Article
Plant Sciences
Huimin Zhao, Yani Niu, Hao Dong, Yaqi Jia, Yucheng Wang
Summary: The study identified two S1Fa-like TF genes, PtS1Fa1 and PtS1Fa2, in Populus trichocarpa. While PtS1Fa1 did not show significant effects, overexpression of PtS1Fa2 increased antioxidant activity and reduced ROS accumulation, playing a crucial role in drought tolerance in the plant.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Michael F. Nagle, Jialin Yuan, Damanpreet Kaur, Cathleen Ma, Ekaterina Peremyslova, Yuan Jiang, Bahiya Zahl, Alexa Nino de Rivera, Wellington Muchero, Li Fuxin, Steven H. Strauss
Summary: Adventitious rooting (AR) plays a crucial role in tree propagation, breeding, and genetic engineering. However, the genetic basis of AR is not well-characterized. To identify genetic regulators of AR, a genome-wide association study (GWAS) was performed using 1148 Populus trichocarpa genotypes. A computer vision system was developed to measure various traits related to adventitious root development. GWAS analysis revealed 277 unique associations, indicating that genes involved in hormone signaling, cell division, and other root development processes play a role in AR. Numerous genes with unknown functions were also identified as candidates for further analysis.
HORTICULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Xiaocen Ma, Haobo Yang, Yufen Bu, Xinyuan Wu, Na Sun, Jianwei Xiao, Yanping Jing
Summary: In this study, 18 PtPLATZ genes were identified and classified into five groups in Populus trichocarpa. These genes showed similar exon-intron structures and protein motifs. The PtPLATZ genes were highly responsive to heavy metal stress. Transgenic expression of PtPLATZ3 significantly improved Cd tolerance and accumulation in Populus.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Jun Ni, Ning Zhang, Yang Zhan, Kexin Ding, Peng Qi, Xuejun Wang, Wona Ding, Maojun Xu
Summary: The overexpression of the Ginkgo biloba DFR gene in tobacco plants leads to changes in plant phenotype, including reduced fresh weights, impaired root gravitropic responses, wrinkled leaves, and delayed flowering. The levels of anthocyanins and flavonoid compounds are also altered in the transgenic tobacco plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Menglin Shi, Lei Zhao, Yong Wang
Summary: Photorespiration in Chlamydomonas is more complex than previously thought, with multiple candidate genes encoding hydroxypyruvate reductases identified. These proteins play crucial roles in photorespiration, with different functions in mitochondria and chloroplasts. The findings provide a valuable knowledge base for further studies on the functions of CrHPRs in photorespiration, with potential applications in crop improvement through genetic engineering.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Chaochen Yang, Pengfei Wu, Yongqing Cao, Bingbing Yang, Linxiu Liu, Juanjuan Chen, Renying Zhuo, Xiaohua Yao
Summary: The outbreak of anthracnose caused by Colletotrichum spp. severely affects oil tea production in China. This study reveals the physiological responses and flavonoid-mediated defense mechanisms in disease-resistant C. oleifera. The overexpression of CoDFR increases flavonoid content and enhances anthracnose resistance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jere Vainio, Saku Mattila, Sara M. Abdou, Nina Sipari, Teemu H. Teeri
Summary: Anthocyanins determine the color of flowers, but not all plants can produce all colors. Some plants lack specific enzymes, resulting in the inability to produce blue or orange-red flowers. The substrate specificity of the enzyme is the key to this issue. By introducing mutations to the active site of the enzyme, petunias were able to produce orange flowers and higher amounts of pelargonidin-based anthocyanins.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Forestry
Zhao Aiguo, Ding Ruiwen, Wang Cheng, Chen Cheng, Wang Dongmei
Summary: In this study, the gene encoding DFR from Zanthoxylum bungeanum Maxim. was cloned and analyzed. The results revealed that ZbDFR is a key enzyme involved in the synthesis of anthocyanins and proanthocyanins, and its activity is regulated by flavonoids. Site-directed mutagenesis experiments confirmed the importance of specific amino acid residues in the catalytic and regulatory functions of ZbDFR.
Article
Plant Sciences
Z. Hu, S. Zhang, H. Zhang, L. Cao, R. Chang, Z. Liu, Z. Xu, G. Liu
Summary: This study analyzed the expression patterns of genes encoding CarA and CarB in Populus trichocarpa and investigated their molecular responses under different nitrogen concentrations. The study found that the PtCarA and PtCarB genes are regulated by nitrogen, but their regulatory patterns differ in different tissues. Phylogenetic analysis, expression patterns, and promoter elements suggest the possible coevolution among PtCarA1, PtCarA2, and PtCarB.
Article
Plant Sciences
Nathan Bryant, Jin Zhang, Kai Feng, Mengjun Shu, Raphael Ployet, Jin-Gui Chen, Wellington Muchero, Chang Geun Yoo, Timothy J. Tschaplinski, Yunqiao Pu, Arthur J. Ragauskas
Summary: By characterizing a population of 409 naturally varying Populus trichocarpa genotypes, a genome-wide association study identified 756 genes significantly associated with lignin phenotypes, providing insights into the molecular mechanisms of lignin biosynthesis and new targets for genetic improvement in poplar.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Meng Qi, Shengjie Wang, Na Li, Lingfeng Li, Yue Zhang, Jingyi Xue, Jingyi Wang, Rongling Wu, Na Lian
Summary: Nineteen TPX2 family members were identified and analyzed in the poplar genome, revealing their structural characteristics and gene expression patterns. The TPX2 members showed conserved structural characteristics but exhibited different expression profiles in different tissues, indicating their varying roles in plant growth. Moreover, several cis-acting regulatory elements responsive to light, hormones, and abiotic stress were detected on the promoters of PtTPX2 genes. Additionally, the expression analysis in various tissues of Populus trichocarpa showed that the PtTPX2 genes responded differently to heat, drought, and salt stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Ning Wang, Xiaochun Shu, Fengjiao Zhang, Weibing Zhuang, Tao Wang, Zhong Wang
Summary: The study identified key differentially expressed genes in Lycoris petals during flower development and explored their correlation with anthocyanin content. Through KEGG enrichment analysis, these genes were classified and structural genes and transcription factor genes involved in anthocyanin accumulation were discovered. The important structural gene LrDFR1 showed a positive correlation in expression levels during flower development and acted as a key gene in the anthocyanin metabolism network.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Mengxuan Ren, Yang Zhang, Cong Liu, Yingying Liu, Shuanghui Tian, He Cheng, Huaxin Zhang, Hairong Wei, Zhigang Wei
Summary: In this study, the functional characterization of a Populus trichocarpa GATA TF, PtrGATA12, revealed its important role in wood formation by altering secondary cell wall components and stem strength. PtrGATA12 directly bound to the promoters of master TFs and pathway genes, activating them and exerting a coordinated regulation of SCW components biosynthesis pathways.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Fang He, Yu-Jie Shi, Qian Zhao, Kuang-Ji Zhao, Xing-Lei Cui, Liang-Hua Chen, Han-Bo Yang, Fan Zhang, Jia-Xuan Mi, Jin-Liang Huang, Xue-Qin Wan
Summary: This study conducted a genome-wide analysis of the PPO gene family in Populus, identifying 18 PtrPPO genes and revealing their characteristics through analyses of gene structure, phylogeny, chromosomal localization, gene replication, cis-elements, and expression patterns. The study results indicate that the PtrPPO gene family plays a crucial role in stress responses and may have important implications for stress resistance in forest trees.
Article
Plant Sciences
Wei Sun, Nana Zhou, Yuhan Wang, Shiyu Sun, Yan Zhang, Zhigang Ju, Yin Yi
Summary: Rhododendron delavayi is known for its popular ornamental qualities, particularly its bright red flowers. The newly discovered DFR gene RdDFR1 is crucial in anthocyanin biosynthesis, affecting flower color formation. Genetic manipulation with RdDFR1 in Arabidopsis thaliana and tobacco showed restoration of pigment biosynthesis and altered flower color, highlighting its significance in vivo.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Forestry
Yan Huang, Hong Liu, Zhichun Jia, Qing Fang, Keming Luo
Article
Forestry
Yan Huang, Lili Wang, Shanglian Hu, Xuegang Luo, Ying Cao
TREE GENETICS & GENOMES
(2020)
Article
Environmental Sciences
Yan Huang, Jinlong Lai, Yang Huang, Xuegang Luo, Xu Yang, Zewei Liu, Yue Duan, Chen Li
Summary: This study investigated the effects of nano-BiOI on freshwater microalgae and its toxicological mechanisms. The results showed that nano-BiOI stimulated algal growth at low concentrations but inhibited growth at higher concentrations. Nano-BiOI induced the generation of intracellular reactive oxygen species and interfered with the mineral nutrient metabolism of the algae. Metabolomics analysis revealed significant alterations in metabolic pathways under high concentrations of nano-BiOI.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Yan Huang, Qian Liao, Shanglian Hu, Ying Cao, Gang Xu, Zhijian Long, Xueqin Lu
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
(2018)
