Article
Multidisciplinary Sciences
Ryo Horikoshi, Kimihiko Goto, Masaaki Mitomi, Kazuhiko Oyama, Tomoyasu Hirose, Toshiaki Sunazuka, Satoshi Omura
Summary: Afidopyropen is a novel insecticide with strong insecticidal activity against various agricultural pests, while demonstrating safety profiles towards non-target organisms.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Ravi R. Sonani, Artur Blat, Grzegorz Dubin
Summary: This study solved the crystal structures of human ACOX1a, revealing its homodimeric form and the binding of two FAD molecules at the active site. It also found that the substrate binding cleft of ACOX1a is wider compared to other fatty acid oxidation enzymes. Furthermore, the study analyzed the impact of mutations causing dysfunctionality of ACOX1a on its structure and function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Pharmacology & Pharmacy
Hiroki Hashizume, Tatsuki Fukami, Kanji Mishima, Hiroshi Arakawa, Kenji Mishiro, Yongjie Zhang, Masataka Nakano, Miki Nakajima
Summary: This study aimed to clarify the human ACS isoforms responsible for CoA-conjugation of NSAIDs by considering the hepatic expression levels of ACS isoforms. Among the 10 types of NSAIDs studied, propionic acid-class NSAIDs were found to be conjugated with CoA in the human liver, while NSAIDs in other classes did not exhibit this reaction. ACSL1 was identified as the most highly expressed ACS isoform in the human liver, and could catalyze the CoA conjugation of propionic acid-class NSAIDs, which may lead to toxicity through protein adduct formation.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jingjing Sun, Congyu Yao, Yujie Li, Wei Wang, Jianhua Hao, Yi Yu
Summary: In this study, a novel β-galactosidase was isolated from a deep-sea metagenome, overexpressed in Escherichia coli, and purified. The enzyme showed high activity at specific temperatures and pH conditions, and exhibited resistance to salt. Moreover, it displayed hydrolysis and transglycosylation activity towards substrates such as oNPG and lactose.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Weibo Wang, Qingpeng Wei, Jiayuan Zhang, Meiqi Zhang, Chuchen Wang, Renyu Qu, Yuan Wang, Guangfu Yang, Jing Wang
Summary: This study developed a genetically encoded fluorescent sensor for ratiometric quantification of LCACoAs in living cells, demonstrating conformational changes of LCACoA molecules and real-time monitoring. The research also revealed the impact of disruptions in ACSL enzymes and ACBP genes on the levels and distribution of LCACoAs in cytosol and mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biotechnology & Applied Microbiology
Yi-De Liu, Ge Yuan, Yu-Ting An, Zi-Ran Zhu, Gang Li
Summary: A rare bifunctional cellobiohydrolase/beta-xylosidase Cbh2124 was identified from a metagenomic library of mangrove soil samples, showing high catalytic efficiency and thermostability.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Sylwia Szrok-Jurga, Aleksandra Czumaj, Jacek Turyn, Areta Hebanowska, Julian Swierczynski, Tomasz Sledzinski, Ewa Stelmanska
Summary: Fatty acid metabolism, especially beta-oxidation, plays a crucial role in human physiology and pathology. Beta-oxidation is not only involved in energy metabolism but also contributes to the synthesis of various important compounds. This review provides an overview of the importance of mitochondrial and peroxisomal beta-oxidation in different organs and cells and discusses the diseases associated with fatty acid oxidation disturbances. The review also highlights the abnormalities of fatty acid oxidation in cancer cells and diseases caused by mutations in enzymes involved in fatty acid oxidation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Haidong Peng, Yaya Wang, Kai Jiang, Xinru Chen, Wenlu Zhang, Yanan Zhang, Zixin Deng, Xudong Qu
Summary: This study identified a crucial biosynthetic pathway that efficiently converts phytosterols into 4-Androstenedione and progesterone, providing effective production routes for steroid drugs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Isha Kashyap, Rachayeeta Deb, Abhigna Battineni, Shirisha Nagotu
Summary: Beta-oxidation is an important metabolic pathway that is shared by mitochondria and peroxisomes in mammalian cells. In yeast, peroxisomes are the only site for fatty acid degradation. The enzyme acyl CoA oxidase, located in the peroxisomal matrix, catalyzes the first reaction of this pathway. Studies have shown the conserved function of this protein in fatty acid oxidation across different organisms. Its importance is demonstrated by the lethal conditions caused by altered function in humans. This review discusses various aspects, including gene expression, structure, folding, and import of the protein in both yeast and human cells, as well as recent findings on its role in human health, aging, and associated mutations in patients.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Environmental Sciences
Shohreh Ariaeenejad, Kaveh Kavousi, Behrouz Zolfaghari, Swapnoneel Roy, Takeshi Koshiba, Ghasem Hosseini Salekdeh
Summary: A large amount of lignocellulosic waste is generated every day, which has severe environmental pollution effects. Using enzymes as biocatalysts for the degradation of lignocellulosic materials is a practical step towards green energy and environmental biosafety. The current study focuses on enzyme computationally screened from camel rumen metagenomics data with the capacity to degrade lignocellulosic-rich and recalcitrant materials.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Runyi Huang, Wenli Yu, Rongzhen Zhang, Yan Xu
Summary: This study developed an efficient method for producing malonyl-CoA by screening the ACS gene from Streptomyces sp. The newly characterized ACS enzyme showed high conversion rate and yield of malonyl-CoA under optimized conditions.
PROCESS BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Chong-Yang Ren, Yong Liu, Wen-Ping Wei, Junbiao Dai, Bang-Ce Ye
Summary: This study created a valuable heterologous host Ab Delta ery by knocking out the erythromycin biosynthesis gene cluster, which showed higher production of heterologous products than the wild type strain.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zool Hilmi Ibrahim, Jung-Hoon Bae, Bong Hyun Sung, Mi-Jin Kim, Ahmad Hazri Ab Rashid, Jung-Hoon Sohn
Summary: This study investigated the functions and substrate specificities of different isozymes of CaAOX proteins. It was found that CaAOX2 isozyme is specific for short- and medium-chain fatty acids, while CaAOX5 isozyme prefers long-chain fatty acids. CaAOX4 isozyme has a broad substrate spectrum. These findings highlight the importance of CaAOX4 for the complete beta-oxidation of long-chain fatty acids.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tao Wang, Hao Shi, William B. Whitman
Summary: DmdC1 from R. pomeroyi shows high affinity for short-chain acyl-CoAs in addition to its natural substrate MMPA-CoA, and is not affected by potential effectors tested, suggesting minimal adaptations to DMSP metabolism and recent evolution from a short-chain acyl-CoA dehydrogenase involved in fatty acid oxidation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Editorial Material
Clinical Neurology
Brandon B. Holmes, Jeffrey B. Russ, Yvonne W. Wu, Renata C. Gallagher, Dawn Gano
Summary: The newborn male infant was born prematurely at 36 weeks, with low birth weight, but he behaved normally and breastfed well in the first 40 hours of life.
Article
Chemistry, Physical
Chloe M. Lelievre, Melanie Balandras, Jean-Louis Petit, Carine Vergne-Vaxelaire, Anne Zaparucha
Article
Chemistry, Applied
Adam A. Caparco, Eric Pelletier, Jean Louis Petit, Aurelie Jouenne, Bettina R. Bommarius, Veronique de Berardinis, Anne Zaparucha, Julie A. Champion, Andreas S. Bommarius, Carine Vergne-Vaxelaire
ADVANCED SYNTHESIS & CATALYSIS
(2020)
Article
Oncology
Trisha Dwight, Edward Kim, Karine Bastard, Diana E. Benn, Graeme Eisenhofer, Susan Richter, Massimo Mannelli, Elena Rapizzi, Aleksander Prejbisz, Mariola Peczkowska, Karel Pacak, Roderick Clifton-Bligh
Summary: Somatic mutations in EPAS1 are associated with various phenotypes, while the pathogenic potential of germline variants remains unclear. Functional studies showed that three germline variants of EPAS1 have similarities to somatic activating mutations in PPGL, suggesting they may act as modifiers in the expression of PPGL.
ENDOCRINE-RELATED CANCER
(2021)
Article
Biochemical Research Methods
Ekaterina Darii, Yves Gimbert, Sandra Alves, Annelaure Damont, Alain Perret, Amina S. Woods, Francois Fenaille, Jean-Claude Tabet
Summary: Mass spectrometric investigations revealed the formation of stable sodiated noncovalent complex anions between fructose and arginine. Unexpected deuterium labeling and hydrogen/deuterium exchange phenomena were observed, indicating zwitterionic structures solvated by a single water molecule. Back hydrogen/deuterium exchange impact on deuterium depletion was illustrated, showing complementarity in partner exchange and selective back exchange validation of salt-solvated structures.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Chalore Teepakorn, Petra Zajkoska, Gregory Cwicklinski, Veronique De Berardinis, Anne Zaparucha, Guillaume Nonglaton, Zoe Anxionnaz-Minvielle
Summary: In this study, enzyme immobilization methods and a collection of diverse nitrilases were investigated for the conversion of 3-cyanopyridine to nicotinic acid. The best immobilization conditions were achieved through cross-linking with glutaraldehyde, showing good activity in a batch reactor over multiple cycles. Utilizing a new thermostable nitrilase in a continuous packed bed reactor resulted in complete conversion of 3-cyanopyridine over 30 days of operation, demonstrating high stability of the biocatalytic process.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Microbiology
Madeleine Bouzon, Volker Doring, Ivan Dubois, Anne Berger, Gabriele M. M. Stoffel, Liliana Calzadiaz Ramirez, Sophia N. Meyer, Marion Foure, David Roche, Alain Perret, Tobias J. Erb, Arren Bar-Even, Steffen N. Lindner
Summary: This study explores the potential and limits of evolving enzyme cofactor specificity within the cellular context. By using adaptive evolution experiments in an NADPH-auxotrophic strain of E. coli, mutations were found in malic enzyme and dihydrolipoamide dehydrogenase that altered their cofactor preference. However, the study also reveals that biochemical constraints, such as unfavorable thermodynamics, limit the evolution of central metabolism oxidoreductases toward reducing NADP(+).
Article
Biotechnology & Applied Microbiology
Thomas Bessonnet, Aline Mariage, Jean-Louis Petit, Virginie Pellouin, Adrien Debard, Anne Zaparucha, Carine Vergne-Vaxelaire, Veronique de Berardinis
Summary: The study identified a thermostable nitrilase Nit(phym) from Paraburkholderia phymatum, which exhibited high operational stability, thermostability, and a broad pH range. This nitrilase also displayed high resistance and tolerance, enabling efficient conversion of various nitriles.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Pierre Joly, Alexandra Calteau, Aurelie Wauquier, Remi Dumas, Mylene Beuvin, David Vallenet, Julien Crovadore, Bastien Cochard, Francois Lefort, Jean-Yves Berthon
Summary: The study analyzed the B25 strain, a promising plant growth promoting rhizobacterium and inhibitor of pathogenic fungi, which showed no antibiotic resistance genes and demonstrated efficiency against various pathogens. With its good properties for industrialization and environmental-friendly profile, B25 has been commercialized under market authorization since 2018.
Article
Chemistry, Physical
Ewald P. J. Jongkind, Aurelie Fossey-Jouenne, Ombeline Mayol, Anne Zaparucha, Carine Vergne-Vaxelaire, Caroline E. Paul
Summary: Accessing chiral amines with high enantiomeric and diastereomeric excess was achieved through the development of a bi-enzymatic cascade using Old Yellow Enzyme family enzymes. Different unsaturated carbonyl substrates were successfully converted with up to 99% selectivity, showcasing the potential of this approach for diverse alkene substrates.
Article
Biology
Steff Horemans, Matthaios Pitoulias, Alexandria Holland, Emilie Pateau, Christophe Lechaplais, Dariy Ekaterina, Alain Perret, Panos Soultanas, Laurent Janniere
Summary: The study shows that PykA in Bacillus subtilis plays a crucial role in DNA replication, with PEPut acting as an initiation inhibitor and Cat as a replication fork speed activator. PykA modulates the activities of essential enzymes for replication initiation and elongation. PEPut regulates PykA activity, and determinants of Cat and PEPut are important for PykA's replication functions.
Article
Biochemistry & Molecular Biology
Nadia Perchat, Christelle Dubois, Remi Mor-Gautier, Sophie Duquesne, Christophe Lechaplais, David Roche, Stephanie Fouteau, Ekaterina Darii, Alain Perret
Summary: Bacteria can modify their phenotype through efficient utilization of secondary substrates by promiscuous enzymes in a novel metabolic pathway. This pathway appears to be widespread among prokaryotes, as indicated by the presence of conserved genes in hundreds of genomes across multiple phyla.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Valerie A. Delmas, Nadia Perchat, Oriane Monet, Marion Foure, Ekatarina Darii, David Roche, Ivan Dubois, Emilie Pateau, Alain Perret, Volker Doring, Madeleine Bouzon
Summary: This study successfully implemented formate assimilation in Escherichia coli through stepwise adaptive evolution, revealing the mutational path and crucial enzymatic variations in the synthetic pathway. The results provide important theoretical basis for pathway engineering.
METABOLIC ENGINEERING
(2022)
Article
Chemistry, Physical
Laurine Ducrot, Megan Bennett, Gwenaelle Andre-Leroux, Eddy Elisee, Sacha Marynberg, Aurelie Fossey-Jouenne, Anne Zaparucha, Gideon Grogan, Carine Vergne-Vaxelaire
Summary: Inspired by the diversity of residues, mutations F140A and W145A were introduced in CfusAmDH, leading to improved conversion and enantioselectivity for certain substrates. The mutant CfusAmDH-W145A showed synthetic potential and crystal structure analysis provided insights into cofactor and substrate specificity, as well as structural dynamics of this enzyme family.
Review
Biotechnology & Applied Microbiology
Erhard Bremer, Alexandra Calteau, Antoine Danchin, Colin Harwood, John D. Helmann, Claudine Medigue, Bernhard O. Palsson, Agnieszka Sekowska, David Vallenet, Abril Zuniga, Cristal Zuniga
Summary: The majority of genomic sequences are automatically annotated, but depend on manual annotation efforts using verified experimental data. This article summarizes the updated functional annotation of Bacillus subtilis strain 168, highlighting new metabolic insights, the role of metals in metabolism and biosynthesis, functions related to biofilm formation, features controlling cell growth, and protein agents allowing class discrimination. The updated sequence, including new genomic objects and literature review, is available at the International Nucleotide Sequence Database Collaboration (INSDC: AccNum AL009126.4).
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Microbiology
Shi-Zhen Wang, Corinne Cruaud, Jean-Marc Aury, David Vallenet, Julie Poulain, Benoit Vacherie, Anne Zaparucha, Carine Vergne-Vaxelaire
Summary: Pseudomonas marincola YsY11 and Pseudomonas oleovorans T9AD were isolated from marine environments in the Pacific Ocean, and their whole-genome sequences were reported. YsY11 has a single 4.77 Mb chromosome, while T9AD has a 5.57 Mb chromosome and a 2.8 kb plasmid.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)