Journal
PLOS ONE
Volume 6, Issue 9, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0024571
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Categories
Funding
- Asthma U.K.
- Biotechnology and Biological Sciences Research Council [BB/H019634/1]
- National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, USA
- UK Department of Health via the National Institute for Health Research (NIHR) Comprehensive Biomedical Research Centre
- King's College London
- Research Councils U.K.
- BBSRC [BB/H019634/1] Funding Source: UKRI
- MRC [G0400106, G0501494] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H019634/1] Funding Source: researchfish
- Medical Research Council [G1000758, G0400106, G0501494, G1000758B] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0508-10212] Funding Source: researchfish
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Antibodies are assembled by a highly orchestrated series of recombination events during B cell development. One of these events, class switch recombination, is required to produce the IgG, IgE and IgA antibody isotypes characteristic of a secondary immune response. The action of the enzyme activation induced cytidine deaminase is now known to be essential for the initiation of this recombination event. Previous studies have demonstrated that the immunoglobulin switch regions acquire distinct histone modifications prior to recombination. We now present a high resolution analysis of these histone modifications across the IgE switch region prior to the initiation of class switch recombination in primary human B cells and the human CL-01 B cell line. These data show that upon stimulation with IL-4 and an anti-CD40 antibody that mimics T cell help, the nucleosomes of the switch regions are highly modified on histone H3, accumulating acetylation marks and tri-methylation of lysine 4. Distinct peaks of modified histones are found across the switch region, most notably at the 5' splice donor site of the germline (I) exon, which also accumulates AID. These data suggest that acetylation and K4 tri-methylation of histone H3 may represent marks of recombinationally active chromatin and further implicates splicing in the regulation of AID action.
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