Article
Biotechnology & Applied Microbiology
Irina Charlot Pena-Moreno, Denise Castro Parente, Karolini Miranda da Silva, Elton Pedro Nunes Pena, Fabiana Aparecida Cavalcante Silva, Tercilio Calsa Junior, Will de Barros Pita, Marcos Antonio de Morais Jr
Summary: The yeast Dekkera bruxellensis exhibits superior anaerobic growth and ethanol yield in the presence of nitrate, due to balanced activation of TORC1 and NCR de-repression mechanisms regulating cell metabolism. On the other hand, poor growth in aerobiosis is likely caused by oxidative stress triggered by nitrate when oxygen is present.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Reproductive Biology
Xiaohong Yao, Chaofan Wang, Longjie Sun, Lu Yan, Xuexue Chen, Zheng Lv, Xiaomei Xie, Shuang Tian, Wenbo Liu, Lei Li, Hua Zhang, Jiali Liu
Summary: In this study, it was discovered that BCAS2 protein regulates pre-mRNA splicing in granulosa cells, affecting their proliferation and survival. BCAS2 participates in the PRP19 complex to mediate alternative splicing of E2f3 and Flt3l mRNA, thereby inhibiting the cell cycle. These findings reveal the function of BCAS2 in granulosa cells.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Clarisse van der Feltz, Brandon Nikolai, Charles Schneider, Joshua C. Paulson, Xingyang Fu, Aaron A. Hoskins
Summary: Spliceosome catalysis is influenced by nonessential components, with Ecm2 playing a role in stabilizing catalytic sites and promoting alternate splice site usage. The interaction between Cwc2 and Ecm2 can fine-tune the spliceosome active site in unique ways, allowing for regulation of splicing of pre-mRNAs containing weak or alternate splice sites.
Review
Plant Sciences
Praveen Kumar Kathare, Enamul Huq
Summary: The article summarizes the role of phytochromes in regulating plant growth and development, particularly in the regulation of pre-mRNA splicing process.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sierra L. L. Love, Joseph D. D. Emerson, Kazunori Koide, Aaron A. A. Hoskins
Summary: Pre-mRNA splicing is a crucial step in human gene expression, carried out by a macromolecular machine known as the spliceosome. Mutations in this machinery can lead to various human diseases. This review provides an overview of the main features of the pre-mRNA splicing machinery in humans and how changes in its components' function can result in diseases ranging from blindness to cancers. The article also discusses drugs that interact with the spliceosome to alter splicing outcomes and speculates on future treatments for spliceosomopathies.
Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Christophe Rachez, Rachel Legendre, Mickael Costallat, Hugo Varet, Jia Yi, Etienne Kornobis, Christian Muchardt
Summary: HP1 proteins, known for marking heterochromatin and gene silencing, are also RNA-binding proteins. HP1 gamma, in particular, is targeted to hexameric RNA motifs and SINE transposable elements, tethering unspliced pre-mRNA to chromatin through intronic regions and limiting the usage of intronic cryptic splice sites during transcription. This reveals new insights into the relationship between chromatin and co-transcriptional splicing.
Review
Oncology
Sunkyung Choi, Namjoon Cho, Eun-Mi Kim, Kee K. Kim
Summary: Alternative pre-mRNA splicing plays a critical role in generating multiple mRNA and increasing protein diversity. Specific splicing isoforms have been found to be important in cellular processes, particularly in regulating cell numbers. Abnormal expression of splicing isoforms and factors, as well as disruptions in splicing caused by genetic mutations, are implicated in cancer development and progression. Alternative splicing has potential as a therapeutic target for cancer.
CANCER CELL INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Praveen Kumar Kathare, Ruijiao Xin, Abirama Sundari Ganesan, Viviana M. June, Anireddy S. N. Reddy, Enamul Huq
Summary: Light signals have significant effects on the physiology, growth, and development of plants. SWAP1, an RNA-binding splicing factor, regulates light-regulated alternative splicing by interacting with other splicing factors and photoactivated phyB.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Jennifer E. Hurtig, Michelle A. Steiger, Vinay K. Nagarajan, Tao Li, Ti-Chun Chao, Kuang-Lei Tsai, Ambro van Hoof
Summary: Recent studies show that yeast TSEN cleaves a specific subset of mRNAs encoding mitochondrial proteins, with cleavage sites determined by sequence. The development of the comPARE bioinformatic approach enables the identification of these mRNA target sites more effectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Yi Zhang, Haoting Chen, Shuo Li, Yang Li, Mukesh Kumar Kanwar, Bin Li, Longqiang Bai, Jin Xu, Yu Shi
Summary: The study showed that exogenous BR can enhance chlorophyll metabolism and adjust osmotic stress responses in hydroponically grown tomatoes under Ca(NO3)(2) stress, thereby alleviating the impact of stress on plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Yang Pan, John W. Phillips, Beatrice D. Zhang, Miyako Noguchi, Eric Kutschera, Jami McLaughlin, Pavlo A. Nesterenko, Zhiyuan Mao, Nathanael J. Bangayan, Robert Wang, Wendy Tran, Harry T. Yang, Yuanyuan Wang, Yang Xu, Matthew B. Obusan, Donghui Cheng, Alex H. Lee, Kathryn E. Kadash-Edmondson, Ameya Champhekar, Cristina Puig-Saus, Antoni Ribas, Robert M. Prins, Christopher S. Seet, Gay M. Crooks, Owen N. Witte, Yi Xing
Summary: This study describes a computational platform called IRIS that can discover tumor antigens derived from alternative splicing, providing potential targets for TCR and CAR-T immunotherapies. Through the analysis of transcriptomics and immuno-peptidomics data, the study demonstrates that the predicted targets by IRIS can bind with HLA molecules. The study illustrates the contribution of alternative splicing to the repertoire of tumor antigens and demonstrates the utility of IRIS in discovering AS-derived antigens and expanding cancer immunotherapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Oncology
Shinsuke Araki, Momoko Ohori, Masato Yugami
Summary: This review summarizes recent progress in the relationship between druggable splicing-related molecules and cancer, highlights small-molecule splicing modulators, and discusses future perspectives of splicing modulation for personalized and combination therapies in cancer treatment.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Changshou Wang, Lin Xu, Chen Du, Hao Yun, Keyun Wang, Hui Liu, Mingliang Ye, Jing Fan, Yu Zhou, Hong Cheng
Summary: CDK11 is a potential druggable target for cancer therapy, as it plays important roles in phosphorylating transcription and splicing factors and facilitating cell cycle progression in cancer cells. This study reveals that CDK11 forms a tight complex with cyclins L1/L2 and SAP30BP, and that SAP30BP is a critical activator of CDK11, regulating global pre-mRNA splicing.
Article
Biochemistry & Molecular Biology
Florence Schlotter, Salim Merouani, Justine Flayac, Valentyne Kogey, Amani Issa, Maxime Dodre, Alexandra Huttin, Christiane Branlant, Edouard Bertrand, Stephane Labialle, Franck Vandermoere, Celine Verheggen, Severine Massenet
Summary: The assembly of H/ACA RNPs involves several assembly factors and the formation of distinct intermediate complexes. Our study reveals the mechanism of H/ACA RNP assembly and identifies new proteins associated with its assembly or function.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Genetics & Heredity
Christopher J. Buehl, Xiexiong Deng, Jianjun Luo, Visarut Buranasudja, Tony Hazbun, Min-Hao Kuo
Article
Genetics & Heredity
Christopher J. Buehl, Xiexiong Deng, Jianjun Luo, Visarut Buranasudja, Tony Hazbun, Min-Hao Kuo
Article
Cell Biology
Alaina H. Willet, K. Adam Bohnert, Kathleen L. Gould
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Cell Biology
Zachary C. Elmore, Rodrigo X. Guillen, Kathleen L. Gould
MOLECULAR BIOLOGY OF THE CELL
(2018)
Review
Genetics & Heredity
Sierra N. Cullati, Kathleen L. Gould
Article
Cell Biology
K. Adam Bohnert, Anthony M. Rossi, Quan-Wen Jin, Jun-Song Chen, Kathleen L. Gould
JOURNAL OF CELL SCIENCE
(2020)
Article
Biochemical Research Methods
Panyue Chen, Tiago Jose Paschoal Sobreira, Mark C. Hall, Tony R. Hazbun
Summary: The study reanalyzed proteomic datasets to explore the extent of alpha-N-terminal methylation in yeast and humans, finding evidence of canonical and noncanonical sequences being methylated. A unique amino acid frequency pattern was observed for the methylated peptides in yeast and humans, differing from the canonical motif. This study demonstrates the feasibility of using proteomic data for global investigations into alpha-N-terminal methylation.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jun-Song Chen, Christine M. Jones, Maya G. Igarashi, Liping Ren, Alyssa E. Johnson, Kathleen L. Gould
Summary: Preventing phosphorylation of Dma1 reduces its localization to the contractile ring, but does not affect its function in the mitotic checkpoint, highlighting the importance of Dma1 localization to spindle pole bodies for its role in the checkpoint.
Article
Biology
Rahul Bhattacharjee, Aaron R. Hall, MariaSanta C. Mangione, Maya G. Igarashi, Rachel H. Roberts-Galbraith, Jun-Song Chen, Dimitrios Vavylonis, Kathleen L. Gould
Summary: The F-BAR protein Cdc15 is crucial for cytokinesis in yeast and is involved in attaching the cytokinetic ring to the plasma membrane. Various cell polarity kinases, particularly Pom1, regulate the phosphorylation of Cdc15, which in turn affects its localization and ability to interact with the membrane. Coarse-grained simulations suggest that phosphorylation prevents F-BAR oligomerization and membrane interaction, and dephosphorylated Cdc15 undergoes liquid-liquid phase separation to form condensates.
Article
Biology
Anthony M. Rossi, Kathleen L. Gould, K. Adam Bohnert
Summary: This study explored the role of the cytokinetic ring component Fic1 in septum formation. It was found that Fic1 promotes septum formation by interacting with F-BAR proteins Cdc15 and Imp2, as well as with Cyk3. The findings indicate differences in the mechanisms of cell division in different yeasts.
Article
Genetics & Heredity
Alaina H. Willet, Marcin Wos, Maya G. Igarashi, Liping Ren, Lesley A. Turner, Kathleen L. Gould
Summary: Coupling cell wall expansion with cell growth is a universal challenge faced by walled organisms. Changes in plasma membrane lipid composition and dynamics, specifically related to sphingolipids, affect cell wall material accumulation and cell growth in S. pombe.
Article
Genetics & Heredity
Manuel Lera-Ramirez, Jurg Bahler, Juan Mata, Kim Rutherford, Charles S. Hoffman, Sarah Lambert, Snezhana Oliferenko, Sophie G. Martin, Kathleen L. Gould, Li-Lin Du, Sarah A. Sabatinos, Susan L. Forsburg, Olaf Nielsen, Paul Nurse, Valerie Wood
Summary: Standardized nomenclature for genes, gene products, and isoforms is crucial for clear communication and efficient sharing of scientific data. This publication extends the fission yeast clade gene nomenclature guidelines to support curation efforts at PomBase, introducing guidelines for noncoding RNA genes and updating allele and genotype nomenclature. Adoption of these rules will improve consistency in gene and genotype nomenclature and enhance machine-readability in publications or datasets.
Article
Biochemistry & Molecular Biology
Abigail M. Brown, Lindsay C. Meyers, Janani Varadarajan, Nicholas J. Ward, Jean-Philippe Cartailler, Roger G. Chalkley, Kathleen L. Gould, Kimberly A. Petrie
Summary: This study investigates the career goals and outcomes of 1452 biomedical sciences PhDs who graduated from Vanderbilt University between 1997 and 2021. The findings show that most students change their career goals during graduate school, fewer alumni pursue postdoctoral training, and many alumni enter their first non-training positions in a different career area than their original goal at doctoral defense.
Article
Immunology
Hassan E. Eldesouky, Nadia A. Lanman, Tony R. Hazbun, Mohamed N. Seleem
Article
Microbiology
Hassan E. Eldesouky, Abdelrahman Mayhoub, Tony R. Hazbun, Mohamed N. Seleem
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)