Article
Veterinary Sciences
Shengnan Wang, Yanchao Duan, Bingbing Chen, Shuai Qiu, Tianzhuang Huang, Wei Si
Summary: Gene editing technology is considered an effective way to establish animal models of human diseases. Using spermatogonial stem cells and sperm as gene editing objects is simple to operate. However, there is no research on gene editing of spermatogonial stem cells to obtain genetically modified sperm, and it is unknown whether cryopreservation has adverse effects on genetically modified sperm. Furthermore, clear cell surface markers to identify primate spermatogonial stem cells are lacking. In this experiment, the enhanced green fluorescent protein lentivirus was injected into seminiferous tubules through the testis reticulum using ultrasound guidance, resulting in the successful production of transgenic sperm with similar freezing and recovery rates to wild animals.
VETERINARY SCIENCES
(2023)
Article
Cell Biology
Fan Yang, Eoin C. Whelan, Xuebing Guan, Bingquan Deng, Shu Wang, Jiachen Sun, Mary R. Avarbock, Xin Wu, Ralph L. Brinster
Summary: Overexpression of Fgf9 in vivo results in arrested spermatogenesis and accumulation of undifferentiated spermatogonia; exposure of germ cell cultures to FGF9 leads to increased numbers of SSCs over time; inhibition of p38 MAPK phosphorylation negates the growth advantage provided by FGF9.
CELL PROLIFERATION
(2021)
Article
Zoology
Cong Li, Rui Bi, Lin Wang, Yu-Hua Ma, Yong-Gang Yao, Ping Zheng
Summary: In this study, the in vivo spermatogenesis potential of tree shrew SSCs cultured across different passages was examined. It was found that long-term expansion (>50 passages) led to the loss of spermatogenesis ability in SSCs. RNA sequencing analysis revealed significant gene expression changes after sub-culturing primary SPG lines for more than 40 passages, and experimental validation confirmed DNA damage accumulation and mitochondrial dysfunction in high-passage cells. Supplementation with NAD(+) precursor NR showed beneficial effects in reducing DNA damage accumulation and mitochondrial dysfunction. This research provides a comprehensive analysis of the genetic and physiological attributes critical for the sustained expansion of undifferentiated SSCs in tree shrews and proposes an effective strategy for extended in vitro maintenance.
ZOOLOGICAL RESEARCH
(2023)
Article
Veterinary Sciences
Xuejiao An, Qiao Li, Nana Chen, Taotao Li, Huihui Wang, Manchun Su, Huibin Shi, Youji Ma
Summary: This study established a co-culture system of SCs and SSCs and identified a list of genes and small metabolic molecules that affect the proliferation and differentiation of SSCs. The findings provide additional insights into the regulatory mechanisms underlying interactions between SCs and SSCs during mammalian spermatogenesis.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Medicine, General & Internal
D. B. Kohn, C. Booth, K. L. Shaw, J. Xu-Bayford, E. Garabedian, V. Trevisan, D. A. Carbonaro-Sarracino, K. Soni, D. Terrazas, K. Snell, A. Ikeda, D. Leon-Rico, T. B. Moore, K. F. Buckland, A. J. Shah, K. C. Gilmour, S. De Oliveira, C. Rivat, G. M. Crooks, N. Izotova, J. Tse, S. Adams, S. Shupien, H. Ricketts, A. Davila, C. Uzowuru, A. Icreverzi, P. Barman, B. Campo Fernandez, R. P. Hollis, M. Coronel, A. Yu, K. M. Chun, C. E. Casas, R. Zhang, S. Arduini, F. Lynn, M. Kudari, A. Spezzi, M. Zahn, R. Heimke, I. Labik, R. Parrott, R. H. Buckley, L. Reeves, K. Cornetta, R. Sokolic, M. Hershfield, M. Schmidt, F. Candotti, H. L. Malech, A. J. Thrasher, H. B. Gaspar
Summary: Gene therapy for ADA deficiency in 50 patients in the United States and the United Kingdom resulted in high overall survival and event-free survival rates, sustained metabolic correction, and functional immune reconstitution. No adverse events such as replication-competent virus, abnormal proliferations, autoimmunity, or graft-versus-host disease were observed.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Oncology
Lei Wang, Jiang Long, Huan Chen, Shaoyang Sun, Kunpeng Lv, Qiang Li, Xu Wang
Summary: The study demonstrates a novel approach to therapeutically target specific focuses by inhibiting aberrant Wnt activity without interfering with normal tissues using synthetic cell-based therapy. Successful proof of principle in an in vivo model suggests the potential of combining synthetic cells with antigen receptors to enhance treatment accuracy.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Obstetrics & Gynecology
Wei Le, Fang Zhou, Jun Xiang, Yiming Weng, Denglong Wu, Jun Xu, Jinfu Zhang
Summary: This study reveals the specific mechanism of 53BP1 in the DNA damage repair process of germline stem cells, providing new theoretical basis and insights for the diagnosis and treatment of male infertility.
REPRODUCTIVE SCIENCES
(2023)
Article
Cell Biology
Dai Zhou, Jingyu Fan, Zhizhong Liu, Ruiling Tang, Xingming Wang, Hao Bo, Fang Zhu, Xueheng Zhao, Zenghui Huang, Liu Xing, Ke Tao, Han Zhang, Hongchuan Nie, Huan Zhang, Wenbing Zhu, Zuping He, Liqing Fan
Summary: This study reveals that TCF3 regulates the proliferation and apoptosis of human SSCs via targeting PODXL, providing a novel molecular mechanism for understanding the fate determinations of human SSCs and potential new targets for gene therapy of male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Dai Zhou, Jingyu Fan, Zhizhong Liu, Ruiling Tang, Xingming Wang, Hao Bo, Fang Zhu, Xueheng Zhao, Zenghui Huang, Liu Xing, Ke Tao, Han Zhang, Hongchuan Nie, Huan Zhang, Wenbing Zhu, Zuping He, Liqing Fan
Summary: The gene TCF3 was found to promote proliferation and inhibit apoptosis of human SSCs by targeting PODXL, offering new targets for gene therapy of male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Endocrinology & Metabolism
Lei Diao, Paul J. Turek, Constance M. John, Fang Fang, Renee A. Reijo Pera
Summary: Spermatogonial stem cells (SSCs) are essential adult stem cells involved in spermatogenesis and male fertility. Understanding the regulation of SSCs is crucial for studying their function and the causes of male infertility, as well as developing new treatments through in vitro culture and differentiation of SSCs. This review highlights the recent research progress on SSC regulation and discusses the potential application of SSCs in fertility restoration.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Xiaomin Du, Siyu Wu, Yudong Wei, Xiuwei Yu, Fanglin Ma, Yuanxin Zhai, Donghui Yang, Mengfei Zhang, Wenqing Liu, Haijing Zhu, Jiang Wu, Mingzhi Liao, Na Li, Chunling Bai, Guangpeng Li, Jinlian Hua
Summary: The study identified a subset of spermatogonial stem cells in dairy goats with high PAX7 expression, indicating that PAX7 can maintain the self-renewal of these cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Mengfei Zhang, Na Li, Wenqing Liu, Xiaomin Du, Yudong Wei, Donghui Yang, Zhe Zhou, Fanglin Ma, Sha Peng, Shiqiang Zhang, Xin He, Chunling Bai, Guangpeng Li, Jinlian Hua
Summary: The study revealed an important role of Eif2s3y in dairy goat reproduction, promoting the proliferation of spermatogonial stem cells by activating the ERK signaling pathway and generating more colonies in infertile mice. This finding provides a crucial basis for future research on male spermatogenic cell restoration.
STEM CELLS INTERNATIONAL
(2021)
Article
Physiology
Kesong Shi, Baoluri Wang, Le Dou, Shu Wang, Xinrui Fu, Haiquan Yu
Summary: Through the analysis of multiple datasets, we identified SSC-specific transcription factors and core genes, and revealed the complex regulatory network of these factors and genes in the process of SSC formation. These findings are of great significance for improving the induction efficiency of ESC differentiation into SSCs in vitro.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Yu Lin, Qian Fang, Yue He, Xiaowen Gong, Yinjuan Wang, Ajuan Liang, Guishuan Wang, Shengnan Gong, Ji Wu, Fei Sun
Summary: The study revealed the presence of extracellular vesicles in the basal compartment of seminiferous tubules in mice, rats, rabbits, and humans, which are secreted by spermatogonia and taken up by SSCs. These extracellular vesicles purified from Thy1-positive spermatogonia were found to suppress the proliferation of SSCs without inducing apoptosis.
Article
Endocrinology & Metabolism
Yu Lin, Qian Fang, Yue He, Xiaowen Gong, Yinjuan Wang, Ajuan Liang, Guishuan Wang, Shengnan Gong, Ji Wu, Fei Sun
Summary: This study identified the presence of extracellular vesicles in the basal compartment of mammalian seminiferous tubules, and demonstrated their role in controlling the proliferation of spermatogonial stem cells without inducing apoptosis. It sheds light on the potential regulatory mechanisms of SSCs by extracellular vesicles.
Article
Hematology
Felipe Samaniego, Fredrick Hagemeister, Jorge E. Romaguera, Michelle A. Fanale, Barbara Pro, Peter McLaughlin, M. Alma Rodriguez, Sattva S. Neelapu, Luis Fayad, Anas Younes, Lei Feng, Zuzana Berkova, Tamer Khashab, Lalit Sehgal, Francisco Vega-Vasquez, Larry W. Kwak
BRITISH JOURNAL OF HAEMATOLOGY
(2015)
Article
Oncology
Rohit Mathur, Lalit Sehgal, Frank K. Braun, Zuzana Berkova, Jorge Romaguerra, Michael Wang, M. Alma Rodriguez, Luis Fayad, Sattva S. Neelapu, Felipe Samaniego
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2015)
Article
Oncology
Haifeng Zhu, Zuzana Berkova, Rohit Mathur, Lalit Sehgal, Tamer Khashab, Rong-Hua Tao, Xue Ao, Lei Feng, Anita L. Sabichi, Boris Blechacz, Asif Rashid, Felipe Samaniego
MOLECULAR CANCER RESEARCH
(2015)
Article
Hematology
Rohit Mathur, Lalit Sehgal, Ondrej Havranek, Stefan Kohrer, Tamer Khashab, Neeraj Jain, Jan A. Burger, Sattva S. Neelapu, R. Eric Davis, Felipe Samaniego
Article
Medicine, Research & Experimental
Xin Wang, Lalit Sehgal, Neeraj Jain, Tamer Khashab, Rohit Mathur, Felipe Samaniego
JOURNAL OF TRANSLATIONAL MEDICINE
(2016)
Article
Multidisciplinary Sciences
Amitabha Mukhopadhyay, Lalit Sehgal, Arunabha Bose, Anushree Gulvady, Parijat Senapati, Rahul Thorat, Srikanta Basu, Khyati Bhatt, Amol S. Hosing, Renu Balyan, Lalit Borde, Tapas K. Kundu, Sorab N. Dalal
SCIENTIFIC REPORTS
(2016)
Article
Hematology
Rohit Mathur, Lalit Sehgal, Ondrej Havranek, Stefan Kohrer, Tamer Khashab, Neeraj Jain, Jan A. Burger, Sattva S. Neelapu, R. Eric Davis, Felipe Samaniego
Article
Oncology
N. Jain, H. Zhu, T. Khashab, Q. Ye, B. George, R. Mathur, R. K. Singh, Z. Berkova, J. F. Wise, F. K. Braun, X. Wang, K. Patel, Zy Xu-Monette, J. Courty, K. H. Young, L. Sehgal, F. Samaniego
Article
Biochemistry & Molecular Biology
Mansa Gurjar, Kumarkrishna Raychaudhuri, Snehal Mahadik, Divya Reddy, Apurva Atak, Trupti Shetty, Kruthi Rao, Mansi S. Karkhanis, Prajakta Gosavi, Lalit Sehgal, Sanjay Gupta, Sorab N. Dalal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2018)
Article
Oncology
Carolina Vicente-Duenas, Ines Gonzalez-Herrero, Lalit Sehgal, Idoia Garcia-Ramirez, Guillermo Rodriguez-Hernandez, Belen Pintado, Oscar Blanco, Francisco Javier Garcia Criado, Maria Begona Garcia Cenador, Michael R. Green, Isidro Sanchez-Garcia
Review
Biochemistry & Molecular Biology
Anuvrat Sircar, Sayan Mullick Chowdhury, Amber Hart, William Connor Bell, Satishkumar Singh, Lalit Sehgal, Narendranath Epperla
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Oncology
Bhawana George, Sayan Mullick Chowdhury, Amber Hart, Anuvrat Sircar, Satish Kumar Singh, Uttam Kumar Nath, Mukesh Mamgain, Naveen Kumar Singhal, Lalit Sehgal, Neeraj Jain
Article
Oncology
Fouad Choueiry, Satishkumar Singh, Anuvrat Sircar, Georgios Laliotis, Xiaowei Sun, Evangelia Chavdoula, Shiqi Zhang, JoBeth Helmig-Mason, Amber Hart, Narendranath Epperla, Philip Tsichlis, Robert Baiocchi, Lapo Alinari, Jiangjiang Zhu, Lalit Sehgal
Summary: This study investigates the modulator of ibrutinib resistance in ABC diffuse large B-cell lymphoma by integrating Metabolomics and Gene expression profiling, revealing key genes and pathways driving metabolic deregulation in cancer cells. The findings highlight the role of metabolic reprogramming during drug resistance development, and how drug-resistant clones shift towards oxidative phosphorylation modulated via the BTK-PI3K-AKT-IL4I1 axis.
Article
Anesthesiology
Lalit Sehgal, Piyush Srivastava, Chandra Kant Pandey, Amit Jha
INDIAN JOURNAL OF ANAESTHESIA
(2016)
Article
Cell Biology
Zuzana Berkova, Shu Wang, Lalit Sehgal, Keyur Pravinchandra Patel, Om Prakash, Felipe Samaniego
HISTOLOGY AND HISTOPATHOLOGY
(2015)
