Article
Microbiology
Tamara Alhamami, Piklu Roy Chowdhury, Nancy Gomes, Mandi Carr, Tania Veltman, Manouchehr Khazandi, Joanne Mollinger, Ania T. Deutscher, Conny Turni, Layla Mahdi, Henrietta Venter, Sam Abraham, Steven P. Djordjevic, Darren J. Trott
Summary: The study identified varying levels of antimicrobial resistance in Manheimia haemolytica and Pasteurella multocoda isolates from BRD cases in Australia, confirming the first emergence of AMR. Close monitoring of the situation is warranted.
Article
Agriculture, Dairy & Animal Science
Johan Manuel Calderon Bernal, Ana Fernandez, Jose Luis Arnal, Celia Sanz Tejero, Jose Francisco Fernandez-Garayzabal, Ana Vela, Dolores Cid
Summary: This study characterized 170 P. multocida isolates from 125 BRD outbreaks using different typing approaches. The results revealed a very low genetic diversity among P. multocida, with most isolates belonging to the A:L3 genotype. Furthermore, the results of MLST and PFGE supported the clonal population structure of BRD-associated P. multocida isolates. These findings highlight the genetic relatedness of most P. multocida isolates associated with BRD in cattle.
Article
Microbiology
Sarah M. Raabis, Chelsea L. Holschbach, Joseph H. Skarlupka, Garret Suen, Theresa L. Ollivett
Summary: This study investigated the relationship between changes in respiratory microbiota and respiratory diseases in dairy calves. The researchers found that increased species abundance in the pre-infection samples was associated with a decreased incidence of respiratory disease. They also evaluated the impact of antibiotic therapy on the microbiota but found no significant effect. The study suggests a potential link between the gut microbiota and respiratory microbiota in dairy calves.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Xue-Song Li, Yu Qi, Jun-Ze Xue, Guan-Yi Xu, Yu-Xuan Xu, Xuan-Yu Li, Inam Muhammad, Ling-Cong Kong, Hong-Xia Ma
Summary: The study found that Pasteurella multocida, a major pathogen of bovine respiratory disease, showed high resistance to the commonly used antibiotic enrofloxacin. Transcriptome sequencing revealed that the expression of the satP gene changed significantly with increasing drug resistance. Further experiments confirmed that the satP gene is related to the resistance and pathogenicity of Pasteurella multocida and may serve as a target for synergistic effects with enrofloxacin.
VETERINARY SCIENCES
(2023)
Article
Plant Sciences
Doris Bismarck, Jens Becker, Elisabeth Muller, Vera Becher, Lisa Nau, Philipp Mayer
Summary: This study investigated the antimicrobial activity of 11 essential oils against Pasteurella multocida, Mannheimia haemolytica, and bacteria in the Mannheimia clade. Results showed that cinnamon cassia and lemongrass oil exhibited strong inhibitory effects on P. multocida, while cinnamon cassia, thyme, wintergreen, lemongrass, and winter savory oil showed strong antimicrobial activity against M. haemolytica. The antimicrobial activity of essential oil mixtures was not stronger than single essential oils.
Article
Microbiology
Hang Xiao, Qin Zhao, Jianlin Yuan, Wei Liang, Rui Wu, Yiping Wen, Senyan Du, Yiping Wang, Shan Zhao, Yifei Lang, Qigui Yan, Xiaobo Huang, Sanjie Cao
Summary: In this study, it was found that IFN-γ plays an important role in regulating Pasteurella multocida toxin-associated pneumonia. IFN-γ deficiency alleviates the severity of pneumonia, increases the survival rate, and reduces lung pathology. Additionally, IFN-γ deficiency decreases myeloperoxidase expression and impairs PANoptosis activation markers, including caspase 3, GSDMD, and MLKL, as well as reduces IL-1 beta expression.
VETERINARY MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Youserya M. Hashem, Walid S. Mousa, Eman E. Abdeen, Hanaa M. Abdelkhalek, Mohammed Nooruzzaman, Ahmad El-Askary, Khadiga A. Ismail, Ayman M. Megahed, Ahmed Abdeen, Enas A. Soliman, Gamal Wareth
Summary: Respiratory infections in young calves in Menoufiya Governorate, Egypt, are primarily caused by Mycoplasma bovis, Mycoplasma bovigenitalium, Pasteurella multocida, and Staphylococcus aureus. Despite a low prevalence in this study compared to others in Egypt, mixed infection was a common occurrence. Genetic characterization revealed the potential spread of these bacterial pathogens through animal contact and trades across different geographic locations worldwide.
Article
Multidisciplinary Sciences
Kimberley J. Hockings, Benjamin Mubemba, Charlotte Avanzi, Kamilla Pleh, Ariane Dux, Elena Bersacola, Joana Bessa, Marina Ramon, Sonja Metzger, Livia V. Patrono, Jenny E. Jaffe, Andrej Benjak, Camille Bonneaud, Philippe Busso, Emmanuel Couacy-Hymann, Moussa Gado, Sebastien Gagneux, Roch C. Johnson, Mamoudou Kodio, Joshua Lynton-Jenkins, Irina Morozova, Kerstin Matz-Rensing, Aissa Regalla, Abilio R. Said, Verena J. Schuenemann, Samba O. Sow, John S. Spencer, Markus Ulrich, Hyacinthe Zoubi, Stewart T. Cole, Roman M. Wittig, Sebastien Calvignac-Spencer, Fabian H. Leendertz
Summary: This study found leprosy-like lesions in wild populations of western chimpanzees in Guinea-Bissau and Cote d'Ivoire, caused by Mycobacterium leprae. The chimpanzee strains were identified as belonging to rare genotypes, suggesting leprosy may be circulating in more wild animals than previously suspected.
Article
Microbiology
Tamara Alhamami, Piklu Roy Chowdhury, Henrietta Venter, Tania Veltman, Alec Truswell, Sam Abraham, Sylvia A. Sapula, Mandi Carr, Steven P. Djordjevic, Darren J. Trott
Summary: Pasteurella multocida isolates from Australian feedlot cattle with bovine respiratory disease exhibited significant genetic diversity, with different capsular serogroups, lipopolysaccharide genotypes, and sequence types. Additionally, antibiotic resistance was predominantly observed in certain sequence types. The study provides insights into the genomic diversity and antimicrobial resistance associations of P. multocida in Australia.
VETERINARY MICROBIOLOGY
(2023)
Article
Infectious Diseases
Alexander Melchner, Sarah van de Berg, Nelly Scuda, Andrea Feuerstein, Matthias Hanczaruk, Magdalena Schumacher, Reinhard K. Straubinger, Durdica Marosevic, Julia M. Riehm
Summary: The study found widespread antimicrobial resistance in bacteria isolated from calves, cattle, and dairy cows in Bavaria, Germany, with a significant increase in multidrug-resistant strains. The proportion of resistant bacteria was significantly higher in farms with larger herd sizes and in fattening farms compared to dairy farms.
Article
Microbiology
Nonzee Hanchanachai, Pramote Chumnanpuen, Teerasak E-kobon
Summary: The study revealed that negative interactions, involving competition for nutrients and space, were the predominant interactions between porcine P. multocida and other bacteria. Analysis of critical molecules in conditioned media may offer a potential approach for controlling the growth of P. multocida.
Article
Microbiology
Thomas R. Smallman, Galain C. Williams, Marina Harper, John D. Boyce
Summary: This study identified essential genes for in vitro growth of P. multocida and genes required for hyaluronic acid capsule production and regulation using transposon-directed insertion site sequencing (TraDIS) and TraDISort. It found that many capsule-associated genes were involved in the stringent response, and disruption of the autoregulatory domains in SpoT resulted in reduced capsule biosynthesis and an acapsular phenotype.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Rezheen F. Abdulrahman, Robert L. Davies
Summary: Diverse temperate bacteriophages, including both Siphoviridae and Myoviridae family-types, were found in Pasteurella multocida isolates from different domesticated animal species, potentially playing key roles in the evolution and virulence of this pathogen. Some bacterial hosts were found to harbor multiple prophages, indicating a diverse phage spectrum. Analysis of phage DNA revealed that closely-related strains may have phages with identical restriction endonuclease types, while others may have acquired phages with different types recently.
Article
Biotechnology & Applied Microbiology
Emily L. Wynn, Michael L. Clawson
Summary: Common bacterial causes of bovine respiratory disease (BRD) include Histophilus somni, Mannheimia haemolytica, and Pasteurella multocida. This study focused on the core genomes and outer membrane proteins (OMPs) of these bacteria, providing potential candidates for further research and interventions against BRD.
Article
Veterinary Sciences
Xingping Wu, Zheng Zeng, Hongliang Tian, Lianci Peng, Dongyi Xu, Yu Wang, Chao Ye, Yuanyi Peng, Rendong Fang
Summary: In this study, the mechanism of P. multocida-induced NLRP6 inflammasome activation was investigated. It was found that Nlrp6 (-/-) mice were more susceptible to P. multocida infection and had higher bacterial burden in the lungs. NLRP6 was shown to regulate inflammatory cytokine secretion and mediate caspase-1 activation and ASC oligomerization, resulting in IL-1β secretion.
VETERINARY RESEARCH
(2022)
Article
Microbiology
Karen Schlez, Tobias Eisenberg, Joerg Rau, Sabine Dubielzig, Matthias Kornmayer, Georg Wolf, Anja Berger, Alexandra Dangel, Christiane Hoffmann, Christa Ewers, Andreas Sing
Summary: Corynebacterium diphtheriae, a significant pathogen causing human diphtheria, has recently been found to include novel species such as C. belfantii and C. rouxii. This study reported rare cases of C. rouxii isolation from dogs with multiple bacterial infections, suggesting that skin infections in companion animals may be caused by human-specific pathogens, resembling cutaneous diphtheria clinically.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Veterinary Sciences
G. M. Mund, T. C. Haeussler, S. H. Aurich, C. Ewers, C. Thiel
Summary: This case involved a 7-year-old neutered female Labrador Retriever from Germany with peritoneal nodular masses caused by Nocardia paucivorans. Surgical management and antimicrobial therapy were effective in treating the infection, with no recurrence after 27 months.
NEW ZEALAND VETERINARY JOURNAL
(2021)
Article
Environmental Sciences
Ursula Siebert, Julian Sturznickel, Tobias Schaffeld, Ralf Oheim, Tim Rolvien, Ellen Prenger-Berninghoff, Peter Wohlsein, Jan Lakemeyer, Simon Rohner, Luca Aroha Schick, Stephanie Gross, Dominik Nachtsheim, Christa Ewers, Paul Becher, Michael Amling, Maria Morell
Summary: Harbour porpoises are facing pressure from increasing human activities, including detonation of wartime ammunition. A study found evidence of blast injuries in porpoises following the clearance of WWII mines. The data highlights the importance of systematic investigations into the effects of blast and acoustic trauma in these marine mammals.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Agriculture, Dairy & Animal Science
Simon Rohner, Peter Wohlsein, Ellen Prenger-Berninghoff, Christa Ewers, Patrick Waindok, Christina Strube, Christine Baechlein, Paul Becher, Dunja Wilmes, Volker Rickerts, Ursula Siebert
Summary: Working with dead otters can provide valuable insights for population health investigations. In Schleswig-Holstein, Germany, most of the dead otters found were males aged between 1-3 years with good nutritional status. Roadkill was a major cause of death, and post-mortem investigations are essential for analyzing potential trends and establishing a monitoring program for Eurasian otters in the region.
Article
Microbiology
Linda Fenske, Irene Noll, Jochen Blom, Christa Ewers, Torsten Semmler, Ahmad Fawzy, Tobias Eisenberg
Summary: Bovine mastitis caused by Streptococcus uberis is a common problem in the dairy industry, but the severity of clinical symptoms can vary greatly. This study conducted a comparative genomic analysis of S. uberis isolates from different infection courses, revealing unknown factors responsible for the severity and highlighting the importance of further research on the pathogenicity of S. uberis.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2022)
Article
Virology
Ahmed Lugelo, Katie Hampson, Elaine A. Ferguson, Anna Czupryna, Machunde Bigambo, Christian Tetteh Duamor, Rudovick Kazwala, Paul C. D. Johnson, Felix Lankester
Summary: This study describes a novel approach to improve vaccination coverage and maintain herd immunity against rabies through decentralized and continuous vaccination delivery.
Article
Microbiology
Franziska Unger, Tobias Eisenberg, Ellen Prenger-Berninghoff, Ursula Leidner, Torsten Semmler, Christa Ewers
Summary: A study on live reptiles revealed the presence of potentially resistant Acinetobacter spp. strains, with both new and existing resistance mechanisms. The study also found a high variation in the Acinetobacter spp. community in reptiles, with many strains displaying multidrug resistance.
Article
Infectious Diseases
Lisa Jacobmeyer, Torsten Semmler, Ivonne Stamm, Christa Ewers
Summary: Carbapenemase-producing Acinetobacter baumannii has been identified in veterinary clinics in Germany, with different resistance gene patterns and potential transmission within the veterinary environment.
Article
Microbiology
Marcus Faehndrich, Jana C. Klink, Marco Roller, Peter Wohlsein, Katharina Raue, Christina Strube, Ellen Prenger-Berninghoff, Christa Ewers, Lorenzo Capucci, Antonio Lavazza, Herbert Tomaso, Joseph G. Schnitzler, Ursula Siebert
Summary: The study reveals that infectious diseases are a major contributing factor to the decline in the population of European brown hares in Schleswig-Holstein, Germany. Pathological examination showed that hepatitis, pneumonia, nephritis, liver necrosis, and enteritis were among the main findings. The study also highlights the high mortality and potential zoonotic risks associated with various infectious pathogens.
Article
Infectious Diseases
Manuela Tietgen, Lisa Sedlaczek, Paul G. G. Higgins, Heike Kaspar, Christa Ewers, Stephan Goettig
Summary: This study explores common colistin resistance mechanisms in Klebsiella pneumoniae isolates from animals, finding that the resistance mechanisms were diverse alterations of MgrB in both human and animal isolates. Phylogenetic analysis indicated that colistin resistance mechanisms developed independently in human and animal isolates. Further analysis of resistance mechanisms is crucial due to the limited antibiotics available to treat infections with multidrug-resistant bacteria.
Article
Infectious Diseases
Sophie Aurich, Ellen Prenger-Berninghoff, Christa Ewers
Summary: This study analyzed urinary tract samples from dogs and cats in Germany and determined the susceptibility of uropathogenic isolates to different antimicrobials. The results showed that Escherichia coli was the most frequently isolated pathogen and females were more likely to have positive microbiological cultures. Empiric treatment of UTI with amoxicillin/clavulanic acid or trimethoprim-sulfamethoxazole is recommended.
Article
Microbiology
Maria Borowiak, Antonia Kreitlow, Burkhard Malorny, Mazen Alssahen, Christoph Laemmler, Ellen Prenger-Berninghoff, Christa Ewers, Ursula Siebert, Madeleine Ploetz, Amir Abdulmawjood
Summary: The genus Arcanobacterium continues to expand with the identification of novel species. Harbor seals have been found to commonly carry bacteria from this genus. In this study, we report the complete genome sequence of another Arcanobacterium species, specifically Arcanobacterium pinnipediorum strain DSM 28752, isolated from a harbor seal.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Johanna Ruehl-Teichner, Lisa Jacobmeyer, Ursula Leidner, Torsten Semmler, Christa Ewers
Summary: This study investigated 78 isolates of Acinetobacter baumannii from horses and found that they displayed high genetic diversity, the ability to form biofilms, and resistance to multiple antimicrobial drugs. The presence of globally distributed dominant clones was also identified. These findings are important for understanding treatment failures and limited availability of licensed drugs in equine medicine.
Article
Microbiology
Marcus Faehndrich, Benno Woelfing, Jana C. Klink, Marco Roller, Wolfgang Baumgaertner, Peter Wohlsein, Katharina Raue, Christina Strube, Christa Ewers, Ellen Prenger-Berninghoff, Jutta Verspohl, Antonio Lavazza, Lorenzo Capucci, Herbert Tomaso, Ursula Siebert
Summary: By analyzing samples from European brown hares in three locations in the northernmost German state of Schleswig-Holstein, it was found that infectious diseases have a relatively low importance on the observed population dynamics, but there is a high prevalence of chronic inflammations of unknown origin.
Article
Veterinary Sciences
Dordia Anindita Rotinsulu, Christa Ewers, Katharina Kerner, Amrozi Amrozi, Retno Damayanti Soejoedono, Torsten Semmler, Rolf Bauerfeind
Summary: This study aimed to characterize S. equi equi isolates obtained from suspected strangles cases in Indonesia in 2018. The results showed that all isolates belonged to ST179 and carried seM allele 166. The isolates differed from each other by only 2 to 14 cgSNPs and formed an exclusive sub-cluster within the BAPS-2 of S. equi equi cgMLST scheme. Furthermore, all isolates were susceptible to beta-lactam antibiotics penicillin G, ampicillin, and ceftiofur.
VETERINARY SCIENCES
(2023)
