthasinee Poonyachoti
Summary: The study showed that Hydroxyxanthones have a protective effect on preventing inflammation-induced TJ barrier disruption, making them a potential candidate for treating diseases related to leaky gut.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Cardiac & Cardiovascular Systems
Tatsuro Hitsumoto, Osamu Tsukamoto, Ken Matsuoka, Junjun Li, Li Liu, Yuki Kuramoto, Shuichiro Higo, Shou Ogawa, Noboru Fujino, Shohei Yoshida, Hidetaka Kioka, Hisakazu Kato, Hideyuki Hakui, Yuki Saito, Chisato Okamoto, Hijiri Inoue, Jo Hyejin, Kyoko Ueda, Takatsugu Segawa, Shunsuke Nishimura, Yoshihiro Asano, Hiroshi Asanuma, Akiyoshi Tani, Riyo Imamura, Shinsuke Komagawa, Toshio Kanai, Masayuki Takamura, Yasushi Sakata, Masafumi Kitakaze, Jun-ichi Haruta, Seiji Takashima
Summary: This study demonstrates that dysregulation of cMLCK contributes to the development of cardiac systolic dysfunction in humans, and restoring cMLCK activity could serve as a novel myotropic therapy for advanced systolic heart failure.
Review
Peripheral Vascular Disease
Masaaki Ito, Ryuji Okamoto, Hiromasa Ito, Ye Zhe, Kaoru Dohi
Summary: Regulation of muscle contraction is essential in the cardiovascular system, with the interaction between myosin and actin filaments being a common mechanism. The phosphorylation of myosin regulatory light chain plays a crucial role in smooth muscle contraction, while in cardiac muscle, troponin C is the primary target protein for Ca2+ with RLC phosphorylation also modulating contraction. Recent advances have improved our understanding of the regulatory role of RLC phosphorylation in smooth and cardiac muscles.
HYPERTENSION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Menglu Xi, Guo Hao, Qi Yao, Xuchang Duan, Wupeng Ge
Summary: The use of antibiotics early in life can lead to intestinal disorders in infants, but this study found that supplementing with galactooligosaccharides (GOS) could promote the growth of beneficial bacteria, improve intestinal barrier function, and reduce inflammation.
Article
Biochemistry & Molecular Biology
Dequan Kong, Baoying Xie, Yuhang Li, Yaping Xu
Summary: Palmitoylethanolamide (PEA) acts as a ROCK2 inhibitor to protect against BBB disruption independently of the PPAR alpha mechanism. PEA reduces ROCK/MLC signaling activation and stress fiber formation, alleviating early endothelial cell barrier disruption after oxygen-glucose deprivation. This study reveals a novel role of PEA and proposes an alternative PPAR alpha-independent mechanism for its pharmacological effects.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Oncology
Jingyin Zhang, Yingjian Jiang, Hongbo Li, Jiang Wang, Chang Li, Dianliang Zhang
Summary: The study investigates the effect and mechanism of heme oxygenase-1 (HO-1) on intestinal barrier damage in severe acute pancreatitis (SAP). The results suggest that upregulation of HO-1 expression can attenuate SAP-induced intestinal mucosal barrier damage, which is achieved through the inhibition of MLCK/p-MLC signaling pathway.
EXPERIMENTAL CELL RESEARCH
(2023)