Article
Cell Biology
Yang Lin, Xin-Jun Zhang, Jiajun Yang, Shuo Li, Laura Li, Xiaohui Lv, Jian Ma, Song-Hai Shi
Summary: Sensory neurons in the neocortex play a key role in forming the neural map. The neocortical map of the visual cortex in rodents has a salt-and-pepper pattern, while in higher mammals it is clustered. This study reveals that synaptic connectivity among excitatory neurons, dependent on their kinship, influences the organization of the functional map in the mouse primary visual cortex. The removal of clustered protocadherins disrupts functional selectivity differences between neurons and alters neocortical map organization.
Article
Immunology
Fan Zhang, Fang Hong, Lu Wang, Renjie Fu, Jin Qi, Boyang Yu
Summary: This study elucidated the mechanism of how MrgprX2 regulates intracellular calcium levels in mast cell degranulation through the PI3K/AKT and PLCγ pathways.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biology
Lauren Tereshko, Ya Gao, Brian A. Cary, Gina G. Turrigiano, Piali Sengupta
Summary: Primary cilia play a crucial role in regulating neuronal development and excitability, with recent studies showing their involvement in strengthening synaptic connections and increasing neuronal firing rates. The neuropeptide receptor SSTR3 is predominantly localized in the cilia of excitatory neurons, where it bidirectionally modulates excitatory synaptic inputs. Defects in ciliary neuropeptidergic signaling may contribute to behavioral and cognitive disorders associated with ciliopathies.
Article
Agriculture, Multidisciplinary
Nguyen Minh Trang, Eun-Nam Kim, Thi Hoa Pham, Gil-Saeng Jeong
Summary: Osteoporosis and Alzheimer's disease are common types of dementia in seniors, and they share common risk factors. Previous studies have shown that individuals with osteoporosis are more susceptible to Alzheimer's disease. Citropten, a compound found in Citrus aurantifolia, has various pharmacological activities, and this study reveals its potential in suppressing osteoclastogenesis through Aβ regulation. The findings suggest that citropten is a potential candidate for treating osteoclastogenesis-related diseases.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zhihua Ma, Yi Zeng, Ming Wang, Wei Liu, Jiafeng Zhou, Chao Wu, Lin Hou, Bin Yin, Boqin Qiang, Pengcheng Shu, Xiaozhong Peng
Summary: In this study, the negative modulator N4BP1 in neural progenitor cells was found to regulate the dynamics of Notch signaling pathway by mediating the stability of NICD after Notch1 S3 cleavage. The CoCUN domain in N4BP1, particularly the Phe-Pro motif, played a crucial role in mediating the degradation of NICD. Additionally, the E3 ligase Trim21 was specifically required for N4BP1-regulated NICD degradation. Overexpression of N4BP1 promoted neural stem cell differentiation, while the absence of N4BP1 sensitized neural progenitor cells to Notch signaling, resulting in the maintenance of stem-like properties and reduced production of cortical neurons in the cerebral cortex.
Article
Neurosciences
Hemalatha Muralidharan, Shrobona Guha, Kiran Madugula, Ankita Patil, Sarah A. Bennison, Xiaohuan Sun, Kazuhito Toyo-oka, Peter W. Baas
Summary: This study reveals the essential role of KIFC1 in ensuring the accuracy of neuronal migration trajectory. By crosslinking microtubules into a nonsliding mode, KIFC1 enables dynein-driven forces to achieve sufficient traction and thus allows neurons to migrate along their appropriate path.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Beatrice Paola Festa, Farah H. Siddiqi, Maria Jimenez-Sanchez, Hyeran Won, Matea Rob, Alvin Djajadikerta, Eleanna Stamatakou, David C. Rubinsztein
Summary: In neurodegenerative diseases, activated microglia secrete pro-inflammatory factors, disrupting neuronal autophagy and protein clearance. The microglia-derived CCL-3/-4/-5 activate neuronal CCR5, leading to mTORC1 activation and autophagy dysfunction. Upregulation of CCR5 and its chemokines in mouse models suggests their role in the early phases of Huntington's disease (HD) and tauopathy. Inhibiting CCR5 can rescue autophagy dysfunction and improve HD and tau pathologies in mouse models.
Article
Cell Biology
Menglin Sha, Hongxing Li, Bingyan Guo, Xiaoyong Geng
Summary: The myeloid-specific knockout of SHP2 has been found to improve cardiac function, reduce infarct size, and decrease apoptosis and inflammatory cell infiltration in a mouse model of myocardial infarction. Furthermore, it mediates the PI3K/PLC gamma signaling pathway in macrophages, enhancing their endocytosis and reducing the expression of inflammatory cytokines.
Article
Multidisciplinary Sciences
Mihoko Kato, Irina Kolotuev, Alexandre Cunha, Shahla Gharib, Paul W. Sternberg
Summary: This study reveals the function of muscarinic receptor GAR-3 in epithelial cell migration in C. elegans, including receiving signals from cholinergic neurons, affecting migratory path, and determining migration direction. The experimental results suggest that the regulation of GAR-3 receptor in LC is accomplished by specific downstream signaling pathways, and there is a clear correlation between GAR-3 receptor activation level and LC migration direction.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Alessandro Bortolami, Wei Yu, Elena Forzisi, Koray Ercan, Ritik Kadakia, Madhuvika Murugan, Denise Fedele, Irving Estevez, Detlev Boison, Mladen-Roko Rasin, Federico Sesti
Summary: The study investigates the role of voltage-gated potassium channels in neocortical development. A genetic mutation in these channels can lead to severe neuromorphological abnormalities, but can be rescued through pharmacological intervention. This research reveals the importance of developmental channelopathies in the etiology of certain brain disorders.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Oncology
Yuqi Li, Rongrong Li, Xu Luo, Fang Xu, Meizi Yang, Lanhui Zheng, Qihao Wu, Wenguo Jiang, Yana Li
Summary: This study investigated the effect of vascular endothelial growth factor B (VEGFB) on regulating insulin secretion in type 2 diabetes mellitus (T2DM) and its underlying mechanism. The results showed that knockout of VEGFB exacerbated the decrease in insulin secretion in T2DM mice, while overexpression of VEGFB improved glucose tolerance and insulin sensitivity. VEGFB activated the Ca2+/CaMK2 pathway via VEGFR1-PLC gamma and IP3R pathway to regulate insulin secretion in T2DM.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Biology
Amit Jairaman, Amanda McQuade, Alberto Granzotto, You Jung Kang, Jean Paul Chadarevian, Sunil Gandhi, Ian Parker, Ian Smith, Hansang Cho, Stefano L. Sensi, Shivashankar Othy, Mathew Blurton-Jones, Michael D. Cahalan
Summary: The membrane protein TREM2 plays a crucial role in regulating key functions of microglial cells, such as calcium signaling, phagocytosis, and chemotaxis. Loss of TREM2 leads to exaggerated calcium signaling in response to purinergic agonists, resulting in altered cell motility and chemotaxis.
Article
Neurosciences
Rishav Mitra, Gaiti Hasan
Summary: Recent studies have shown that store-operated Ca2+ entry plays an important role in regulating neuronal function in Drosophila and mouse, with changes in ion channel function observed in neurons when this entry is lost. These changes are specific to neuronal subtypes. Gene expression studies in the mouse brain have also revealed differences in the expression of genes involved in ER-store Ca2+ release and store-operated Ca2+ entry across different neuronal classes. Loss of store-operated Ca2+ entry in neurons affects neuronal gene expression profiles, including genes encoding ion channels. Further research is needed to understand the functional significance of store-operated Ca2+ entry in specific neuronal subtypes and in the context of neurodegenerative syndromes.
CURRENT OPINION IN NEUROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kana Aoki, Shota Harada, Keita Kawaji, Kenji Matsuzawa, Seiichi Uchida, Junichi Ikenouchi
Summary: The cytoplasm in mammalian cells is considered homogeneous. Here authors report that the cytoplasmic fluidity is regulated in the blebbing cells, which is regulated by calcium concentration in the expanding blebs and involves the STIM-Orai1 pathway.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Mohammad A. Ghane, Wei Wei, Dina W. Yakout, Zachary D. Allen, Cassandra L. Miller, Bin Dong, Jenny J. Yang, Ning Fang, Angela M. Mabb
Summary: The study found that disrupting Arc protein ubiquitination enhances DHPG-induced ER-mediated Ca2+ release. This alteration is observed in all neuronal subregions except secondary branchpoints. Disruptions in Arc ubiquitination increase Arc interaction with the integral ER protein Calnexin.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Lauri M. Louhivuori, Pauli M. Turunen, Verna Louhivuori, Venkatram Yellapragada, Tommy Nordstrom, Per Uhlen, Karl E. Akerman
Article
Biochemistry & Molecular Biology
Thomas Liebmann, Nicolas Fritz, Markus Kruusmaegi, Linda Westin, Kristoffer Bernhem, Alexander Bondar, Anita Aperia, Hjalmar Brismar
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Biochemical Research Methods
Ivar Dehnisch Ellstrom, Stefan Spulber, Sara Hultin, Nils Norlin, Sandra Ceccatelli, Claes Hultling, Per Uhlen
JOURNAL OF NEUROSCIENCE METHODS
(2019)
Article
Oncology
Cristian Ibarra, Marie Karlsson, Simone Codeluppi, Manuel Varas-Godoy, Songbai Zhang, Lauri Louhivuori, Sara Mangsbo, Arad Hosseini, Navid Soltani, Rahim Kaba, T. Kalle Lundgren, Abolfazl Hosseini, Nobuyuki Tanaka, Mototsugu Oya, Peter Wiklund, Ayako Miyakawa, Per Uhlen
MOLECULAR ONCOLOGY
(2019)
Article
Neurosciences
Paola Rebellato, Agmara Kaczynska, Shigeaki Kanatani, Ibrahim Al Rayyes, Songbai Zhang, Carlos Villaescusa, Anna Falk, Ernest Arenas, Ola Hermanson, Lauri Louhivuori, Per Uhlen
Article
Urology & Nephrology
Alexandra Grahn, Nobuyuki Tanaka, Per Uhlen, Marianne Brehmer
WORLD JOURNAL OF UROLOGY
(2019)
Article
Biochemistry & Molecular Biology
Margit Kaldmae, Axel Leppert, Gefei Chen, Medoune Sarr, Cagla Sahin, Kerstin Nordling, Nina Kronqvist, Marta Gonzalvo-Ulla, Nicolas Fritz, Axel Abelein, Sonia Lain, Henrik Biverstal, Hans Jornvall, David P. Lane, Anna Rising, Jan Johansson, Michael Landreh
Article
Multidisciplinary Sciences
Erik Smedler, Lauri Louhivuori, Roman A. Romanov, Debora Masini, Ivar Dehnisch Ellstrom, Chungliang Wang, Martino Caramia, Zoe West, Songbai Zhang, Paola Rebellato, Seth Malmersjo, Irene Brusini, Shigeaki Kanatani, Gilberto Fisone, Tibor Harkany, Per Uhlen
Summary: This study demonstrates that embryonic deletion of CACNA1C in neurons destined for the cerebral cortex disrupts Ca2+ activity, leading to abnormal brain development and anxiety.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Biochemical Research Methods
Shigeaki Kanatani, Per Uhlen
Article
Multidisciplinary Sciences
Matthew D. Lee, Charlotte Buckley, Xun Zhang, Lauri Louhivuori, Per Uhlen, Calum Wilson, John G. McCarron
Summary: The endothelial network, composed of specialized endothelial cells, plays a crucial role in maintaining vascular function. By utilizing short path lengths and rapid signal transmission, the endothelial cells are able to communicate efficiently, allowing them to regulate vascular activity effectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Songbai Zhang, Ayako Miyakawa, Malin Wickstrom, Cecilia Dyberg, Lauri Louhivuori, Manuel Varas-Godoy, Kati Kemppainen, Shigeaki Kanatani, Dagmara Kaczynska, Ivar Dehnisch Ellstrom, Lotta Elfman, Pauliina Kronqvist, Heli Repo, Katsuhiko Mikoshiba, Cecilia Sahlgren, John Inge Johnsen, Per Uhlen
Summary: This study reveals the negative regulation role of G protein-coupled receptor kinase-interacting protein 1 (GIT1) in the Notch signaling pathway and tumor growth in estrogen receptor-negative (ER-) breast cancer. GIT1 inhibits the translocation of Notch ICD to the nucleus, thus modulating the Notch signaling and acting as a guardian against breast cancer growth.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Shanzheng Yang, Enrique M. Toledo, Pedro Rosmaninho, Changgeng Peng, Per Uhlen, Diogo S. Castro, Ernest Arenas
COMMUNICATIONS BIOLOGY
(2018)
Article
Oncology
Mia Niklasson, Gianluca Maddalo, Zuzana Sramkova, Ercan Mutlu, Shimei Wee, Petra Sekyrova, Linnea Schmidt, Nicolas Fritz, Ivar Dehnisch, Gregorios Kyriatzis, Michaela Krafcikova, Brittany B. Carson, Jennifer M. Feenstra, Voichita D. Marinescu, Anna Segerman, Martin Haraldsson, Anna-Lena Gustavsson, Lars G. J. Hammarstrom, Annika Jenmalm Jensen, Lene Uhrbom, A. F. Maarten Altelaar, Sten Linnarsson, Per Uhlen, Lukas Trantirek, C. Theresa Vincent, Sven Nelander, Per Oyvind Enger, Michael Andang
Article
Health Care Sciences & Services
Andreas Alga, Sidney Wong, Rawand Haweizy, Kalle Conneryd Lundgren, Johan von Schreeb, Jonas Malmstedt
JMIR RESEARCH PROTOCOLS
(2018)